Interaction of DPPC liposomes with cholesterol and food protein during in vitro digestion using Dynamic Light Scattering and FTIR spectroscopy analysis

Dynamic light scattering is a method that depends on the interaction of light with particles. This method can be used for measurements of narrow particle size distributions especially in the range of 2–500 nm. Sample polydispersity can distort the results, and we could not see the real populations of particles because big particles presented in the sample can screen smaller ones. Although the theory and mathematical basics of DLS technique are already well known, little has been done to determine its limits experimentally. The size and size distribution of artificially prepared polydisperse silver nanoparticles (NPs) colloids were studied using dynamic light scattering (DLS) and ultraviolet-visible (UV-Vis) spectroscopy. Polydisperse colloids were prepared based on the mixture of chemically synthesized monodisperse colloids well characterized by atomic force microscopy (AFM), transmission electron microscopy (TEM), DLS, and UV-Vis spectroscopy. Analysis of the DLS results obtained for polydisperse colloids reveals that several percent of the volume content of bigger NPs could screen completely the presence of smaller ones. The presented results could be extremely important from nanoparticles metrology point of view and should help to understand experimental data especially for the one who works with DLS and/or UV-Vis only.

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Multi-Step Concanavalin A Phase Separation and Early-Stage Nucleation Monitored Via Dynamic and Depolarized Light Scattering

Crystals ◽

10.3390/cryst9120620 ◽

2019 ◽

Vol 9 (12) ◽

pp. 620 ◽

Cited By ~ 3

Author(s):

Hévila Brognaro ◽

Sven Falke ◽

Celestin Nzanzu Mudogo ◽

Christian Betzel

Keyword(s):

Phase Separation ◽

Light Scattering ◽

Dynamic Light Scattering ◽

Protein Interactions ◽

Concanavalin A ◽

Early Stage ◽

Protein Crystallization ◽

Cluster Formation ◽

Macromolecular Network

Protein phase separation and protein liquid cluster formation have been observed and analysed in protein crystallization experiments and, in recent years, have been reported more frequently, especially in studies related to membraneless organelles and protein cluster formation in cells. A detailed understanding about the phase separation process preceding liquid dense cluster formation will elucidate what has, so far, been poorly understood—despite intracellular crowding and phase separation being very common processes—and will also provide more insights into the early events of in vitro protein crystallization. In this context, the phase separation and crystallization kinetics of concanavalin A were analysed in detail, which applies simultaneous dynamic light scattering and depolarized dynamic light scattering to obtain insights into metastable intermediate states between the soluble phase and the crystalline form. A multi-step mechanism was identified for ConA phase separation, according to the resultant ACF decay, acquired after an increase in the concentration of the crowding agent until a metastable ConA gel intermediate between the soluble and final crystalline phases was observed. The obtained results also revealed that ConA is trapped in a macromolecular network due to short-range intermolecular protein interactions and is unable to transform back into a non-ergodic solution.

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Temperature-Dependent Solubilization of the Hydrophobic Antiepileptic Drug Lamotrigine in Different Pluronic Micelles—A Spectroscopic, Heat Transfer Method, Small-Angle Neutron Scattering, Dynamic Light Scattering, and in Vitro Release Study

ACS Omega ◽

10.1021/acsomega.9b00939 ◽

2019 ◽

Vol 4 (6) ◽

pp. 11251-11262 ◽

Cited By ~ 9

Author(s):

Pankaj Singla ◽

Onkar Singh ◽

Shagun Sharma ◽

Kai Betlem ◽

Vinod K. Aswal ◽

...

Keyword(s):

Heat Transfer ◽

Light Scattering ◽

Dynamic Light Scattering ◽

Small Angle Neutron Scattering ◽

In Vitro Release ◽

Temperature Dependent ◽

Release Study ◽

Transfer Method ◽

Vitro Release

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Applicability and Limitations in the Characterization of Poly-Dispersed Engineered Nanomaterials in Cell Media by Dynamic Light Scattering (DLS)

Materials ◽

10.3390/ma12233833 ◽

2019 ◽

Vol 12 (23) ◽

pp. 3833 ◽

Cited By ~ 2

Author(s):

Arianna Marucco ◽

Elisabetta Aldieri ◽

Riccardo Leinardi ◽

Enrico Bergamaschi ◽

Chiara Riganti ◽

...

Keyword(s):

Light Scattering ◽

Dynamic Light Scattering ◽

Culture Media ◽

Raw 264.7 ◽

Pre Treatment ◽

The Stability ◽

Definition Of ◽

Cellular Tests

The dispersion protocol used to administer nanomaterials (NMs) in in vitro cellular tests might affect their toxicity. For this reason, several dispersion procedures have been proposed to harmonize the toxicological methods, allowing for the comparison of the data that were obtained by different laboratories. At the same time, several techniques and methods are available to monitor the identity of the NMs in the cell media. However, while the characterization of suspensions of engineered NMs having narrow size distribution may be easily performed, the description of aggregated NMs forming polydispersions is still challenging. In the present study, sub-micrometric/nanometric TiO2, SiO2, and CeO2 were dispersed in cell media by using two different dispersion protocols, with and without albumin (0.5%) and with different sonication procedures. Dynamic Light Scattering (DLS) was used to characterize NMs in stock solutions and culture media. Pitfalls that affect DLS measurements were identified and, guidance on a critical analysis of the results provided. The NMs were then tested for their cytotoxicity (LDH leakage) toward murine macrophages (RAW 264.7) and PMA-activated human monocytes (THP-1). As markers of pro-inflammatory response, nitric oxide (NO) and cytokine IL-1β production were measured on RAW 264.7 and THP-1 cells, respectively. The pre-treatment with albumin added to a strong sonication treatment increases the stability and homogeneity of the suspensions of nanometric samples, but not of the submicrometric-samples. Nevertheless, while TiO2 and CeO2 were non-cytotoxic in any conditions, differences in cytotoxicity, NO, and IL-1β releases were found for the SiO2, depending upon the protocol. Overall, the results suggest that there is no one-fits-all method valid for all NMs, since each class of NMs respond differently. The definition of validated procedures and parameters for the selection of the most appropriate method of dispersion for each class of NM appears to be a more efficacious strategy for the harmonization of the dispersion protocols.

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Characterization of Nanomaterial Dispersion in Solution Prior to In Vitro Exposure Using Dynamic Light Scattering Technique

Toxicological Sciences ◽

10.1093/toxsci/kfm240 ◽

2007 ◽

Vol 101 (2) ◽

pp. 239-253 ◽

Cited By ~ 661

Author(s):

Richard C. Murdock ◽

Laura Braydich-Stolle ◽

Amanda M. Schrand ◽

John J. Schlager ◽

Saber M. Hussain

Keyword(s):

Light Scattering ◽

Dynamic Light Scattering ◽

In Vitro Exposure ◽

Scattering Technique ◽

Dynamic Light Scattering Technique ◽

Light Scattering Technique

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Reliable size determination of nanoparticles using dynamic light scattering method for in vitro toxicology assessment

Toxicology in Vitro ◽

10.1016/j.tiv.2009.04.006 ◽

2009 ◽

Vol 23 (5) ◽

pp. 927-934 ◽

Cited By ~ 82

Author(s):

Haruhisa Kato ◽

Mie Suzuki ◽

Katsuhide Fujita ◽

Masanori Horie ◽

Shigehisa Endoh ◽

...

Keyword(s):

Light Scattering ◽

Dynamic Light Scattering ◽

Scattering Method ◽

Size Determination ◽

In Vitro Toxicology ◽

Dynamic Light Scattering Method ◽

Toxicology Assessment

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MACROMOLECULAR CROWDING IN BIOLOGICAL SYSTEMS: DYNAMIC LIGHT SCATTERING (DLS) TO QUANTIFY THE EXCLUDED VOLUME EFFECT (EVE)

Biophysical Reviews and Letters ◽

10.1142/s1793048006000215 ◽

2006 ◽

Vol 01 (03) ◽

pp. 317-325 ◽

Cited By ~ 21

Author(s):

KARTHIK S. HARVE ◽

MICHAEL RAGHUNATH ◽

RICKY R. LAREU ◽

RAJ RAJAGOPALAN

Keyword(s):

Light Scattering ◽

Dynamic Light Scattering ◽

Biological Systems ◽

Volume Effect ◽

Threshold Concentration ◽

Excluded Volume ◽

Matrix Deposition ◽

Excluded Volume Effect

Macromolecules crowd defined spaces, thereby excluding other like-sized molecules from the volume they occupy. These excluded-volume effect(s) (EVE) are well characterized for intracellular and partially for extracellular compartments such as blood plasma. We showed that EVE in fibroblast culture leads to faster enzymatic procollagen conversion and matrix deposition. Apparently, EVE can be applied to emulate in vivo conditions in an in vitro setting. Thus, we attempted to quantitatively capture the crowding potential of various macromolecules using dynamic light scattering under physiological conditions. We found that charged macromolecules like dextran sulfate (negative, 500 kDa) have a hydrodynamic radii of 46.4 ± 0.3 nm i.e. ~4 fold larger than that of neutral macromolecules like Ficoll (neutral, 400 kDa) and thus show greater EVE potential. At biologically effective concentrations viscosity was not increased. Unexpectedly, we observed a dramatic drop of hydrodynamic radii of all macromolecules tested above a threshold concentration. This suggested a hyper-crowding state in which the crowders compacted themselves mutually. We will use this hyper-crowding threshold to determine retrogradely rules that allow to predict the conditions for optimum crowding effects (such as the half-hyper-crowding concentration) in biological systems. We propose Dynamic Light Scattering (DLS) as a potential tool to estimate EVE in biotechnical applications.

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Molecular structure, morphology and growth mechanisms and rates of 5-hydroxymethyl furfural (HMF) derived humins

Green Chemistry ◽

10.1039/c5gc01938a ◽

2016 ◽

Vol 18 (7) ◽

pp. 1983-1993 ◽

Cited By ~ 156

Author(s):

George Tsilomelekis ◽

Michael J. Orella ◽

Zhexi Lin ◽

Ziwei Cheng ◽

Weiqing Zheng ◽

...

Keyword(s):

Molecular Structure ◽

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Light Scattering ◽

Dynamic Light Scattering ◽

Ftir Spectroscopy ◽

Growth Mechanisms ◽

Hydroxymethyl Furfural ◽

Structure Morphology ◽

Scanning Electron

Coupling ATR-FTIR spectroscopy, Scanning Electron Microscopy and Dynamic Light Scattering to understand the formation of 5-hydroxymethylfurfural (HMF) derived humins.

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