Methacrylate-functionalized proanthocyanidins as novel polymerizable collagen cross-linkers – Part 1: Efficacy in dentin collagen bio-stabilization and cross-linking

2021 ◽  
Author(s):  
Viviane Hass ◽  
Yong Li ◽  
Rong Wang ◽  
Dung Nguyen ◽  
Zhonghua Peng ◽  
...  
Keyword(s):  
Cross Linking ◽  
Dentin Collagen

scholarly journals Characterization of Genipin-Modified Dentin Collagen

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Hiroko Nagaoka ◽  
Hideaki Nagaoka ◽  
Ricardo Walter ◽  
Lee W. Boushell ◽  
Patricia A. Miguez ◽  
...  
Keyword(s):  
Amino Acid ◽  
Lysyl Oxidase ◽  
Biomechanical Properties ◽  
Biochemical Properties ◽  
Cross Linking ◽  
Dependent Manner ◽  
Cross Link ◽  
Concentration Dependent Manner ◽  
Cross Links ◽  
Dentin Collagen

Application of biomodification techniques to dentin can improve its biochemical and biomechanical properties. Several collagen cross-linking agents have been reported to strengthen the mechanical properties of dentin. However, the characteristics of collagen that has undergone agent-induced biomodification are not well understood. The objective of this study was to analyze the effects of a natural cross-linking agent, genipin (GE), on dentin discoloration, collagen stability, and changes in amino acid composition and lysyl oxidase mediated natural collagen cross-links. Dentin collagen obtained from extracted bovine teeth was treated with three different concentrations of GE (0.01%, 0.1%, and 0.5%) for several treatment times (0–24 h). Changes in biochemical properties of NaB3H4-reduced collagen were characterized by amino acid and cross-link analyses. The treatment of dentin collagen with GE resulted in a concentration- and time-dependent pigmentation and stability against bacterial collagenase. The lysyl oxidase-mediated trivalent mature cross-link, pyridinoline, showed no difference among all groups while the major divalent immature cross-link, dehydro-dihydroxylysinonorleucine/its ketoamine in collagen treated with 0.5% GE for 24 h, significantly decreased compared to control (P< 0.05). The newly formed GE-induced cross-links most likely involve lysine and hydroxylysine residues of collagen in a concentration-dependent manner. Some of these cross-links appear to be reducible and stabilized with NaB3H4.

scholarly journals Efficacy of new natural biomodification agents from Anacardiaceae extracts on dentin collagen cross-linking

2017 ◽  
Vol 33 (10) ◽  
pp. 1103-1109 ◽  
Author(s):  
M.A. Moreira ◽  
N.O. Souza ◽  
R.S. Sousa ◽  
D.Q. Freitas ◽  
M.V. Lemos ◽  
...  
Keyword(s):  
Cross Linking ◽  
Dentin Collagen ◽  
Collagen Cross Linking

scholarly journals Theaflavins as a novel cross-linker quickly stabilize demineralized dentin collagen against degradation

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Hang Liu ◽  
Jing Guo ◽  
Rong Wang ◽  
Yong Wang
Keyword(s):  
Enzymatic Degradation ◽  
Black Tea ◽  
Grape Seed ◽  
Transmission Electron Microscopy Data ◽  
Cross Linking ◽  
Grape Seed Proanthocyanidins ◽  
Scanning Transmission ◽  
Dentin Collagen ◽  
Cross Linker ◽  
Collagen Cross Linking

AbstractTo investigate the ability of theaflavins (TF) from black tea to protect dentin collagen against enzymatic degradation via cross-linking effect under clinically relevant conditions. 10-µm-thick dentin films were microtomed from dentin slabs of human molars. Following demineralization, films or slabs were treated with TF at two concentrations (0.4% and 2%) for 30 s. A well-known collagen cross-linker grape seed proanthocyanidins (PA) was used as control. Collagen cross-linking interactions and stabilization against enzymatic degradation were investigated by Fourier transform infrared spectroscopy, weight loss, hydroxyproline release, and scanning/transmission electron microscopy. Data were analyzed by ANOVA, Tukey’s and Student’s T test (α = 0.05%). The results showed collagen cross-linking and stabilization efficacy was dependent on TF/PA concentrations. At 2.0%, TF and PA offered nearly full protection to collagen; at 0.4%, TF exhibited a significantly better collagen stabilization effect than PA (P < 0.05), while untreated collagen was completely digested. It’s concluded that TF cross-links dentin collagen within a clinically relevant time (30 s) and offers excellent collagen protection against enzymatic degradation, with efficacy comparable to or better than PA. The study supports the potential use of TF as a novel, promising collagen cross-linker for degradation resistant, long-lasting dentin bonding in composite restorations.

scholarly journals Characterization of a Conjugate between Rose Bengal and Chitosan for Targeted Antibiofilm and Tissue Stabilization Effects as a Potential Treatment of Infected Dentin

2012 ◽  
Vol 56 (9) ◽  
pp. 4876-4884 ◽  
Author(s):  
Annie Shrestha ◽  
Michael R. Hamblin ◽  
Anil Kishen
Keyword(s):  
Mechanical Properties ◽  
Rose Bengal ◽  
Structural Changes ◽  
Structural Integrity ◽  
Green Light ◽  
Cross Linking ◽  
Antibiofilm Activity ◽  
Content Type ◽  
Dentin Collagen

ABSTRACTBacterial biofilms and dentin structural changes are some of the major challenges in the management of infected dentin tissue. This study characterized a photosensitizer-conjugated chitosan with enhanced photodynamic efficacy against dental biofilms, as well as the ability to reinforce the postinfected dentin matrix in order to improve its mechanical and chemical stability. Rose Bengal-conjugated chitosan (CSRB) was synthesized using a chemical cross-linking method and characterized for photophysical, photobiological, and cytotoxicity properties. Its potential as an antibacterial and matrix-reinforcing agent on dentin collagen was also evaluated.Enterococcus faecalisas planktonic andin vitrobiofilms was treated with CSRB and photodynamically activated with 5 to 60 J/cm2green light. Dentin collagen was used for the CSRB cross-linking experiments and evaluated for chemical changes, resistance to enzymatic degradation, and mechanical properties. CSRB was a photosensitizer with efficient singlet oxygen yield.In vitrophotoactivation gave higher fibroblast cell survival than did RB alone. CSRB showed significant antibiofilm photoinactivation (P< 0.01). The CSRB-cross-linked dentin collagen showed higher resistance to collagenase degradation and superior mechanical properties (P< 0.05). In summary, the photoactivated CSRB particles synthesized in this study may be a synergistic multifunctional treatment approach with lower cytotoxicity and effective antibiofilm activity as well as the ability to reinforce the dentin collagen to enhance resistance to degradation and improve mechanical properties. This may be a targeted treatment strategy to deal with infected dentin hard tissues in a clinical scenario, where both disinfection and structural integrity need to be addressed concomitantly.

scholarly journals Cranberry Juice Extract Rapidly Protects Demineralized Dentin against Digestion and Inhibits Its Gelatinolytic Activity

Materials ◽  
2021 ◽  
Vol 14 (13) ◽  
pp. 3637
Author(s):  
Yong Wang ◽  
Austin Green ◽  
Xiaomei Yao ◽  
Hang Liu ◽  
Saleha Nisar ◽  
...  
Keyword(s):  
Grape Seed ◽  
Dental Composite ◽  
Cross Linking ◽  
Cranberry Juice ◽  
Composite Restorations ◽  
Dentin Collagen ◽  
Cross Linker ◽  
The Cross ◽  
Demineralized Dentin

Improving the longevity of composite restorations has proven to be difficult when they are bonded to dentin. Dentin demineralization leaves collagen fibrils susceptible to enzymatic digestion, which causes breakdown of the resin–dentin interface. Therefore, measures for counteracting the enzymatic environment by enhancing dentin collagen’s resistance to degradation have the potential to improve the durability of dental composite restorations. This study aimed to evaluate the effects of polyphenol-rich extracts and a chemical cross-linker on the cross-linking interaction, resistance to digestion, and endogenous matrix metalloproteinase (MMP) activities of dentin collagen under clinically relevant conditions. Ten-µm-thick films were cut from dentin slabs of non-carious extracted human third molars. Following demineralization, polyphenol-rich extracts—including grape seed (GSE), green tea (GTE), and cranberry juice (CJE)—or chemical cross-linker carbodiimide with n-hydroxysuccinimide (EDC/NHS) were applied to the demineralized dentin surfaces for 30 s. The collagen cross-linking, bio-stabilization, and gelatinolytic activities of MMPs 2 and 9 were studied by using Fourier-transform infrared spectroscopy, weight loss, hydroxyproline release, scanning/transmission electron microscopy, and in situ zymography. All treatments significantly increased resistance to collagenase degradation and reduced the gelatinolytic MMP activity of dentin collagen compared to the untreated control. The CJE- and GSE-treated groups were more resistant to digestion than the GTE- or EDC/NHS-treated ones (p < 0.05), which was consistent with the cross-linking interaction found with FTIR and the in situ performance on the acid-etched dentin surface found with SEM/TEM. The collagen films treated with CJE showed the lowest MMP activity, followed by GSE, GTE, and, finally, EDC/NHS. The CJE-treated dentin collagen rapidly increased its resistance to digestion and MMP inhibition. An application of CJE as short as 30 s may be a clinically feasible approach to improving the longevity of dentin bonding in composite restorations.

Occlusal loading and cross-linking effects on dentin collagen degradation in physiological conditions

2016 ◽  
Vol 32 (2) ◽  
pp. 192-199 ◽  
Author(s):  
Gianluca Turco ◽  
Andrea Frassetto ◽  
Luca Fontanive ◽  
Annalisa Mazzoni ◽  
Milena Cadenaro ◽  
...  
Keyword(s):  
Collagen Degradation ◽  
Cross Linking ◽  
Physiological Conditions ◽  
Dentin Collagen

scholarly journals Effect of tea root-derived proanthocyanidin fractions on protection of dentin collagen

2019 ◽  
Vol 48 (5) ◽  
pp. 030006051989130
Author(s):  
Honglin Yang ◽  
Bingqing Xie ◽  
Yue Wang ◽  
Yayun Cui ◽  
Hui Yang ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Butyl Alcohol ◽  
Degree Of Polymerization ◽  
Cross Linking ◽  
Protective Mechanisms ◽  
Dentin Collagen ◽  
And Function ◽  
High Degree ◽  
Collagen Cross Linking

Objectives Proanthocyanidins (PAs) have been widely used as effective agents for dentin collagen cross-linking to enhance the biomechanics and biostability of dentin in vitro. However, the effects and protective mechanisms of various tea root-derived PA components on dentin remain undefined. This study evaluated the effects of these tea root-derived PA components on dentin biomechanics and biostability. Methods In this study, ethyl acetate and n-butyl alcohol were used to extract PAs with different degrees of polymerization from tea roots; the effects of these PA extracts on dentin were evaluated. Results Dentin was treated with glutaraldehyde, ethyl acetate, n-butyl alcohol, or water. PAs with a high degree of polymerization, extracted using n-butyl alcohol, were able to more effectively improve dentin collagen cross-linking, increase resistance to bacterial collagenase digestion, and enhance dentin elasticity, relative to treatment with glutaraldehyde or PAs with a low degree of polymerization (extracted using ethyl acetate). Additionally, treatment with aqueous extract of tea roots was detrimental to dentin stability and function. Conclusions PAs with a high degree of polymerization were effective for dentin protection and restoration in vitro, suggesting clinical treatment potential for tea root-derived PAs.

scholarly journals Effects of Chemical Cross-linkers on Caries-affected Dentin Bonding

2009 ◽  
Vol 88 (12) ◽  
pp. 1096-1100 ◽  
Author(s):  
G.V. Macedo ◽  
M. Yamauchi ◽  
A.K. Bedran-Russo
Keyword(s):  
Bond Strength ◽  
Dental Materials ◽  
Grape Seed Extract ◽  
Grape Seed ◽  
Seed Extract ◽  
Structure Stability ◽  
Cross Linking ◽  
Dentin Collagen ◽  
Bond Strengths ◽  
Collagen Stability

The achievement of a strong and stable bond between composite resin and dentin remains a challenge in restorative dentistry. Over the past two decades, dental materials have been substantially improved, with better handling and bonding characteristics. However, little attention has been paid to the contribution of collagen structure/stability to bond strength. We hypothesized that the induction of cross-linking in dentin collagen improves dentin collagen stability and bond strength. This study investigated the effects of glutaraldehyde-and grape seed extract-induced cross-linking on the dentin bond strengths of sound and caries-affected dentin, and on the stability of dentin collagen. Our results demonstrated that the application of chemical cross-linking agents to etched dentin prior to bonding procedures significantly enhanced the dentin bond strengths of caries-affected and sound dentin. Glutaraldehyde and grape seed extract significantly increased dentin collagen stability in sound and caries-affected dentin, likely via distinct mechanisms.

Influence of Calcium Ions on the Plant Cell Surface: Membrane Fusions and Conformational Changes

1974 ◽  
Vol 32 ◽  
pp. 154-155
Author(s):  
D. James Morré ◽  
Charles E. Bracker ◽  
William J. VanDerWoude
Keyword(s):  
Cell Wall ◽  
Cell Surface ◽  
Conformational Changes ◽  
Calcium Ions ◽  
Surface Membrane ◽  
Carboxyl Groups ◽  
Cross Linking ◽  
Ultrastructural Evidence ◽  
Glycine Max L ◽  
Wall Extensibility

Calcium ions in the concentration range 5-100 mM inhibit auxin-induced cell elongation and wall extensibility of plant stems. Inhibition of wall extensibility requires that the tissue be living; growth inhibition cannot be explained on the basis of cross-linking of carboxyl groups of cell wall uronides by calcium ions. In this study, ultrastructural evidence was sought for an interaction of calcium ions with some component other than the wall at the cell surface of soybean (Glycine max (L.) Merr.) hypocotyls.

Embedding Procedure for Water Swollen Samples

1970 ◽  
Vol 28 ◽  
pp. 504-505
Author(s):  
Ann M. Thomas ◽  
Virginia Shemeley
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Ion Exchange ◽  
Structural Changes ◽  
Cross Linking ◽  
Ion Exchange Resins ◽  
Transmission Electron ◽  
First Pass ◽  
Exchange Resins

Those samples which swell rapidly when exposed to water are, at best, difficult to section for transmission electron microscopy. Some materials literally burst out of the embedding block with the first pass by the knife, and even the most rapid cutting cycle produces sections of limited value. Many ion exchange resins swell in water; some undergo irreversible structural changes when dried. We developed our embedding procedure to handle this type of sample, but it should be applicable to many materials that present similar sectioning difficulties.The purpose of our embedding procedure is to build up a cross-linking network throughout the sample, while it is in a water swollen state. Our procedure was suggested to us by the work of Rosenberg, where he mentioned the formation of a tridimensional structure by the polymerization of the GMA biproduct, triglycol dimethacrylate.

Sign in / Sign up

Export Citation Format

Share Document