Litopenaeus vannamei sirtuin 6 homolog (LvSIRT6) is involved in immune response by modulating hemocytes ROS production and apoptosis

ABSTRACTThe objectives of this study were immune response, specific growth rate and survival rate of shrimp vaname (Litopenaeus vannamei) in ponds. The research method used was experimental to know the effect of feed use and added crude protein Zoothamnium penaei on vaname shrimp (Lithopenaeus vannamei) in pond. The sample used is shrimp vaname (Lithopenaeus vannamei) as much as 10,000 heads of juvenile stadia. The food used is commercial feed added crude protein Zoothamnium penaei with a dose of 150 μl / head, which is given 7 times every 7 days interval from the age of 1 day up to Shrimp aged 56 days in ponds. The results showed that there was an increase of immune response (increase of THC and DHC) due to feeding added by Zoothamnium penaei crude. The highest total of Haemocytes (THC) occurred in commercial-fed shrimp and added crude protein ie 56,58 x 106 cell / ml, And the lowest in shrimp shrimp that is not given its crude protein 23.57 x 106 cells / ml. Similarly, the highest Differential Haemosite Count (DHC) also occurred in shrimps fed commercial and added crude protein, 26.57% aged 60 days in ponds and 14.99% low on shrimp not given 90 day crude protein in ponds . Results of parasite shrimp infestation examination exposed with crude protein showed that the highest Zoothamnium penaei infestation was obtained in shrimp fed with artificial feed and not added crude protein Zoothamnium penaei that is 63,35% in shrimp age 90 days. While the shrimp were given artificial feed and added crude protein Zoothamnium penaei infestation Zoothamnium penaei highest of 14.27% in 90 days old shrimp.The highest specific rate of vaname shrimp growth also occurred in shrimp fed commercial and added crude protein Zoothamnium penaei highest of 53.46% and the lowest 16.15% in shrimps aged 90 days in ponds. The highest shrimp life occurrence occurred in commercial vaname shrimp and added crude protein Zoothamnium penaei age of 90 days which has a higher tendency than shrimp that is not given crude protein that is 72% and 21%. The addition of Zoothamnium crude protein to commercial feed as immunostimulant material may enhance immune response, specific growth and shelf life of 30, 60 and 90 days old shrimp in ponds, so that it can be developed as an immunostimulant material.Keyword: Litopenaeus Vannamei, Zoothamnium penaei, udang vanamei

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Exopolysaccharide from Porphyridium cruentum (purpureum) is Not Toxic and Stimulates Immune Response against Vibriosis: The Assessment Using Zebrafish and White Shrimp Litopenaeus vannamei

10.20944/preprints202102.0422.v1 ◽

2021 ◽

Author(s):

Yenny Risjani ◽

Nurul Mutmainnah ◽

Praprianita Manurung ◽

Siti Wulan ◽

Yunianta Yunianta

Keyword(s):

Immune Response ◽

Litopenaeus Vannamei ◽

Porphyridium Cruentum ◽

White Shrimp ◽

Treatment Result ◽

Extracellular Polysaccharides ◽

Mortality Data ◽

Protective Agent ◽

Immune Parameters ◽

Vibrio Infection

Exopolysaccharides or extracellular polysaccharides (EPS, sPS) represent valuable metabolite compound synthesized from red microalgae. It is a non toxic natural agent and can be applied as immunostimulant. Toxicity test of exopolysaccharides from Porphyridium has been done in-vivo using zebrafish (Danio rerio) embryonic model, or the ZET (Zebrafish Embryotoxicity Test). The administration of extracellular polysaccharide or exopolysaccharides (EPS) from microalgae Porphyridium cruentum (synonym: P. purpureum) on shrimps Litopenaeus vannamei was investigated to determine the effect of this immunostimulant on their non specific immune response and to test if this compound can be used as a protective agent for shrimp related to Vibrio infection. For immune response, exopolysaccharides was given to shrimps by immersion method on day 1 and booster on day 8. Shrimp hemocytes were taken on day 1 (EPS administration), day 7 (no treatment), day 8 (EPS booster) and day 9 (Vibrio infection) and tested for their immune response on each treatment. Result shows that the EPS is not toxic as represented by the normal embryonic development and the mortality data. In the Pacific whiteshrimps, it show an increase in values of all immune parameters in line with the increasing EPS concentration, except the Differential Haemocyte Count (DHC). In detail, an increase was noted in total hemocytes (THC) value, Phagocytotic Activity (PA), Respiratory Burst (RB) in line with the EPS concentration increase. These results and other previous studies indicate that EPS from Porphyridium is safe and it enhances immune parameters in shrimp rapidly and has the ability as an immunostimulant or an immunomodulator. It is a good modulator for the non-specific immune cells of Pacific white shrimps, and it can be used as a preventive agent against vibriosis.

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Physiological and immune response of Litopenaeus vannamei undergoing the acute phase of the necrotizing hepatopancreatitis disease and after being treated with oxytetracycline and FF

Latin American Journal of Aquatic Research ◽

10.3856/vol44-issue3-fulltext-12 ◽

2017 ◽

Vol 44 (3) ◽

pp. 535-545

Author(s):

Luis R. Martínez-Córdova ◽

Teresa Gollas-Galván ◽

Estefanía Garibay-Valdez ◽

Rocío Valenzuela-Gutiérrez ◽

Marcel Martínez Porchas ◽

...

Keyword(s):

Immune Response ◽

Immune Responses ◽

Acute Phase ◽

Litopenaeus Vannamei ◽

Positive Control ◽

Negative Control ◽

Negative Effect ◽

Clotting Protein ◽

Immune Related Genes ◽

Shock Proteins

The physiological and immune responses of adult shrimp (Litopenaeus vannamei) undergoing the acute phase of the necrotizing hepatopancreatitis (NHP) disease and the efficiency of oxytetracycline (OXI) and florfenicol (FF) to eliminate the pathogen were evaluated. Four shrimp groups were considered: three groups infected with necrotizing hepatopancreatitis bacteria (NHP-B) (two treated with antibiotics and a positive control) and one group non-infected (negative control). Hemolymph concentration of glucose, lactate, acylglycerides, cholesterol, total protein, aminotransferases, superoxide dismutase, and the transcriptional expression of several immune related genes were monitored at the acute phase of the disease, and at 15 and 20 days after administration of antibiotics (daa). Shrimp from the positive control registered a mortality of 100%. NHP-B infection affected the immu-nophysiological response of shrimp; herein, most of the parameters were significantly up regulated in infected shrimp before the use of antibiotics, compared to the negative control. Increased transcriptional levels of clotting protein, lipopolysaccharide and β-1-3-glucan binding protein (LGBP), serine protease, peroxinectin, lysozyme, heat shock proteins (HSP) 60 and 70 were detected in shrimp treated with OXI. At 20 daa NHP-B was still detected in FF-treated shrimp, but not in OXI-treated shrimp. It is hypothesized that despite none of the antibiotics per se eliminated the bacterium, both had a negative effect on its virulence. OXI seems to have a greater effect, allowing shrimp to integrate a better immune response at 15 daa.

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