scholarly journals The T Box Transcription Factor No Tail in Ciliated Cells Controls Zebrafish Left-Right Asymmetry

2004 ◽  
Vol 14 (8) ◽  
pp. 685-690 ◽  
Author(s):  
Jeffrey D Amack ◽  
H.Joseph Yost
Keyword(s):  
Transcription Factor ◽  
Ciliated Cells ◽  
T Box ◽  
No Tail ◽  
Left Right Asymmetry

Molecular identification of spadetail: regulation of zebrafish trunk and tail mesoderm formation by T-box genes

Development ◽  
1998 ◽  
Vol 125 (17) ◽  
pp. 3379-3388 ◽  
Author(s):  
K.J. Griffin ◽  
S.L. Amacher ◽  
C.B. Kimmel ◽  
D. Kimelman
Keyword(s):  
Transcription Factor ◽  
Genetic Model ◽  
Zebrafish Embryos ◽  
Fibroblast Growth ◽  
Fgf Signaling ◽  
T Box ◽  
Tail Development ◽  
No Tail ◽  
Mesoderm Formation ◽  
Complementary Actions

Inhibition of fibroblast growth factor (FGF) signaling prevents trunk and tail formation in Xenopus and zebrafish embryos. While the T-box transcription factor Brachyury (called No Tail in zebrafish) is a key mediator of FGF signaling in the notochord and tail, the pathways activated by FGF in non-notochordal trunk mesoderm have been uncertain. Previous studies have shown that the spadetail gene is required for non-notochordal trunk mesoderm formation; spadetail mutant embryos have major trunk mesoderm deficiencies, but relatively normal tail and notochord development. We demonstrate here that spadetail encodes a T-box transcription factor with homologues in Xenopus and chick. Spadetail is likely to be a key mediator of FGF signaling in trunk non-notochordal mesoderm, since spadetail expression is regulated by FGF signaling. Trunk and tail development are therefore dependent upon the complementary actions of two T-box genes, spadetail and no tail. We show that the regulatory hierarchy among spadetail, no tail and a third T-box gene, tbx6, are substantially different during trunk and tail mesoderm formation, and propose a genetic model that accounts for the regional phenotypes of spadetail and no tail mutants.

scholarly journals The Transcription Factor RFX3 Directs Nodal Cilium Development and Left-Right Asymmetry Specification

2004 ◽  
Vol 24 (10) ◽  
pp. 4417-4427 ◽  
Author(s):  
E. Bonnafe ◽  
M. Touka ◽  
A. AitLounis ◽  
D. Baas ◽  
E. Barras ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Immune System ◽  
Intraflagellar Transport ◽  
Body Axis ◽  
High Rate ◽  
Ciliated Cells ◽  
Wild Type ◽  
Left Right Asymmetry ◽  
First Time ◽  
Deficient Mice

ABSTRACT There are five members of the RFX family of transcription factors in mammals. While RFX5 plays a well-defined role in the immune system, the functions of RFX1 to RFX4 remain largely unknown. We have generated mice with a deletion of the Rfx3 gene. RFX3-deficient mice exhibit frequent left-right (LR) asymmetry defects leading to a high rate of embryonic lethality and situs inversus in surviving adults. In vertebrates, specification of the LR body axis is controlled by monocilia in the embryonic node, and defects in nodal cilia consequently result in abnormal LR patterning. Consistent with this, Rfx3 is expressed in ciliated cells of the node and RFX3-deficient mice exhibit a pronounced defect in nodal cilia. In contrast to the case for wild-type embryos, for which we document for the first time a twofold increase in the length of nodal cilia during development, the cilia are present but remain markedly stunted in mutant embryos. Finally, we show that RFX3 regulates the expression of D2lic, the mouse orthologue of a Caenorhabditis elegans gene that is implicated in intraflagellar transport, a process required for the assembly and maintenance of cilia. In conclusion, RFX3 is essential for the differentiation of nodal monocilia and hence for LR body axis determination.

scholarly journals Assessing the role of the T-box transcription factor Eomes in B cell differentiation during either Th1 or Th2 cell-biased responses

PLoS ONE ◽  
2018 ◽  
Vol 13 (12) ◽  
pp. e0208343 ◽  
Author(s):  
Lucy Cooper ◽  
Lauren Hailes ◽  
Amania Sheikh ◽  
Colby Zaph ◽  
Gabrielle T. Belz ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Cell Differentiation ◽  
B Cell ◽  
B Cell Differentiation ◽  
T Box ◽  
Th2 Cell

scholarly journals The T Box Transcription Factor TBX2 Promotes Epithelial-Mesenchymal Transition and Invasion of Normal and Malignant Breast Epithelial Cells

PLoS ONE ◽  
2012 ◽  
Vol 7 (7) ◽  
pp. e41355 ◽  
Author(s):  
Bin Wang ◽  
Linsey E. Lindley ◽  
Virneliz Fernandez-Vega ◽  
Megan E. Rieger ◽  
Andrew H. Sims ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Epithelial Cells ◽  
Epithelial Mesenchymal Transition ◽  
Breast Epithelial Cells ◽  
Mesenchymal Transition ◽  
T Box ◽  
Malignant Breast ◽  
Breast Epithelial

scholarly journals High expression level of T-box transcription factor 5 predicts unfavorable survival in stage I and II gastric adenocarcinoma

2015 ◽  
Vol 10 (4) ◽  
pp. 2021-2026 ◽  
Author(s):  
YAN ZHENG ◽  
YUAN-FANG LI ◽  
WEI WANG ◽  
YONG-MING CHEN ◽  
DAN-DAN WANG ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Gastric Adenocarcinoma ◽  
Stage I ◽  
High Expression ◽  
Expression Level ◽  
T Box ◽  
High Expression Level

scholarly journals Multiple malignant tumors in a patient with familial chordoma, a case report

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Nuttavut Sumransub ◽  
Paari Murugan ◽  
Shelly Marette ◽  
Denis R. Clohisy ◽  
Keith M. Skubitz
Keyword(s):  
Transcription Factor ◽  
Cell Carcinoma ◽  
Germ Line ◽  
Malignant Tumors ◽  
Treatment Resistance ◽  
Pulmonary Complications ◽  
Her Family ◽  
T Box ◽  
Epithelial Cancers ◽  
Pleomorphic Sarcoma

Abstract Background Chordoma is a rare bone tumor that is typically resistant to chemotherapy and is associated with genetic abnormalities of the T-box transcription factor T (TBXT) gene, which encodes the transcription factor brachyury. Brachyury is felt to be a major contributor to the development of chordomas. Case presentation We describe a 67-year-old woman who developed an undifferentiated pleomorphic sarcoma in her thigh. Despite treatment with standard chemotherapy regimens, she had a rapidly progressive course of disease with pulmonary metastases and passed away 8 months from diagnosis with pulmonary complications. Her medical history was remarkable in that she had a spheno-occipital chordoma at age 39 and later developed multiple other tumors throughout her life including Hodgkin lymphoma and squamous cell carcinoma and basal cell carcinoma of the skin. She had a family history of chordoma and her family underwent extensive genetic study in the past and were found to have a duplication of the TBXT gene. Conclusions Brachyury has been found to associate with tumor progression, treatment resistance, and metastasis in various epithelial cancers, and it might play roles in tumorigenesis and aggressiveness in this patient with multiple rare tumors and germ line duplication of the TBXT gene. Targeting this molecule may be useful for some malignancies.

The zebrafish T-box genesno tailandspadetailare required for development of trunk and tail mesoderm and medial floor plate

Development ◽  
2002 ◽  
Vol 129 (14) ◽  
pp. 3311-3323 ◽  
Author(s):  
Sharon L. Amacher ◽  
Bruce W. Draper ◽  
Brian R. Summers ◽  
Charles B. Kimmel
Keyword(s):  
Embryonic Development ◽  
Neural Tube ◽  
Transcriptional Regulators ◽  
Floor Plate ◽  
Wild Type ◽  
T Box ◽  
No Tail ◽  
Ventral Neural Tube ◽  
Plate Formation ◽  
In Cells

T-box genes encode transcriptional regulators that control many aspects of embryonic development. Here, we demonstrate that the mesodermally expressed zebrafish spadetail (spt)/VegT and no tail (ntl)/Brachyury T-box genes are semi-redundantly and cell-autonomously required for formation of all trunk and tail mesoderm. Despite the lack of posterior mesoderm in spt–;ntl– embryos, dorsal-ventral neural tube patterning is relatively normal, with the notable exception that posterior medial floor plate is completely absent. This contrasts sharply with observations in single mutants, as mutations singly in ntl or spt enhance posterior medial floor plate development. We find that ntl function is required to repress medial floor plate and promote notochord fate in cells of the wild-type notochord domain and that spt and ntl together are required non cell-autonomously for medial floor plate formation, suggesting that an inducing signal present in wild-type mesoderm is lacking in spt–;ntl– embryos.

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