Biosynthesis of zinc oxide nanoparticles usingMangifera indica leaves and evaluation of their antioxidant and cytotoxic properties in lung cancer (A549) cells

2018 ◽  
Vol 117 ◽  
pp. 91-95 ◽  
Author(s):  
S. Rajeshkumar ◽  
S. Venkat Kumar ◽  
Arunachalam Ramaiah ◽  
Happy Agarwal ◽  
T. Lakshmi ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
A549 Cells ◽  
Oxide Nanoparticles

scholarly journals Investigation of Cytotoxicity Apoptotic and Inflammatory Responses of Biosynthesized Zinc Oxide Nanoparticles from Ocimum sanctum Linn in Human Skin Keratinocyte (Hacat) and Human Lung Epithelial (A549) Cells

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Bader Almutairi ◽  
Gadah Albahser ◽  
Rafa Almeer ◽  
Nouf M. Alyami ◽  
Hanouf Almukhlafi ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Human Skin ◽  
Zinc Oxide Nanoparticles ◽  
Human Lung ◽  
A549 Cells ◽  
Ocimum Sanctum ◽  
Oxide Nanoparticles ◽  
Dependent Manner ◽  
Hacat Cells ◽  
Lung Epithelial

Pristine and engineered metal nanoparticles are widely applied in various fields of industry, and as consequences, they are useful as well as harmful to human health and environment. This study aimed at synthesizing the green zinc oxide nanoparticles (ZnNPs) using the leaf extract of Ocimum sanctum Linn and assessing its toxicity on human skin epidermal (HaCaT) and human lung epithelial (A549) cells. The synthesized green ZnNPs (gZnNPs) were characterized by using dynamic light scattering (DLS) and a high-resolution transmission electron microscope. The average size of gZnNPs obtained was 62 nm with a spherical shape. The effects of gZnNPs on the viability of HaCaT and A549 cells were investigated using tetrazolium salt (MTT) for 24 h. We have seen more reduction of cell viability of A549 cells in comparison to HaCaT cells. The induction of intracellular reactive oxygen species (ROS) was measured using DCFDA assay and showed a slightly high intensity of green fluorescence in A549 than HaCaT cells. The different oxidative stress biomarkers such as ROS generation and lipid peroxide were increased, and GSH was decreased in a dose-dependent manner. The apoptotic and necrotic effect of gZnNPs in both cells was carried out using Annexin-V-FITC and propidium iodide staining. More apoptotic and necrotic cells were found at a higher concentration of gZnNPs exposure. Also, we determined the effect of gZnNPs at the molecular level by evaluating the apoptotic and inflammatory markers, in which gZnNPs downregulated Bcl2 and upregulated Bax, caspase-3, and TNF-α in HaCaT and A549 cells. Ultimately, gZnNPs exerted toxicity and apoptosis in HaCaT and A549 cells.

scholarly journals Protein Profile of Human Lung Epithelial Cells (A549) Revealing Deviation in Cytoskeleton Proteins in Response to Zinc Oxide Nanoparticles Exposure

2020 ◽  
Vol 5 (3) ◽  
pp. 163-172
Author(s):  
Avnika Singh Anand ◽  
Khushbu Jain ◽  
Rahul Ranjan ◽  
Dipti N. Prasad ◽  
Amitabh Chauhan ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Protein Profile ◽  
A549 Cells ◽  
Lung Epithelial Cells ◽  
Oxide Nanoparticles ◽  
Cellular Interactions ◽  
Proteomics Data ◽  
Zno Nps ◽  
Ontology Term

Zinc oxide nanoparticles (ZnO NPs) are widely used in biomedicine and scientific research because of their high dissolution property and bioavailability. On the contrary, this property also increases the intracellular reactivity, accessibility and cytotoxicity. These nano-bio interactions could induce undesirable changes in the proteome of the interacting cells, especially in the lung cells as these are the primary contact site. However, the potential effects of ZnO NPs exposure on proteome remain unclear. Proteomics data will substantiate the detailed mechanism of cellular interactions and modulatory effects of ZnO NPs on cells. Quantitative proteomic profiling was done using MALDI-TOF/TOF and MS/MS to identify differential protein expression on exposure to NPs among non exposed and exposed cells. Twenty-two proteins, with approximately 1.5 fold differential expression in cells exposed to ZnO NPs as compared to control cells were identified. Differentially expressed proteins were further classified using PANTHER software on the basis of functional gene ontology term: molecular function, biological process and cellular component. ToppGene suite was used to study protein-protein interaction and network was enriched with STRING. This study is a systematic analysis of protein modulation of the A549 cells exposed to ZnO NPs indicating alterations in the cytoskeleton.

scholarly journals Multi-omics approaches confirm metal ions mediate the main toxicological pathways of metal-bearing nanoparticles in lung epithelial A549 cells

2018 ◽  
Vol 5 (6) ◽  
pp. 1506-1517 ◽  
Author(s):  
Susan Dekkers ◽  
Tim D. Williams ◽  
Jinkang Zhang ◽  
Jiarui (Albert) Zhou ◽  
Rob J. Vandebriel ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Metal Ions ◽  
Zinc Oxide Nanoparticles ◽  
A549 Cells ◽  
Oxide Nanoparticles ◽  
Lung Epithelial

Our analyses confirm that the dissolution of metal ions mediates the main toxicological pathways of silver and zinc oxide nanoparticles.

Optical Characteristics of Zinc Oxide Nanoparticles with Surface Modification Using Polyethylene Glycol

2014 ◽  
Vol 29 (10) ◽  
pp. 1039
Author(s):  
TAN Man-Lin ◽  
WANG Yan-Tao ◽  
ZHANG Wei-Li ◽  
FU Dong-Ju ◽  
LI Dong-Shuang ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Surface Modification ◽  
Polyethylene Glycol ◽  
Zinc Oxide Nanoparticles ◽  
Optical Characteristics ◽  
Oxide Nanoparticles

A comparative study of the antibacterial activity of Quercus robur (Ethanolic extract) and Zinc oxide nanoparticles on Salmonella (In vitro)

2018 ◽  
Vol 9 (SPL1) ◽  
Author(s):  
Hams H. H. Alfattli ◽  
Ghufran Zuhair Jiber ◽  
Ghaidaa Gatea Abbass
Keyword(s):  
Zinc Oxide ◽  
Antibacterial Activity ◽  
Zinc Oxide Nanoparticles ◽  
Quercus Robur ◽  
Ethanolic Extract ◽  
Inhibitory Effect ◽  
Significant Inhibition ◽  
Oxide Nanoparticles ◽  
Inhibition Zones

This study which designed to evaluate the inhibitory effect of Ethanolic extract of (Quercusrobur) and Zinc oxide nanoparticles on the growth of one genus of enterobacteriacae (Salmonella). In vitro. For this purpose graduate concentrates for plant extract (50, 100, 200, 400 )mg/ml which prepared and compared with Zinc oxide nanoparticles of different concentration (2, 1, 0.5, 0.25) μg/ml,and examined. The result showed that the studied medicinal plant has antibacterial activity against this bacteria which used. The result showed that the plant has good activity in decrease the growth of this bacteria. The results of the study also showed that the nano-ZnO has very effective antibacterial action against the studied bacteria which was Salmonella,nanoparticles concentrations lead to increasing in the inhibition zones of tested bacterial growth. We also study the effect of three antibiotics Lomefloxacin (LOM), Ciprofloxacin (SIP) and Rifampin (RA) and the result showed,in a comparison within the tested bacteria,Salmonella had a significant inhibition increase in Lomefloxacin ; the ciprofloxacin showed effect on tested bacteria. However,Rifampin does not show any effect on tested bacteria.

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