Development of novel high-selective extraction approach of carotenoproteins from blue crab (Portunus segnis) shells, contribution to the qualitative analysis of bioactive compounds by HR-ESI-MS

2020 ◽  
Vol 302 ◽  
pp. 125334 ◽  
Author(s):  
Marwa Hamdi ◽  
Rim Nasri ◽  
Nawel Dridi ◽  
Suming Li ◽  
Moncef Nasri
Keyword(s):  
Qualitative Analysis ◽  
Blue Crab ◽  
Bioactive Compounds ◽  
Selective Extraction ◽  
Esi Ms ◽  
Portunus Segnis

Development and Validation of RP-HPLC Method for Vicenin-2, Orientin, Cynaroside, Betulinic Acid, Genistein, and Major 8 Bioactive Constituents with LC-ESI-MS/MS profiling in Ocimum genus

2021 ◽  
Author(s):  
Aboli Girme ◽  
Prajkta Bhoj ◽  
Ganesh Saste ◽  
Sandeep Pawar ◽  
Amit Mirgal ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
Betulinic Acid ◽  
Gradient Elution ◽  
Carnosic Acid ◽  
Bioactive Constituents ◽  
Validation Data ◽  
Method Performance ◽  
Esi Ms ◽  
Stress Relieving ◽  
Quality Control Tool

Abstract Background Ocimum genus known as Tulsi or Basil is a prominent botanical class in Asian culture, especially in India. The leaves have an immunomodulatory, antioxidant, stress-relieving, and adaptogenic role in traditional and modern medicine, with prominent usage in herbal teas and nutraceuticals. Objective The HPLC-PDA method was developed and validated for vicenin-2, orientin, cynaroside, betulinic acid, genistein, with syringic acid, rosmarinic acid, eugenol, carnosic acid, oleanolic acid, ursolic acid, luteolin, apigenin for quantification and confirmed using a novel ESI -MS/MS method in the Ocimum samples. Method The methodology parameters were developed on the RP-C18 column with a gradient elution of 1 mL/min flow rate for 0.1% o-phosphoric acid and acetonitrile at 210 and 340 nm wavelengths. Result The validation data for 13 bioactive compounds showed good linearity (r2> 0.99) with sensitive LOD (0.034-0.684 µg/mL) and LOQ (0.100-2.068 µg/mL) with recoveries (83.66-101.53%).The results were found precise (RSD,<5.0%) and accurate (RE, -0.60-1.06) for the quantification. The method performance was verified and found robust by analyzing ten samples of O. tenuiflorum from the ten geographical states of India (RSD, < 5.0%). Conclusion The validated HPLC-PDA method was found selective and suitable for analyzing thirteen compounds in O. tenuiflorum and twelve cultivars from the Ocimum genus as a quality control tool. This method can be used in routine analysis as an inexpensive alternative to advanced techniques. Highlights This work is the first to report for vicenin-2, orientin, cynaroside, betulinic acid, and genistein, with simultaneous analysis of eight bioactive compounds in the Ocimum genus.

scholarly journals Bioactive Compounds in Sarcocornia and Arthrocnemum, Two Wild Halophilic Genera from the Iberian Peninsula

Plants ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 2218
Author(s):  
Irene Sánchez-Gavilán ◽  
Esteban Ramírez Chueca ◽  
Vicenta de la Fuente García
Keyword(s):  
Fatty Acids ◽  
Salt Tolerance ◽  
Ferulic Acid ◽  
Iberian Peninsula ◽  
Bioactive Compounds ◽  
Phenolic Acids ◽  
Coastal Marshes ◽  
Esi Ms ◽  
Tinto River ◽  
High Diversity

(1) Background: this study describes bioactive compounds in the following halophytes: Sarcocornia (S. alpini, S. pruinosa, and S. perennis) and Arthrocnemum (A. macrostachyum). The material comes from: coastal marshes in Tinto River, Guadiana River, and some interior provinces from the Iberian Peninsula. (2) Methods: the techniques used were Folin–Ciocalteu, GC-MS, and ESI-MS/MS. (3) Results: Five phenolic acids were found in Sarcocornia: trans-cinnamic, salicylic, veratric, coumaric, and caffeic acids. In addition, in Arthronemum, ferulic acid was also detected. The obtained flavonoids were cyanidin-3-O-arabinoside, luteolin-7-glucoside, dihydroquercetin, and p-coumaroyl-glucoside. They also presented fatty acids, such as palmitic, linoleic, and oleic acids in Sarcocornia, while palmitic, linolenic, and stearic acids were the main fatty acids in A. macrostachyum. (4) Conclusions: the high diversity of the compounds identified confirms the relation between nutritional interest and salt tolerance in halophytes.

scholarly journals Trends in valorization of the invasive crab Portunus segnis for cleaner production of chitin, chitosan, and protein hydrolysate

2021 ◽  
Author(s):  
Fadoua Jabeur ◽  
Sondes Mechri ◽  
Fethi Mensi ◽  
Ines Gharbi ◽  
Yosri Ben Naser ◽  
...  
Keyword(s):  
Blue Crab ◽  
Protein Hydrolysate ◽  
Radical Scavenging ◽  
Angiotensin I Converting Enzyme ◽  
Chitin And Chitosan ◽  
Recalcitrant Waste ◽  
Product Powder ◽  
Anoxybacillus Kamchatkensis ◽  
Portunus Segnis ◽  
Central Composite

Abstract The diversity of marine biomasses is a set of exploitable and renewable resources with application in several sectors. In this context, a co-culture based on three protease-producing bacterial isolates namely; Aeribacillus pallidus VP3, Lysinibacillus fusiformis C250R , and Anoxybacillus kamchatkensis M1V strains, was carried out in a medium based on the invasive blue crab Portunus segnis bio-waste. Optimization of proteases production was performed using a central composite design (CCD). The highest level of proteases production obtained was 8,809 U/mL in a medium comprising 75 g/L of Portunus segnis by-product powder (Pspp ). The recovered protein hydrolysate (P Hyd ) was found to be active towards radical scavenging power, and against angiotensin I-converting enzyme (ACE). The extraction efficiency of the blue crab chitin (BC) was achieved with a yield of 32%. Afterward, chitosan was prepared through chitin N -deacetylation with a yield of 52%, leading to an acetylation degree (AD) of 19% and solubility of 90%. The spectrum of chitin and chitosan were depicted by Fourier-transform infrared spectroscopy (FTIR). The biological value of Pspp and its obtained derivates were evidenced via accredited protocols. These data constitute a roadmap towards a circular and sustainable bio-economic strategy for a clean transformation of a recalcitrant waste to bio-based products.

scholarly journals Array of Metabolites in Italian Hericium erinaceus Mycelium, Primordium, and Sporophore

Molecules ◽  
2019 ◽  
Vol 24 (19) ◽  
pp. 3511 ◽  
Author(s):  
Federica Corana ◽  
Valentina Cesaroni ◽  
Barbara Mannucci ◽  
Rebecca Michela Baiguera ◽  
Anna Maria Picco ◽  
...  
Keyword(s):  
Dietary Supplements ◽  
Bioactive Compounds ◽  
Medicinal Mushroom ◽  
Wild Type ◽  
Hericium Erinaceus ◽  
Esi Ms ◽  
Therapeutic Properties ◽  
Erinacine A ◽  
First Time

Hericium erinaceus is a medicinal mushroom that contains many molecules promising a plethora of therapeutic properties. In this study, the strain H.e.2 (MicUNIPV, University of Pavia, Italy) was isolated from a sporophore collected in Tuscany (Italy). Mycelium, primordium, and wild type and cultivated sporophores were analyzed by HPLC-UV-ESI/MS. Erinacine A in the mycelium and hericenones C and D in the sporophores were quantified by comparison with their standard molecules. For the first time, H. erinaceus primordium was also investigated for the presence of these molecules. Comparing with the literature data, hericenes, molecules structurally similar to hericenones, were present in all our samples. The highest contents of hericenones C and D were detected in cultivated sporophores, compared to the wild type. The comparison of these data with those of another Italian H. erinaceus strain (H.e.1 MicUNIPV) was discussed. The results led us to select H. erinaceus strains more suitable for mycelium production or sporophore cultivation to obtain extracts with a higher content of bioactive compounds. This work provides a further step towards standardizing the procedures in the development of dietary supplements made from mushrooms.

scholarly journals Rapid Mass Spectrometric Study of a Supercritical CO2-extract from Woody Liana Schisandra chinensis by HPLC-SPD-ESI-MS/MS

Molecules ◽  
2020 ◽  
Vol 25 (11) ◽  
pp. 2689
Author(s):  
Mayya Razgonova ◽  
Alexander Zakharenko ◽  
Konstantin Pikula ◽  
Ekaterina Kim ◽  
Valery Chernyshev ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Mass Spectrometric Study ◽  
Selective Extraction ◽  
Extraction Methods ◽  
Schisandra Chinensis ◽  
Esi Ms ◽  
Naturally Occurring ◽  
Target Analytes ◽  
Carbon Dioxide Co2 ◽  
Rapid Mass

Woody liana Schisandra chinensis contains valuable lignans, which are phenylpropanoids with valuable biological activity. Among green and selective extraction methods, supercritical carbon dioxide (SC-CO2) was shown to be the method of choice for the recovery of these naturally occurring compounds. Carbon dioxide (CO2) was the solvent with the flow rate (10−25 g/min) with 2% ethanol as co-solvent. In this piece of work operative parameters and working conditions were optimized by experimenting with different pressures (200–400 bars) and temperatures (40–60 °C). The extraction time varied from 60 to 120 min. HPLC-SPD-ESI -MS/MS techniques were applied to detect target analytes. Twenty-six different lignans were identified in the S. chinensis SC-CO2 extracts.

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