Protective effect of mulberry fruit anthocyanin on human hepatocyte cells (LO2) and Caenorhabditis elegans under hyperglycemic conditions

2017 ◽  
Vol 102 ◽  
pp. 213-224 ◽  
Author(s):  
Fujie Yan ◽  
Xin'an Chen ◽  
Xiaodong Zheng
Keyword(s):  
Caenorhabditis Elegans ◽  
Protective Effect ◽  
Human Hepatocyte ◽  
Mulberry Fruit ◽  
Hepatocyte Cells

Protective effect of Annona crassiflora on oxidative stress and Alzheimer’s models in Caenorhabditis elegans

2018 ◽  
Vol 128 ◽  
pp. S125
Author(s):  
Edson Lucas dos Santos ◽  
Natasha Leite ◽  
Laura Costa Alves de Araújo ◽  
José Tarcisio Giffoni de Carvalho ◽  
Kely de Picoli Souza
Keyword(s):  
Oxidative Stress ◽  
Caenorhabditis Elegans ◽  
Protective Effect ◽  
Annona Crassiflora

scholarly journals Astragalus Polysaccharide Suppresses 6-Hydroxydopamine-Induced Neurotoxicity in Caenorhabditis elegans

2016 ◽  
Vol 2016 ◽  
pp. 1-10 ◽  
Author(s):  
Haifeng Li ◽  
Ruona Shi ◽  
Fei Ding ◽  
Hongyu Wang ◽  
Wenjing Han ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Caenorhabditis Elegans ◽  
Protective Effect ◽  
Therapeutic Potential ◽  
Neuroprotective Effect ◽  
Acetylcholinesterase Activity ◽  
Apoptosis Pathway ◽  
Acidic Polysaccharide ◽  
C Elegans ◽  
6 Hydroxydopamine

Astragalus membranaceus is a medicinal plant traditionally used in China for a variety of conditions, including inflammatory and neural diseases. Astragalus polysaccharides are shown to reduce the adverse effect of levodopa which is used to treat Parkinson’s disease (PD). However, the neuroprotective effect of Astragalus polysaccharides per se in PD is lacking. Using Caenorhabditis elegans models, we investigated the protective effect of astragalan, an acidic polysaccharide isolated from A. membranaceus, against the neurotoxicity of 6-hydroxydopamine (6-OHDA), a neurotoxin that can induce parkinsonism. We show that 6-OHDA is able to degenerate dopaminergic neurons and lead to the deficiency of food-sensing behavior and a shorter lifespan in C. elegans. Interestingly, these degenerative symptoms can be attenuated by astragalan treatment. Astragalan is also shown to alleviate oxidative stress through reducing reactive oxygen species level and malondialdehyde content and increasing superoxide dismutase and glutathione peroxidase activities and reduce the expression of proapoptotic gene egl-1 in 6-OHDA-intoxicated nematodes. Further studies reveal that astragalan is capable of elevating the decreased acetylcholinesterase activity induced by 6-OHDA. Together, our results demonstrate that the protective effect of astragalan against 6-OHDA neurotoxicity is likely due to the alleviation of oxidative stress and regulation of apoptosis pathway and cholinergic system and thus provide an important insight into the therapeutic potential of Astragalus polysaccharide in neurodegeneration.

scholarly journals AdipoR2 is transcriptionally regulated by ER stress-inducible ATF3 in HepG2 human hepatocyte cells

FEBS Journal ◽  
2010 ◽  
Vol 277 (10) ◽  
pp. 2304-2317 ◽  
Author(s):  
In-uk Koh ◽  
Joo H. Lim ◽  
Myung K. Joe ◽  
Won H. Kim ◽  
Myeong H. Jung ◽  
...  
Keyword(s):  
Er Stress ◽  
Human Hepatocyte ◽  
Hepatocyte Cells

scholarly journals Caenorhabditis elegans to Model the Capacity of Ascorbic Acid to Reduce Acute Nitrite Toxicity under Different Feed Conditions: Multivariate Analytics on Behavioral Imaging

2021 ◽  
Vol 18 (4) ◽  
pp. 2068
Author(s):  
Samuel Verdu ◽  
Alberto J. Perez ◽  
Conrado Carrascosa ◽  
José M. Barat ◽  
Pau Talens ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Caenorhabditis Elegans ◽  
Protective Effect ◽  
Animal Health ◽  
Biological Systems ◽  
Meat Products ◽  
Processed Meat ◽  
Multivariate Statistical ◽  
C Elegans ◽  
Nitrite Toxicity

Nitrocompounds are present in the environment and human diet and form part of vegetables and processed meat products as additives. These compounds are related to negative impacts on human and animal health. The protective effect of ascorbic acid has been demonstrated by some biological systems as regards several nitrocompounds. This work focused on studying the possibility of modeling this effect on nitrite toxicity with the model Caenorhabditis elegans. The three factors studied in this work were ascorbic acid concentration, nitrite exposure concentration, and presence/absence of food. The protective effect was evaluated by scoring lethality and its impact on behavior by means of multivariate statistical methods and imaging analytics. The effects of nitrite and the influence of food availability were evidenced. Apart from increasing lethality, nitrite had disruption effects on movements. All the observed symptoms reduced when ascorbic acid was administered, and it diminished lethality in all cases. Ascorbic acid maintained nematodes’ postural capacities. The results suggest that nitrites’ nonspecific toxicity in C. elegans can be mitigated by ascorbic acid, as previously evidenced in other biological systems. Thus, our results reveal the ability of C. elegans to reproduce the known protective effect of ascorbic acid against nitrite.

scholarly journals Ingredients in Zijuan Pu’er Tea Extract Alleviate β-Amyloid Peptide Toxicity in a Caenorhabditis elegans Model of Alzheimer’s Disease Likely through DAF-16

Molecules ◽  
2019 ◽  
Vol 24 (4) ◽  
pp. 729 ◽  
Author(s):  
Fangzhou Du ◽  
Lin Zhou ◽  
Yan Jiao ◽  
Shuju Bai ◽  
Lu Wang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Caenorhabditis Elegans ◽  
Protective Effect ◽  
Nuclear Translocation ◽  
Amyloid Β ◽  
Amyloid Peptide ◽  
C Elegans ◽  
Β Amyloid

Amyloid-β, one of the hallmarks of Alzheimer’s disease (AD), is toxic to neurons and can also cause brain cell death. Oxidative stress is known to play an important role in AD, and there is strong evidence that oxidative stress is associated with amyloid-β. In the present study we report the protective effect of Zijuan Pu’er tea water extract (ZTWE) and the mixture of main ingredients (+)-catechins, caffeine and procyanidin (MCCP) in ZTWE on β-amyloid-induced toxicity in transgenic Caenorhabditis elegans (C. elegans) CL4176 expressing the human Aβ1–42 gene. ZTWE, (+)-catechins, caffeine, procyanidin and MCCP delayed the β-amyloid-induced paralysis to different degrees. The MCCP treatment did not affect the transcript abundance of amyloid-β transgene (amy-1); however, Thioflavin T staining showed a significant decrease in Aβ accumulation compared to untreated worms. Further research using transgenic worms found that MCCP promoted the translocation of DAF-16 from cytoplasm to nucleus and increased the expression of superoxide dismutase 3 (SOD-3). In addition, MCCP decreased the reactive oxygen species (ROS) content and increased the SOD activity in CL4176 worms. In conclusion, the results suggested that MCCP had a significant protective effect on β-amyloid-induced toxicity in C. elegans by reducing β-amyloid aggregation and inducing DAF-16 nuclear translocation that could activate the downstream signal pathway and enhance resistance to oxidative stress.

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