Screening and target identification of bioactive compounds that modulate cell migration and autophagy

2016 ◽  
Vol 24 (15) ◽  
pp. 3283-3290 ◽  
Author(s):  
Etsu Tashiro ◽  
Masaya Imoto
Keyword(s):  
Cell Migration ◽  
Bioactive Compounds ◽  
Target Identification

scholarly journals Differential inhibition of gelatinase activity in human colon adenocarcinoma cells by Aloe vera and Aloe arborescens extracts

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Ana Lima ◽  
Paula Batista-Santos ◽  
Eduarda Veríssimo ◽  
Patrícia Rebelo ◽  
Ricardo Boavida Ferreira
Keyword(s):  
Colon Cancer ◽  
Cell Proliferation ◽  
Wound Healing ◽  
Cell Migration ◽  
Cancer Cell ◽  
Bioactive Compounds ◽  
Human Colon ◽  
Cancer Cell Proliferation ◽  
Cancer Cell Migration ◽  
Different Types

Abstract Background Aloe’s reported bioactivities (anticancer, anti-inflammatory and wound healing) suggest they might inhibit a subgroup of matrix metalloproteinases (MMPs) called gelatinases (MMP-2 and MMP-9). The goal of the present study was to compare the MMP inhibitory potential of two Aloe species, A. vera and A. arborescens. Methods Different types of extraction were tested and specific bioactive compounds were quantified. Cancer cell invasion inhibitory activities were measured in vitro using the wound healing assay in human colon cancer cells (HT29). Effects on gelatinase activities were further assessed by dye-quenched gelatin and gelatin zymography. Results Different types of extraction yielded significantly different levels of bioactivities and of bioactive compounds, which might be due to a greater amount of extractable bioactive compounds such as anthraquinones. Both A. arborescens and A. vera have potential as inhibitory agents in cancer cell proliferation via MMP-9 and MMP-2 enzymatic activity inhibition, being able to reduce colon cancer cell proliferation and migration but A. arborescens showed to be a more effective inhibitor of cancer cell migration than A. vera. Conclusion This work opens novel perspectives on the mode of action of Aloe species in cancer cell migration and may provide clues as to why there are so many conflicting results on Aloe’s activities.

Integrated profiling methods for identifying the targets of bioactive compounds: MorphoBase and ChemProteoBase

2016 ◽  
Vol 33 (5) ◽  
pp. 621-625 ◽  
Author(s):  
Makoto Muroi ◽  
Yushi Futamura ◽  
Hiroyuki Osada
Keyword(s):  
Bioactive Compounds ◽  
Target Identification

Target identification of new bioactive compounds has been achieved by both our direct and indirect approaches. Here, we highlight the utility of the latter approaches, MorphoBase and ChemProteoBase.

Target identification of bioactive compounds

2012 ◽  
Vol 20 (6) ◽  
pp. 1910-1921 ◽  
Author(s):  
Etsu Tashiro ◽  
Masaya Imoto
Keyword(s):  
Bioactive Compounds ◽  
Target Identification

scholarly journals Enzyme-Aided Extraction of Fucoidan by AMG Augments the Functionality of EPCs through Regulation of the AKT/Rheb Signaling Pathway

Marine Drugs ◽  
2019 ◽  
Vol 17 (7) ◽  
pp. 392
Author(s):  
Vinoth Kumar Rethineswaran ◽  
Yeon-Ju Kim ◽  
Woong Bi Jang ◽  
Seung Taek Ji ◽  
Songhwa Kang ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Wound Healing ◽  
Cell Migration ◽  
Progenitor Cells ◽  
Signaling Pathway ◽  
Endothelial Progenitor Cells ◽  
Bioactive Compounds ◽  
Tube Formation ◽  
Oxygen Species ◽  
Endothelial Progenitor

The purpose of the present study is to improve the endothelial progenitor cells (EPC) activation, proliferation, and angiogenesis using enzyme-aided extraction of fucoidan by amyloglucosidase (EAEF-AMG). Enzyme-aided extraction of fucoidan by AMG (EAEF-AMG) significantly increased EPC proliferation by reducing the reactive oxygen species (ROS) and decreasing apoptosis. Notably, EAEF-AMG treated EPCs repressed the colocalization of TSC2/LAMP1 and promoted perinuclear localization of mTOR/LAMP1 and mTOR/Rheb. Moreover, EAEF-AMG enhanced EPC functionalities, including tube formation, cell migration, and wound healing via regulation of AKT/Rheb signaling. Our data provided cell priming protocols to enhance therapeutic applications of EPCs using bioactive compounds for the treatment of CVD.

scholarly journals Bioactive Compounds from Posidonia oceanica (L.) Delile Impair Malignant Cell Migration through Autophagy Modulation

Marine Drugs ◽  
2018 ◽  
Vol 16 (4) ◽  
pp. 137 ◽  
Author(s):  
Manuela Leri ◽  
Matteo Ramazzotti ◽  
Marzia Vasarri ◽  
Sara Peri ◽  
Emanuela Barletta ◽  
...  
Keyword(s):  
Cell Migration ◽  
Bioactive Compounds ◽  
Malignant Cell ◽  
Posidonia Oceanica

Target identification of natural products and bioactive compounds using affinity-based probes

2016 ◽  
Vol 33 (5) ◽  
pp. 612-620 ◽  
Author(s):  
Sijun Pan ◽  
Hailong Zhang ◽  
Chenyu Wang ◽  
Samantha C. L. Yao ◽  
Shao Q. Yao
Keyword(s):  
Natural Products ◽  
Bioactive Compounds ◽  
Drug Target ◽  
Target Identification ◽  
Direct Capture

Direct capture of drug–target complexesin situby using affinity-based probes allows target identification of natural products and bioactive compounds, even if the binding is reversible with moderate affinity.

Screening and synthesis: high throughput technologies applied to parasitology

Parasitology ◽  
2004 ◽  
Vol 128 (S1) ◽  
pp. S71-S79 ◽  
Author(s):  
R. E. MORGAN ◽  
N. J. WESTWOOD
Keyword(s):  
Bioactive Compounds ◽  
High Throughput ◽  
High Throughput Screening ◽  
Target Identification ◽  
Protein Target ◽  
Genome Projects

High throughput technologies continue to develop in response to the challenges set by the genome projects. This article discusses how the techniques of both high throughput screening (HTS) and synthesis can influence research in parasitology. Examples of the use of targeted and phenotype-based HTS using unbiased compound collections are provided. The important issue of identifying the protein target(s) of bioactive compounds is discussed from the synthetic chemist's perspective. This article concludes by reviewing recent examples of successful target identification studies in parasitology.

Neutrophil migration through in vitro interstitial matrix

1992 ◽  
Vol 50 (1) ◽  
pp. 894-895
Author(s):  
J. Roemer ◽  
S.R. Simon
Keyword(s):  
Extracellular Matrix ◽  
Smooth Muscle ◽  
Cell Migration ◽  
Smooth Muscle Cells ◽  
Inflammatory Cell ◽  
Neutrophil Migration ◽  
Culture Conditions ◽  
Aortic Smooth Muscle ◽  
Specific Culture

We are developing an in vitro interstitial extracellular matrix (ECM) system for study of inflammatory cell migration. Falcon brand Cyclopore membrane inserts of various pore sizes are used as a support substrate for production of ECM by R22 rat aortic smooth muscle cells. Under specific culture conditions these cells produce a highly insoluble matrix consisting of typical interstitial ECM components, i.e.: types I and III collagen, elastin, proteoglycans and fibronectin.

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