Methoxy VO-salen complex: In vitro antioxidant activity, cytotoxicity evaluation and protective effect on CCl4-induced oxidative stress in rats

AbstractThe current study investigated the protective effect of Hertia cheirifolia L. n-butanol extract on oxidative stress in vitro by measuring lipid peroxidation (MDA) level and superoxide anion (O2•-) production in liver and heart mitochondria of rat. In addition, the antioxidant potential of H. cheirifolia n-butanol extract was evaluated by using five methods: ABTS•+, O2•-, Bleaching of β-carotene in linoleic acid, CUPRAC and Ferric reducing power. The results indicated that n-butanol extract contained large amounts of total phenolics (203.52±1.81 mg GAE/g) and flavonoids (104.86±0.57 mg QE/g), and had an interesting antioxidant activity and protective effect on mitochondrial oxidative stress. Therefore, H. cheirifolia n-butanol extract may serve as potential source of natural antioxidant for pharmaceutical applications.

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In vitro antioxidant activity, α-glucosidase inhibitory potential and in vivo protective effect of Asparagus stipularis Forssk aqueous extract against high-fructose diet-induced metabolic syndrome in rats

Journal of Functional Foods ◽

10.1016/j.jff.2018.06.006 ◽

2018 ◽

Vol 47 ◽

pp. 521-530 ◽

Cited By ~ 2

Author(s):

Khaoula Adouni ◽

Olfa Zouaoui ◽

Hassiba Chahdoura ◽

Amira Thouri ◽

Jihen Ben Lamine ◽

...

Keyword(s):

Metabolic Syndrome ◽

Antioxidant Activity ◽

Protective Effect ◽

Aqueous Extract ◽

High Fructose Diet ◽

High Fructose ◽

Inhibitory Potential ◽

In Vitro Antioxidant Activity

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In-vitro and In-vivo Antioxidant Activity of Ethanol Leaf Extract of Justicia carnea

International Journal of Biochemistry Research & Review ◽

10.9734/ijbcrr/2020/v29i430185 ◽

2020 ◽

pp. 48-60

Author(s):

Udedi Stanley Chidi ◽

Ani Onuabuchi Nnenna ◽

Asogwa Kingsley Kelechi ◽

Maduji Fitzcharles Chijindu ◽

Okafor Clinton Nebolisa

Keyword(s):

Oxidative Stress ◽

Lipid Peroxidation ◽

Antioxidant Activity ◽

Ascorbic Acid ◽

Superoxide Dismutase ◽

Leaf Extract ◽

Dpph Radical ◽

Β Carotene ◽

In Vitro Antioxidant Activity

This study investigated the in-vitro antioxidant activity of ethanol leaf extract of Justicia carnea and its effect on antioxidant status of alloxan-induced diabetic albino rats. The in-vitro antioxidant activity was assayed by determining the total phenol, flavonoids, ascorbic acid, β-carotene and lycopene contents and by using 2,2 diphenyl-1-picrylhydrazyl (DPPH) radical, reducing antioxidant power and inhibition of lipid peroxidation antioxidant systems. Oxidative stress was produced in rats by single intraperitoneal injection of 150 mg/kg alloxan and serum concentration of malonaldehyde (MDA), superoxide dismutase (SOD) and catalase (CAT) were determined. Five experimental groups of rats (n=6) were used for the study. Two groups of diabetic rats received oral daily doses of 100 and 200 mg/kg Justicia carnea leaf extract respectively while gilbenclamide (5 mg/ml); a standard diabetic drug was also given to a specific group for 14 days. From the result, the leaf extract contained a higher concentration of flavonoids followed byphenols, ascorbic acid, lycopene and β-carotene. The extract displayed more potent reducing power ability with EC50 of 40 µg/ml compared to BHA (EC50 of 400µg/ml). The percentage DPPH radical scavenging activity of the extract was also higher with EC50 of 200µg/ml and increased with increase in concentration while BHA had EC50of 320µg/ml. The inhibition of lipid peroxidation also increased with increase in concentration with EC50 of 58µg/ml and comparable with BHA (EC50=60µg/ml). The effect of the plant extract on antioxidant enzyme activities was concentration-dependent. Administration of 100mg/kg of the plant extract resulted in a significant decrease (p<0.05) in serum MDA concentration, while 200 mg/kg of the extract caused a significant (p˂0.05) increase in superoxide dismutase (SOD) and catalase activities with a non-significant increase (p>0.05) in the serum level of MDA when compared with the diabetic untreated group. These findings suggest that ethanol leaf extract of Justicia carnea have antioxidant properties and could handle diabetes-induced oxidative stress.

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Metabolomic Profiling of Mango (Mangifera indica Linn) Leaf Extract and Its Intestinal Protective Effect and Antioxidant Activity in Different Biological Models

Molecules ◽

10.3390/molecules25215149 ◽

2020 ◽

Vol 25 (21) ◽

pp. 5149

Author(s):

Roberto O. Ybañez-Julca ◽

Daniel Asunción-Alvarez ◽

Ivan M. Quispe-Díaz ◽

Javier Palacios ◽

Jorge Bórquez ◽

...

Keyword(s):

Oxidative Stress ◽

Antioxidant Activity ◽

Protective Effect ◽

High Performance ◽

Mangifera Indica ◽

Folk Medicine ◽

In Vitro Assays ◽

Antispasmodic Activity ◽

Intestinal Segments

Mangifera indica Linn popularly known as mango is used in folk medicine to treat gastrointestinal disorders. The aim of this study was to identify the metabolomic composition of lyophilized extract of mango leaf (MIE), to evaluate the antioxidant activity on several oxidative stress systems (DPPH, FRAP, TBARS, and ABTS), the spasmolytic and antispasmodic activity, and intestinal protective effect on oxidative stress induced by H2O2 in rat ileum. Twenty-nine metabolites were identified and characterized based on their ultra-high-performance liquid chromatography (UHPLC) high-resolution orbitrap mass spectrometry, these include: benzophenone derivatives, xanthones, phenolic acids, fatty acids, flavonoids and procyanidins. Extract demonstrated a high antioxidant activity in in-vitro assays. MIE relaxed (p < 0.001) intestinal segments of rat pre-contracted with acetylcholine (ACh) (10−5 M). Pre-incubation of intestinal segments with 100 µg/mL MIE significantly reduced (p < 0.001) the contraction to H2O2. Similar effects were observed with mangiferin and quercetin (10−5 M; p < 0.05) but not for gallic acid. Chronic treatment of rats with MIE (50 mg/kg) for 28 days significantly reduced (p < 0.001) the H2O2-induced contractions. MIE exhibited a strong antioxidant activity, spasmolytic and antispasmodic activity, which could contribute to its use as an alternative for the management of several intestinal diseases related to oxidative stress.

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In Vitro Antioxidant Activity of Feronia Limonia Bark Relevant to the Treatment of Oxidative Stress Mediated Neurodegenerative Disorders

World Scientific Research ◽

10.20448/journal.510/2016.3.1/510.1.62.69 ◽

2016 ◽

Vol 3 (1) ◽

pp. 62-69

Author(s):

Abida Akter ◽

◽

Md. Golam Sadik ◽

Md. Abdul Kader ◽

Km Monirul Islam ◽

...

Keyword(s):

Oxidative Stress ◽

Antioxidant Activity ◽

Neurodegenerative Disorders ◽

In Vitro Antioxidant Activity

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Topical Formulations Containing Pimenta pseudocaryophyllus Extract: In Vitro Antioxidant Activity and In Vivo Efficacy Against UV-B-Induced Oxidative Stress

AAPS PharmSciTech ◽

10.1208/s12249-013-0049-8 ◽

2013 ◽

Vol 15 (1) ◽

pp. 86-95 ◽

Cited By ~ 18

Author(s):

Marcela Z. Campanini ◽

Dayana L. Custódio ◽

Ana L. M. Ivan ◽

Sarah M. Martins ◽

Maria J. R. Paranzini ◽

...

Keyword(s):

Oxidative Stress ◽

Antioxidant Activity ◽

In Vivo Efficacy ◽

Topical Formulations ◽

In Vitro Antioxidant Activity

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IN VIVO ANTIOXIDANT ACTIVITY OF PHYLLANTHUS EMBLICUS AGAINST CISPLATIN INDUCED OXIDATIVE STRESS IN MICE

Journal of Applied Pharmacy ◽

10.21065/19204159.8.01 ◽

2016 ◽

Vol 8 ◽

pp. 1

Author(s):

Maimuna Naaz ◽

Keyword(s):

Oxidative Stress ◽

Antioxidant Activity ◽

Free Radicals ◽

The Body ◽

Cytotoxic Drug ◽

Rich Source ◽

In Vitro Antioxidant Activity

Plants are rich source of antioxidants. They can ameliorate the oxidative stress induced complications. Cisplatin is a cytotoxic drug which produced unwanted effects in the patients due to generation of free radicals inside the body. Phyllanthus emblicuspossessed in vitro antioxidant activity.

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