Identification of microRNAs in response to low potassium stress in the shoots of Tibetan wild barley and cultivated

Nitrogen (N) availability and form have a dramatic effect on N uptake and assimilation in plants, affecting growth and development. In the previous studies, we found great differences in low-N tolerance between Tibetan wild barley accessions and cultivated barley varieties. We hypothesized that there are different responses to N forms between the two kinds of barleys. Accordingly, this study was carried out to determine the response of four barley genotypes (two wild, XZ16 and XZ179; and two cultivated, ZD9 andHua30) under 4Nforms (NO3−, NH4+, urea and glycine). The results showed significant reduction in growth parameters such as root/shoot length and biomass, as well as photosynthesis parameters and total soluble protein content under glycine treatment relative to other N treatments, for both wild and cultivated barley, however, XZ179 was least affected. Similarly, ammonium adversely affected growth parameters in both wild and cultivated barleys, with XZ179 being severely affected. On the other hand, both wild and cultivated genotypes showed higher biomass, net photosynthetic rate, chlorophyll and protein in NO3− treatment relative to other three N treatments. It may be concluded that barley undisputedly grows well under inorganic nitrogen (NO3−), however in response to the organic N wild barley prefer glycine more than cultivated barely.

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Transcriptomic analysis reveals adaptive strategies to chronic low nitrogen in Tibetan wild barley

BMC Plant Biology ◽

10.1186/s12870-019-1668-3 ◽

2019 ◽

Vol 19 (1) ◽

Cited By ~ 3

Author(s):

Xiaoyan Quan ◽

Jianbin Zeng ◽

Guang Chen ◽

Guoping Zhang

Keyword(s):

Wild Barley ◽

Adaptive Strategies ◽

Transcriptomic Analysis ◽

Tibetan Wild Barley ◽

Low Nitrogen

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Ionomic, metabolomic and proteomic analyses reveal molecular mechanisms of root adaption to salt stress in Tibetan wild barley

Plant Physiology and Biochemistry ◽

10.1016/j.plaphy.2017.12.032 ◽

2018 ◽

Vol 123 ◽

pp. 319-330 ◽

Cited By ~ 13

Author(s):

Qiufang Shen ◽

Jiahua Yu ◽

Liangbo Fu ◽

Liyuan Wu ◽

Fei Dai ◽

...

Keyword(s):

Salt Stress ◽

Molecular Mechanisms ◽

Wild Barley ◽

Tibetan Wild Barley ◽

Proteomic Analyses

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Characteristics of Photosynthetic Performance, Antioxidant Capacity and Nutrient Concentration of Tibetan Wild Barley in Response to Aluminium Stress

Asian Journal of Chemistry ◽

10.14233/ajchem.2013.14582 ◽

2013 ◽

Vol 25 (14) ◽

pp. 7727-7731 ◽

Cited By ~ 1

Author(s):

Huaxin Dai ◽

Wasim Ibrahim ◽

Wei-Te Zheng ◽

Muhammad Dawood ◽

Xiaoyan He ◽

...

Keyword(s):

Antioxidant Capacity ◽

Nutrient Concentration ◽

Wild Barley ◽

Photosynthetic Performance ◽

Tibetan Wild Barley

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Physiological and antioxidant responses of cultivated and wild barley under salt stress

Plant Soil and Environment ◽

10.17221/169/2020-pse ◽

2020 ◽

Vol 66 (No. 7) ◽

pp. 334-344

Author(s):

Zahra Jabeen ◽

Nazim Hussain ◽

Faiza Irshad ◽

Jianbin Zeng ◽

Ayesha Tahir ◽

...

Keyword(s):

Dna Damage ◽

Salt Stress ◽

Wild Barley ◽

Antioxidant Defense System ◽

Oxidative Capacity ◽

Plant Tissues ◽

Salt Tolerant ◽

Antioxidant Responses ◽

Tibetan Wild Barley ◽

High Degree

Saline soil is a critical environmental problem affecting crop yield worldwide. Tibetan wild barley is distinguished for its vast genetic diversity and high degree of tolerance to abiotic stress, including salinity. The present study compared the response of antioxidant defense system in the XZ16 wild and CM72 cultivated barleys to salt stress. Wild barley was relatively more tolerant than cultivated CM72, salt-tolerant cultivar, with less Na+ uptake and more K+, Ca2+, and Mg2+ retention in plant tissues. The results of diaminobenzidine (DAB) and nitroblue tetrazolium (NBT) staining showed that XZ16 had significantly lower H2O2 and O2− concentrations than a salt-sensitive cultivar Gairdner, suggesting that the salt-tolerant genotype suffer from less oxidative damage. Moreover, XZ16 and Gairdner had the highest and lowest anti-oxidative enzyme activities and proline content in plant tissues. In addition, the microscopic examination revealed that DNA damage in cv. Gairdner was closely correlated to oxidative stress, representing that more reactive oxygen species accumulation in plants tissues leads to subsequent DNA damage. The present results show that higher salt tolerance of wild barley XZ16 is attributed to less Na+ accumulation and stronger anti-oxidative capacity.

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The Barley S-Adenosylmethionine Synthetase 3 Gene HvSAMS3 Positively Regulates the Tolerance to Combined Drought and Salinity Stress in Tibetan Wild Barley

Cells ◽

10.3390/cells9061530 ◽

2020 ◽

Vol 9 (6) ◽

pp. 1530

Author(s):

Imrul Mosaddek Ahmed ◽

Umme Aktari Nadira ◽

Cheng-Wei Qiu ◽

Fangbin Cao ◽

Zhong-Hua Chen ◽

...

Keyword(s):

Salinity Stress ◽

Wild Barley ◽

Leaf Tissue ◽

Secretory Protein ◽

Antioxidant Enzyme Activities ◽

Virus Induced Gene Silencing ◽

Salt Tolerant ◽

Tibetan Wild Barley ◽

Drought Tolerant ◽

Adenosylmethionine Synthetase

Drought and salinity are two of the most frequently co-occurring abiotic stresses. Despite recent advances in the elucidation of the effects of these stresses individually during the vegetative stage of plants, significant gaps exist in our understanding of the combined effects of these two frequently co-occurring stresses. Here, Tibetan wild barley XZ5 (drought tolerant), XZ16 (salt tolerant), and cultivated barley cv. CM72 (salt tolerant) were subjected to drought (D), salinity (S), or a combination of both treatments (D+S). Protein synthesis is one of the primary activities of the green part of the plant. Therefore, leaf tissue is an important parameter to evaluate drought and salinity stress conditions. Sixty differentially expressed proteins were identified by mass spectrometry (MALDI-TOF/TOF) and classified into 9 biological processes based on Gene Ontology annotation. Among them, 21 proteins were found to be expressed under drought or salinity alone; however, under D+S, 7 proteins, including S-adenosylmethionine synthetase 3 (SAMS3), were exclusively upregulated in drought-tolerant XZ5 but not in CM72. HvSAMS3 carries both N-terminal and central domains compared with Arabidopsis and activates the expression of several ethylene (ET)-responsive transcription factors. HvSAMS3 is mainly expressed in the roots and stems, and HvSAMS3 is a secretory protein located in the cell membrane and cytoplasm. Barley stripe mosaic virus-based virus-induced gene silencing (BSMV-VIGS) of HvSAMS3 in XZ5 severely compromised its tolerance to D+S and significantly reduced plant growth and K+ uptake. The reduced tolerance to the combined stress was associated with the inhibition of polyamines such as spermidine and spermine, polyamine oxidase, ethylene, biotin, and antioxidant enzyme activities. Furthermore, the exogenous application of ethylene and biotin improved the tolerance to D+S in BSMV-VIGS:HvSAMS3-inoculated plants. Our findings highlight the significance of HvSAMS3 in the tolerance to D+S in XZ5.

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