CHM-1 induces apoptosis via p38-mediated upregulation of DR5 expression in human ovarian cancer SKOV3 cells

ObjectiveAplasia Ras homolog member I (ARHI) is associated with human ovarian cancer (HOC) growth and proliferation; however, the mechanisms are unclear. The purpose of this study was to investigateARHIeffects in HOC SKOV3 cells.MethodsWe transfected SKOV3 cells with PIRES2-EGFP-ARHI and measured growth inhibition rates, cell cycle distribution, apoptosis rates, and expression of P-STAT3 (phosphorylated signal transduction and activators of transcription 3) and P-ERK (phosphorylated extracellular signal regulated protein kinase).ResultsOur data showed significant inhibition of growth, significantly increased S-phase arrest and apoptosis rates, and reduction of P-STAT3 and P-ERK1/2 expression levels.ConclusionsWe propose the mechanism may involveARHI-induced phosphorylation of ERK1/2 and STAT3 protein kinases, thereby blocking proliferation signaling pathways, to induce HOC SKOV3 apoptosis.

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GRP137 promotes cell proliferation and metastasis through regulation of the PI3K/AKT pathway in human ovarian cancer

Tumori Journal ◽

10.5301/tj.5000703 ◽

2018 ◽

Vol 104 (5) ◽

pp. 330-337 ◽

Cited By ~ 4

Author(s):

Li-qian Zhang ◽

Su-qing Yang ◽

Xiang-dong Qu ◽

Xian-jun Chen ◽

Hong-sheng Lu ◽

...

Keyword(s):

Ovarian Cancer ◽

G Protein ◽

Biological Activities ◽

Xenograft Model ◽

Akt Pathway ◽

Ovarian Cancer Cells ◽

Human Ovarian Cancer ◽

G Protein Coupled Receptor ◽

Skov3 Cells ◽

G Protein Coupled

Purpose: Ovarian cancer is one of the leading causes of death for women worldwide. The present study aims to investigate the role of G protein-coupled receptor 137 (GPR137) in the biological activities of ovarian cancer cells. Methods: (QUERY: Please supply Methods for Abstract) Results: G protein-coupled receptor 137 was highly expressed in clinical ovarian cancer tissues and exhibited the highest protein levels in SKOV3 cells and OVCAR3 cells. Knockdown of GPR137 caused significant decreases in cell proliferative rates and colony formation abilities in SKOV3 cells and OVCAR3 cells and also inhibited the in vivo tumorigenesis in a xenograft model. It was observed that knockdown of GPR137 inhibited cell motility by up to 40% in SKOV3 cells and approximately 65% in OVCAR3 cells in wound-healing assay. Cell migration abilities were consistently inhibited by 68.2% in SKOV3 cells and 59.3% in OVCAR3 cells, whereas cell invasion abilities were inhibited by 64.0% and 74.2% in SKOV3 and OVCAR3 cells, respectively, after knockdown of GPR137. When GPR137 was depleted, epithelial markers were increased, while mesenchymal markers decreased. Conclusions: Our data suggest that GPR137 plays pro-oncogenic roles in ovarian cancer via regulation of the PI3K/AKT pathway. These observations might pave new insights into therapeutic strategies against human ovarian cancer.

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Effects of arsenic trioxide on migration and invasion of human ovarian cancer SKOV3 cells and study on related molecular mechanism

Pharmaceutical Care and Research ◽

10.5428/pcar20190204 ◽

2019 ◽

Vol 19 (2) ◽

pp. 93-97

Author(s):

Peng ZHOU ◽

◽

Yan WANG ◽

Gaoxiang HUANG ◽

Yidong LI

Keyword(s):

Ovarian Cancer ◽

Arsenic Trioxide ◽

Molecular Mechanism ◽

Migration And Invasion ◽

Human Ovarian Cancer ◽

Skov3 Cells

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METCAM/MUC18 is a novel tumor and metastasis suppressor for the human ovarian cancer SKOV3 cells

BMC Cancer ◽

10.1186/s12885-016-2181-9 ◽

2016 ◽

Vol 16 (1) ◽

Cited By ~ 10

Author(s):

Guang-Jer Wu ◽

Guo-fang Zeng

Keyword(s):

Ovarian Cancer ◽

Metastasis Suppressor ◽

Human Ovarian Cancer ◽

Skov3 Cells

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Calycosin induces apoptosis in human ovarian cancer SKOV3 cells by activating caspases and Bcl-2 family proteins

Tumor Biology ◽

10.1007/s13277-015-3194-8 ◽

2015 ◽

Vol 36 (7) ◽

pp. 5333-5339 ◽

Cited By ~ 15

Author(s):

Yan Zhou ◽

Qing-Hua Liu ◽

Chun-Lei Liu ◽

Li Lin

Keyword(s):

Ovarian Cancer ◽

Human Ovarian Cancer ◽

Skov3 Cells

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Effect of Juglone on Migration of Human Ovarian Cancer SKOV3 Cells

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.912-914.1911 ◽

2014 ◽

Vol 912-914 ◽

pp. 1911-1914

Author(s):

Liang Zhong Zhao ◽

Shuang Chen ◽

Qing Fang ◽

Duo Zhang ◽

You Peng Zhu ◽

...

Keyword(s):

Ovarian Cancer ◽

Wound Healing ◽

Plant Species ◽

Human Ovarian Cancer ◽

Skov3 Cell ◽

Potential Candidate ◽

Wound Healing Assay ◽

Skov3 Cells ◽

Anti Cancer ◽

Cancer Activity

Juglone is isolated from many plant species belonging to Juglandaceae family. Recent studies have shown that Juglone exhibits various bioactivities including anti-tumor functions. However, its anti-cancer activity on human ovarian cancer SKOV3 cell has not been examined. Thus, the current study was designed to elucidate the effect of Juglone on migration of human ovarian cancer SKOV3 cells. In the present study, SKOV3 cells were incubated with Juglone at various concentrations. Wound healing assay and Transwell chambers were used to detect migration of SKOV3 treated with Juglone for 24h. The result showed that Juglone inhibited the migration of SKOV3 cells with concentration of Juglone at 25, 50 or 100μM compared with control cells. Therefore, our results indicated that Juglone may be a potential candidate of drug for ovarian cancer.

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Induction of mitochondria mediated apoptosis in human ovarian cancer cells by folic acid coated tin oxide nanoparticles

PLoS ONE ◽

10.1371/journal.pone.0258115 ◽

2021 ◽

Vol 16 (10) ◽

pp. e0258115

Author(s):

Demiana H. Hanna ◽

Gamal R. Saad

Keyword(s):

Ovarian Cancer ◽

Cancer Cells ◽

Tin Oxide ◽

Reactive Oxygen Species Generation ◽

Ovarian Cancer Cells ◽

Oxide Nanoparticles ◽

Human Ovarian Cancer ◽

Oxygen Species ◽

Skov3 Cells ◽

Tin Oxide Nanoparticles

Purpose This study aims to prepare folic acid coated tin oxide nanoparticles (FA-SnO2 NPs) for specifically targeting human ovarian cancer cells with minimum side effects against normal cells. Methods The prepared FA-SnO2 NPs were characterized by FT-IR, UV-vis spectroscopy, XRD, SEM and TEM. The inhibition effects of FA-SnO2 NPs against SKOV3 cancer cell were tested by MTT and LDH assay. Apoptosis induction in FA-SnO2 NPs treated SKOV3 cells were investigated using Annexin V/PI, AO/EB and Comet assays and the possible mechanisms of the cytotoxic action were studied by Flow cytometry, qRT-PCR, Immunohistochemistry, and Western blotting analyses. The effects of FA-SnO2 NPs on reactive oxygen species generation in SKOV3 cells were also examined. Additionally, the safety of utilization FA-SnO2 NPs were studied in vivo using Wister rats. Results The obtained FA-SnO2 NPs displayed amorphous spherical morphology with an average diameter of 157 nm and a zeta potential value of -24 mV. Comparing to uncoated SnO2 NPs, FA-SnO2 NPs had a superior inhibition effect towards SKOV3 cell growth that was suggested to be mediated through higher reactive oxygen species generation. It was showed that FA-SnO2 NPs increased significantly the % of apoptotic cells in the sub- G1 and G2/M phases with a higher intensity comet nucleus in SKOV3 treated cells. Furthermore, FA-SnO2 NPs was significantly increased the expression levels of P53, Bax, and cleaved Caspase-3 and accompanied with a significant decrease of Bcl-2 in the treated SKOV3 cells. Conclusion Overall, the results suggested that an increase in cellular FA-SnO2 NPs internalization resulted in a significant induced cytotoxicity in SKOV3 cancer cells in dose-dependent mode through ROS-mediated cell apoptosis that may have occurred through mitochondrial pathway. Additionally, the results confirmed the safety of utilization FA-SnO2 NPs against living systems. So, FA-SnO2 NPs with a specific targeting moiety may be a promising therapeutic candidate for human ovarian cancer.

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Effects of nano-taxol/curcumin on ovarian cancer cells

Materials Express ◽

10.1166/mex.2020.1818 ◽

2020 ◽

Vol 10 (10) ◽

pp. 1615-1619

Author(s):

Shuai Zhang ◽

Junhui Liang ◽

Changzhong Li ◽

Fei Wang

Keyword(s):

Ovarian Cancer ◽

Cell Proliferation ◽

Cancer Cells ◽

Culture Medium ◽

Morphological Characteristics ◽

Ovarian Cancer Cells ◽

Human Ovarian Cancer ◽

Pharmacodynamic Effect ◽

Skov3 Cells ◽

Inhibit Cell Proliferation

To investigate the pharmacodynamic effect of urushin nanoparticles upon the proliferation inhibition in human ovarian cancer SKOV3 cells, and in order to explore their biomechanism, the cell cycle and the percentage of apoptotic cells in human ovarian cancer SKOV3 cells were analyzed utilizing flow cytometry. The concentration of astragalin nanoparticles in SKOV3 cells was identified utilizing HPIC. Consequently, the morphological characteristics of SKOV3 cells in a culture medium of 5 mg/L were investigated and measured. In our findings, the 50 mg cancer cells containing 50 mg IC did not display this noted effect. The results exhibit the discovery that urushin nanoparticles inhibit cell proliferation, which is related to the inhibition of DNA replication and the regulation of the cell proliferation cycle. HPLC results demonstrated that the pharmacological effect of urushin nanoparticles was directly related to the drug concentration present within the studied cells. Hence, urushin nanoparticles can effectively enter cells and then effectively inhibit cell proliferation.

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