An immune-enriched oligo-microarray analysis of gene expression in Manila clam (Venerupis philippinarum) haemocytes after a Perkinsus olseni challenge

In this study, we verified the effects of 2-aza-8-oxohypoxanthine (AOH) on human epidermal cell proliferation by performing DNA microarray analysis. Cell proliferation was assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, which measures mitochondrial respiration in normal human epidermal keratinocyte (NHEK) cells. Gene expression levels were determined by DNA microarray analysis of 177 genes involved in skin aging and disease. AOH showed a significant increase in cell viability at concentrations between 7.8 and 31.3 μg/mL and a significant decrease at concentrations above 250 μg/mL. DNA microarray analysis showed that AOH significantly increased the gene expression of CLDN1, DSC1, DSG1, and CDH1 (E-cadherin), which are involved in intercellular adhesion and skin barrier functioning. AOH also up-regulated the expression of KLK5, KLK7, and SPIMK5, which are proteases involved in stratum corneum detachment. Furthermore, AOH significantly stimulated the expression of KRT1, KRT10, TGM1, and IVL, which are considered general differentiation indicators, and that of SPRR1B, a cornified envelope component protein. AOH exerted a cell activation effect on human epidermal cells. Since AOH did not cause cytotoxicity, it was considered that the compound had no adverse effects on the skin. In addition, it was found that AOH stimulated the expression levels of genes involved in skin barrier functioning by DNA microarray analysis. Therefore, AOH has the potential for practical use as a cosmetic ingredient. This is the first report of efficacy evaluation tests performed for AOH.

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GW26-e0264 Microarray analysis of differential gene expression profile in the peripheral blood cells of the patients with myocardial infarction

Journal of the American College of Cardiology ◽

10.1016/j.jacc.2015.06.596 ◽

2015 ◽

Vol 66 (16) ◽

pp. C155-C156

Author(s):

Xudong Guo ◽

Lin Fan ◽

Xiangdong Li ◽

Fanbo Meng

Keyword(s):

Gene Expression ◽

Myocardial Infarction ◽

Gene Expression Profile ◽

Differential Gene Expression ◽

Microarray Analysis ◽

Expression Profile ◽

Peripheral Blood ◽

Blood Cells ◽

Peripheral Blood Cells ◽

Differential Gene

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Sulforaphane Enhances the Ability of Human Retinal Pigment Epithelial Cell against Oxidative Stress, and Its Effect on Gene Expression Profile Evaluated by Microarray Analysis

Oxidative Medicine and Cellular Longevity ◽

10.1155/2013/413024 ◽

2013 ◽

Vol 2013 ◽

pp. 1-13 ◽

Cited By ~ 10

Author(s):

Liang Ye ◽

Ting Yu ◽

Yanqun Li ◽

Bingni Chen ◽

Jinshun Zhang ◽

...

Keyword(s):

Oxidative Stress ◽

Gene Expression ◽

Microarray Analysis ◽

Retinal Pigment Epithelial ◽

Nuclear Translocation ◽

Retinal Pigment ◽

Regulation Of Gene Expression ◽

Age Related Macular Degeneration ◽

Rpe Cells ◽

Pigment Epithelial

To gain further insights into the molecular basis of Sulforaphane (SF) mediated retinal pigment epithelial (RPE) 19 cell against oxidative stress, we investigated the effects of SF on the regulation of gene expression on a global scale and tested whether SF can endow RPE cells with the ability to resist apoptosis. The data revealed that after exposure to H2O2, RPE 19 cell viability was increased in the cells pretreated with SF compared to the cell not treated with SF. Microarray analysis revealed significant changes in the expression of 69 genes in RPE 19 cells after 6 hours of SF treatment. Based on the functional relevance, eight of the SF-responsive genes, that belong to antioxidant redox system, and inflammatory responsive factors were validated. The up-regulating translation of thioredoxin-1 (Trx1) and the nuclear translocation of Nuclear factor-like2 (Nrf2) were demonstrated by immunoblot analysis in SF treated RPE cells. Our data indicate that SF increases the ability of RPE 19 cell against oxidative stress through up-regulating antioxidative enzymes and down-regulating inflammatory mediators and chemokines. The results suggest that the antioxidant, SF, may be a valuable supplement for preventing and retarding the development of Age Related Macular Degeneration.

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