Bioactive properties of peptides obtained by enzymatic hydrolysis from protein byproducts of Porphyra columbina

Mussel production generates losses and waste since their commercialisation must be aligned with target market criteria. Since mussels are rich in proteins, their meat can be explored as a source of bioactive hydrolysates. Thus, the main objective of this study was to establish the optimal production conditions through two Box–Behnken designs to produce, by enzymatic hydrolysis (using subtilisin and corolase), hydrolysates rich in proteins and with bioactive properties. The factorial design allowed for the evaluation of the effects of three factors (hydrolysis temperature, enzyme ratio, and hydrolysis time) on protein/peptides release as well as antioxidant and anti-hypertensive properties of the hydrolysates. The hydrolysates produced using the optimised conditions using the subtilisin protease showed 45.0 ± 0.38% of protein, antioxidant activity via ORAC method of 485.63 ± 60.65 µmol TE/g of hydrolysate, and an IC50 for the inhibition of ACE of 1.0 ± 0.56 mg of protein/mL. The hydrolysates produced using corolase showed 46.35 ± 1.12% of protein, antioxidant activity of 389.48 ± 0.21 µmol TE/g of hydrolysate, and an IC50 for the inhibition of ACE of 3.7 ± 0.33 mg of protein/mL. Mussel meat losses and waste can be used as a source of hydrolysates rich in peptides with relevant bioactive properties, and showing potential for use as ingredients in different industries, such as food and cosmetics, contributing to a circular economy and reducing world waste.

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Effect of enzymatic hydrolysis on bioactive properties and allergenicity of cricket (Gryllodes sigillatus) protein

Food Chemistry ◽

10.1016/j.foodchem.2018.04.058 ◽

2018 ◽

Vol 262 ◽

pp. 39-47 ◽

Cited By ~ 37

Author(s):

Felicia Hall ◽

Philip E. Johnson ◽

Andrea Liceaga

Keyword(s):

Enzymatic Hydrolysis ◽

Bioactive Properties ◽

Gryllodes Sigillatus

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Enzymatic Hydrolysis of Blue Whiting (Micromesistius poutassou); Functional and Bioactive Properties

Journal of Food Science ◽

10.1111/j.1750-3841.2010.01877.x ◽

2010 ◽

Vol 76 (1) ◽

pp. C14-C20 ◽

Cited By ~ 44

Author(s):

Margret Geirsdottir ◽

Sjofn Sigurgisladottir ◽

Patricia Y. Hamaguchi ◽

Gudjon Thorkelsson ◽

Ragnar Johannsson ◽

...

Keyword(s):

Enzymatic Hydrolysis ◽

Blue Whiting ◽

Bioactive Properties ◽

Hydrolysis Of

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Flavour Improvement of Protein Hydrolysates Derived from Cephalopods Byproducts Using Maillard Reaction: A Short Review

ASM Science Journal ◽

10.32802/asmscj.2021.694 ◽

2021 ◽

Vol 15 ◽

pp. 1-13

Author(s):

Rabeta Mohd Salleh ◽

Ayu Shazwani Zulkipli

Keyword(s):

Enzymatic Hydrolysis ◽

Maillard Reaction ◽

Food Industry ◽

Value Added ◽

Short Review ◽

Protein Hydrolysates ◽

Food Ingredients ◽

Effective Utilization ◽

Bioactive Properties ◽

Plastein Reaction

The conversion of low-value byproducts to high value-added protein hydrolysates by enzymatic hydrolysis is among the top trends in the food industry. Protein hydrolysates provide an opportunity for effective utilization and are considered promising functional food ingredients. However, hydrolyzing effect contributes to taste and aroma defects such as fishy off-flavour and bitterness that impeded their application. Several procedures such as encapsulation, enzymatic hydrolysis with exopeptidase and plastein reaction and Maillard reaction have been compared to mask the undesirable flavour of protein hydrolysates. Maillard reaction has more potential to overcome these challenges and enhance the organoleptic properties of protein hydrolysates. All literature were accessed through available electronic databases. The revised overview can be helpful to explore the rational use of Maillard Reaction with reduced adverse effects. The reaction of Maillard-induced modification on bioactive properties of protein-derived peptides is well-positioned the beneficial effect and facilitate design to obtain applicable functional ingredients for food formulation.

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Metabolic Studies of Vitamin K1-14C and Menadione-14C in the Normal and Hepatectomized Rats

Thrombosis and Haemostasis ◽

10.1055/s-0038-1651216 ◽

1968 ◽

Vol 19 (03/04) ◽

pp. 383-388 ◽

Cited By ~ 6

Author(s):

R Losito ◽

C. A Owen ◽

E. V Flock ◽

Keyword(s):

Enzymatic Hydrolysis ◽

Oral Anticoagulants ◽

Urinary Metabolites ◽

The Other ◽

Vitamin K1 ◽

Vitamin K3 ◽

Metabolic Studies ◽

Intact Rats

SummaryThe metabolism of vitamin K1- 14C and menadione-14C (vitamin K3-14C) was studied in normal and hepateetomized rats. After the administration of menadione, about 70% of the 14C was excreted in the urine in 24 hrs in both types of rats. Two urinary metabolites were identified by enzymatic hydrolysis: one a glucuronide and the other a sulfate of reduced menadione. Thus, the liver is not necessary for the metabolism of menadione. In the vitamin K1 studies, the intact rats excreted only 10% of the 14C and the hepatectomized rats excreted less than 0.5%. The retention of vitamin K1 may explain its superiority over menadione as an antidote for overdosages of oral anticoagulants.

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From biorefineries to bioproducts: conversion of pretreated pulp from biorefining streams to lignocellulose nanofibers

TAPPI Journal ◽

10.32964/tj18.4.233 ◽

2019 ◽

Vol 18 (4) ◽

pp. 233-241

Author(s):

CHENGGUI SUN ◽

RICHARD CHANDRA ◽

YAMAN BOLUK

Keyword(s):

Energy Consumption ◽

Enzymatic Hydrolysis ◽

Rheological Behavior ◽

Material Basis ◽

Softwood Pulp ◽

Energy Consumptions ◽

Hardwood Pulp ◽

The Stability ◽

Lignocellulose Nanofibers

This study investigates the use of pretreatment and enzymatic hydrolysis side streams and conversion to lignocellulose nanofibers. We used a steam-exploded and partial enzymatic hydrolyzed hardwood pulp and an organosolv pretreated softwood pulp to prepare lignocellulose nanofibers (LCNF) via microfluidization. The energies applied on fibrillation were estimated to examine the energy consumption levels of LCNF production. The energy consumptions of the fibrillation processes of the hardwood LCNF production and the softwood LCNF production were about 7040-14080 kWh/ton and 4640 kWh/ton on a dry material basis, respectively. The morphology and dimension of developed hardwood and softwood LCNFs and the stability and rheological behavior of their suspensions were investigated and are discussed.

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