Gestational exposure to acrylamide suppresses luteal endocrine function through dysregulation of ovarian angiogenesis, oxidative stress and apoptosis in mice

ABSTRACT Background It is unknown which compounds in spermatozoa or seminal plasma may be involved in the regulation of sperm motility. Objectives The aim of this study was to investigate the effects of DHA (22:6n–3), vitamin E, and their probable interactions in men with asthenozoospermia. Methods A factorial, randomized, double-blind, placebo-controlled trial was conducted in infertility clinics in Tehran, Iran. The participants were idiopathic asthenozoospermic men aged 20–45 y, with normal endocrine function. Their concentration of spermatozoa and percentage of morphologically normal spermatozoa were equal to or above the lower reference limits, according to the fifth edition of the WHO guideline. Out of 717 men referred to the infertility clinics, 180 asthenozoospermic men were randomly assigned to 1 of 4 groups according to stratified blocked randomization by age and sperm concentration. Participants took daily 465 mg DHA plus 600 IU vitamin E (DE), 465 mg DHA plus placebo (DP), 600 IU vitamin E plus placebo (EP), or both placebo capsules (PP) for 12 wk. Sperm characteristics, oxidative stress of seminal plasma, serum and sperm membrane fatty acids, dietary intakes, anthropometric measurements, and physical activity were measured at baseline and after 12 wk. Results After the intervention, mean ± SD sperm progressive motility was greater in the DE group (27.9 ± 2.8) than in the DP (25.7 ± 3.4), EP (26.1 ± 2.8), and PP (25.8 ± 2.6) groups (P < 0.05). Sperm count (P = 0.001) and concentration (P = 0.044) increased significantly in the DE group compared with the other 3 groups, whereas other semen parameters were not significantly different between the groups after the intervention. Serum concentrations of n–3 PUFAs were significantly higher in the DE and DP groups than in the EP and PP groups. Conclusions Combined DHA and vitamin E supplements led to increased sperm motility; however, no significant changes occurred in sperm morphology and vitality in asthenozoospermic men. This trial was registered at clinicaltrials.gov as NCT01846325.

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Alterations in Energy Metabolism, Mitochondrial Function and Redox Homeostasis in GK Diabetic Rat Tissues Treated with Aspirin

Life ◽

10.3390/life12010104 ◽

2022 ◽

Vol 12 (1) ◽

pp. 104

Author(s):

Annie John ◽

Layla Amiri ◽

Jasmin Shafarin ◽

Saeed Tariq ◽

Ernest Adeghate ◽

...

Keyword(s):

Oxidative Stress ◽

Energy Metabolism ◽

Mitochondrial Function ◽

Redox Homeostasis ◽

Diabetic Rats ◽

Endocrine Function ◽

Diabetic Rat ◽

Rat Tissues ◽

Gk Rats ◽

And Oxidative Stress

Our recent studies have demonstrated that aspirin treatment prevents inflammatory and oxidative stress-induced alterations in mitochondrial function, improves glucose tolerance and pancreatic endocrine function and preserves tissue-specific glutathione (GSH)-dependent redox homeostasis in Goto-Kakizaki (GK) diabetic rats. In the current study, we have investigated the mechanism of action of aspirin in maintaining mitochondrial bioenergetics and redox metabolism in the liver and kidneys of GK rats. Aspirin reduced the production of reactive oxygen species (ROS) and oxidative stress-induced changes in GSH metabolism. Aspirin treatment also improved mitochondrial respiratory function and energy metabolism, in addition to regulating the expression of cell signaling proteins that were altered in diabetic animals. Ultrastructural electron microscopy studies revealed decreased accumulation of glycogen in the liver of aspirin-treated diabetic rats. Hypertrophic podocytes with irregular fusion of foot processes in the renal glomerulus and detached microvilli, condensed nuclei and degenerated mitochondria observed in the proximal convoluted tubules of GK rats were partially restored by aspirin. These results provide additional evidence to support our previous observation of moderation of diabetic complications by aspirin treatment in GK rats and may have implications for cautious use of aspirin in the therapeutic management of diabetes.

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Islet NADPH oxidase activity modulates β-cell mass and endocrine function in rats with fructose-induced oxidative stress

Biochimica et Biophysica Acta (BBA) - General Subjects ◽

10.1016/j.bbagen.2014.09.011 ◽

2014 ◽

Vol 1840 (12) ◽

pp. 3475-3482 ◽

Cited By ~ 7

Author(s):

Carolina L. Román ◽

Luis E. Flores ◽

Bárbara Maiztegui ◽

María A. Raschia ◽

Héctor Del Zotto ◽

...

Keyword(s):

Oxidative Stress ◽

Nadph Oxidase ◽

Oxidase Activity ◽

Cell Mass ◽

Endocrine Function ◽

Β Cell ◽

Nadph Oxidase Activity

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Fucoidan Hydrogels Significantly Alleviate Oxidative Stress and Enhance the Endocrine Function of Encapsulated Beta Cells

Advanced Functional Materials ◽

10.1002/adfm.202011205 ◽

2021 ◽

pp. 2011205

Author(s):

Lara L. Reys ◽

Vijayaganapathy Vaithilingam ◽

Mireille M. J. P. E. Sthijns ◽

Eduardo Soares ◽

Timo Rademakers ◽

...

Keyword(s):

Oxidative Stress ◽

Beta Cells ◽

Endocrine Function

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Impact of a gestational exposure to diesel exhaust on offspring gonadal development: experimental study in the rabbit

Journal of Developmental Origins of Health and Disease ◽

10.1017/s2040174418000351 ◽

2018 ◽

Vol 9 (5) ◽

pp. 519-529 ◽

Cited By ~ 5

Author(s):

M. Bourdon ◽

L. Torres-Rovira ◽

D. Monniaux ◽

C. Faure ◽

R. Levy ◽

...

Keyword(s):

Dna Fragmentation ◽

Diesel Exhaust ◽

Rabbit Model ◽

Gonadal Development ◽

Endocrine Function ◽

Sperm Dna Fragmentation ◽

Gestational Exposure ◽

Sperm Dna ◽

And Function ◽

And Control

AbstractThe aim of the present work was to address experimentally the possible impact of exposure to air pollution during gestation on the differentiation and function of the gonads of the offspring using a rabbit model. Rabbits were exposed daily to diluted diesel exhaust gas or filtered air from the 3rd until the 27th day of gestation, during which time germ cells migrate in genital ridges and divide, and fetal sex is determined. Offspring gonads were collected shortly before birth (28th day of gestation) or after puberty (7.5 months after birth). The structure of the gonads was analyzed by histological and immunohistological methods. Serum concentrations of testosterone and anti-Müllerian hormone were determined using ELISA. The morphology and the endocrine function of the gonads collected just at the arrest of the exposure were similar in polluted and control animals in both sexes. No differences were observed as well in gonads collected after puberty. Sperm was collected at the head of the epididymis in adults. Sperm motility and DNA fragmentation were measured. Among all parameters analyzed, only the sperm DNA fragmentation rate was increased three-fold in exposed males. Mechanisms responsible for these modifications and their physiological consequences are to be further clarified.

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The peculiarities of hormonal background in boars under correction of reproductive capacity by gadolinium orthovanadate nanoparticles

Scientific Messenger of LNU of Veterinary Medicine and Biotechnology ◽

10.32718/nvlvet10411 ◽

2021 ◽

Vol 23 (104) ◽

pp. 66-70

Author(s):

V. I. Koshevoy ◽

S. V. Naumenko ◽

V. K. Klochkov ◽

S. L. Yefimova

Keyword(s):

Oxidative Stress ◽

Rare Earth ◽

Rare Earth Elements ◽

The Body ◽

Endocrine Function ◽

Reproductive Capacity ◽

Necessary Condition ◽

Control Group ◽

Total Testosterone ◽

Positive Effect

Determination of the influence of redox nanomaterials on the body of males is of great interest to researchers, because the basis of the pathogenesis of male infertility is oxidative stress. A necessary condition in the development of such drugs is the absence of toxic effects on sexual function. It has been proved that nanomaterials based on oxides of rare earth elements have a positive effect on reproductive capacity and, at the same time, they are not gonadotoxic. A comprehensive approach is of particular importance in order to justify their use as a means of reproductive capacity correction. Taking into account the toxicity parameters the mechanisms of their action need to be studied at the biochemical, morphological and clinical levels. The positive effect of gadolinium orthovanadate nanoparticles on the quality of boar sperm (especially in terms of motility and the number of motile sperm in the ejaculate) with an effective reduction of the oxidative load on the body have been shown. The aim of this study was to determine the effect of these nanoparticles on the hormonal background of males with a decreased reproductive capacity under oxidative stress. Thus, the administration of hydrosol of gadolinium orthovanadate nanoparticles for 14 days revealed a normalization of sex hormones – an increase in total testosterone, in particular, on the 15th day of the study – by 22.6 % (P ˂ 0.01), and on the 30th day – by 77.4 % (P ˂ 0.001) compared with the group of animals before the administration. There was a decrease in the level of 17β-estradiol on the 30th day – by 25.0 % (P ˂ 0.01), which almost reached the values of the control group. A decrease in the content of globulin testosterone-estradiol-binding in boars of the experimental group, in particular, on the 15th day of the experiment – by 13.0 % (P ˂ 0.05), and on the 30th day – by 26.8 % (P ˂ 0.001) was determined, which, in turn, led to an increase in androgen saturation of the animal body – the index of free androgens at the end of the study was 43.2 %. The results are explained by the properties of the nanoparticles. Correcting oxidative stress, they increase the antioxidant potential, thereby normalizing the activity of endocrine glands and ways of regulating the germ-endocrine function of the gonads. The prospect of further research is to elucidate the effect of the correction of decreased reproductive capacity in boars under oxidative stress by nanoparticles of oxides of rare earth elements on the morphological state of the gonads.

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Fucoidan Hydrogels Significantly Alleviate Oxidative Stress and Enhance the Endocrine Function of Encapsulated Beta Cells (Adv. Funct. Mater. 35/2021)

Advanced Functional Materials ◽

10.1002/adfm.202170255 ◽

2021 ◽

Vol 31 (35) ◽

pp. 2170255

Author(s):

Lara L. Reys ◽

Vijayaganapathy Vaithilingam ◽

Mireille M. J. P. E. Sthijns ◽

Eduardo Soares ◽

Timo Rademakers ◽

...

Keyword(s):

Oxidative Stress ◽

Beta Cells ◽

Endocrine Function

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Gestational Exposure to Pesticides Induces Oxidative Stress and Lipid Peroxidation in Offspring that Persist at Adult Age in an Animal Model

Toxicological Research ◽

10.5487/tr.2019.35.3.241 ◽

2019 ◽

Vol 35 (3) ◽

pp. 241-248 ◽

Cited By ~ 1

Author(s):

Elvis Ngwa Ndonwi ◽

Barbara Atogho-Tiedeu ◽

Eric Lontchi-Yimagou ◽

Tijjani S. Shinkafi ◽

Dieudonne Nanfa ◽

...

Keyword(s):

Oxidative Stress ◽

Lipid Peroxidation ◽

Animal Model ◽

Adult Age ◽

Gestational Exposure

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Lycopene alleviates sulfamethoxazole-induced hepatotoxicity in grass carp (Ctenopharyngodon idellus) via suppression of oxidative stress, inflammation and apoptosis

Food & Function ◽

10.1039/d0fo01638a ◽

2020 ◽

Vol 11 (10) ◽

pp. 8547-8559

Author(s):

Hongjing Zhao ◽

Yu Wang ◽

Mengyao Mu ◽

Menghao Guo ◽

Hongxian Yu ◽

...

Keyword(s):

Oxidative Stress ◽

Secondary Metabolites ◽

Human Health ◽

Grass Carp ◽

Aquatic Environment ◽

Ctenopharyngodon Idellus

Antibiotics are used worldwide to treat diseases in humans and other animals; most of them and their secondary metabolites are discharged into the aquatic environment, posing a serious threat to human health.

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Copper-dependent ATP7B up-regulation drives the resistance of TMEM16A-overexpressing head-and-neck cancer models to platinum toxicity

Biochemical Journal ◽

10.1042/bcj20190591 ◽

2019 ◽

Vol 476 (24) ◽

pp. 3705-3719 ◽

Cited By ~ 2

Author(s):

Avani Vyas ◽

Umamaheswar Duvvuri ◽

Kirill Kiselyov

Keyword(s):

Oxidative Stress ◽

Head And Neck ◽

Transcriptional Activation ◽

Platinum Resistance ◽

Mrna Levels ◽

Strong Positive Correlation ◽

Platinum Compounds ◽

Copper Chelation ◽

Novel Approach ◽

Cancer Models

Platinum-containing drugs such as cisplatin and carboplatin are routinely used for the treatment of many solid tumors including squamous cell carcinoma of the head and neck (SCCHN). However, SCCHN resistance to platinum compounds is well documented. The resistance to platinum has been linked to the activity of divalent transporter ATP7B, which pumps platinum from the cytoplasm into lysosomes, decreasing its concentration in the cytoplasm. Several cancer models show increased expression of ATP7B; however, the reason for such an increase is not known. Here we show a strong positive correlation between mRNA levels of TMEM16A and ATP7B in human SCCHN tumors. TMEM16A overexpression and depletion in SCCHN cell lines caused parallel changes in the ATP7B mRNA levels. The ATP7B increase in TMEM16A-overexpressing cells was reversed by suppression of NADPH oxidase 2 (NOX2), by the antioxidant N-Acetyl-Cysteine (NAC) and by copper chelation using cuprizone and bathocuproine sulphonate (BCS). Pretreatment with either chelator significantly increased cisplatin's sensitivity, particularly in the context of TMEM16A overexpression. We propose that increased oxidative stress in TMEM16A-overexpressing cells liberates the chelated copper in the cytoplasm, leading to the transcriptional activation of ATP7B expression. This, in turn, decreases the efficacy of platinum compounds by promoting their vesicular sequestration. We think that such a new explanation of the mechanism of SCCHN tumors’ platinum resistance identifies novel approach to treating these tumors.

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