Array-based sensing of amyloidogenic proteins and discrimination of cancer by using different oxidants doped carbon nanodots as fluorescent probes

2022 ◽  
Vol 430 ◽  
pp. 132696
Author(s):  
Qin-Ying Li ◽  
Liyun Ma ◽  
Li Li ◽  
Shilin Wang ◽  
Xi Li ◽  
...  
Keyword(s):  
Fluorescent Probes ◽  
Carbon Nanodots ◽  
Amyloidogenic Proteins

scholarly journals Ultraviolet Carbon Nanodots Providing a Dual-Mode Spectral Matching Platform for Synergistic Enhancement of the Fluorescent Sensing

Molecules ◽  
2020 ◽  
Vol 25 (11) ◽  
pp. 2679
Author(s):  
Liman Sai ◽  
Shuping Jiao ◽  
Jianwen Yang
Keyword(s):  
Fluorescent Probes ◽  
High Sensitivity ◽  
Inner Filter Effect ◽  
Carbon Nanodots ◽  
Spectral Matching ◽  
Fluorescent Sensing ◽  
Chromium Vi ◽  
Band Emission ◽  
Synergistic Enhancement ◽  
Emissive Property

The sensing of chromium(VI) (Cr(VI)) is highly desired, due to its toxic and carcinogenic effects upon human health. Fluorescent probes, especially carbon nanodots (CNDs), have been widely used for Cr(VI) sensing via the inner filter effect (IFE). However, improving the sensitivity of these probes remains a difficult issue. In this work, CNDs derived from β-Lactoglobulin were applied as an ultrasensitive fluorescent probe for Cr(VI). With 260 nm excitation, the CNDs showed multi-band emission, including an ultraviolet 360 nm peak. The spectral matching of the CNDs with Cr(VI) led to synergistic suppression of both the excitation and emission light in the fluorescent sensing. As a consequence, the CNDs showed high sensitivity toward Cr(VI), the detection limit reaching as low as 20 nM. Moreover, taking advantage of the multi-emissive property of the CNDs, the synergistic effect was proven in an IFE-based sensing system, which might be extended to the design of other kinds of fluorescent probes.

Carbon nanodots prepared for cellular imaging and turn-on detection of glutathione

2016 ◽  
Vol 8 (23) ◽  
pp. 4736-4743 ◽  
Author(s):  
Depeng Kong ◽  
Fanyong Yan ◽  
Yunmei Luo ◽  
Yinyin Wang ◽  
Li Chen ◽  
...  
Keyword(s):  
Hydrothermal Method ◽  
Fluorescent Probes ◽  
Cellular Imaging ◽  
Carbon Nanodots ◽  
Intracellular Imaging ◽  
Turn On ◽  
Highly Sensitive ◽  
One Step

Carbon nanodots as fluorescent probes were synthesized through a one-step hydrothermal method. The carbon nanodots can be used not only for multicolor intracellular imaging but also for highly sensitive and selective turn-on detection of glutathione.

scholarly journals Multicolor carbon nanodots from food waste and their heavy metal ion detection application

RSC Advances ◽  
2018 ◽  
Vol 8 (42) ◽  
pp. 23657-23662 ◽  
Author(s):  
Ying Zhou ◽  
Yao Liu ◽  
Yeqing Li ◽  
Ziying He ◽  
Quan Xu ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Food Waste ◽  
Fluorescent Probes ◽  
Carbon Dots ◽  
Metal Ion ◽  
Heavy Metal Ion ◽  
Carbon Nanodots ◽  
Iron Ions ◽  
Carbonaceous Materials ◽  
Metal Ion Detection

Multicolor carbon dots produced from green carbonaceous materials by disposing of food waste through the HTC process could be used as fluorescent probes to detect iron ions.

Convenient synthesis of carbon nanodots for detecting Cr(vi) and ascorbic acid by fluorimetry

2020 ◽  
Vol 44 (47) ◽  
pp. 20806-20811
Author(s):  
Dongxiu Wang ◽  
Lin Zhang ◽  
Pengxia Li ◽  
Junfen Li ◽  
Chuan Dong
Keyword(s):  
Ascorbic Acid ◽  
Fluorescent Probes ◽  
Carbon Nanodots ◽  
Sophora Flavescens ◽  
Real Samples ◽  
Convenient Synthesis ◽  
Sophora Flavescens Ait

Carbon nanodots (CDs) were simply synthesized from Sophora flavescens Ait. “On–off–on” fluorescent probes for the sensitive and selective detections of Cr(iv) and ascorbic acid (AA) were founded and well applied in real samples.

Label-free iodide detection using functionalized carbon nanodots as fluorescent probes

2020 ◽  
Vol 412 (12) ◽  
pp. 2893-2901 ◽  
Author(s):  
Jingqi Chen ◽  
Xiaowei Liu ◽  
Xialing Hou ◽  
Yingying Chen ◽  
Feifei Xing ◽  
...  
Keyword(s):  
Fluorescent Probes ◽  
Carbon Nanodots ◽  
Label Free

Photosensitizer-Conjugated Ultrasmall Carbon Nanodots as Multifunctional Fluorescent Probes for Bioimaging

2016 ◽  
Vol 120 (29) ◽  
pp. 15867-15874 ◽  
Author(s):  
Alexandre Loukanov ◽  
Ryota Sekiya ◽  
Midori Yoshikawa ◽  
Naritaka Kobayashi ◽  
Yuji Moriyasu ◽  
...  
Keyword(s):  
Fluorescent Probes ◽  
Carbon Nanodots

scholarly journals Protein-derived carbon nanodots with an ethylenediamine-modulated structure as sensitive fluorescent probes for Cu2+detection

RSC Advances ◽  
2017 ◽  
Vol 7 (27) ◽  
pp. 16608-16615 ◽  
Author(s):  
Liman Sai ◽  
Jun Chen ◽  
Quanhong Chang ◽  
Wangzhou Shi ◽  
Qi Chen ◽  
...  
Keyword(s):  
Fluorescent Probes ◽  
Carbon Nanodots ◽  
Modulated Structure ◽  
Sensing Applications ◽  
Analytical Capability ◽  
Fluorescent Carbon Nanodots ◽  
Fluorescent Carbon

The increasing use of fluorescent carbon nanodots (CNDs) demonstrates their advantages for sensing applications; these include superior photostability, absence of toxicity, and rapid analytical capability.

Confocal microscopy of the cytoskeleton in living plant cells following microinjection of fluorescent probes

1993 ◽  
Vol 51 ◽  
pp. 130-131
Author(s):  
Ann Cleary
Keyword(s):  
Cell Division ◽  
Fluorescent Probes ◽  
Actin Filaments ◽  
Intracellular Transport ◽  
Cell Structure ◽  
Plant Cells ◽  
Cell Plate ◽  
Cell Cortex ◽  
Living Plant ◽  
Rhodamine Phalloidin

Microinjection of fluorescent probes into living plant cells reveals new aspects of cell structure and function. Microtubules and actin filaments are dynamic components of the cytoskeleton and are involved in cell growth, division and intracellular transport. To date, cytoskeletal probes used in microinjection studies have included rhodamine-phalloidin for labelling actin filaments and fluorescently labelled animal tubulin for incorporation into microtubules. From a recent study of Tradescantia stamen hair cells it appears that actin may have a role in defining the plane of cell division. Unlike microtubules, actin is present in the cell cortex and delimits the division site throughout mitosis. Herein, I shall describe actin, its arrangement and putative role in cell plate placement, in another material, living cells of Tradescantia leaf epidermis.The epidermis is peeled from the abaxial surface of young leaves usually without disruption to cytoplasmic streaming or cell division. The peel is stuck to the base of a well slide using 0.1% polyethylenimine and bathed in a solution of 1% mannitol +/− 1 mM probenecid.

Live Confocal Analysis of Fertilization and Early Development

2001 ◽  
Vol 7 (S2) ◽  
pp. 1012-1013
Author(s):  
Uyen Tram ◽  
William Sullivan
Keyword(s):  
Drosophila Melanogaster ◽  
Embryonic Development ◽  
Real Time ◽  
Early Development ◽  
Fluorescent Probes ◽  
Primary Defect ◽  
Fluorescent Analysis ◽  
Dynamic Event ◽  
Enormous Amount ◽  
Fluorescent Studies

Embryonic development is a dynamic event and is best studied in live animals in real time. Much of our knowledge of the early events of embryogenesis, however, comes from immunofluourescent analysis of fixed embryos. While these studies provide an enormous amount of information about the organization of different structures during development, they can give only a static glimpse of a very dynamic event. More recently real-time fluorescent studies of living embryos have become much more routine and have given new insights to how different structures and organelles (chromosomes, centrosomes, cytoskeleton, etc.) are coordinately regulated. This is in large part due to the development of commercially available fluorescent probes, GFP technology, and newly developed sensitive fluorescent microscopes. For example, live confocal fluorescent analysis proved essential in determining the primary defect in mutations that disrupt early nuclear divisions in Drosophila melanogaster. For organisms in which GPF transgenics is not available, fluorescent probes that label DNA, microtubules, and actin are available for microinjection.

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