Different Cell Disruption and Lipid Extraction Methods from Microalgae for Biodiesel Production

2019 ◽  
pp. 265-292 ◽  
Author(s):  
Vinod Kumar ◽  
Neha Arora ◽  
Manisha Nanda ◽  
Vikas Pruthi
Keyword(s):  
Lipid Extraction ◽  
Extraction Methods ◽  
Cell Disruption ◽  
Biodiesel Production

Biodiesel Production of Amphora sp. and Navicula sp. by Different Cell Disruption and Lipid Extraction Methods

2015 ◽  
Vol 9 (6) ◽  
pp. 588-595
Author(s):  
Haifa Chtourou ◽  
Ines Dahmen ◽  
Fatma Karray ◽  
Sami Sayadi ◽  
Abdelhafidh Dhouib
Keyword(s):  
Lipid Extraction ◽  
Extraction Methods ◽  
Cell Disruption ◽  
Biodiesel Production

scholarly journals Physical Methods of Microalgal Biomass Pretreatment

2014 ◽  
Vol 28 (3) ◽  
pp. 341-348 ◽  
Author(s):  
Agata Piasecka ◽  
Izabela Krzemińska ◽  
Jerzy Tys
Keyword(s):  
Alternative Energy ◽  
Lipid Extraction ◽  
Physical Method ◽  
Extraction Methods ◽  
Biodiesel Production ◽  
Biomass Pretreatment ◽  
Microalgal Biomass ◽  
Alternative Energy Sources ◽  
Wet Biomass ◽  
Microwave Techniques

Abstract The prospect of depletion of natural energy resources on the Earth forces researchers to seek and explore new and alternative energy sources. Biomass is a composite resource that can be used in many ways leading to diversity of products. Therefore, microalgal biomass offers great potential. The main aim of this study is to find the best physical method of microalgal biomass pretreatment that guarantees efficient lipid extraction. These studies identifies biochemical composition of microalgal biomass as source for biodisel production. The influence of drying at different temperatures and lyophilization was investigated. In addition, wet and untreated biomass was examined. Cell disruption (sonication and microwave) techniques were used to improve lipid extraction from wet biomass. Additionally, two different extraction methods were carried out to select the best method of crude oil extraction. The results of this study show that wet biomass after sonication is the most suitable for extraction. The fatty acid composition of microalgal biomass includes linoleic acid (C18:2), palmitic acid (C16:0), oleic acid (C18:1), linolenic acid (C18:3), and stearic acid (C18:0), which play a key role in biodiesel production.

Microalgae harvesting and cell disruption: a preliminary evaluation of the technology electroflotation by alternating current

2014 ◽  
Vol 70 (2) ◽  
pp. 315-320 ◽  
Author(s):  
Riamburgo Gomes de Carvalho Neto ◽  
José Gilmar da Silva do Nascimento ◽  
Mayara Carantino Costa ◽  
Alexandre Colzi Lopes ◽  
Eliezer Fares Abdala Neto ◽  
...  
Keyword(s):  
Lipid Extraction ◽  
Alternating Current ◽  
Cell Disruption ◽  
Biodiesel Production ◽  
Attractive Potential ◽  
Preliminary Evaluation ◽  
Waste Stabilization Ponds ◽  
High Productivity ◽  
Microalgae Harvesting ◽  
Batch Time

Some species of microalgae have high productivity and lipid content, which makes them good candidates for biodiesel production. Biomass separation and cell disruption are important steps in biodiesel production from microalgae. In this work, we explored the fundamentals of electroflotation by alternating current (EFAC) with non-consumable electrodes to simultaneously harvest microalgae and disrupt cells from mixed microalgae obtained from waste stabilization ponds. The harvesting efficiency was evaluated using chlorophyll-a and turbidity, which reached removals of 99% and 95%, respectively, during a batch time of 140 min. Cell disruption was evaluated using lipid extraction, and the best results were achieved with a batch time of 140 min, which resulted in a 14% yield. Therefore, EFAC was shown to be an attractive potential technology for simultaneous microalgal harvesting and cell disruption.

Comparison of cell disruption techniques prior to lipid extraction from Scenedesmus sp. slurries for biodiesel production using liquid CO2

2018 ◽  
Vol 20 (18) ◽  
pp. 4330-4338 ◽  
Author(s):  
K. J. Viner ◽  
P. Champagne ◽  
P. G. Jessop
Keyword(s):  
Microwave Radiation ◽  
Lipid Extraction ◽  
Cell Disruption ◽  
Biodiesel Production ◽  
Liquid Co2

Microwave radiation exhibits the highest potential for releasing lipids from Scenedesmus sp.

scholarly journals Comparison of Nannochloropsis sp. cells disruption between hydrodynamic cavitation and conventional extraction

2018 ◽  
Vol 154 ◽  
pp. 01023 ◽  
Author(s):  
Martomo Setyawan ◽  
Panut Mulyono ◽  
Sutijan ◽  
Arief Budiman
Keyword(s):  
Mass Transfer ◽  
Transfer Coefficient ◽  
Distribution Coefficient ◽  
Mass Transfer Coefficient ◽  
Lipid Extraction ◽  
Cell Disruption ◽  
Hydrodynamic Cavitation ◽  
Biodiesel Production ◽  
Experimental Result ◽  
Conventional Extraction

Biodiesel production from microalgae is one of the solution of the future energy problem, but its production cost is still high. One of the costly stages of this process is the lipid extraction process. It can be reduced by microalgae cell disruption. One of the mechanical method to cell disruption with the lowest energy requirement is hydrodynamic cavitation. This aim of this study is to evaluate the distribution coefficient and the mass transfer coefficient value of lipid extraction of Nannochloropsis sp. assisted by hydrodynamic cavitation and compare with conventional extraction. The hydrodynamic cavitation extraction was done at 34 °C, 1 atm. The conventional extraction was done at 34 °C, 1 atm with stirring speed 260 and 1000 rpm. The experimental result shows that the distribution coefficient dependent on the temperature with the values for 50, 44, 38 and 34 °C were 0.502, 0.394, 0.349, and 0.314 respectively. And it was according to Van’ Hoff equation with the values of ΔH° was 20.718 kJ/mol and ΔS° was 58.05 J/mol/K. The hydrodynamic cavitation extraction was faster than conventional. The mass transfer coefficient values for hydrodynamic cavitation, conventional 260 rpm and 1000 rpm were 7.373, 0.534 and 0.121 1/s respectively.

scholarly journals Optimization of cell wall disruption and lipid extraction methods by combining different solvents from wet microalgae

2021 ◽  
Author(s):  
Daryush Arabian
Keyword(s):  
Cell Wall ◽  
Lipid Extraction ◽  
Dry Weight ◽  
Extraction Methods ◽  
Biodiesel Production ◽  
Cell Wall Disruption ◽  
Bead Mill ◽  
Wet Microalgae ◽  
Disruption Method ◽  
Chloroform Methanol

Microalgae have emerged as one of the most promising options for biodiesel production over the past few decades. Lipid extraction from microalgae for biodiesel production as a bottleneck of biodiesel production technology was the main purpose of this study. In this study different methods of the cell wall disruption were compared. Then, two methods of ultrasound and bead mill were used as methods of the cell wall disruption. The maximum lipid extracted by ultrasound was 17.10% and by bead mill was 15.16% (based on microalgae biomass dry weight). After the cell wall disruption of microalgae, for lipid extraction, chloroform-methanol solvent combination was used as a high extraction method and hexane-ethanol solvent combination was used as an environmentally friendly method. In this regard, the effect of solvent to biomass ratio, temperature and extraction time was investigated and the optimal results for chloroform-methanol solvent combination were 8 ml/g, 45°C and 60 minutes, respectively, and for hexane-ethanol combination were 6 ml/g, 35◦C and 73 minutes, respectively. Under these optimal conditions, the highest amount of extracted lipid from Chlorella vulgaris with a moisture content of 87.50%, and ultrasound as a cell wall disruption method were obtained 20.39% and 16.41% (based on microalgae dry weight) with a combination of chloroform-methanol solvents and hexane-ethanol respectively. Also the highest extraction rates of 17.63% and 13.85% were obtained for the combination of chloroform-methanol and hexane-ethanol solvents, respectively by bead milling as cell wall disruption method

scholarly journals Effect of different cell disruption methods on lipid yield of Schizochytrium sp.

2021 ◽  
Author(s):  
Mustafa Hac İsa ◽  
Cansu Metin ◽  
Ertan Ercan ◽  
Yunus Alparslan
Keyword(s):  
Fatty Acids ◽  
Osmotic Shock ◽  
Lipid Extraction ◽  
Extraction Methods ◽  
Cell Disruption ◽  
Health Indices ◽  
Lipid Yield ◽  
Atherogenicity Index ◽  
Total Fatty Acids ◽  
Enzyme Application

In this study, it was investigated to increase the lipid yield of the microalgae Schizochytrium sp., by applying different cell disruption methods. Therefore, acid treatment with HCl, osmotic shock, enzyme applications and ultrasonic homogenizer were tried in this algae species combined with the Bligh and Dyer and Soxhlet methods as an alternative to classical lipid extraction methods. As a result of the study, the highest lipid value (21.72 ± 0.74%) was obtained in enzyme application with Bligh and Dyer method (BDE). The cell disruption processes increased the lipid yield compared to the control groups. The highest PUFA DHA was found in the range of 4.58 ± 2.44-19.25 ± 0.09%, and the highest value was observed in the BDE group. Highest SFA was palmitic acid. Effective results were observed in the Bligh and Dyer applied groups in terms of both total lipid and total fatty acids. In cell disruption methods, particularly in enzyme and HCl extraction, good results were obtained in terms of fatty acids. The highest total fatty acids and the highest lipid content were detected in the Bligh and Dyer enzyme (BDE). Enzyme applications are also advantageous because of being environmentally friendly. Lipid health indices such as n-6/n-3, PUFA/SFA, Atherogenicity index (AI), Thrombogenicity index (TI) and hypocholesterolemic/hypercholesterolemic ratios (HH) were almost favorable. With this study, an appropriate lipid extraction methods were determined to provide an economical and environmental friendly suggestion for future studies to be used in areas such as food, feed and cosmetics.

Passive cell disruption lipid extraction methods of microalgae for biofuel production – A review

Fuel ◽  
2019 ◽  
Vol 252 ◽  
pp. 699-709 ◽  
Author(s):  
Senthil Nagappan ◽  
Saravanan Devendran ◽  
Pei-Chien Tsai ◽  
Selvapriya Dinakaran ◽  
Hans-Uwe Dahms ◽  
...  
Keyword(s):  
Lipid Extraction ◽  
Extraction Methods ◽  
Cell Disruption ◽  
Biofuel Production

scholarly journals AN INSIGHT ON ALGAL CELL DISRUPTION FOR BIODIESEL PRODUCTION

2018 ◽  
Vol 11 (2) ◽  
pp. 21 ◽  
Author(s):  
Aarthy A ◽  
Smita Kumari ◽  
Prachi Turkar ◽  
Sangeetha Subramanian
Keyword(s):  
Cell Wall ◽  
Enhanced Recovery ◽  
Algal Cell ◽  
Lipid Extraction ◽  
Algal Species ◽  
Cost Effective ◽  
Downstream Processing ◽  
Cell Disruption ◽  
Biodiesel Production ◽  
Cell Wall Disruption

 Objective: This review article deals with the effect that various cell disruption techniques have on the efficiency of lipid extraction. We have reviewed existing algal cell disruption techniques that aid the biodiesel production process.Methods: Current rise in demand for energy has led the researcher to focus on the production of sustainable fuels, among which biodiesel has received greater attention. This is due to its larger lipid content, higher growth rate, larger biomass production, and lower land use. Extraction of lipid from algae (micro and macro) for the production of biodiesel involves numerous downstream processing steps, of which cell wall disruption is a crucial step. Bead milling, high-pressure homogenization, ultra-sonication, freeze-drying, acid treatment, and enzymatic lysis are some methods of cell disruption. The cell disruption technique needs to be optimized based on the structure and biochemical composition of algae.Result: The lipid extraction efficiency varies depending on the algal species and the cell disruption technique used.Conclusion: In-depth research and development of new techniques are required to further enhance the cell disruption of the algal cell wall for the enhanced recovery of lipids. In addition, the operating costs and energy consumption should also be optimized for the cost-effective recovery.

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