18β-Glycyrrhetinic acid, the major bioactive component of Glycyrrhizae Radix, attenuates airway inflammation by modulating Th2 cytokines, GATA-3, STAT6, and Foxp3 transcription factors in an asthmatic mouse model

2017 ◽  
Vol 52 ◽  
pp. 99-113 ◽  
Author(s):  
Seung-Hyung Kim ◽  
Jung-hee Hong ◽  
Ji-Eun Lee ◽  
Young-Cheol Lee
Keyword(s):  
Transcription Factors ◽  
Mouse Model ◽  
Airway Inflammation ◽  
Glycyrrhetinic Acid ◽  
Th2 Cytokines ◽  
Glycyrrhizae Radix ◽  
Bioactive Component

scholarly journals Justicia procumbens Extract (DW2008) Selectively Suppresses Th2 Cytokines in Splenocytes and Ameliorates Ovalbumin-Induced Airway Inflammation in a Mouse Model of Asthma

2017 ◽  
Vol 40 (9) ◽  
pp. 1416-1422 ◽  
Author(s):  
Jihyun Youm ◽  
Hyunyong Lee ◽  
Hwan Bong Chang ◽  
Jihyun Jeon ◽  
Mi Hee Yoon ◽  
...  
Keyword(s):  
Mouse Model ◽  
Airway Inflammation ◽  
Th2 Cytokines

Carica papaya Leaf Extract modulates mRNA expression of Aquaporins in Mouse Model of Allergic Airway Inflammation

2021 ◽  
Vol 15 (10) ◽  
pp. 2512-2515
Author(s):  
Asma Inam ◽  
Muhammad Sair ◽  
Sadia Ikram ◽  
Sadia Majeed ◽  
Gul-E- Nazish ◽  
...  
Keyword(s):  
Body Weight ◽  
Mouse Model ◽  
Pulmonary Edema ◽  
Mrna Expression ◽  
Airway Inflammation ◽  
Allergic Asthma ◽  
Leaf Extract ◽  
Carica Papaya ◽  
Allergic Airway Inflammation ◽  
Th2 Cytokines

Background: Asthma is a chronic inflammatory disease affecting smaller airways. Airflow obstruction leading to airway hyper-responsiveness and increased mucus production are salient features of asthma pathophysiology. Generally, Th2 cytokines are increased in allergic asthma. Aim: To propose the molecular mechanisms by which Carica Papaya Leaves Extract (CPLE) alleviates pulmonary edema in animal model of allergic airway inflammation comparable to methylprednisolone. Place and duration of study: Pharmacology Department, University of Health Sciences Lahore for 1 year. Methods: We took twenty four male BALB/c mice and divided them equally into four groups. The control group was given PBS only, while Group II served as diseased group and induced airway inflammation by ovalbumin. Group III and IV were first induced with airway inflammation and side by side treated with Carica papaya leaf extract (CPLE) 100mg/kg body weight orally and methylprednisolone 15 mg/kg body weight intraperitoneally for seven consecutive days respectively. At the end of the experimental protocol, mice were euthanized and lung wet/dry ratio was measured. mRNA expression of AQP1 and AQP5 in lung tissue were also determined using RT-PCR. Results: Ethanolic extract of Carica Papaya leaves decreased all markers of pulmonary edema in mouse model of allergic airway inflammation comparable to methylprednisolone by decreasing lung wet/dry ratio and enhancing AQP1 and AQP5 mRNA expression. Conclusion: Carica Papaya leaves extract may diminish pulmonary edema in mice associated with allergic asthma. Keywords: AQP1, AQP5 (Aquaporins), Carica Papaya Leaves Extract (CPLE), Pulmonary Edema, Th2 cytokines.

scholarly journals Antiasthmatic Effects of Hesperidin, a Potential Th2 Cytokine Antagonist, in a Mouse Model of Allergic Asthma

2011 ◽  
Vol 2011 ◽  
pp. 1-12 ◽  
Author(s):  
Seung-Hyung Kim ◽  
Bok-Kyu Kim ◽  
Young-Cheol Lee
Keyword(s):  
Mouse Model ◽  
Airway Inflammation ◽  
Airway Hyperresponsiveness ◽  
Immune Cell ◽  
Airflow Obstruction ◽  
Specific Ige ◽  
Eosinophil Infiltration ◽  
Th2 Cytokines ◽  
Th2 Cytokine

Background and Objective. The features of asthma are airway inflammation, reversible airflow obstruction, and an increased sensitivity to bronchoconstricting agents, termed airway hyperresponsiveness (AHR), excess production of Th2 cytokines, and eosinophil accumulation in the lungs. To investigate the antiasthmatic potential of hesperidin as well as the underlying mechanism involved, we studied the inhibitory effect and anti-inflammatory effect of hesperidin (HPN) on the production of Th2 cytokines, eotaxin, IL-17, -OVA-specific IgEin vivoasthma model mice.Methods. In this paper, BALB/c mice were systemically sensitized to ovalbumin (OVA) followed intratracheally, intraperitoneally, and by aerosol allergen challenges. We investigated the effect of HPN on airway hyperresponsiveness, pulmonary eosinophilic infiltration, various immune cell phenotypes, Th2 cytokine production and OVA-specific IgE production in a mouse model of asthma.Results. In BALB/c mice, we found that HPN-treated groups had suppressed eosinophil infiltration, allergic airway inflammation, and AHR, and these occurred by suppressing the production of IL-5, IL-17, and OVA-specific IgE.Conclusions. Our data suggest that the therapeutic mechanism by which HPN effectively treats asthma is based on reductions of Th2 cytokines (IL-5), eotaxin, OVA-specific IgE production, and eosinophil infiltration via inhibition of GATA-3 transcription factor.

scholarly journals Sinomenine attenuates airway inflammation and remodeling in a mouse model of asthma

2016 ◽  
Vol 13 (3) ◽  
pp. 2415-2422 ◽  
Author(s):  
HAI-RONG BAO ◽  
XIAO-JU LIU ◽  
YUN-LIN LI ◽  
XIANG MEN ◽  
XIAO-LI ZENG
Keyword(s):  
Mouse Model ◽  
Airway Inflammation

Astragalus membranaceus modulates Th1/2 immune balance and activates PPARγ in a murine asthma model

2014 ◽  
Vol 92 (5) ◽  
pp. 397-405 ◽  
Author(s):  
Shih-Ming Chen ◽  
Yau-Sheng Tsai ◽  
Su-Wen Lee ◽  
Ya-Hui Liu ◽  
Shuen-Kuei Liao ◽  
...  
Keyword(s):  
Airway Inflammation ◽  
Chinese Herb ◽  
A549 Cells ◽  
Nuclear Hormone Receptor ◽  
Astragalus Membranaceus ◽  
Luciferase Reporter ◽  
Mrna Levels ◽  
Th2 Cytokines ◽  
Asthma Model ◽  
Murine Asthma Model

Astragalus membranaceus, a traditional Chinese herb, has been used to improve airway inflammation and asthma. The present study investigated whether A. membranaceus has immunotherapeutic effects on asthma, a chronic inflammatory mucosal disease that is associated with excess production of IgE, eosinophilia, T helper 2 (Th2) cytokines, and bronchial hyperresponsiveness. An ovalbumin (OVA)-induced, chronic inflammatory airway murine asthma model was used to examine the status of pulmonary inflammation after the administration of A. membranaceus. The IgE levels in serum and bronchoalveolar lavage fluid showed a tendency to decrease after the administration of A. membranaceus. The number of eosinophils decreased and infiltration of inflammatory cells and collagen deposition declined in lung sections after A. membranaceus administration. The RNA and protein levels of Th2 cytokines and the ratio of the GATA3/T-bet mRNA levels decreased after A. membranaceus treatment. Furthermore, the mRNA level of peroxisome proliferator-activated receptor γ (PPARγ), a nuclear hormone receptor, increased in the lung tissues of A. membranaceus–treated mice. Finally, an A. membranaceus water extract activated PPARγ activity in either human embryonic kidney 293 (HEK293) or A549 cells in a PPARγ-responsive element-containing luciferase reporter assay. These results indicate that A. membranaceus has an inhibitory effect on airway inflammation in a murine model of asthma through modulating the imbalanced relationship between Th1 and Th2 cytokines.

The protective effect of solidagenone from Solidago chilensis Meyen in a mouse model of airway inflammation

2021 ◽  
Author(s):  
Juliana Fraga Vasconcelos ◽  
Ivanilson Pimenta Santos ◽  
Temistocles Barroso Oliveira ◽  
Andressa Maia Kelly ◽  
Bruna Padilha Zurita Claro do Reis ◽  
...  
Keyword(s):  
Mouse Model ◽  
Protective Effect ◽  
Airway Inflammation ◽  
Solidago Chilensis
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