scholarly journals Exposure to elevated ozone levels differentially affects the antioxidant capacity and the redox homeostasis of two subtropical Phaseolus vulgaris L. varieties

Chemosphere ◽  
2013 ◽  
Vol 93 (2) ◽  
pp. 320-330 ◽  
Author(s):  
Fernanda Freitas Caregnato ◽  
Rafael Calixto Bortolin ◽  
Armando Molina Divan Junior ◽  
José Cláudio Fonseca Moreira
2020 ◽  
Vol 4 (Supplement_2) ◽  
pp. 24-24
Author(s):  
David Fonseca Hernandez ◽  
Ignacio Orozco-Avila ◽  
Eugenia Lugo-Cervantes ◽  
Luis Mojica

Abstract Objectives The objective of this work was to evaluate the potential of common bean phenolic extract to exert anti-aging and antioxidant effect by inhibiting the collagenase, elastase, tyrosinase enzymes and free radicals. Methods 18 varieties of common bean (Phaseolus vulgaris L.) from Chiapas, Mexico, were analyzed for total phenolic content (TPC) and total anthocyanin content (ACN). Supercritical fluid (SCF) and leaching extractions were used for phenolic compounds extraction. Antioxidant capacity was evaluated using DPPH and ABTS scavenging assay. The inhibitory potential of the extract was evaluated for tyrosinase from mushroom, collagenase type-1 from Clostridium histolycum and elastase from porcine pancreas enzymes. Results The TPC ranged from 3.8–34.33 mg GAE/g coat and ACN ranged from 0.04–9.41 mg C3GE/g coat among the 18 common bean varieties (P < 0.05). The cultivar selected for this study was black bean with a TPC of 27.45 ± 0.7 mg GAE/g coat and ACN of 5.3 ± 0.1 mg C3GE/g coat. The best extraction conditions for the obtention of phenolic compounds and anthocyanins were SCF water-ethanol 50% as cosolvent, obtaining 66.60 ± 7.4 mg GAE/g coat (TPC) and 7.3 ± 0.6 mg C3GE/g coat (ACN). TPC and ACN content between each extraction process were statistically different (P < 0.05). For DPPH scavenging assay the IC50 for the black bean extract was 0.32 ± 0.01 mg GAE/g coat, and 0.40 ± 0.03 mg GAE/g coat for ABTS assay. Finally, the IC50 for the enzymatic inhibition assays of tyrosinase, collagenase and elastase were 10.44 ± 1.32, 8.33 ± 0.65 and 0.11 ± 0.02 mg GAE/g coat, respectively. Conclusions Black bean (Phaseolus vulgaris L.) extract presents high antioxidant capacity and inhibitory potential for tyrosinase and metalloproteinases such as collagenase and elastase. Black bean phenolic extracts could be used in cosmeceutical products related to preventing oxidative stress and aging. Funding Sources Author David Fonseca Hernández was supported by a scholarship from Consejo Nacional de Ciencia y Tecnología CONACyT-México, number 901,000. CONACYT-FORDECYT GRANT.


2015 ◽  
Vol 6 (5) ◽  
pp. 1470-1479 ◽  
Author(s):  
Diego A. Luna-Vital ◽  
Elvira González de Mejía ◽  
Sandra Mendoza ◽  
Guadalupe Loarca-Piña

Pure peptides present in common-bean non-digestible fraction inhibited ACE by interacting with its catalytic cavity, and presented antioxidant activities.


Biotecnia ◽  
2019 ◽  
Vol 22 (1) ◽  
pp. 117-125
Author(s):  
Liliana Maribel Pérez-Perez ◽  
Carmen Lizette Del Toro Sánchez ◽  
Esteban Sánchez Chavez ◽  
Ricardo Iván González Vega ◽  
Aline Reyes Díaz ◽  
...  

El frijol tiene alto contenido fenólico y actividad antioxidante, por ello es importante conocer cuánto se libera de estos compuestos de la matriz alimentaria y es aprovechado en el organismo. Por lo tanto, el objetivo de este trabajo fue determinar la bioaccesibilidad de compuestos antioxidantes de diferentes variedades de frijol (Phaseolus vulgaris L.) mediante un sistema gastrointestinal in vitro. Se realizó la cuantificación de compuestos fenólicos, flavonoides y capacidad antioxidante (DPPH, ABTS y FRAP) de 6 cultivares: Negro, Flor de Mayo, Patol, Pinto Saltillo, Teapa y Peruano, antes y después del cocimiento. Adicionalmente, se evaluó la digestión gastrointetinal in vitro en frijol cocido. Los resultados obtenidos de las variedades de frijol Teapa y Peruano, presentaron mayor rendimiento de extracto crudo, y un incremento del contenido fenólico. El frijol cocido, mostró un incremento en el contenido de flavonoides en las variedades Teapa, Peruano y Pinto Saltillo, así como mayor capacidad antioxidante, en las variedades Negro, Flor de mayo, Teapa y Peruano. La concentración inhibitoria media (IC50) la alcanzaron Teapa, Pinto Saltillo y Flor de Mayo (106.45, 154.72 y 127.16 μg/mL respectivamente). La digestión gastrointestinal in vitro dio como resultado mayor bioaccesibilidad para compuestos fenólicos, resaltando las variedades Teapa, Pinto Saltillo y Flor de mayo.ABSTRACTBeans have high phenolic content and antioxidant activity, so it is important to know how much of these compounds is released from the food matrix and is used in the body. Therefore, the objective of this work was to determine the antioxidant compounds bioavailability on different bean varieties (Phaseolus vulgaris L.) through an in vitro gastrointestinal system. Quantification of phenolic compounds, flavonoids and antioxidant capacity (DPPH, ABTS and FRAP) of 6 cultivars (Black, Flor de Mayo, Patol, Pinto Saltillo, Teapa and Peruvian) was carried out, before and after cooking. Additionally, in vitro gastrointetinal digestion in cooked beans was evaluated. The results obtained from the Teapa and Peruvian bean varieties showed a higher yield of crude extract and an increase in phenolic content. Cooked beans showed an increase in flavonoid content in the Teapa, Peruvian and Pinto Saltillo varieties, as well as greater antioxidant capacity, in the Negro, Flor de Mayo, Teapa and Peruvian varieties. The mean inhibitory concentration (IC50) was reached by Teapa, Pinto Saltillo and Flor de Mayo (106.45, 154.72 and 127.16 μg/mL respectively). The gastrointestinal digestion in vitro resulted in greater bioavailability for phenolic compounds, highlighting the Teapa, Pinto Saltillo and Flor de Mayo varieties.


Author(s):  
Gabriela Barbosa Rossi ◽  
Siluana Katia Tischer Seraglio ◽  
Tuany Camila Honaiser ◽  
Isabela Maia Toaldo ◽  
Ana Carolina de Oliveira Costa ◽  
...  

2001 ◽  
Vol 71 (3) ◽  
pp. 549-557
Author(s):  
Ocsana Opris ◽  
Ildiko Lung ◽  
Maria L. Soran ◽  
Lucian Copolovici ◽  
Dana M. Copolovici ◽  
...  

In the present work, the effects of acetaminophen (paracetamol) on Phaseolus vulgaris L. and Triticum aestivum L. plants, two important vegetables in Romania, were studied. The treatment consisted of sowing the selected plant seed into soil watered with acetaminophen (100 mg kg‒1). The measurements of the foliage characteristics, photosynthetic pigments (chlorophylls and carotenoids), the antioxidant capacity, and the amount of the acetaminophen accumulated in the plants were determined in the first and second month of the plant�s exposure to acetaminophen. In the case of P. vulgaris, β-carotene decreased by 57.74% in the first month and continued the same decreasing trend in the second month of exposure by 58.55%. The acetaminophen decreased the chlorophylls content by 7.08% and 13.19% in comparison with the control plants. In case of T. aestivum plants, the photosynthetic parameters decreased up to 44.80% compared to control plants, and the carotenoids decreased up to 53.82%. Acetaminophen was detected in P. vulgaris stems at a concentration of 0.44 �g g‒1 FW (after the first month of exposure) and at 0.15 �g g‒1 FW (after the second month of exposure). The study results indicated a significantly reduction of foliage physiological activity caused by the acetaminophen, suggesting an alteration of the plants antioxidant capacity .


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