scholarly journals Development of the custom polymeric materials specific for aflatoxin B1 and ochratoxin A for application with the ToxiQuant T1 sensor tool

2010 ◽  
Vol 1217 (16) ◽  
pp. 2543-2547 ◽  
Author(s):  
Elena Piletska ◽  
Kal Karim ◽  
Raymond Coker ◽  
Sergey Piletsky
Keyword(s):  
Ochratoxin A ◽  
Aflatoxin B1 ◽  
Polymeric Materials

scholarly journals Occurrence of mycotoxins in selected agricultural and commercial products available in eastern Poland

2021 ◽  
Vol 19 (1) ◽  
pp. 653-664
Author(s):  
Grażyna Kowalska ◽  
Radosław Kowalski
Keyword(s):  
Ochratoxin A ◽  
Aflatoxin B1 ◽  
Extraction Method ◽  
Maximum Allowable Concentration ◽  
Commercial Products ◽  
Ms Analysis ◽  
Rye Flour ◽  
Three Samples ◽  
Aflatoxin B2

Abstract The objective of this study was the estimation of the content of 13 mycotoxins (diacetoxyscirpenol, T-2 toxin, HT-2 toxin, nivalenol, deoxynivalenol, 3-acetyldeoxynivalenol, fusarenone X, aflatoxin B1, aflatoxin B2, aflatoxin G1, aflatoxin G2, ochratoxin A, and zearalenone) in various products from the eastern part of Poland. The content of mycotoxins in the analysed samples was assayed using the extraction method combined with HPLC-MS/MS analysis. We found mycotoxins in 25 of the 92 samples tested (27%). Contamination with mycotoxins was noted most frequently in samples of cereals – 56% – and also in samples of flour and cocoa, in which a content of mycotoxins was noted in 24 and 16% of the samples, respectively. The most frequently identified were the following – deoxynivalenol detected in 18 samples (72%), zearalenone detected in eight samples (32%), toxin HT-2 detected in four samples (16%), ochratoxin A identified in three samples (12%), and toxin T-2 detected in one sample (4%). In one analysed sample of mixed flour and in one analysed sample of wheat and rye flour, the maximum allowable concentration was exceeded in the case of two identified mycotoxins – deoxynivalenol (2,250 μg/kg) and ochratoxin A (15.6 and 17.1 μg/kg).

Impact of maximum levels in European legislation on exposure of mycotoxins in dried products: Case of aflatoxin B1 and ochratoxin A in nuts and dried fruits

2015 ◽  
Vol 75 ◽  
pp. 112-117 ◽  
Author(s):  
Evelien Van de Perre ◽  
Liesbeth Jacxsens ◽  
Carl Lachat ◽  
Fouad El Tahan ◽  
Bruno De Meulenaer
Keyword(s):  
Ochratoxin A ◽  
Aflatoxin B1 ◽  
Dried Fruits ◽  
European Legislation

MYCOTOXINS AND TOXIN-PRODUCING MICROSCOPIC FUNGI IN COTTON-SEED CAKE FROM TAJIKISTAN

2019 ◽  
Vol 1 (1) ◽  
pp. 31-38
Author(s):  
G. P. Kononenko ◽  
◽  
E. A. Piryazeva ◽  
E. V. Zotova ◽  
Sh. I. Razokov ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Aflatoxin B1 ◽  
Cotton Seed ◽  
Practical Significance ◽  
Microscopic Fungi ◽  
Seed Cake ◽  
Complex Study ◽  
The Republic ◽  
Toxin Formation

The article presents the results of complex study of cotton-seed cake from the Republic of Tajikistan, including the determination of content of mycotoxins, the analysis of exposure to microscopic fungi and the assessment of the potential of their toxin formation. The ability to produce mycotoxins was established for 11 species of micromycetes belonging to the genus Aspergillus, Penicillium, Fusarium, Scop-ulariopsis, and Trichothecium. Alternariol, zearalenone, fu-monisins, aflatoxin B1, sterigmatocystin, ochratoxin A, citrinin and emodin were found in the composition of the cotton-seed cake. Prospects for further research and the practical significance of the results are discussed.

scholarly journals Mycotoxins in Tea: Occurrence, Methods of Determination and Risk Evaluation

Toxins ◽  
2018 ◽  
Vol 10 (11) ◽  
pp. 444 ◽  
Author(s):  
Irina Sedova ◽  
Mariya Kiseleva ◽  
Victor Tutelyan
Keyword(s):  
Ochratoxin A ◽  
Aflatoxin B1 ◽  
Health Hazard ◽  
Black Tea ◽  
Contamination Level ◽  
Protection Measures ◽  
Commodity Production ◽  
Exposure Estimate ◽  
Contamination Levels ◽  
Public Health Protection

Tea is one of the most popular beverages all over the world. Being an everyday drink for almost everyone, for centuries tea was considered safe and healthy. However, fungal contamination of tea at any stage of commodity production can pose a serious health hazard due to the accumulation of toxic secondary metabolites of moulds. Contemporary research revealed incidences of highly contaminated samples. Mycotoxin transfer from naturally contaminated raw tea into beverage was well studied for ochratoxin A only, and the possible leak of other mycotoxins is discussed. The results of several surveys were combined to evaluate aflatoxin B1 and ochratoxin A contamination levels in black tea and Pu-erh. Exposure estimate to aflatoxin B1 and ochratoxin A due to tea consumption was carried out based on these data. Average contamination level corresponds to the exposure of 3–40% (aflatoxin B1) and 5–24% (ochratoxin A) of mean overall estimates for different cluster diets. Lack of data does not allow the conclusion for the necessity of public health protection measures. It is necessary to perform representative studies of different kinds of tea for regulated mycotoxins at least. Contemporary techniques for analysis of mycotoxins in tea are summarised in the present review.

Occurrence of aflatoxin B1, citrinin and ochratoxin A in rice in five provinces of the central region of Vietnam

2007 ◽  
Vol 105 (1) ◽  
pp. 42-47 ◽  
Author(s):  
M NGUYEN ◽  
M TOZLOVANU ◽  
T TRAN ◽  
A PFOHLLESZKOWICZ
Keyword(s):  
Ochratoxin A ◽  
Central Region ◽  
Aflatoxin B1

scholarly journals Simultaneous Rapid Detection of Aflatoxin B1 and Ochratoxin A in Spices Using Lateral Flow Immuno-Chromatographic Assay

Foods ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2738
Author(s):  
Xue Zhao ◽  
Xindi Jin ◽  
Zhang Lin ◽  
Qi Guo ◽  
Bin Liu ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Aflatoxin B1 ◽  
High Performance ◽  
Simultaneous Detection ◽  
High Sensitivity ◽  
Strong Stability ◽  
Test Strip ◽  
Chromatography Analysis ◽  
Relative Standard ◽  
Strip Test

Spices are susceptible to contamination by aflatoxin B1 (AFB1) and ochratoxin A (OTA), which are both mycotoxins with high toxicity and carcinogenicity. In this study, we aimed to develop an immuno-chromatographic strip test for the simultaneous quantification of AFB1 and OTA in spices by spraying the coupled antigens AFB1–ovalbumin (AFB1–OVA) and OTA–ovalbumin (OTA–OVA) on a nitrocellulose membrane. The test strip had high sensitivity, good specificity, and strong stability. The detection limits of these two mycotoxins in Chinese prickly ash, pepper, chili, cinnamon, and aniseed were 5 μg/kg. The false positivity rate was 2%, and the false negativity rate was 0%. The maximum coefficient of variation was 4.28% between batches and 5.72% within batches. The average recovery rates of AFB1 and OTA in spices were 81.2–113.7% and 82.2–118.6%, respectively, and the relative standard deviation (RSD) was <10%. The actual sample detection was consistent with high performance liquid chromatography analysis results. Therefore, the immuno-chromatographic test strips developed in this study can be used for the on-site simultaneous detection of AFB1 and OTA in spices. This method would allow the relevant regulatory agencies to strengthen supervision in an effort to reduce the possible human health hazards of such contaminated spices.

scholarly journals Aflatoxin B1, ochratoxin A and zearalenone in sorghum grains marketed in Tunisia

2018 ◽  
Vol 11 (2) ◽  
pp. 103-110 ◽  
Author(s):  
Amani Lahouar ◽  
Ines Jedidi ◽  
Vicente Sanchis ◽  
Salem Saïd
Keyword(s):  
Ochratoxin A ◽  
Aflatoxin B1 ◽  
Sorghum Grains

Immunoassays for Analysis of Mycotoxins

1984 ◽  
Vol 47 (7) ◽  
pp. 562-569 ◽  
Author(s):  
FUN SUN CHU
Keyword(s):  
Ochratoxin A ◽  
Aflatoxin B1 ◽  
Recent Progress ◽  
Biological Fluids ◽  
Aflatoxin M1 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Specific Antibodies ◽  
Protein Conjugates ◽  
Advantages And Disadvantages ◽  
The Past

During the past few years, several laboratories have prepared specific antibodies against aflatoxins B1, M1, B2a and Q1, ochratoxin A, T-2 toxin, and zearalenone. These antibodies were obtained from rabbits after immunizing with various mycotoxin-protein conjugates. With the availability of these antibodies, specific, simple and sensitive radioimmunoassay (RIA) and enzyme-linked immunosorbent assay (ELISA) procedures for monitoring mycotoxins and their metabolites in foods, feeds and body fluids have been developed. In this review, details are presented for the preparation of antibodies and the application of RIA and ELISA to determine aflatoxins B1 and M1, ochratoxin A and T-2 toxin in corn, peanuts, milk and other biological fluids. The sensitivity of ELISA for analysis of these mycotoxins in foods varied from 0.1 μg/L for aflatoxin M1 in milk to 5 μg/kg of aflatoxin B1 in peanuts. The advantages and disadvantages of ELISA for monitoring mycotoxins in foods and feeds are discussed. In addition, a description of recent progress on simplified clean-up procedures which may increase the sensitivity of immunoassays is presented.

Rapid Thin Layer Chromatographic Method for Determining Aflatoxin Bi, Ochratoxin A, and Zearalenone in Corn

1978 ◽  
Vol 61 (3) ◽  
pp. 584-585
Author(s):  
Ivan Balzer ◽  
Čedo Bogdanić ◽  
Stjepan Pepeljnjak
Keyword(s):  
Sodium Bicarbonate ◽  
Thin Layer ◽  
Ochratoxin A ◽  
Ultraviolet Light ◽  
Aflatoxin B1 ◽  
Chromatographic Method ◽  
Limits Of Detection ◽  
Chloroform Extraction ◽  
Tlc Plates

Abstract A multimycotoxin thin layer chromatographic method is described for the analysis of corn. Aflatoxins are extracted from the samples with acetonitrile-water, and sodium bicarbonate is added to separate the acidic ochratoxin from zearalenone and aflatoxin B1. After chloroform extraction, 1/V NaOH is added to separate zearalenone and aflatoxin B1# The separated mycotoxins are spotted on TLC plates, which are then examined under ultraviolet light. The following recoveries ( % ) were obtained for corn samples: aflatoxin B1 71, ochratoxin A 87, and zearalenone 85. The limits of detection for the respective mycotoxins were 2, 40, and 200 ppb.

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