scholarly journals CHRONIC EXPOSURE TO BISPHENOL A PRODUCES MORPHOLOGICAL DERANGEMENTS IN LIVER, KIDNEY AND HEART IN RATS

2018 ◽  
Vol 26 (1) ◽  
Author(s):  
Mahendra K. Pant ◽  
Jayanti Pant ◽  
Shripad B. Deshpande
Keyword(s):  
Bisphenol A ◽  
Chronic Exposure ◽  
Food Packaging ◽  
Structural Changes ◽  
Histopathological Examination ◽  
Oral Route ◽  
Female Rats ◽  
Histopathological Study ◽  
Portal Triad ◽  
Study Results

Introduction: Bisphenol A (BPA), an estrogenic compound is used in the manufacturing of plastics and also as coating for the inner linings of food packaging containers. It is reported to be an endocrine disruptor and produce toxicity in various organs. Since BPA exposure mainly occurs in the form of oral ingestion in humans, the present study was performed to examine the effects produced by the chronic exposure to BPA orally on kidney, liver and heart in rats. Material & Methods: Adult female rats of Charles Foster strain were used for the study. The rats were divided into two groups (n=6 in each group). In group 1, the rats were provided with food and water ad libitum whereas the rats of group 2 were fed with BPA containing pellets (2 µg/kg body weight/day) for 30 days. Thereafter the heart, kidneys and liver were excised and processed for histopathological study. Results: The histopathological examination of the organs in BPA fed rats showed major structural changes which manifested as loss of normal cytoarchitecture in all the three organs. Further there was significant reduction in the number of glomeruli in kidneys, degenerative changes in liver in the form of damage to portal triad and in heart the intercalated discs were damaged. Conclusion: Chronic exposure to BPA by oral route produces renal toxicity as loss of glomeruli; hepatotoxicity as cytoarchitecture loss and cardiotoxicity as damage of intercalated disc changes.

Effects of bisphenol A treatment during pregnancy on kidney development in mice: a stereological and histopathological study

2017 ◽  
Vol 9 (2) ◽  
pp. 208-214 ◽  
Author(s):  
P. Nuñez ◽  
T. Fernandez ◽  
M. García-Arévalo ◽  
P. Alonso-Magdalena ◽  
A. Nadal ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Structural Changes ◽  
Kidney Development ◽  
Histopathological Examination ◽  
Female Offspring ◽  
Histopathological Study ◽  
Tissue Samples ◽  
Control Female ◽  
Increased Risk ◽  
Significant Difference

Bisphenol A (BPA) is a chemical found in plastics that resembles oestrogen in organisms. Developmental exposure to endocrine-disrupting chemicals, such as BPA, increases the susceptibility to type 2 diabetes (T2DM) and cardiovascular diseases. Animal studies have reported a nephron deficit in offspring exposed to maternal diabetes. The aim of this study was to investigate the prenatal BPA exposure effects on nephrogenesis in a mouse model that was predisposed to T2DM. This study quantitatively evaluated the renal structural changes using stereology and histomorphometry methods. The OF1 pregnant mice were treated with a vehicle or BPA (10 or 100 μg/kg/day) during days 9–16 of gestation (early nephrogenesis). The 30-day-old offspring were sacrificed, and tissue samples were collected and prepared for histopathological and stereology studies. Glomerular abnormalities and reduced glomerular formation were observed in the BPA offspring. The kidneys of the BPA10 and BPA100 female offspring had a significantly lower glomerular number and density than those of the CONTROL female offspring. The glomerular histomorphometry revealed a significant difference between the female and male CONTROL offspring for the analysed glomerular parameters that disappeared in the BPA10 and BPA100 offspring. In addition, the kidney histopathological examination showed typical male cuboidal epithelial cells of the Bowman capsule in the female BPA offspring. Exposure to environmentally relevant doses of BPA during embryonic development altered nephrogenesis. These structural changes could be associated with an increased risk of developing cardiometabolic diseases later in life.

Investigation of the effects of bisphenol-A exposure on lymphoid system in prenatal stage

2020 ◽  
Vol 36 (7) ◽  
pp. 502-513
Author(s):  
Işil Aydemir ◽  
Caner Özbey ◽  
Oktay Özkan ◽  
Şadiye Kum ◽  
Mehmet İbrahim Tuğlu
Keyword(s):  
Lymph Node ◽  
Bisphenol A ◽  
Tissue Damage ◽  
Corn Oil ◽  
Histopathological Examination ◽  
Female Rats ◽  
Male Rats ◽  
High Dose ◽  
Organ Function ◽  
Pregnant Rats

Bisphenol-A (BPA) used in the production of plastic materials is a temperature-soluble agent. It also has a steroid hormone-like activity; therefore, it poses a danger to human health. In our study, we aimed to investigate the effects of BPA on lymph node and spleen in male rats exposed to this agent during prenatal stage. The pregnant female rats were divided into four groups: control, sham, low dose (300 µg/kg BPA), and high dose (900 µg/kg BPA). BPA was dissolved in 1 mL of corn oil and administered to the pregnant rats every day during pregnancy. On the 21st and 45th day after the birth, male rats’ lymph node and spleen samples were taken and histopathological examination was performed. Samples were stained with hematoxylin and eosin to determine the general histological appearance, and with CD3 and CD20 immunohistochemically. The results of staining were evaluated by H-score, and statistical analysis was performed. In the samples, BPA applications were not found to cause significant tissue damage. But there was a significant decrease in the immunoreactivities of CD3 and CD20 after BPA applications in both 21st and 45th day samples. After high dose BPA administration, decreased CD3 immunoreactivity was statistically significant. It is thought that BPA does not cause histologically significant tissue damage, but it may impair organ function at cellular level. The investigation of molecules involved in organ function will be useful in revealing the mechanisms that will cause dysfunction.

scholarly journals Protective effect of melatonin on the rat lung following exposure to 900-MHz electromagnetic field: a stereological and histopathological study

2021 ◽  
Vol 38 (2) ◽  
pp. 55-60
Author(s):  
Ahmad Yahyazadeh ◽  
Elfide Gizem Kıvrak ◽  
Gülüna Erdem Koç
Keyword(s):  
Electromagnetic Field ◽  
Blood Vessels ◽  
Histopathological Examination ◽  
Marked Change ◽  
Female Rats ◽  
Control Group ◽  
Albino Rats ◽  
Histopathological Study ◽  
Rat Lung ◽  
The Mean

Long-term use of cell phone emitting electromagnetic field (EMF) has increased concerns regarding histopathological change in vital organs. Our present study was aimed to investigate the effect of 900 megahertz (MHz) EMF on the rat lungs, as well as the possible efficacy of melatonin (MEL) on lung tissues. Fifteen female Wistar albino rats were randomly selected and assigned into five groups as follow: control (CONT), SHAM, EMF, melatonin (MEL) and EMF+MEL group. Subsequently, female rats were then mated, and pregnant rats underwent the experimental application for 21 days. After parturition, 6 pups (2 pups from each mother) were randomly chosen and maintained for 4 weeks. At the end of the 4th week, the lung tissues of all groups were used for the stereological analysis and histopathological examination. We found that the mean volumes of the alveoli, bronchioles and blood vessels were significantly lower in the EMF group compared to the control group (p<0.05). In the EMF+MEL group, the mean volumes of the alveoli, bronchioles and blood vessels were significantly increased compared to the EMF group. Our histopathological results showed marked change in the lung tissues. We speculated that exposure to EMF caused the damage to the rat lung tissues, and that MEL administration alleviated 900 MHz EMF-induced complications.

scholarly journals Bisphenol a Hormonal Disrupture and Preventive Effect of Rose Water and Clove Oil

2020 ◽  
Vol 11 (2) ◽  
pp. 8780-8803
Keyword(s):  
Bisphenol A ◽  
Corn Oil ◽  
Histopathological Examination ◽  
Female Rats ◽  
Male Rats ◽  
Histopathological Analysis ◽  
Clove Oil ◽  
Male And Female ◽  
Male And Female Rats ◽  
Stimulating Hormone

Bisphenol A (BPA) which considered synthetic estrogen that is an essential component of many plastic industries. This research was done to see the impacts of exposure of BPA on reproductive organs and hormonal levels in male and female albino Sprague-Dawley rats. The protective effect of rose water and clove oil on BPA was investigated. Ninety rats were divided into 18 groups, 9 groups of males and they are like for females. Rats were exposed to different oral gavage route 3 times a week by doses of BPA (20 µg, 20 mg, 200 mg) /kg b.wt for 6 weeks and BPA was solubilized in corn oil. BPA induced a significant decrease in total and free testosterone in male rats, in contrast to a significant increase in thyroid-stimulating hormone (TSH), progesterone, estrogen (E2), and prolactin (PRL), while a decrease in Follicle-stimulating hormone (FSH) compared to control groups. Histopathological examination revealed that rosewater and clove oil reduced testes and ovary damages induced by BPA. Rosewater and clove oil components were scanned using GC/MS, which showed that rosewater and clove oil contains phenols, flavonoids, and these inevitably confirm that a prominent role in preventing the damage during treatment. Results indicated that the used doses of BPA disrupted the sex hormone levels in both male and female rats caused reproductive impaired. The chemical and histopathological analysis results indicated that clove oil and rose water improved the adverse effect of BPA. Rosewater and clove oil improved the changes which were stimulated by BPA.

scholarly journals Lycopene: Hepatoprotective and Antioxidant Effects toward Bisphenol A-Induced Toxicity in Female Wistar Rats

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Haidy G. Abdel-Rahman ◽  
Heba M. A. Abdelrazek ◽  
Dalia W. Zeidan ◽  
Rasha M. Mohamed ◽  
Aaser M. Abdelazim
Keyword(s):  
Bisphenol A ◽  
Wistar Rats ◽  
Histopathological Examination ◽  
Female Rats ◽  
Hepatic Tissue ◽  
Control Group ◽  
Cytotoxic Effects ◽  
Female Wistar Rats ◽  
Low Levels ◽  
Group 10

Bisphenol A (BPA)—an endocrine disruptor xenoestrogen—is widely spread in the environment. Lycopene (LYC) is an antioxidant phytochemical carotenoid. The hereby study was designed to verify the deleterious effect of BPA on cyclic female rats’ hepatic tissue as well as evaluation of the effect of LYC toward BPA hepatic perturbation. Twenty-eight female Wistar rats were allocated equally into four groups: control group, LYC group (10 mg/kg B.wt), BPA group (10 mg/kg B.wt), and BPA + LYC group (the same doses as former groups). The treatments were given daily via gavage to the rats for 30 days. The rats in BPA displayed high activities of serum liver enzymes with low levels of total proteins (TP) and albumin. Moreover, BPA induced hepatic oxidative stress via depletion of antioxidant enzymes concomitant with augmentation of lipid peroxidation, increased comet tail DNA %, and overexpression of caspase-3. Meanwhile, LYC administration reduced the cytotoxic effects of BPA on hepatic tissue, through improving the liver function biomarkers and oxidant-antioxidant state as well as DNA damage around the control values. These findings were confirmed by hepatic histopathological examination. Finally, LYC credited to have a noticeable protective effect versus BPA provoked oxidative injury and apoptosis of the liver tissue.

Evaluation of Breast Lumps by Ultrasound and Correlation with Histological Findings

2020 ◽  
Vol 23 (1) ◽  
pp. 10-12
Author(s):  
Nelema Jahan ◽  
Md. Mamunur Rahman ◽  
Mohammad Shahidul Alam ◽  
Md Saiful Islam
Keyword(s):  
Histopathological Examination ◽  
Breast Diseases ◽  
Histopathological Study ◽  
Common Symptom ◽  
Breast Lump ◽  
Histopathological Findings ◽  
Malignant Breast ◽  
Breast Lumps ◽  
One Year ◽  
A Prospective Study

Background: A breast lump is the most common symptom associated with both benign and malignant breast diseases. Therefore, a distinction of benign from malignant lump is of importance for proper management. Though a definitive diagnosis is possible with imaging for all the lesions, histopathological study is proven essential for confirming the diagnosis. Objective: The objective of this study was to evaluate the role of USG and histopathological findings of different breast lump in diagnosis and their comparison. Methods: A prospective study was conducted over a period of one year from January 2017 to December 2017. A total of 116 patients were included in this study. All breast lumps underwent surgery and the ultrasound findings of these lumps were compared with the histopathological findings. Data were collected from these patients by a preformed questionnaire and finally the data were analyzed. Results: Out of 116 patients only 21 cases were reported as malignant in ultrasound report but histopathology revealed 31 malignant patients. On histopathological examination 10 benign cases turned out to be malignant. Conclusion: The present study was undertaken to evaluate in diagnosing breast mass lesions individually by ultrasound and compared with histopathology for definitive management of a patient. Journal of Surgical Sciences (2019) Vol. 23 (1) : 10-12

The effect of dexamethasone on mucosal immunity of uterine tissue during pregnancy in rat

2019 ◽  
Vol 20 (1) ◽  
pp. 75-84
Keyword(s):  
Early Pregnancy ◽  
Histopathological Examination ◽  
Early Period ◽  
Interleukin 10 ◽  
Treated Group ◽  
Female Rats ◽  
Male Rats ◽  
Mrna Levels ◽  
Uterine Tissue ◽  
Leukocytic Infiltration

Disturbances in early pregnancy immunity affect embryo development, endometrial receptivity, placental development, fetal growth and lead to subfertility, dexamethasone is a synthetic glucocorticoid used for treatment of various complications. Immune cells and cytokines were examined during the early pregnancy in twenty-four female rats and six male rats for mating. Rats were grouped into two group control and dexamethasone treated by a dose of 50µgm/kgm body weight daily starting from one week before mating and persisted for one week after pregnancy. Blood samples were collected from each rat at 5hrs and at 1,3,7 day of pregnancy. Extracted RNA was subjected to real time PCR to determine mRNA levels for immune related genes interleukin1a(IL1A) and interleukin 10(IL10). Histopathological examination was done to uterus in order to detect leukocyte infiltration in uterine tissue. Results showed that significant increase in white blood cell count mainly eosinophil at 5hrs and lymphocyte at three and seven day of pregnancy of dexamethasone treated group. Moreover, TNF, C-reactive protein and progesterone were increased mainly at seven day of pregnancy of dexamethasone treated group. Similarly, interleukin 1alpha and interleukin 10 significantly increased at 5hrs and one day of pregnancy of dexamethasone treated group. In contrast, serum levels of total antioxidant capacity and estrogen were decreased significantly at 5hrs and seven day in dexamethasone treated group. Histopathological examination of uterus revealed leukocytic infiltration especially neutrophil and few eosinophils at five hours and one day of gestation then eosinophil become absent at 3day and seven day of dexamethasone group. Epithelial height and uterine gland diameter significantly increased at 5hrs, three day and seven days of gestation of dexamethasone treated group. The present investigation demonstrated that using of dexamethasone by dose of 50µgm/kgm during early pregnancy had a conflicting impact on some immune cytokines and parameters and may reflect a harmful response of immune system toward early period of pregnancy

A fluorescent aptasensor based on berberine for ultrasensitive detection of bisphenol A in tap water

2021 ◽  
Author(s):  
Yuxin Wei ◽  
Yangyang Zhou ◽  
Yanli Wei ◽  
Chuan Dong ◽  
Li Wang
Keyword(s):  
Bisphenol A ◽  
Human Health ◽  
Food Packaging ◽  
Tap Water ◽  
Water Systems ◽  
Ultrasensitive Detection ◽  
Its Analysis ◽  
Potential Impact ◽  
Fluorescent Aptasensor

The residues of bisphenol A (BPA) in food packaging and water systems have potential impact on human health, therefore, its analysis and detection has drawn people's attention. In this work,...

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