Chronic exposure to a low concentration of bisphenol A during follicle culture affects the epigenetic status of germinal vesicles and metaphase II oocytes

2013 ◽  
Vol 100 (6) ◽  
pp. 1758-1767.e1 ◽  
Author(s):  
Tom Trapphoff ◽  
Martyna Heiligentag ◽  
Nady El Hajj ◽  
Thomas Haaf ◽  
Ursula Eichenlaub-Ritter
Keyword(s):  
Bisphenol A ◽  
Chronic Exposure ◽  
Follicle Culture ◽  
Low Concentration ◽  
Germinal Vesicles ◽  
Metaphase Ii Oocytes

Induction of DNA replication in germinal vesicles and in nuclei formed in maturing mouse oocytes by 6-DMAP treatment

Zygote ◽  
1997 ◽  
Vol 5 (3) ◽  
pp. 213-217 ◽  
Author(s):  
J. Fulka ◽  
N.L. First ◽  
C. Lee ◽  
J. Fulka ◽  
R.M. Moor
Keyword(s):  
Dna Replication ◽  
Dna Synthesis ◽  
Germinal Vesicle ◽  
The Other ◽  
Immature Oocytes ◽  
Mouse Oocytes ◽  
Germinal Vesicles ◽  
Immature Mouse ◽  
Condensed Dna ◽  
Metaphase Ii Oocytes

SummaryImmature mouse oocytes (germinal vesicle stage, GV), oocytes at different stages during maturation (prometaphase to anaphase I) and matured oocytes (metaphase II arrested) were cultured in 6-dimethylaminopurine (6-DMAP)-supplemented medium also containing bromodeoxyuridine for the assessment of DNA replication in these cells. Immature oocytes remained arrested at the GV stage and DNA replication was never detected in them. On the other hand, oocytes at the prometaphase to anaphase-telophase I stages responded to 6-DMAP treatment by forming nuclei which synthesised DNA. Mature (metaphase II) oocytes did not respond to 6-DMAP and their chromatin remained condensed. DNA synthesis could even be induced in GV-staged oocytes, but only when they were fused to freshly activated oocytes and incubated in 6-DMAP-supplemented medium.

Antibacterial effect of chronic exposure of low concentration ZnO nanoparticles onE. coli

2013 ◽  
Vol 48 (8) ◽  
pp. 871-878 ◽  
Author(s):  
R.K. Dutta ◽  
Bhavani P. Nenavathu ◽  
Mahesh K. Gangishetty ◽  
A.V.R. Reddy
Keyword(s):  
Zno Nanoparticles ◽  
Chronic Exposure ◽  
Antibacterial Effect ◽  
Low Concentration

scholarly journals CHRONIC EXPOSURE TO BISPHENOL A PRODUCES MORPHOLOGICAL DERANGEMENTS IN LIVER, KIDNEY AND HEART IN RATS

2018 ◽  
Vol 26 (1) ◽  
Author(s):  
Mahendra K. Pant ◽  
Jayanti Pant ◽  
Shripad B. Deshpande
Keyword(s):  
Bisphenol A ◽  
Chronic Exposure ◽  
Food Packaging ◽  
Structural Changes ◽  
Histopathological Examination ◽  
Oral Route ◽  
Female Rats ◽  
Histopathological Study ◽  
Portal Triad ◽  
Study Results

Introduction: Bisphenol A (BPA), an estrogenic compound is used in the manufacturing of plastics and also as coating for the inner linings of food packaging containers. It is reported to be an endocrine disruptor and produce toxicity in various organs. Since BPA exposure mainly occurs in the form of oral ingestion in humans, the present study was performed to examine the effects produced by the chronic exposure to BPA orally on kidney, liver and heart in rats. Material & Methods: Adult female rats of Charles Foster strain were used for the study. The rats were divided into two groups (n=6 in each group). In group 1, the rats were provided with food and water ad libitum whereas the rats of group 2 were fed with BPA containing pellets (2 µg/kg body weight/day) for 30 days. Thereafter the heart, kidneys and liver were excised and processed for histopathological study. Results: The histopathological examination of the organs in BPA fed rats showed major structural changes which manifested as loss of normal cytoarchitecture in all the three organs. Further there was significant reduction in the number of glomeruli in kidneys, degenerative changes in liver in the form of damage to portal triad and in heart the intercalated discs were damaged. Conclusion: Chronic exposure to BPA by oral route produces renal toxicity as loss of glomeruli; hepatotoxicity as cytoarchitecture loss and cardiotoxicity as damage of intercalated disc changes.

scholarly journals Chronic Exposure to High Doses of Bisphenol A Exhibits Significant Atrial Proarrhythmic Effects in Healthy Adult Rats

2021 ◽  
Vol 31 (3) ◽  
pp. 587-595
Author(s):  
Vasile Bogdan HALATIU ◽  
◽  
Alkora Ioana BALAN ◽  
Dan Alexandru COZAC ◽  
Remus BOBARNAC ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Chronic Exposure ◽  
Atrial Pacing ◽  
Autonomic Tone ◽  
Adult Rats ◽  
Female Wistar Rats ◽  
High Doses ◽  
Premature Beats

Objectives: We aimed to evaluate the effects of chronic exposure to bisphenol A (BPA) on atrial fibrillation (AF) occurrence in rats. Methods: Twenty-two healthy female Wistar rats were randomized into three groups: Control (no BPA; n=7), BPA (exposed to usual BPA doses; 50 μg/kg/day, 9 weeks; n=7), and hBPA (exposed to high BPA doses; 25 mg/kg/day, 9 weeks; n=8). 24-h ECG monitoring was performed using radiotelemetry ECG devices prior to and after transesophageal atrial pacing. Spontaneous and pacing-induced atrial arrhythmias, autonomic tone, and in vivo an in vitro atrial arrhythmogenicity-related parameters were evaluated. Results: All studied parameters were similar between Control and BPA (all p>0.05). However, compared to Control, hBPA presented more atrial premature beats both at baseline (p=0.04) and after pacing (p=0.03), more AF episodes (p<0.001) and of longer duration (p=0.02) following transesophageal stimulation, and significantly higher vagal tone (all p<0.05). Conclusions: Chronic exposure to high, but not usual BPA doses induced significant atrial proarrhythmic effects in healthy rats, and this may be at least partially due to BPA-induced vagal hyperactivation. Exposure to high BPA doses, such as that occurring in plastics industry workers, could favor AF occurrence even in the absence of underlying cardiovascular disease.

scholarly journals Multi-Systemic Alterations by Chronic Exposure to a Low Dose of Bisphenol A in Drinking Water: Effects on Inflammation and NAD+-Dependent Deacetylase Sirtuin1 in Lactating and Weaned Rats

2021 ◽  
Vol 22 (18) ◽  
pp. 9666
Author(s):  
Antonietta Santoro ◽  
Marika Scafuro ◽  
Jacopo Troisi ◽  
Giuseppe Piegari ◽  
Paola Di Pietro ◽  
...  
Keyword(s):  
Drinking Water ◽  
Adipose Tissue ◽  
Bisphenol A ◽  
Inflammatory Cytokines ◽  
Chronic Exposure ◽  
Adult Life ◽  
Sirtuin 1 ◽  
Cerebral Ventricles ◽  
Histone Deacetylase 1 ◽  
Significant Difference

Bisphenol A (BPA) is largely used as a monomer in some types of plastics. It accumulates in tissues and fluids and is able to bypass the placental barrier, affecting various organs and systems. Due to huge developmental processes, children, foetuses, and neonates could be more sensitive to BPA-induced toxicity. To investigate the multi-systemic effects of chronic exposure to a low BPA dose (100 μg/L), pregnant Wistar rats were exposed to BPA in drinking water during gestation and lactation. At weaning, newborn rats received the same treatments as dams until sex maturation. Free and conjugated BPA levels were measured in plasma and adipose tissue; the size of cerebral ventricles was analysed in the brain; morpho-functional and molecular analyses were carried out in the liver with a focus on the expression of inflammatory cytokines and Sirtuin 1 (Sirt1). Higher BPA levels were found in plasma and adipose tissue from BPA treated pups (17 PND) but not in weaned animals. Lateral cerebral ventricles were significantly enlarged in lactating and weaned BPA-exposed animals. In addition, apart from microvesicular steatosis, liver morphology did not exhibit any statistically significant difference for morphological signs of inflammation, hypertrophy, or macrovesicular steatosis, but the expression of inflammatory cytokines, Sirt1, its natural antisense long non-coding RNA (Sirt1-AS LncRNA) and histone deacetylase 1 (Hdac1) were affected in exposed animals. In conclusion, chronic exposure to a low BPA dose could increase the risk for disease in adult life as a consequence of higher BPA circulating levels and accumulation in adipose tissue during the neonatal period.

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