scholarly journals Rapid selection of HIV envelopes that bind to neutralizing antibody B cell lineage members with functional improbable mutations

Cell Reports ◽  
2021 ◽  
Vol 36 (7) ◽  
pp. 109561
Author(s):  
Olivia Swanson ◽  
Brianna Rhodes ◽  
Avivah Wang ◽  
Shi-Mao Xia ◽  
Robert Parks ◽  
...  
Keyword(s):  
B Cell ◽  
Cell Lineage ◽  
Neutralizing Antibody ◽  
Selection Of

scholarly journals Rapid selection of HIV envelopes that bind to neutralizing antibody B cell lineage members with functional improbable mutations

2021 ◽  
Author(s):  
Olivia Swanson ◽  
Brianna Rhodes ◽  
Avivah Wang ◽  
Shi-Mao Xia ◽  
Robert Parks ◽  
...  
Keyword(s):  
B Cell ◽  
Neutralizing Antibodies ◽  
Cell Lineage ◽  
Neutralizing Antibody ◽  
Developmental Pathways ◽  
Broadly Neutralizing Antibodies ◽  
Minimal Subset ◽  
Functional Features ◽  
Selection Of

SummaryElicitation of broadly neutralizing antibodies (bnAbs) by an HIV vaccine will involve priming the immune system to activate antibody precursors, followed by boosting immunizations to select for antibodies with functional features required for neutralization breadth. The higher the number of mutations necessary for function, the more convoluted are the antibody developmental pathways. HIV bnAbs acquire a large number of somatic mutations, but not all mutations are functionally important. Here we identified a minimal subset of mutations sufficient for the function of the V3-glycan bnAb DH270.6. Using antibody library screening, candidate envelope immunogens that interacted with DH270.6-like antibodies containing this set of key mutations were identified and selected in vitro. Our results demonstrate that less complex B cell evolutionary pathways than those naturally observed exist for the induction of HIV bnAbs by vaccination, and establish rational approaches to identify boosting sequential envelope candidate immunogens.

scholarly journals ­­­­Rapid Selection of HIV Envelopes that Bind to Neutralizing Antibody B Cell Lineage Members with Functional Improbable Mutations

2021 ◽  
Author(s):  
Olivia Swanson ◽  
Brianna Rhodes ◽  
Avivah Wang ◽  
Shi-Mao Xia ◽  
Melissa Cooper ◽  
...  
Keyword(s):  
B Cell ◽  
Cell Lineage ◽  
Neutralizing Antibody ◽  
Selection Of

scholarly journals Rearrangement and selection of VH11 in the Ly-1 B cell lineage.

1990 ◽  
Vol 172 (1) ◽  
pp. 371-374 ◽  
Author(s):  
C E Carmack ◽  
S A Shinton ◽  
K Hayakawa ◽  
R R Hardy
Keyword(s):  
Polymerase Chain Reaction ◽  
B Cells ◽  
B Cell ◽  
Peritoneal Cavity ◽  
Cell Lineage ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Vh Genes ◽  
Self Antigen ◽  
Selection Of

One of the predominant VH genes utilized to encode the anti-BrMRBC specificity is a member of the small VH11 family rearranged to JH1. Using the polymerase chain reaction (PCR) we have determined that the frequency of B cells with a VH11 rearrangement is 10-20 times higher in Ly-1 B than in Ly-1- "conventional" B cells regardless of location (spleen or peritoneal cavity). Conventional B cells rearrange this gene at comparable levels in pre-B cells and in mature B cells utilizing all JH gene segments. In contrast, the increased levels of VH11 rearrangement in Ly-1 B are restricted to JH1 (and some JH2) and therefore appear to be the result of selection. Furthermore, most peritoneal Ly-1 B cells with VH11 rearrangements fall in a fraction stained by anti-BrMRBC antibody, likely bearing multivalent natural (likely self) antigen constitutively bound to their surface Ig receptors. Thus, we suggest that autoantigens are largely responsible for the accumulation of autoantibody specificities in the Ly-1 B cell lineage with time, whereas they do not exert this effect in the conventional B cells.

scholarly journals Targeted selection of HIV-specific antibody mutations by engineering B cell maturation

Science ◽  
2019 ◽  
Vol 366 (6470) ◽  
pp. eaay7199 ◽  
Author(s):  
Kevin O. Saunders ◽  
Kevin Wiehe ◽  
Ming Tian ◽  
Priyamvada Acharya ◽  
Todd Bradley ◽  
...  
Keyword(s):  
B Cell ◽  
Specific Antibody ◽  
Cell Maturation ◽  
B Cell Maturation ◽  
Selection Of

Selection of the expressed B cell repertoire by infusion of normal immunoglobulin G in a patient with autoimmune thyroiditis

1993 ◽  
Vol 23 (11) ◽  
pp. 2945-2950 ◽  
Author(s):  
Gilles Dietrich ◽  
Francisco J. Varela ◽  
Vincent Hurez ◽  
Majida Bouanani ◽  
Michel D. Kazatchkine
Keyword(s):  
B Cell ◽  
Immunoglobulin G ◽  
Autoimmune Thyroiditis ◽  
Cell Repertoire ◽  
B Cell Repertoire ◽  
Selection Of

scholarly journals EBF1 is expressed in pericytes and contributes to pericyte cell commitment

2021 ◽  
Author(s):  
Francesca Pagani ◽  
Elisa Tratta ◽  
Patrizia Dell’Era ◽  
Manuela Cominelli ◽  
Pietro Luigi Poliani
Keyword(s):  
Stem Cells ◽  
Endothelial Cells ◽  
Mesenchymal Stem Cells ◽  
B Cell ◽  
Cell Fate ◽  
Early Stage ◽  
Cell Lineage ◽  
Cell Maturation ◽  
Cell Commitment

AbstractEarly B-cell factor-1 (EBF1) is a transcription factor with an important role in cell lineage specification and commitment during the early stage of cell maturation. Originally described during B-cell maturation, EBF1 was subsequently identified as a crucial molecule for proper cell fate commitment of mesenchymal stem cells into adipocytes, osteoblasts and muscle cells. In vessels, EBF1 expression and function have never been documented. Our data indicate that EBF1 is highly expressed in peri-endothelial cells in both tumor vessels and in physiological conditions. Immunohistochemistry, quantitative reverse transcription polymerase chain reaction (RT-qPCR) and fluorescence-activated cell sorting (FACS) analysis suggest that EBF1-expressing peri-endothelial cells represent bona fide pericytes and selectively express well-recognized markers employed in the identification of the pericyte phenotype (SMA, PDGFRβ, CD146, NG2). This observation was also confirmed in vitro in human placenta-derived pericytes and in human brain vascular pericytes (HBVP). Of note, in accord with the key role of EBF1 in the cell lineage commitment of mesenchymal stem cells, EBF1-silenced HBVP cells showed a significant reduction in PDGFRβ and CD146, but not CD90, a marker mostly associated with a prominent mesenchymal phenotype. Moreover, the expression levels of VEGF, angiopoietin-1, NG2 and TGF-β, cytokines produced by pericytes during angiogenesis and linked to their differentiation and activation, were also significantly reduced. Overall, the data suggest a functional role of EBF1 in the cell fate commitment toward the pericyte phenotype.

scholarly journals Siglecg Limits the Size of B1a B Cell Lineage by Down-Regulating NFκB Activation

PLoS ONE ◽  
2007 ◽  
Vol 2 (10) ◽  
pp. e997 ◽  
Author(s):  
Cheng Ding ◽  
Yan Liu ◽  
Yin Wang ◽  
Bae Keun Park ◽  
Cun-Yu Wang ◽  
...  
Keyword(s):  
B Cell ◽  
Cell Lineage
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