scholarly journals Fabrication of a nanoparticle-containing 3D porous bone scaffold with proangiogenic and antibacterial properties

2019 ◽  
Vol 86 ◽  
pp. 441-449 ◽  
Author(s):  
Juan L. Paris ◽  
Nuria Lafuente-Gómez ◽  
M. Victoria Cabañas ◽  
Jesús Román ◽  
Juan Peña ◽  
...  
Keyword(s):  
Antibacterial Properties ◽  
Bone Scaffold

scholarly journals Development of an antimicrobial peptide-loaded mineralized collagen bone scaffold for infective bone defect repair

2020 ◽  
Vol 7 (5) ◽  
pp. 515-525
Author(s):  
Yuzhu He ◽  
Yahui Jin ◽  
Xiaoxia Ying ◽  
Qiong Wu ◽  
Shenglian Yao ◽  
...  
Keyword(s):  
Antibacterial Properties ◽  
Clinical Work ◽  
Bone Defects ◽  
Mechanical Tests ◽  
Great Promise ◽  
Plga Microspheres ◽  
Bone Scaffold ◽  
Defect Repair ◽  
Mineralized Collagen

Abstract The repair of infective bone defects is a great challenge in clinical work. It is of vital importance to develop a kind of bone scaffold with good osteogenic properties and long-term antibacterial activity for local anti-infection and bone regeneration. A porous mineralized collagen (MC) scaffold containing poly(d,l-lactide-co-glycolic acid) (PLGA) microspheres loaded with two antibacterial synthetic peptides, Pac-525 or KSL-W was developed and characterized via scanning electron microscopy (SEM), porosity measurement, swelling and mechanical tests. The results showed that the MC scaffold embedded with smooth and compact PLGA microspheres had a positive effect on cell growth and also had antibacterial properties. Through toxicity analysis, cell morphology and proliferation analysis and alkaline phosphatase evaluation, the antibacterial scaffolds showed excellent biocompatibility and osteogenic activity. The antibacterial property evaluated with Staphylococcus aureus and Escherichia coli suggested that the sustained release of Pac-525 or KSL-W from the scaffolds could inhibit the bacterial growth aforementioned in the long term. Our results suggest that the antimicrobial peptides-loaded MC bone scaffold has good antibacterial and osteogenic activities, thus providing a great promise for the treatment of infective bone defects.

scholarly journals Bioactivity and Antibacterial Behaviors of Nanostructured Lithium-Doped Hydroxyapatite for Bone Scaffold Application

2021 ◽  
Vol 22 (17) ◽  
pp. 9214 ◽  
Author(s):  
Pardis Keikhosravani ◽  
Hossein Maleki-Ghaleh ◽  
Amir Kahaie Khosrowshahi ◽  
Mahdi Bodaghi ◽  
Ziba Dargahi ◽  
...  
Keyword(s):  
Antibacterial Properties ◽  
Xrd Analysis ◽  
Bone Scaffold ◽  
Cell Analysis ◽  
Cellular Behavior ◽  
E Coli ◽  
Li Doping ◽  
Cold Pressed ◽  
And Staphylococcus Aureus ◽  
Li Content

The material for bone scaffold replacement should be biocompatible and antibacterial to prevent scaffold-associated infection. We biofunctionalized the hydroxyapatite (HA) properties by doping it with lithium (Li). The HA and 4 Li-doped HA (0.5, 1.0, 2.0, 4.0 wt.%) samples were investigated to find the most suitable Li content for both aspects. The synthesized nanoparticles, by the mechanical alloying method, were cold-pressed uniaxially and then sintered for 2 h at 1250 °C. Characterization using field-emission scanning electron microscopy (FE-SEM) revealed particle sizes in the range of 60 to 120 nm. The XRD analysis proved the formation of HA and Li-doped HA nanoparticles with crystal sizes ranging from 59 to 89 nm. The bioactivity of samples was investigated in simulated body fluid (SBF), and the growth of apatite formed on surfaces was evaluated using SEM and EDS. Cellular behavior was estimated by MG63 osteoblast-like cells. The results of apatite growth and cell analysis showed that 1.0 wt.% Li doping was optimal to maximize the bioactivity of HA. Antibacterial characteristics against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) were performed by colony-forming unit (CFU) tests. The results showed that Li in the structure of HA increases its antibacterial properties. HA biofunctionalized by Li doping can be considered a suitable option for the fabrication of bone scaffolds due to its antibacterial and unique bioactivity properties.

The Effect of Additional Zinc Oxide to Antibacterial Property of Chitosan/Collagen-Based Scaffold

2020 ◽  
Vol 1000 ◽  
pp. 107-114
Author(s):  
Rowi Alfata ◽  
Ghiska Ramahdita ◽  
Akhmad Herman Yuwono
Keyword(s):  
Zinc Oxide ◽  
Functional Group ◽  
Porous Scaffold ◽  
Antibacterial Properties ◽  
Heating Process ◽  
Bone Scaffold ◽  
Thermally Induced ◽  
Sem Image ◽  
Lower Porosity ◽  
Porosity Characterization

The bone scaffold is susceptible to infection in its application due to the bacteria that often appear on the surface. To prevent this phenomenon, the scaffolds need to be modified in order to provide antibacterial properties. In this study, the bone scaffold was fabricated from chitosan-collagen with the addition of zinc oxide as an antibacterial agent. There were four variables of the amount of zinc oxide added to the scaffold: 0%, 1%, 3%, and 5%. The method used was Thermally Induced Phase Separation (TIPS). From this study, a porous scaffold with a rough surface was obtained. SEM image of the scaffold showed that more zinc oxide caused smaller pore and lower porosity. Characterization with FTIR proved that the scaffold obtained from this process has the same functional group as chitosan and collagen. The DSC-TGA curve confirmed that the heating process performed on dehydrothermal treatment (DHT) did not cause degradation of the scaffold because chitosan and collagen have higher degradation temperatures than DHT temperatures. In addition, this study also proved that the addition of zinc oxide had successfully provided the scaffold with antibacterial properties in which the protection against bacteria was related to the amount of zinc oxide in direct proportion.

scholarly journals Vancomycin Loaded Alginate/Cockle Shell Powder Nanobiocomposite Bone Scaffold for Antibacterial and Drug Release Evaluation

2021 ◽  
Vol 50 (8) ◽  
pp. 2309-2318
Author(s):  
Su Wen Yuan ◽  
Jacinta Santhanam ◽  
Shiow Fern Ng ◽  
B. Hemabarathy Bharatham
Keyword(s):  
Drug Delivery ◽  
Drug Release ◽  
Staphylococcus Epidermidis ◽  
Biofilm Formation ◽  
Antibacterial Properties ◽  
Local Drug Delivery ◽  
Bone Scaffold ◽  
Significant Difference ◽  
Shell Powder ◽  
Cockle Shell

Bacterial infection and biofilm formation is a major concern in orthopaedic implants and bone reconstructive surgery complications that may be addressed with localized drug delivery system. The potential use of a fabricated nanobiocomposite bone scaffold using alginate and nano cockle shell powder for drug release and antibacterial properties was investigated. Vancomycin loaded bone scaffolds were fabricated with 3 and 5 wt% vancomycin, respectively, while a non-drug loaded scaffold was used as controls. The mineralization of the scaffolds using simulated body fluid (SBF) as well as biofilm formation were evaluated using microscopic observations. Drug release study and antimicrobial activity of the eluent from each sampling period was tested for growth inhibition of Staphylococcus aureus and Staphylococcus epidermidis for a period of 21 days. Significant difference of cumulative amount of vancomycin eluted from scaffolds loaded with 5 wt% vancomycin compared to 3 wt% (p<0.05) were noted. Eluent from both groups showed inhibitory effect against bacterial strain tested for 21 days. The findings are further supported with histological observations of reduced biofilm formation by Staphylococcus epidermidis on surface of 5 wt% vancomycin loaded scaffolds compared to control scaffolds. Basic mineralization studies conducted showed no alteration in drug loaded scaffolds characteristics compared to control scaffolds. Findings from this study indicates antibacterial properties can be conferred to the fabricated bone scaffold with successful incorporation of vancomycin with potentials to be used for local drug delivery application.

Medicinal Plants: Antibacterial Effects and Chemical Composition of Essential Oil of Foeniculum vulgare

2017 ◽  
Vol 8 (01) ◽  
Author(s):  
Azadeh Foroughi ◽  
Pouya Pournaghi ◽  
Fariba Najafi ◽  
Akram Zangeneh ◽  
Mohammad Mahdi Zangeneh ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Medicinal Plants ◽  
Essential Oil ◽  
Antibacterial Properties ◽  
Screen Test ◽  
Antibacterial Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
Resistant Bacteria ◽  
Antibacterial Effects ◽  
Foeniculum Vulgare

Medicinal plants are considered modern resources for producing agents that could act as alternatives to antibiotics in demeanor of antibiotic-resistant bacteria. The aim of the study was to evaluate the chemical composition and antibacterial activities of essential oil of Foeniculum vulgare (FV) against Pseudomonas aeruginosa and Bacillus subtilis. Gas chromatography mass spectrometry was done to specify chemical composion. As a screen test to detect antibacterial properties of the essential oil, agar disk and agar well diffusion methods were employed. Macrobroth tube test was performed to determinate MIC. The results indicated that the most substance found in FV essential oil was Trans-anethole (47.41 %), also the essential oil of FV with 0.007 g/ml concentration has prevented P. aeruginosa and with 0.002 g/ml concentration has prevented B. subtilis from the growth. Thus, the research represents the antibacterial effects of the medical herb on test P. aeruginosa and B. subtilis. We believe that the article provide support to the antibacterial properties of the essential oil. The results indicate the fact that the essential oil from the plant can be useful as medicinal or preservatives composition.

Garlic Extract (Allium sativum L.) Effectively Inhibits Staphylococcus aureus and Escherichia coli by Invitro Test

2020 ◽  
Vol 2 (2) ◽  
pp. 61-68
Author(s):  
Agnina Listya Anggraini ◽  
Ratih Dewi Dwiyanti ◽  
Anny Thuraidah
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Bacterial Infections ◽  
Allium Sativum ◽  
Antibacterial Properties ◽  
Herbal Medicines ◽  
Garlic Extract ◽  
Dilution Method ◽  
Initial Stage

Infection is a disease caused by the presence of pathogenic microbes, including Staphylococcus aureus and Escherichia coli. Garlic (Allium sativum L.) has chemical contents such as allicin, alkaloids, flavonoids, saponins, tannins, and steroids, which can function as an antibacterial against Staphylococcus aureus and Escherichia coli. This study aims to determine the antibacterial properties of garlic extract powder against Staphylococcus aureus and Escherichia coli. This research is the initial stage of the development of herbal medicines to treat Staphylococcus aureus and Escherichia coli infections. The antibacterial activity test was carried out by the liquid dilution method. The concentrations used were 30 mg/mL, 40 mg/mL, 50 mg/mL, 60 mg/mL and 70 mg/mL. The results showed that the Minimum Inhibitory Concentration (MIC) against Staphylococcus aureus and Escherichia coli was 40 mg/mL and 50 mg / mL. Minimum Bactericidal Concentration (MBC) results for Staphylococcus aureus and Escherichia coli are 50 mg/mL and 70 mg/mL. Based on the Simple Linear Regression test, the R2 value of Staphylococcus aureus and Escherichia coli is 0.545 and 0.785, so it can be concluded that there is an effect of garlic extract powder on the growth of Staphylococcus aureus and Escherichia coli by 54.5% and 78.5%. Garlic (Allium sativum L.) extract powder has potential as herbal medicine against bacterial infections but requires further research to determine its effect in vivo.

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