Characterization of Campylobacter species in Spanish retail from different fresh chicken products and their antimicrobial resistance

The poultry industry in Colombia has implemented several changes and measures in chicken processing to improve sanitary operations and control pathogens’ prevalence. However, there is no official in-plant microbial profile reference data currently available throughout the processing value chains. Hence, this research aimed to study the microbial profiles and the antimicrobial resistance of Salmonella isolates in three plants. In total, 300 samples were collected in seven processing sites. Prevalence of Salmonella spp. and levels of Enterobacteriaceae were assessed. Additionally, whole-genome sequencing was conducted to characterize the isolated strains genotypically. Overall, the prevalence of Salmonella spp. in each establishment was 77%, 58% and 80% for plant A, B, and C. The mean levels of Enterobacteriaceae in the chicken rinsates were 5.03, 5.74, and 6.41 log CFU/mL for plant A, B, and C. Significant reductions were identified in the counts of post-chilling rinsate samples; however, increased levels were found in chicken parts. There were six distinct Salmonella spp. clusters with the predominant sequence types ST32 and ST28. The serotypes Infantis (54%) and Paratyphi B (25%) were the most commonly identified within the processing plants with a high abundance of antimicrobial resistance genes.

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Emerging Options for the Diagnosis of Bacterial Infections and the Characterization of Antimicrobial Resistance

International Journal of Molecular Sciences ◽

10.3390/ijms22010456 ◽

2021 ◽

Vol 22 (1) ◽

pp. 456

Author(s):

Simone Rentschler ◽

Lars Kaiser ◽

Hans-Peter Deigner

Keyword(s):

Antimicrobial Resistance ◽

Bacterial Infections ◽

Point Of Care ◽

Rapid Identification ◽

Adequate Treatment ◽

Resistance Patterns ◽

Detection And Diagnosis ◽

Patient Prognosis ◽

Point Of Care Detection

Precise and rapid identification and characterization of pathogens and antimicrobial resistance patterns are critical for the adequate treatment of infections, which represent an increasing problem in intensive care medicine. The current situation remains far from satisfactory in terms of turnaround times and overall efficacy. Application of an ineffective antimicrobial agent or the unnecessary use of broad-spectrum antibiotics worsens the patient prognosis and further accelerates the generation of resistant mutants. Here, we provide an overview that includes an evaluation and comparison of existing tools used to diagnose bacterial infections, together with a consideration of the underlying molecular principles and technologies. Special emphasis is placed on emerging developments that may lead to significant improvements in point of care detection and diagnosis of multi-resistant pathogens, and new directions that may be used to guide antibiotic therapy.

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Risk characterization of antimicrobial resistance of Salmonella in meat products

Food Control ◽

10.1016/j.foodcont.2015.04.001 ◽

2015 ◽

Vol 57 ◽

pp. 18-23 ◽

Cited By ~ 11

Author(s):

Eva Doménech ◽

Ana Jiménez-Belenguer ◽

Rosa Pérez ◽

María Antonia Ferrús ◽

Isabel Escriche

Keyword(s):

Antimicrobial Resistance ◽

Meat Products ◽

Risk Characterization

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1667. Change in characteristics of community-onset ciprofloxacin-resistant E. coli isolates causing community-acquired acute pyelonephritis in South Korea

Open Forum Infectious Diseases ◽

10.1093/ofid/ofaa439.1845 ◽

2020 ◽

Vol 7 (Supplement_1) ◽

pp. S820-S820

Author(s):

Bongyoung Kim ◽

Ki Tae Kwon ◽

Seong-yeol Ryu ◽

Seong-Heon Wie ◽

Hyun-uk Jo ◽

...

Keyword(s):

Antimicrobial Resistance ◽

South Korea ◽

Molecular Characterization ◽

Acute Pyelonephritis ◽

Multilocus Sequence Typing ◽

Quinolone Resistance ◽

E Coli ◽

Extended Spectrum ◽

Community Onset

Abstract Background The aim of this study was to examine the change in characteristics of community-onset ciprofloxacin-resistant (CIP-R) E. coli isolates causing community-acquired acute pyelonephritis (CA-APN) in South Korea between 2010-2011 and 2017-2018. Methods E. coli samples isolated from the blood or urine were collected from patients with CA-APN aged 19 years and more who were admitted to 8 Korean hospitals from September 2017 to August 2018, prospectively. One isolate was collected from each patient. Phylogenetic typing, multilocus sequence typing (MLST), and molecular characterization of β-lactamase resistance and plasmid-mediated quinolone resistance (PMQR) determinants were performed. The data were compared with those from the previous study with same design in 2010-2011. Results A total of 346 and 300 isolates were collected during 2017-2018 and 2010-2011, respectively. Among them, 76 (22.0%) and 77 (25.7%) were CIP-R isolates. Significantly higher antimicrobial resistance against ampicillin (75.7% vs. 100%, P < 0.001) and cefotaxime (23.9% vs. 77.9%, P < 0.001) were observed for isolates in 2017-2018 compared to those in 2010-2011. The proportion of phylogenic group B2 had increased significantly (44.7% vs. 79.2%, P < 0.001). As for MLST, the proportion of ST131 (27.6% vs. 66.2%, P < 0.001) had increased while that of ST393 (18.4% vs. 3.9%, P =0.004) had decreased significantly. Higher proportion of CIP-R E. coli isolates in 2017-2018 had extended-spectrum β-lactamase (ESBL)/plasmid-mediated AmpC β-lactamase (PABL) (23.7% vs. 79.2%, P < 0.001) and PMQR determinant (11.8% vs. 40.8%, P < 0.001) compared to those in 2010-2011. Phlogenetic tree Analyzed by SplitsTree Conclusion Among uropathogenic CIP-R E. coli isolates in South Korea, ST131 predominance had become more prominent and the proportion of containing ESBL/PABL and/or PMQR determinants had increased. Disclosures All Authors: No reported disclosures

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Molecular characterization of the antimicrobial resistance of Riemerella anatipestifer isolated from ducks

Veterinary Microbiology ◽

10.1016/j.vetmic.2012.03.005 ◽

2012 ◽

Vol 158 (3-4) ◽

pp. 376-383 ◽

Cited By ~ 30

Author(s):

Na Sun ◽

Jian-Hua Liu ◽

Fan Yang ◽

Da-Chuan Lin ◽

Guang-Hui Li ◽

...

Keyword(s):

Antimicrobial Resistance ◽

Molecular Characterization ◽

Riemerella Anatipestifer

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Molecular characterization of Salmonella enterica serotype Enteritidis isolates from food and human samples by serotyping, antimicrobial resistance, plasmid profiling, (GTG)5-PCR and ERIC-PCR

New Microbes and New Infections ◽

10.1016/j.nmni.2016.07.016 ◽

2016 ◽

Vol 14 ◽

pp. 24-30 ◽

Cited By ~ 8

Author(s):

F. Fardsanei ◽

F. Nikkhahi ◽

B. Bakhshi ◽

T. Zahraei Salehi ◽

I. Asrafi Tamai ◽

...

Keyword(s):

Antimicrobial Resistance ◽

Molecular Characterization ◽

Salmonella Enterica ◽

Human Samples ◽

Resistance Plasmid ◽

Plasmid Profiling ◽

Salmonella Enterica Serotype Enteritidis

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Microbial profile, antimicrobial resistance, and molecular characterization of diabetic foot infections in a university hospital

GERMS ◽

10.18683/germs.2021.1239 ◽

2021 ◽

Vol 11 (1) ◽

pp. 39-51

Author(s):

Azza A Ismail ◽

Marwa A Meheissen ◽

Talaat A Abd Elaaty ◽

Nermine E Abd-Allatif ◽

Heba S Kassab

Keyword(s):

Antimicrobial Resistance ◽

Molecular Characterization ◽

Diabetic Foot ◽

University Hospital ◽

Microbial Profile ◽

Diabetic Foot Infections

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Characterization of cefotaxime resistant Escherichia coli isolated from broiler farms in Ecuador

10.1101/462994 ◽

2018 ◽

Author(s):

Christian Vinueza-Burgos ◽

David Ortega-Paredes ◽

Cristian Narváez ◽

Lieven De Zutter ◽

Jeannete Zurita

Keyword(s):

Antimicrobial Resistance ◽

Latin American ◽

Production Systems ◽

Diffusion Method ◽

Poultry Production ◽

Disk Diffusion Method ◽

E Coli ◽

Resistance Patterns ◽

Latin American Countries

AbstractAntimicrobial resistance (AR) is a worldwide concern. Up to a 160% increase in antibiotic usage in food animals is expected in Latin American countries. The poultry industry is an increasingly important segment of food production and contributor to AR. The objective of this study was to evaluate the prevalence, AR patterns and the characterization of relevant resistance genes in Extended Spectrum β-lactamases (ESBL) and AmpC E. coli from large poultry farms in Ecuador. Sampling was performed from June 2013 to July 2014 in 6 slaughterhouses that slaughter broilers from 115 farms totaling 384 flocks. Each sample of collected caeca was streaked onto TBX agar supplemented with cefotaxime (3 mg/l). In total, 176 isolates were analyzed for antimicrobial resistance patterns by the disk diffusion method and for blaCTX-M, blaTEM, blaCMY, blaSHV, blaKPC, and mcr-1 by PCR and sequencing. ESBL and AmpC E. coli were found in 362 flocks (94.3%) from 112 farms (97.4%). We found that 98.3% of the isolates were multi-resistant to antibiotics. Low resistance was observed for ertapenem and nitrofurantoin. The most prevalent ESBL genes were the blaCTX-M (90.9%) blaCTX-M-65, blaCTX-M-55 and blaCTX-M-3 alleles. Most of the AmpC strains presented the blaCMY-2 gene. Three isolates showed the mcr-1 gene. Poultry production systems represent a hotspot for antimicrobial resistance in Ecuador, possibly mediated by the extensive use of antibiotics. Monitoring this sector in national and regional plans of antimicrobial resistance surveillance should therefore be considered.

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