Tartrate-resistant acid phosphatase isoform 5b (TRACP 5b) as a serum maker for cancer with bone metastasis

Serum tartrate-resistant acid phosphatase (TRACP) 5b was investigated for use as a marker for diagnosis of giant cell tumor (GCT) of bone and for detection of its recurrence.Four patients with GCT of bone who were initially referred to our hospital were classified as a primary group. Three patients who had local recurrence following curettage were classified as a local recurrence group. Five with no recurrence were classified as a no-recurrence group. Eighteen patients with primary and metastatic malignant bone tumors were also enrolled in the study as a control group. Serum TRACP 5b was measured before the biopsy in all patients and was measured periodically after the operation in patients with GCT of bone. Studentt-tests were used for statistical analyses.TRACP 5b was greater than 1500 Um/dL in all primary group patients. Mean TRACP 5b values decreased gradually with post-operative time, showing lower values until local recurrence. The mean value of TRACP 5b of the local recurrence group (753 ± 68.7 mU/dL) was significantly higher than that of the no-recurrence group (340.6 ± 78.3 mU/dL). The mean value of TRACP 5b of the control group (466.9 ± 130.3 mU/dL) was much lower than that of the primary group and markedly lower than that of the local recurrence group. However, no significant difference was found between the no-recurrence group and the control group.Serum TRACP 5b is a useful and convenient marker for diagnosing GCT of bone and for predicting its recurrence.

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IL-12 Inhibits Lipopolysaccharide Stimulated Osteoclastogenesis in Mice

Journal of Immunology Research ◽

10.1155/2015/214878 ◽

2015 ◽

Vol 2015 ◽

pp. 1-8 ◽

Cited By ~ 6

Author(s):

Masako Yoshimatsu ◽

Hideki Kitaura ◽

Yuji Fujimura ◽

Haruka Kohara ◽

Yukiko Morita ◽

...

Keyword(s):

Acid Phosphatase ◽

Bone Resorption ◽

Host Defense ◽

Inflammatory Cytokine ◽

Mrna Level ◽

Critical Role ◽

Tartrate Resistant Acid Phosphatase ◽

Bone Resorption Marker ◽

Mrna Levels ◽

Tracp 5B

Lipopolysaccharide (LPS) is related to osteoclastogenesis in osteolytic diseases. Interleukin- (IL-) 12 is an inflammatory cytokine that plays a critical role in host defense. In this study, we investigated the effects of IL-12 on LPS-induced osteoclastogenesis. LPS was administered with or without IL-12 into the supracalvariae of mice, and alterations in the calvarial suture were evaluated histochemically. The number of osteoclasts in the calvarial suture and the mRNA level of tartrate-resistant acid phosphatase (TRAP), an osteoclast marker, were lower in mice administered LPS with IL-12 than in mice administered LPS alone. The serum level of tartrate-resistant acid phosphatase 5b (TRACP 5b), a bone resorption marker, was also lower in mice administered LPS with IL-12 than in mice administered LPS alone. These results revealed that IL-12 might inhibit LPS-induced osteoclastogenesis and bone resorption. In TdT-mediated dUTP-biotin nick end-labeling (TUNEL) assays, apoptotic changes in cells were recognized in the calvarial suture in mice administered LPS with IL-12. Furthermore, the mRNA levels of both Fas and FasL were increased in mice administered LPS with IL-12. Taken together, the findings demonstrate that LPS-induced osteoclastogenesis is inhibited by IL-12 and that this might arise through apoptotic changes in osteoclastogenesis-related cells induced by Fas/FasL interactions.

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Tartrate-Resistant Acid Phosphatase Isoform 5B (TRACP 5B) As Serum Marker of Bone Metastases From Non-Small Cell Lung Cancer (NSCLC) and Breast Cancer. Preliminary Results

Annals of Oncology ◽

10.1093/annonc/mdv043.17 ◽

2015 ◽

Vol 26 ◽

pp. i1

Author(s):

F. Lumachi ◽

A. Del Conte ◽

F. Mazza ◽

S.M.M. Basso ◽

G.B. Chiara

Keyword(s):

Breast Cancer ◽

Lung Cancer ◽

Acid Phosphatase ◽

Small Cell Lung Cancer ◽

Bone Metastases ◽

Serum Marker ◽

Small Cell ◽

Tartrate Resistant Acid Phosphatase ◽

Small Cell Lung ◽

Tracp 5B

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Tartrate-Resistant Acid Phosphatase 5b in Young Patients With Sickle Cell Disease and Trait Siblings

Clinical and Applied Thrombosis/Hemostasis ◽

10.1177/1076029615594001 ◽

2016 ◽

Vol 23 (1) ◽

pp. 64-71 ◽

Cited By ~ 2

Author(s):

Galila Mohamed Mokhtar ◽

Azza Abdel Gawad Tantawy ◽

Ahmed Al-Saeed Hamed ◽

Amira Abdel Moneam Adly ◽

Eman Abdel Rahman Ismail ◽

...

Keyword(s):

Acid Phosphatase ◽

Sickle Cell Disease ◽

Sickle Cell ◽

Young Patients ◽

Tartrate Resistant Acid Phosphatase ◽

Cell Disease ◽

Dxa Scan ◽

Significant Difference ◽

Tracp 5B ◽

Bone Complications

Bone involvement is a frequent cause of acute morbidity in sickle cell disease (SCD). Tartrate-resistant acid phosphatase 5b (TRACP 5b), a bone resorption marker, is produced specifically by activated osteoclasts. We assessed bone mineral density (BMD) in 30 young patients with SCD and 17 asymptomatic patients with sickle cell trait (SCT) compared with 32 healthy controls and determined TRACP 5b levels in relation to vascular complications. Serum ferritin, alkaline phosphatase (ALP), and TRACP 5b were measured. Echocardiography was performed with assessment of BMD using dual energy X-ray absorptiometry (DXA). The BMD was decreased in patients with SCD compared with SCT and controls ( P = .005), with no significant difference between the latter 2 groups. Patients with SCD had higher incidence of bone complications than SCT group and controls ( P = .03). The SCD group with abnormal DXA scan had higher ferritin and ALP than normal BMD. Serum TRACP 5b was significantly higher in patients with SCD than SCT and controls ( P = .003). The TRACP 5b levels were associated with severe vaso-occlusive crisis ( P = .022). Patients treated with hydroxyurea and those on chelation therapy had lower TRACP 5b levels than untreated patients. The TRACP 5b level was positively correlated with lactate dehydrogenase, while there was no relation with ferritin, ALP, or BMD. We suggest that bone complications frequently occur in SCD as reflected by low BMD and high ALP and TRACP 5b. Hemolysis and iron overload may be involved in the occurrence of these complications. The lack of correlation between abnormal DXA scan and high TRACP 5b suggests that bone disease in SCD is multifactorial.

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Osteoclast-Derived Serum Tartrate-Resistant Acid Phosphatase 5b in Albers-Schönberg Disease (Type II Autosomal Dominant Osteopetrosis)

Clinical Chemistry ◽

10.1373/clinchem.2003.029355 ◽

2004 ◽

Vol 50 (5) ◽

pp. 883-890 ◽

Cited By ~ 79

Author(s):

Sari L Alatalo ◽

Kaisa K Ivaska ◽

Steven G Waguespack ◽

Michael J Econs ◽

H Kalervo Väänänen ◽

...

Keyword(s):

Acid Phosphatase ◽

Autosomal Dominant ◽

Gene Mutations ◽

Serum Markers ◽

High Serum ◽

Tartrate Resistant Acid Phosphatase ◽

Type Ii ◽

Gene Carriers ◽

Tracp 5B ◽

Autosomal Dominant Osteopetrosis

Abstract Background: Albers-Schönberg disease, or autosomal dominant osteopetrosis type II (ADO2), is caused by ineffective osteoclastic bone resorption resulting from mutations in the chloride channel 7 (ClCN7) gene. Individuals with ADO2 have increased numbers of large ineffective osteoclasts in addition to increased serum total tartrate-resistant acid phosphatase (TRACP) activity. Methods: We investigated the serum activity of the osteoclast-derived 5b isoform of TRACP (TRACP 5b) and concentrations of the bone formation marker osteocalcin in clinically affected individuals, unaffected gene carriers, and healthy controls from 10 ADO2 families with known ClCN7 gene mutations. Bone fracture prevalence was studied in association with the serum markers. Results: Similar to total TRACP, TRACP 5b was significantly increased in clinically affected individuals compared with age-matched controls. TRACP 5b correlated significantly with total TRACP (r = 0.833; P <0.001), suggesting that most of the TRACP in the serum of ADO2 patients is osteoclast-derived TRACP 5b. Osteocalcin was significantly increased in affected adults and slightly decreased in affected children. TRACP 5b and total TRACP were significantly increased in clinically affected individuals with severe fractures (P <0.05). Conclusions: The results indicate that in ADO2, serum TRACP 5b reflects the number of osteoclasts and that the extremely high serum TRACP 5b activity is a specific indicator of the disease. Similar to total TRACP, TRACP 5b appears to be a potentially useful marker to stratify individuals with ClCN7 gene mutations into clinically affected and unaffected gene carriers. It may also have a prognostic value in the prediction of fractures in patients with a ClCN7 gene mutation.

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Serum tartrate-resistant acid phosphatase 5b (TRACP 5b) as a marker of skeletal changes in prostate cancer

Acta Oncologica ◽

10.1080/02841860500327586 ◽

2005 ◽

Vol 44 (7) ◽

pp. 742-747 ◽

Cited By ~ 10

Author(s):

Eeva Salminen ◽

M Ala-Houhala ◽

J Korpela ◽

M Varpula ◽

S. L. Tiitinen ◽

...

Keyword(s):

Prostate Cancer ◽

Acid Phosphatase ◽

Tartrate Resistant Acid Phosphatase ◽

Skeletal Changes ◽

Tracp 5B

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Serum tartrate-resistant acid phosphatase 5b (TRACP5b) activity as a biomarker for bone metastasis in non-small cell lung cancer patients

Clinica Chimica Acta ◽

10.1016/j.cca.2010.09.038 ◽

2011 ◽

Vol 412 (1-2) ◽

pp. 181-185 ◽

Cited By ~ 22

Author(s):

Nai-Shun Yao ◽

Yi-Ying Wu ◽

Anthony J. Janckila ◽

Chih-Hung Ku ◽

An-Tai Hsieh ◽

...

Keyword(s):

Lung Cancer ◽

Acid Phosphatase ◽

Bone Metastasis ◽

Small Cell Lung Cancer ◽

Cancer Patients ◽

Cell Lung Cancer ◽

Small Cell ◽

Tartrate Resistant Acid Phosphatase ◽

Small Cell Lung ◽

Lung Cancer Patients

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Effects of cadmium on bone microstructure and serum tartrate-resistant acid phosphatase 5b in male rats

Experimental Biology and Medicine ◽

10.1258/ebm.2011.011104 ◽

2011 ◽

Vol 236 (11) ◽

pp. 1298-1305 ◽

Cited By ~ 11

Author(s):

Xiao Chen ◽

Guoying Zhu ◽

Chunlin Shao ◽

Taiyi Jin ◽

Mingguang Tan ◽

...

Keyword(s):

Acid Phosphatase ◽

Bone Mineral ◽

Bone Microstructure ◽

Male Rats ◽

Control Group ◽

Tartrate Resistant Acid Phosphatase ◽

Sprague Dawley ◽

Mineral Density ◽

Microstructure Parameters ◽

Tracp 5B

This study investigated the effects of cadmium on bone microstructure and serum tartrate-resistant acid phosphatase 5b (Tracp 5b) in male rats. Sprague-Dawley male rats were divided into three groups that were given CdCl2 by subcutaneous injection at doses of 0, 0.1 and 0.5 mg/kg body weight (bw) for 12 weeks, respectively. Before killing at the 12th week, microcomputed tomography scanning was performed on the proximal tibia, and urine samples were collected from all of the rats. All rats were then killed, and their blood was collected for biomarkers assay. Bone tissues were dissected for mineral density determinations and histology. The concentration of cadmium in the blood, urine and bone of rats treated with cadmium were significantly higher than in the control group. The bone mineral density, bone mineral concentrations and bone microstructure index of rats treated with cadmium at 0.5 mg/kg bw were clearly lower than in the control rats. Histological investigation also revealed damage to the bone microstructure caused by cadmium. Tracp 5b concentrations in rats treated with cadmium were dose dependently higher than the control. The concentration of cadmium in blood, urine and bone was significantly correlated with Tracp 5b and bone microstructure parameters. Cadmium was shown to induce bone microstructure damage, especially to trabecular bone. The elevated concentrations of serum Tracp 5b suggest that bone resorption mediated via osteoclasts is an important mechanism for the toxic effects of cadmium on bone.

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