Mg.ATP-decorated ultrafine magnetic nanofibers: A bone scaffold with high osteogenic and antibacterial properties in the presence of an electromagnetic field

Abstract The repair of infective bone defects is a great challenge in clinical work. It is of vital importance to develop a kind of bone scaffold with good osteogenic properties and long-term antibacterial activity for local anti-infection and bone regeneration. A porous mineralized collagen (MC) scaffold containing poly(d,l-lactide-co-glycolic acid) (PLGA) microspheres loaded with two antibacterial synthetic peptides, Pac-525 or KSL-W was developed and characterized via scanning electron microscopy (SEM), porosity measurement, swelling and mechanical tests. The results showed that the MC scaffold embedded with smooth and compact PLGA microspheres had a positive effect on cell growth and also had antibacterial properties. Through toxicity analysis, cell morphology and proliferation analysis and alkaline phosphatase evaluation, the antibacterial scaffolds showed excellent biocompatibility and osteogenic activity. The antibacterial property evaluated with Staphylococcus aureus and Escherichia coli suggested that the sustained release of Pac-525 or KSL-W from the scaffolds could inhibit the bacterial growth aforementioned in the long term. Our results suggest that the antimicrobial peptides-loaded MC bone scaffold has good antibacterial and osteogenic activities, thus providing a great promise for the treatment of infective bone defects.

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GS5-1 EFFECT OF ELECTROMAGNETIC FIELD STIMULATION ON THE MECHANICAL PROPERTIES OF TISSUE-ENGINEERED BONE CONSTRUCTED WITH A CALCINED BOVINE TRABECULAR BONE SCAFFOLD(GS5: Tissue Engineering)

The Proceedings of the Asian Pacific Conference on Biomechanics emerging science and technology in biomechanics ◽

10.1299/jsmeapbio.2015.8.167 ◽

2015 ◽

Vol 2015.8 (0) ◽

pp. 167

Author(s):

Shigeo Tanaka ◽

Yuki Yamashita

Keyword(s):

Mechanical Properties ◽

Tissue Engineering ◽

Electromagnetic Field ◽

Trabecular Bone ◽

Field Stimulation ◽

Bone Scaffold

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Bioactivity and Antibacterial Behaviors of Nanostructured Lithium-Doped Hydroxyapatite for Bone Scaffold Application

International Journal of Molecular Sciences ◽

10.3390/ijms22179214 ◽

2021 ◽

Vol 22 (17) ◽

pp. 9214 ◽

Cited By ~ 2

Author(s):

Pardis Keikhosravani ◽

Hossein Maleki-Ghaleh ◽

Amir Kahaie Khosrowshahi ◽

Mahdi Bodaghi ◽

Ziba Dargahi ◽

...

Keyword(s):

Antibacterial Properties ◽

Xrd Analysis ◽

Bone Scaffold ◽

Cell Analysis ◽

Cellular Behavior ◽

E Coli ◽

Li Doping ◽

Cold Pressed ◽

And Staphylococcus Aureus ◽

Li Content

The material for bone scaffold replacement should be biocompatible and antibacterial to prevent scaffold-associated infection. We biofunctionalized the hydroxyapatite (HA) properties by doping it with lithium (Li). The HA and 4 Li-doped HA (0.5, 1.0, 2.0, 4.0 wt.%) samples were investigated to find the most suitable Li content for both aspects. The synthesized nanoparticles, by the mechanical alloying method, were cold-pressed uniaxially and then sintered for 2 h at 1250 °C. Characterization using field-emission scanning electron microscopy (FE-SEM) revealed particle sizes in the range of 60 to 120 nm. The XRD analysis proved the formation of HA and Li-doped HA nanoparticles with crystal sizes ranging from 59 to 89 nm. The bioactivity of samples was investigated in simulated body fluid (SBF), and the growth of apatite formed on surfaces was evaluated using SEM and EDS. Cellular behavior was estimated by MG63 osteoblast-like cells. The results of apatite growth and cell analysis showed that 1.0 wt.% Li doping was optimal to maximize the bioactivity of HA. Antibacterial characteristics against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) were performed by colony-forming unit (CFU) tests. The results showed that Li in the structure of HA increases its antibacterial properties. HA biofunctionalized by Li doping can be considered a suitable option for the fabrication of bone scaffolds due to its antibacterial and unique bioactivity properties.

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The Effect of Additional Zinc Oxide to Antibacterial Property of Chitosan/Collagen-Based Scaffold

Materials Science Forum ◽

10.4028/www.scientific.net/msf.1000.107 ◽

2020 ◽

Vol 1000 ◽

pp. 107-114

Author(s):

Rowi Alfata ◽

Ghiska Ramahdita ◽

Akhmad Herman Yuwono

Keyword(s):

Zinc Oxide ◽

Functional Group ◽

Porous Scaffold ◽

Antibacterial Properties ◽

Heating Process ◽

Bone Scaffold ◽

Thermally Induced ◽

Sem Image ◽

Lower Porosity ◽

Porosity Characterization

The bone scaffold is susceptible to infection in its application due to the bacteria that often appear on the surface. To prevent this phenomenon, the scaffolds need to be modified in order to provide antibacterial properties. In this study, the bone scaffold was fabricated from chitosan-collagen with the addition of zinc oxide as an antibacterial agent. There were four variables of the amount of zinc oxide added to the scaffold: 0%, 1%, 3%, and 5%. The method used was Thermally Induced Phase Separation (TIPS). From this study, a porous scaffold with a rough surface was obtained. SEM image of the scaffold showed that more zinc oxide caused smaller pore and lower porosity. Characterization with FTIR proved that the scaffold obtained from this process has the same functional group as chitosan and collagen. The DSC-TGA curve confirmed that the heating process performed on dehydrothermal treatment (DHT) did not cause degradation of the scaffold because chitosan and collagen have higher degradation temperatures than DHT temperatures. In addition, this study also proved that the addition of zinc oxide had successfully provided the scaffold with antibacterial properties in which the protection against bacteria was related to the amount of zinc oxide in direct proportion.

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Vancomycin Loaded Alginate/Cockle Shell Powder Nanobiocomposite Bone Scaffold for Antibacterial and Drug Release Evaluation

Sains Malaysiana ◽

10.17576/jsm-2021-5008-14 ◽

2021 ◽

Vol 50 (8) ◽

pp. 2309-2318

Author(s):

Su Wen Yuan ◽

Jacinta Santhanam ◽

Shiow Fern Ng ◽

B. Hemabarathy Bharatham

Keyword(s):

Drug Delivery ◽

Drug Release ◽

Staphylococcus Epidermidis ◽

Biofilm Formation ◽

Antibacterial Properties ◽

Local Drug Delivery ◽

Bone Scaffold ◽

Significant Difference ◽

Shell Powder ◽

Cockle Shell

Bacterial infection and biofilm formation is a major concern in orthopaedic implants and bone reconstructive surgery complications that may be addressed with localized drug delivery system. The potential use of a fabricated nanobiocomposite bone scaffold using alginate and nano cockle shell powder for drug release and antibacterial properties was investigated. Vancomycin loaded bone scaffolds were fabricated with 3 and 5 wt% vancomycin, respectively, while a non-drug loaded scaffold was used as controls. The mineralization of the scaffolds using simulated body fluid (SBF) as well as biofilm formation were evaluated using microscopic observations. Drug release study and antimicrobial activity of the eluent from each sampling period was tested for growth inhibition of Staphylococcus aureus and Staphylococcus epidermidis for a period of 21 days. Significant difference of cumulative amount of vancomycin eluted from scaffolds loaded with 5 wt% vancomycin compared to 3 wt% (p<0.05) were noted. Eluent from both groups showed inhibitory effect against bacterial strain tested for 21 days. The findings are further supported with histological observations of reduced biofilm formation by Staphylococcus epidermidis on surface of 5 wt% vancomycin loaded scaffolds compared to control scaffolds. Basic mineralization studies conducted showed no alteration in drug loaded scaffolds characteristics compared to control scaffolds. Findings from this study indicates antibacterial properties can be conferred to the fabricated bone scaffold with successful incorporation of vancomycin with potentials to be used for local drug delivery application.

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