n-Butylparaben exposure through gestation and lactation impairs spermatogenesis and steroidogenesis causing reduced fertility in the F1 generation male rats

2020 ◽  
Vol 256 ◽  
pp. 112957 ◽  
Author(s):  
Priyanka Maske ◽  
Vikas Dighe ◽  
Chandrashekhar Mote ◽  
Geeta Vanage
Keyword(s):  
Male Rats ◽  
Generation Male ◽  
F1 Generation

scholarly journals Melatonin Ameliorates Developmental Landmarks Induced by Gestational and Lactational Coexposure to Chlorpyriphos and Cypermethrin in F1 Male Rats

2021 ◽  
Author(s):  
Muftau Shittu ◽  
Suleiman Ambali ◽  
Joseph Ayo ◽  
Umaru Kawu ◽  
Motunrayo Akande
Keyword(s):  
Antioxidant Property ◽  
Male Rats ◽  
Testicular Descent ◽  
Anogenital Distance ◽  
Crown Rump Length ◽  
Lactational Exposure ◽  
Male Wistar Rats ◽  
Soya Oil ◽  
Generation Male ◽  
F1 Generation

Abstract The ameliorative potentials of melatonin (ML) on developmental changes evoked by gestational and lactational co-exposure to chlorpyriphos (CP) and cypermethrin (CY) was investigated in male Wistar rats. Pregnant dams were divided at random into 6 groups of 10 animals each and treated orally by gavage from gestation day 1 to postnatal day 21 with the following regimens: The DW, SO and ML groups were administered distilled water (2 ml/kg), soya oil (2 ml/kg) and melatonin (0.5 mg/kg), respectively; CC group was co-administered CP (1.9 mg/kg) and CY (7.5 mg/kg); MC group was pretreated with ML (0.5 mg/kg) and followed by co-administration of CP and CY while the CM group was administered CP and CY and then treated with ML. We evaluated the developmental parameters on the F1 generation male rats at different postnatal intervals following parturition. Alterations in litter size and weight, number of live/dead pups, anogenital distance, crown-rump length, time of eye and ear openings, and testicular descent induced by gestational and lactational exposure to CP and CY in F1 male rats were mitigated by pre- and post-administration of ML. These curative and prophylactic potentials of ML may be partly attributed to its widely known antioxidant property.

Oral (Drinking Water) Two-Generation Reproductive Toxicity Study of Dibromoacetic Acid (DBA) in Rats

2002 ◽  
Vol 21 (4) ◽  
pp. 237-276 ◽  
Author(s):  
M. S. Christian ◽  
R. G. York ◽  
A. M. Hoberman ◽  
J. Frazee ◽  
L. C. Fisher ◽  
...  
Keyword(s):  
Drinking Water ◽  
Body Weight ◽  
Female Rats ◽  
Male Rats ◽  
Feed Consumption ◽  
Organ Weights ◽  
Body Weights ◽  
Group Generation ◽  
Generation Male ◽  
F1 Generation

In a two-generation study of dibromoacetic acid (DBA), Crl SD rats (30 rats/sex/group/generation) were provided DBA in drinking water at 0 (reverse osmosis-deionized water), 50,250, and 650 ppm (0,4.4 to 11.6,22.4 to 55.6, and 52.4 to 132.0 mg/kg/day, respectively; human intake approximates 0.1 μg/kg/day [0.0001 mg/kg/day]). Observations included viability, clinical signs, water and feed consumption, body and organ weights, histopathology, and reproductive parameters (mating, fertility, abortions, premature deliveries, durations of gestation, litter sizes, sex ratios and viabilities, maternal behaviors, reproductive organ weights, sperm parameters and implantation sites, sexual maturation). Histopathological evaluations were performed on at least 10 P and F1 rats/sex at 0 and 650 ppm (gross lesions, testes, intact epididymis; 10 F1 dams at 0, 250, and 650 ppm for primordial follicles). Developmental observations included implantations, pup numbers, sexes, viabilities, body weights, morphology, and reproductive performance. At 50 ppm and higher, both sexes and generations had increased absolute and relative liver and kidneys weights, and female rats in both generations had reduced absolute and relative adrenal weights; adrenal changes were probably associated with physiological changes in water balance. The livers and kidneys (10/sex/group/generation) had no histopathological changes. Other minimal effects at 50 ppm were reduced water consumption and a transient reduction in body weight. At 250 and 650 ppm, DBA reduced parental water consumption, body weight gains, body weights, feed consumption, and pup body weights. P and F1 generation male rats at 250 and 650 ppm had altered sperm production (retained step 19 spermatids in stages IX and X tubules sometimes associated with residual bodies) and some epididymal tubule changes (increased amounts of exfoliated spermatogenic cells/residual bodies in epididymal tubules, atrophy, and hypospermia), although inconsistently and at much lower incidences. Unilateral abnormalities of the epididymis (small or absent epididymis) at 650 ppm in four F1 generation male rats were considered reproductive tract malformations. The no-observable-adverse-effect level (NOAEL) and reproductive and developmental NOAELs for DBA were at least 50 ppm (4.5 to 11.6 mg/kg/day), 45,000 to 116,000 times the human adult exposure level. Reproductive and developmental effects did not occur in female rats exposed to DBA concentrations as high as 650 ppm. Based on the high multiples of human exposure required to produce effects in male rats, DBA should not be identified as a human reproductive or developmental risk.

Effects of endocrine disruptor furan on reproductive physiology of Sprague Dawley rats: An F1 Extended One-Generation Reproductive Toxicity Study (EOGRTS)

2020 ◽  
Vol 39 (8) ◽  
pp. 1079-1094 ◽  
Author(s):  
H Rehman ◽  
S Jahan ◽  
I Ullah ◽  
P-O Thörnqvist ◽  
M Jabbar ◽  
...  
Keyword(s):  
Reproductive Toxicity ◽  
Head Tilt ◽  
Treated Group ◽  
Toxicity Study ◽  
Female Rats ◽  
Male Rats ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Estrous Cyclicity ◽  
F1 Generation

The present study investigated the reproductive toxicity of furan in an Extended One-Generation Reproductive Toxicity Study in rats. Sprague Dawley F0 weaning rats (30 per sex per group) were exposed to furan orally at 0, 1, 2.5, 5, and 10 mg kg−1 for 10 weeks (males) and 2 weeks (females) and then mated. Results of F0 indicated that in the furan-treated groups (5 mg kg−1 and 10 mg kg−1), body weight (bw) gain decreased during prebreed and gestational period while increased during lactation periods. F0 animals prebreeding exposure resulted in head tilt and foot splay at 10 mg kg−1. Number of live pups at birth were decreased ( p < 0.001) at 10 mg kg−1. At postnatal day (PND) 70, a significant ( p = 0.03) decrease in testosterone levels of male rats and estrogen levels of female rats ( p = 0.05) was observed in 10 mg kg−1 furan-treated group in F1 generation. Luteinizing hormone, follicle-stimulating hormone, and progesterone levels were also reduced, but their reduction was not statistically significant in all groups. In higher dose furan group (10 mg kg−1), testicular and ovarian weights were reduced in F1 generation at PND 70, with decreased daily sperm production ( p = 0.01) and disturbed estrous cyclicity ( p < 0.01). Some histopathological changes were also observed in testis and ovaries in groups whose parents were previously exposed to 10 mg kg−1 bw of furan group. Based on the above results, it is suggested that exposure to food-based contaminant furan induced remarkable changes in the F0 (parental stage) and F1 (offspring, pubertal, and adult stage) generations of Sprague Dawley rats.

Transgenerational effects of a hypercaloric diet

2017 ◽  
Vol 29 (2) ◽  
pp. 325 ◽  
Author(s):  
A. O. Joaquim ◽  
C. P. Coelho ◽  
P. Dias Motta ◽  
E. F. Bondan ◽  
E. Teodorov ◽  
...  
Keyword(s):  
Early Adulthood ◽  
Inflammatory Cells ◽  
Female Rats ◽  
Male Rats ◽  
Transgenerational Effect ◽  
Gfap Immunoreactivity ◽  
Factor Α ◽  
Hypercaloric Diet ◽  
Necrosis Factor ◽  
F1 Generation

The effects of a maternal hypercaloric diet (HD) during puberty and early adulthood on neuroimmune aspects in offspring were investigated. In female rats of the F0 generation and male rats of the F1 generation, bodyweight (BW) gain, retroperitoneal fat (RPF) weight, the number of hypodermic adipocytes (HAs) and expression of glial fibrillary acidic protein (GFAP) were measured in hypothalamic astrocytes. On Postnatal Day 50, the F1 pups were challenged with lipopolysaccharide (LPS, 100 µg kg–1, s.c.) or an equal volume of saline (S), and behaviour in the open field test was evaluated, as were plasma neuropeptide and cytokine concentrations. The maternal HD caused the female F0 rats to become overweight. The F1 offspring of dams fed the HD and challenged with saline (HDS group) exhibited increases in BW gain, RPF weight and in the number of large HAs and a decrease in GFAP immunoreactivity. F1 offspring of dams fed the HD and challenged with LPS (HDLPS group) exhibited decreases in BW gain, RPF weight and GFAP immunoreactivity, but no differences were observed in the number of larger and small HAs. Plasma tumour necrosis factor-α concentrations were high in the HDS and HDLPS groups. Thus, the maternal HD during puberty and early adulthood caused the F1 generation to become overweight despite the fact that they received a normocaloric diet. These results indicate a transgenerational effect of the HD that may occur, in part, through permanent changes in immune system programming. The attenuation of neuroinflammation biomarkers after LPS administration may have resulted in a decrease in the number of adipocytes, which, in turn, reduced cytokine, adipokine and chemokine levels, which are able to recruit inflammatory cells in adipose tissue.

scholarly journals Chromosomal Microarray Analysis on Intergenerational Effects of Oligomeric Proanthocyanidins Against Bisphenol-A Induced Brain Deformities

2021 ◽  
pp. 930-939
Author(s):  
Fatin Naziha Osman ◽  
Fatin Nadzirah Zakaria ◽  
Siti Aishah Zulazlan ◽  
Nooraain Hashim ◽  
Razif Dasiman ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Microarray Analysis ◽  
Female Rats ◽  
Male Rats ◽  
Chromosome Microarray Analysis ◽  
Oligomeric Proanthocyanidins ◽  
Intergenerational Effect ◽  
Chromosome Microarray ◽  
Microarray Cgh ◽  
F1 Generation

Oligomeric Proanthocyanidins (OPC) is a type of polyphenolic compound which have been demonstrated to have anti-cancer, anti-oxidant, anti-inflammatory and anti-mutagenic properties that may have the potential to reduce intergenerational effect of BPA towards DNA. This study was conducted to determine the effect of OPC on the DNA damage of BPA-induced rats using microarray CGH Chromosome Karyotyping. Adult Male Sprague Dawley rats were divided into six groups which are Normal, BPA, OPC10, OPC20, BPA+OPC10 and BPA+OPC20. The administration of BPA and/or OPC were conducted for 21 days using oral gavage before being mated with female rats of the same age at 1:1 mating ratio. Once the female rats were confirmed pregnant, the male rats were decapitated and their blood were collected for chromosome microarray analysis. The male offspring (F1 generation) were allowed to grow until 10 weeks old and their blood were also collected for chromosome microarray analysis. BPA group had a deletion of Vomeronasal receptor genes in which the deletion magnitude increased from P to F1 generation may affect the ability of the rats to detect chemosensory cues during neurobehavioral test. The amplification of Major Histocompatibility complex (MHC) class I gene in BPA+OPC20 group may aid in a better performance during hippocampal-dependent memory tests. These results suggested that OPC could be a potential agent in reducing the intergenerational effect of BPA. Current finding may enrich our understanding particularly in the possible mechanism of OPC on BPA-induced memory impairment   Keywords: Bisphenol-A, Brain, microarray CGH Chromosome Karyotyping, Oligomeric Proanthocyanidins, Intergenerational

scholarly journals Sterilisation of painted apple moth Teia anortoides (Lepidoptera Lymantriidae) by irradiation

2002 ◽  
Vol 55 ◽  
pp. 7-11 ◽  
Author(s):  
D.M. Suckling ◽  
J.K. Hackett ◽  
A.M. Barrington ◽  
J.M. Daly
Keyword(s):  
Wind Tunnel ◽  
Gamma Rays ◽  
Late Stage ◽  
Dispersal Distance ◽  
Sterile Male ◽  
Eradication Programme ◽  
F2 Generation ◽  
Generation Male ◽  
Mating Performance ◽  
F1 Generation

The Australian painted apple moth (Teia anartoides) has been the target of an eradication programme in Auckland This has included an extensive trapping programme underpinned by moth dispersal studies Sterilisation of males was considered essential before release to avoid exacerbating the eradication problem Late stage male pupae were irradiated using 125 MeV gamma rays from a Cobalt60 source at six doses (60 80 100 120 140 and 160 Gy) No effects were measurable on male emergence or mating performance in the treated compared to control insects Significant effects were observed in the F1 generation with increasing doses producing increased mortality At the highest doses 100 sterility was achieved in the F2 generation Male flight in a wind tunnel was not significantly affected by irradiation at 160 Gy Markrelease recapture experiments were successfully conducted with the maximum recorded dispersal distance of several kilometers by irradiated sterile male moths

scholarly journals High-fat diet accelerate hepatic fatty acids synthesis in offspring male rats induced by perinatal exposure to nonylphenol

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Hongyu Zhang ◽  
Chengguang Song ◽  
Rong Yan ◽  
Hongbo Cai ◽  
Yi Zhou ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
High Fat Diet ◽  
First Generation ◽  
Second Generation ◽  
Normal Diet ◽  
Male Rats ◽  
Perinatal Exposure ◽  
High Fat ◽  
Abnormal Lipid Metabolism ◽  
Generation Male

Abstract Background Low dose of NP exposure can alter adipose tissue formation, and the intake of high-fat diet (HFD) can also lead to the fatty liver disease. We investigated the combined effect of NP and HFD on the first offspring of rats, and whether this effect can be passed to the next generation and the possible mechanisms involved. Methods Pregnant rats had access to be treated with 5 μg/kg/day NP and normal diet. The first generation rats were given normal diet and HFD on postnatal day 21, respectively. Then the second generation rats started to only receive normal diet without NP or HFD. Body weight, organ coefficient of liver tissues, lipid profile, biochemical indexes and the expression of genes involved in lipid metabolism, as well as liver histopathology were investigated in male offspring of rats. Results NP and HFD interaction had significant effect on the birth weight, body weight and liver tissue organ coefficient of first generation male rats. And HFD aggravated abnormal lipid metabolism, even abnormal liver function and liver histopathological damage of first generation male rats produced by the NP. And this effect can be passed on to the second generation rats. HFD also accelerated the mRNA level of fatty acid synthesis genes such as Lpl, Fas, Srebp-1 and Ppar-γ of first generation rats induced by perinatal exposure to NP, even passed on to the second generation of male rats. NP and HFD resulted in synergistical decrease of the protein expression level of ERα in liver tissue in F2 male rats. Conclusion HFD and NP synergistically accelerated synthesis of fatty acids in liver of male offspring rats through reducing the expression of ERα, which induced abnormal lipid metabolism, abnormal liver function and hepatic steatosis. Moreover, all of these damage passed on to the next generation rats.

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