scholarly journals High-fat diet accelerate hepatic fatty acids synthesis in offspring male rats induced by perinatal exposure to nonylphenol

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Hongyu Zhang ◽  
Chengguang Song ◽  
Rong Yan ◽  
Hongbo Cai ◽  
Yi Zhou ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
High Fat Diet ◽  
First Generation ◽  
Second Generation ◽  
Normal Diet ◽  
Male Rats ◽  
Perinatal Exposure ◽  
High Fat ◽  
Abnormal Lipid Metabolism ◽  
Generation Male

Abstract Background Low dose of NP exposure can alter adipose tissue formation, and the intake of high-fat diet (HFD) can also lead to the fatty liver disease. We investigated the combined effect of NP and HFD on the first offspring of rats, and whether this effect can be passed to the next generation and the possible mechanisms involved. Methods Pregnant rats had access to be treated with 5 μg/kg/day NP and normal diet. The first generation rats were given normal diet and HFD on postnatal day 21, respectively. Then the second generation rats started to only receive normal diet without NP or HFD. Body weight, organ coefficient of liver tissues, lipid profile, biochemical indexes and the expression of genes involved in lipid metabolism, as well as liver histopathology were investigated in male offspring of rats. Results NP and HFD interaction had significant effect on the birth weight, body weight and liver tissue organ coefficient of first generation male rats. And HFD aggravated abnormal lipid metabolism, even abnormal liver function and liver histopathological damage of first generation male rats produced by the NP. And this effect can be passed on to the second generation rats. HFD also accelerated the mRNA level of fatty acid synthesis genes such as Lpl, Fas, Srebp-1 and Ppar-γ of first generation rats induced by perinatal exposure to NP, even passed on to the second generation of male rats. NP and HFD resulted in synergistical decrease of the protein expression level of ERα in liver tissue in F2 male rats. Conclusion HFD and NP synergistically accelerated synthesis of fatty acids in liver of male offspring rats through reducing the expression of ERα, which induced abnormal lipid metabolism, abnormal liver function and hepatic steatosis. Moreover, all of these damage passed on to the next generation rats.

Effects of Zinc Alpha2 Glycoprotein on Lipid Metabolism of Liver in High-Fat Diet-Induced Obese Mice

2017 ◽  
Vol 49 (10) ◽  
pp. 793-800 ◽  
Author(s):  
Guoqiang Fan ◽  
Yu Qiao ◽  
Shixing Gao ◽  
Jun Guo ◽  
Ruqian Zhao ◽  
...  
Keyword(s):  
Body Weight ◽  
Lipid Metabolism ◽  
High Fat Diet ◽  
Recombinant Plasmid ◽  
Diet Group ◽  
Normal Diet ◽  
Hormone Sensitive Lipase ◽  
High Fat ◽  
Hepatic Lipid ◽  
Protein Levels

AbstractZinc alpha2 glycoprotein (ZAG) is a new type of adipokine involved in adipose tissue mobilization, however, little is known about its lipid metabolism effect in liver. Therefore, we investigated the effects of ZAG in the regulation of hepatic lipid accumulation. Mice were randomly divided into two groups; one was fed a normal diet and another was fed a high-fat diet for eight weeks to establish obesity model. After that, the normal diet group was divided into ND (injection of pcDNA3.1) and NDZ (injection of ZAG recombinant plasmid) and the high-fat diet group was divided into HF (injection of pcDNA3.1) and HFZ (injection of ZAG recombinant plasmid). The mice were weighed once per week and injected with plasmid once every three days for eight times. The results showed that body weight and hepatic TG content were decreased dramatically in HFZ group compared with HF group. The stearoyl-CoAdesaturase1 (SCD1) and Acyl-CoA Synthetase-1 (ACSS1) protein levels in HFZ group were significantly decreased. Furthermore, phosphorylated hormone sensitive lipase (P-HSL) was significantly higher in HFZ group. In HFZ group, hepatic fatty acid translocase (CD36) and fatty acids binding protein-1 (FABP1) protein levels were reduced. In addition, the expression of phosphorylated protein kinase A (PPKA) in HFZ group was higher than the HF group. Meanwhile, NDZ group showed significantly decreased body weight and increased P-HSL level though the hepatic TG content showed no significantly changes compared with the ND group. Therefore, we conclude that ZAG may be beneficial for preventing high-fat-diet-induced hepatic lipid metabolic disorders.

scholarly journals Antiobesity Effect ofCodonopsis lanceolatain High-Calorie/High-Fat-Diet-Induced Obese Rats

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Hye-Kyung Choi ◽  
Eun-Kyung Won ◽  
Young Pyo Jang ◽  
Se-Young Choung
Keyword(s):  
Lipid Accumulation ◽  
High Fat Diet ◽  
Density Lipoprotein ◽  
Normal Diet ◽  
The Body ◽  
Male Rats ◽  
Sprague Dawley ◽  
High Fat ◽  
Codonopsis Lanceolata ◽  
High Calorie

The antiobesity effects ofCodonopsis lanceolata(CL) were evaluated in a high-calorie/high-fat-diet (HFD-) induced obesity rat model and 3T3-L1 cells. The Sprague-Dawley male rats were fed a normal diet (ND) or a HFD for a period of 12 weeks. The rats were subdivided into groups: ND, ND + wildCodonopsis lanceolata(wCL) (900 mg/kg/day, p.o.), ND + cultivatedCodonopsis lanceolata(cCL) (900 mg/kg/day, p.o.), HFD, HFD + wCL (100, 300, or 900 mg/kg/day, p.o.), HFD + cCL (100, 300, or 900 mg/kg/day, p.o.), and HFD + sibutramine. The body weight gains of the administered HFD + CL (wCL or CCL) were lower than those of the rats fed with only the HFD group. Moreover, the weight of adipose pads and the serum levels of triglycerides, total cholesterol, and low density lipoprotein cholesterol in the group administered HDL + CL were significantly lower than in the HFD group. The inhibitory effect of lipid accumulation in 3T3-L1 cells was measured by Oil Red O staining and reverse transcription-polymerase chain reaction (RT-PCR). Treatment of 3T3-L1 cells with wCL inhibited lipid accumulation and expression of C/EBPαand PPARγ. These results suggest that CL has a great potential as a functional food with anti-obesity effects and as a therapeutic alternative in the treatment of obesity.

scholarly journals Sex differences in gut microbiome in high-fat-diet fed rats.

2020 ◽  
Author(s):  
Ying Shi ◽  
Fangzhi Yue ◽  
Lin Xing ◽  
Shanyu Wu ◽  
Lin Wei ◽  
...  
Keyword(s):  
Sex Differences ◽  
Gut Microbiota ◽  
Gut Microbiome ◽  
High Fat Diet ◽  
Metabolic Diseases ◽  
Normal Diet ◽  
Female Rats ◽  
Male Rats ◽  
High Fat ◽  
Alcoholic Fatty Liver

Abstract Background Sex differences in obesity and related metabolic diseases are well recognized, however, the mechanism has not been elucidated. Gut microbiota and its metabolites may play a vital role in the development of obesity and metabolic diseases. The aim of the present study was to investigate sex differences in gut microbiota and its metabolites in a high-fat-diet (HFD) obesity rats and identify microbiota genera potentially contributing to such differences in obesity and non-alcoholic fatty liver disease (NAFLD) susceptibility. Results Sprague–Dawley rats were divided into the following groups (seven animals per group): (1) male rats on a normal diet (MND), (2) male rats on HFD (MHFD), (3) female rats on a normal diet (FND), and (4) female rats on HFD (FHFD). HFD induced more body weight gain and fat storage in female rats, however, lower hepatic steatosis in FHFD than in MHFD rats was observed. When considering gut microbiota composition, FHFD rats had lower microbiome diversity than MHFD. A significant increase of Firmicutes phylum and Bilophila genus was detected in MHFD rats, as compared with FHFD, which showed increased relative abundance of Murimonas and Roseburia . Moreover, propionic and lauric acid levels were higher in FHFD than those in MHFD rats. Conclusion HFD induced sex-related alterations in gut microbiome and fatty acids. Furthermore, the genus Bilophila and Roseburia might contribute to sex differences observed in obesity and NAFLD susceptibility.

scholarly journals The PCSK9 Involves in the Dyslipidemia of Ovariectomized Mice and Its Mechanism

2021 ◽  
Author(s):  
Dan Zhao ◽  
Xue-qin Zhang ◽  
Wen-jing Guo ◽  
Zhi-hui Cui ◽  
Yi-cheng Wang ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
High Fat Diet ◽  
Cholesterol Metabolism ◽  
Critical Role ◽  
Normal Diet ◽  
High Fat ◽  
Lipid Metabolism Disorder ◽  
Pcsk9 Inhibitor ◽  
Metabolism Disorder ◽  
Ovariectomized Mice

Abstract Ovarian failure in postmenopausal female leads E2 to dramatic decrease which is an important reason of menopausal dyslipidemia. PCSK9 as a secretory lipid metabolic regulator plays a critical role in the cholesterol metabolism by negatively regulating LDLR in hepatocytes. Clinical data showed PCSK9 was elevated and positively correlated with LDL-C in the blood of postmenopausal women. However, the relationship between E2 and PCSK9 and the role of PCSK9 in postmenopausal dyslipidemia are still unclear. In this research, 10-week-old ovariectomized mice were fed for 4 weeks with normal diet or high-fat diet, then tested the lipid metabolism profiles and PCSK9 in the blood and the expression of LDLR and PCSK9 in the liver. On this basis, PCSK9-/- ovariectomized mice were used to further verify the effect of PCSK9 in dyslipidemia of ovariectomized mice. Finally, the ovariectomized mice with high-fat diet were subcutaneous injected respectively with E2 or PCSK9 inhibitor alone or both together for 2 weeks and were tested as previous experiment. The results showed PCSK9, TC and LDL-c all increased in the blood of in WT ovariectomized mice and their PCSK9 is positively correlated with LDL-c, while there were on obvious lipid metabolism disorder in the PCSK9−/− ovariectomized mice. PCSK9 inhibitor increased the LDLR on the liver and ameliorated the dyslipidemia in WT ovariectomized mice. It suggests that PCSK9 plays an important role in the dyslipidemia of ovariectomized mice, which provides a new strategy for clinical diagnosis and treatment of the dyslipidemia in post-menopause.

scholarly journals OR-006 Effect of Moderate-intensity Exercise on the Expression of Hypothalamic KiSS-1 and GPR54 mRNA in Diet Induced Obesity Rats

2018 ◽  
Vol 1 (2) ◽  
Author(s):  
Yi Yan ◽  
Chunyu Liang ◽  
Rui Xu
Keyword(s):  
High Fat Diet ◽  
Normal Diet ◽  
Treadmill Training ◽  
Male Rats ◽  
Moderate Intensity ◽  
High Fat ◽  
Moderate Intensity Exercise ◽  
Diet Induced Obesity ◽  
Sd Rats ◽  
Fat Feeding

Objective To observe the effect of high fat diet on the hypothalamic expression of KiSS-1and the G-protein coupled receptor (GPR) 54 mRNA and explore the modulatory role of moderate-intensity exercise in the diet induced obesity male rats. Methods After 8 weeks high fat feeding, 20 obesity 11-weeks SD rats were randomly assigned to high-fat diet sedentary (FS, n=8) and high-fat diet exercise (FE, n=8) groups, 20 normal diet 11-weeks SD rats also were randomly assigned to sedentary (SS, n=8) and exercise (SE, n=8) groups. During the following 8 weeks, obesity rats were continued expose to high-fat-diet. SE and FE groups did the 60%-70%V(•)O2max treadmill training (5 days/week, 1 hour/day). The V(•)O2 max of exercise groups were remeasured every two weeks. The hypothalamic expression of KiSS-1 and GPR54 mRNA were tested in each group. Results After the first 8-weeks high fat feeding, the obesity rats were heavier than normal diet group (491.74±26.19g vs. 410.05±45.77g, p<0.01). After 8-weeks training, the FE group was lighter than FS group (590.23±35.74g vs. 681±52.56, p<0.01). The FS group had higher hypothalamic expression of KiSS-1 mRNA (1.51±0.66 vs 0.75±0.27, p<0.05) and GPR54 mRNA (2.45±0.38 vs 0.61±0.15, p<0.01) than SS group. The FE group had lower hypothalamic expression of KiSS-1 mRNA (0.69±0.13, p>0.05) and GPR54 mRNA (0.58±0.10, p<0.01) than FS group. Conclusions There is stimulating effect of high-fat diet induced obesity on hypothalamic expression of KiSS-1and GPR54 mRNA. 8-weeks 60%-70%V (•) O2max treadmill training could cure this effect.  

scholarly journals Comparative Analysis of Metabolite Profiling of Momordica charantia Leaf and the Anti-Obesity Effect through Regulating Lipid Metabolism

2021 ◽  
Vol 18 (11) ◽  
pp. 5584
Author(s):  
Meiqi Fan ◽  
Jae-In Lee ◽  
Young-Bae Ryu ◽  
Young-Jin Choi ◽  
Yujiao Tang ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Lipid Metabolism ◽  
High Fat Diet ◽  
Density Lipoprotein ◽  
Momordica Charantia ◽  
Lipoprotein Cholesterol ◽  
Distilled Water ◽  
High Fat ◽  
Leaf Extracts ◽  
Abnormal Lipid Metabolism

This study investigated the effects of Momordica charantia (M. charantia) extract in obesity and abnormal lipid metabolism in mice fed high fat diet (HFD). Fruit, root, stem, and leaf extracts of M. charantia were obtained using distilled water, 70% ethanol and 95% hexane. M. charantia leaf distilled water extract (MCLW) showed the highest antioxidant activity in both 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity tests and reducing power. Metabolite profiles of M. charantia leaf extracts were analyzed for identification of bioactive compounds. HFD-fed mice were treated with MCLW (oral dose of 200 mg/kg/d) for 4 weeks. MCLW reduced lipid accumulation, body weight, organ weight, and adipose tissue volume and significantly improved glucose tolerance and insulin resistance in HFD mice. Furthermore, MCLW administration reduced serum total cholesterol and low-density lipoprotein cholesterol, and increased serum high-density lipoprotein cholesterol compared with HFD mice. Moreover, MCLW significantly reduced the levels of serum urea nitrogen, alanine aminotransferase, alkaline phosphatase, and aspartate aminotransferase; alleviated liver and kidney injury. MCLW decreases expression of genes that fatty acid synthesis; increase the expression of catabolic-related genes. These results indicate that MCLW has an inhibitory effect on obese induced by high fat diet intake, and the mechanism may be related to the regulation of abnormal lipid metabolism in liver and adipose tissue, suggesting that MCLW may be a suitable candidate for the treatment of obesity.

scholarly journals Metronidazole enhances steatosis-related early-stage hepatocarcinogenesis in high fat diet-fed rats through DNA double-strand breaks and modulation of autophagy

2021 ◽  
Author(s):  
Ayumi Eguchi ◽  
Sayaka Mizukami ◽  
Misato Nakamura ◽  
Sousuke Masuda ◽  
Hirotada Murayama ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
High Fat Diet ◽  
Early Stage ◽  
Double Strand Breaks ◽  
Male Rats ◽  
Dna Double Strand Breaks ◽  
High Fat ◽  
Hepatic Disorder ◽  
Altered Expression ◽  
Strand Breaks

Abstract Nonalcoholic fatty liver disease is a hepatic disorder with deposition of fat droplets, and has a high risk of progression to steatosis-related hepatitis and irreversible hepatic cancer. Metronidazole (MNZ) is an antiprotozoal and antimicrobial agent widely used to treat patients infected with anaerobic bacteria and intestinal parasites; however, MNZ has also been shown to induce liver tumors in rodents. To investigate the effects of MNZ on steatosis-related early-stage hepatocarcinogenesis, male rats treated with N-nitrosodiethylamine following 2/3 hepatectomy at week 3 were received a control basal diet, high fat diet (HFD), or HFD containing 0.5% MNZ. The HFD induced obesity and steatosis in liver, accompanied by altered expression of Pparg and Fasn, genes related to lipid metabolism. MNZ increased nuclear translocation of lipid metabolism-related transcription factor peroxisome proliferator-activated receptor gamma in hepatocytes, together with altered liver expression of lipid metabolism genes (Srebf1, Srebf2, Pnpla2). Furthermore, MNZ significantly increased the number of preneoplastic liver foci, accompanied by DNA double-strand breaks and late-stage autophagy inhibition, as reflected by increased levels of γ-H2AX, LC3, and p62. Therefore, MNZ could induce steatosis-related hepatocarcinogenesis by inducing DNA double-strand breaks and modulating autophagy in HFD-fed rats.

scholarly journals Sex Differences in Gut Microbiome in High-Fat-Diet Fed Rats

2020 ◽  
Author(s):  
Ying Shi ◽  
Fangzhi Yue ◽  
Lin Xing ◽  
Shanyu Wu ◽  
Lin Wei ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Body Weight ◽  
Sex Differences ◽  
Gut Microbiota ◽  
High Fat Diet ◽  
Metabolic Diseases ◽  
Normal Diet ◽  
Female Rats ◽  
Male Rats ◽  
High Fat

Abstract Background: Sex differences in obesity and related metabolic diseases are well recognized, however, the mechanism has not been elucidated. Gut microbiota and its metabolites may play a vital role in the development of obesity and metabolic diseases. The aim of the present study was to investigate sex differences in gut microbiota and its metabolites in a high-fat-diet (HFD) obesity rats and identify microbiota genera potentially contributing to such differences in obesity and non-alcoholic fatty liver disease (NAFLD) susceptibility.Methods: Sprague–Dawley rats were divided into four groups (eight animals per group): (1) male rats on a normal diet (MND), (2) male rats on HFD (MHFD), (3) female rats on a normal diet (FND), and (4) female rats on HFD (FHFD). Body weight, liver pathology, gut microbiota and short/medium chain fatty acids in colon contents were compared between different sexes.Results: HFD induced more body weight gain and fat storage in female rats, however, lower hepatic steatosis in FHFD than in MHFD rats was observed. When considering gut microbiota composition, FHFD rats had lower microbiome diversity than MHFD. A significant increase of Firmicutes phylum, along with Bilophila and Blautia genus was detected in MHFD rats, as compared with FHFD, which showed increased relative abundance of Murimonas. Moreover, propionic and lauric acid levels were higher in FHFD than those in MHFD rats. Conclusions: HFD induced sex-related alterations in gut microbiome and fatty acids. Furthermore, the genus Bilophila, Blautia and Murimonas might contribute to sex differences observed in obesity and NAFLD susceptibility.

scholarly journals Pre and postnatal assessment of gross parameters after utilization of high fat diet in rats.

2021 ◽  
Vol 28 (04) ◽  
pp. 504-508
Author(s):  
Shahid Maqbool Korai ◽  
Hina Khan ◽  
Shahid Hussain Soomro ◽  
Muhammad Rafique ◽  
Raja Faisal ◽  
...  
Keyword(s):  
High Fat Diet ◽  
Normal Diet ◽  
Male Rats ◽  
Abdominal Circumference ◽  
Albino Rats ◽  
Maternal Weight ◽  
High Fat ◽  
Pregnant Rats ◽  
Randomized Sampling ◽  
Significant Difference

Objective: To assess the gross parameters of maternal weight and abdominal circumference upon utilization of high fat diet. Study Design: Experimental study. Setting: Al-Tibri Medical College and Hospital. Period: October 2019 to March 2020. Material & Methods: After taken an ethical approval total 16 female albino rats were selected and divided into two groups through randomized sampling and 2 male rats were introduced in each group for the purpose of mating, group A given a normal diet (control) and group B given a composition of high fat diet throughout the gestational period. The weights of the pregnant rats and the abdominal circumference were measured on day 1st and 21st of gestation. The data were analyzed through SPSS version 21.0, in the form of Mean ± standard deviation, and for the evaluation of level of significance independent “t” test was applied and considered value of significant was P=<0.05. Results: There was no significant difference in the weight or abdominal circumference of maternal rats in both groups. Conclusion: High fat diet having no significant effects on maternal weight and abdominal circumference as compared to normal diet. We need to assess other histomorphological parameters to assess the relative effects.

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