Lipid accumulation for biodiesel production by oleaginous fungus Aspergillus awamori: Influence of critical factors

Fuel ◽  
2014 ◽  
Vol 116 ◽  
pp. 509-515 ◽  
Author(s):  
G. Venkata Subhash ◽  
S. Venkata Mohan
Keyword(s):  
Lipid Accumulation ◽  
Biodiesel Production ◽  
Aspergillus Awamori ◽  
Critical Factors ◽  
Oleaginous Fungus

Evaluating the effect of medium composition and fermentation conditions on microbial oil production by a Fusarium strain isolated from the open ocean

2017 ◽  
Vol 60 (4) ◽  
Author(s):  
Tian-Hua Zhong ◽  
Jia-Wei Zhang ◽  
Wei Xu ◽  
Liying Huang ◽  
Zhu-Hua Luo
Keyword(s):  
Cell Growth ◽  
Lipid Accumulation ◽  
Oil Production ◽  
Culture Conditions ◽  
Medium Composition ◽  
Open Ocean ◽  
Biodiesel Production ◽  
Microbial Oil ◽  
Fermentation Conditions ◽  
Oleaginous Fungus

AbstractAs an important feedstock for biodiesel production, microbial oil has attracted considerable interest in recent years. Here, we investigated the effect of medium composition and culture conditions on cell growth and lipid accumulation of an oleaginous fungus

scholarly journals Effects of nitrogen, phosphorus, EDTA and sodium chloride on biomass and lipid accumulation of Chaetomorpha aerea

2020 ◽  
pp. 152-158
Author(s):  
Gour Gopal Satpati ◽  
Ruma Pal
Keyword(s):  
Fatty Acids ◽  
Sodium Chloride ◽  
Total Lipid ◽  
Lipid Accumulation ◽  
Saturated Fatty Acids ◽  
Stress Conditions ◽  
Biodiesel Production ◽  
Cultural Conditions ◽  
Chaetomorpha Aerea ◽  
Nitrogen Phosphorus

The increase of total lipid and fatty acids production was studied under different nutrient stress conditions using the macroalga, Chaetomorpha aerea. The effects of nitrogen, phosphorus, ethylene diamine tetra-acetate and sodium chloride on the growth and lipid accumulation were systematically investigated in laboratory conditions. The biomass was harvested at different stages of cultivation and assessed. The maximum changes of growth and lipid accumulation were observed in the exponential phase at different cultural conditions. The two-fold increase of total lipid was found in the order of 28.27±0.04 % (at 0.1 g/L nitrogen) > 27.30±0.37 % (at 0.5 g/L of phosphorus) > 25.86±0.77 % (at 0.05 g/L of EDTA)> 24.37±0.04 % (at 0.05 g/L NaCl) on 8th day of cultivation. The fatty acids were identified and quantified by gas chromatography mass spectrometry (GC-MS). The alga produces significantly high amount of monounsaturated fatty acid (MUFA) and saturated fatty acids (SFA) than the polyunsaturated fatty acids (PUFA) in different cultural conditions. The elevated levels of C16:1, C18:1 and C20:1 was identified under nitrate, phosphate and salt stress conditions, which are more suitable for biodiesel production.

scholarly journals Lipid accumulation of Chlorella sp. TLD6B from the Taklimakan Desert under salt stress

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e11525
Author(s):  
Hong Li ◽  
Jun Tan ◽  
Yun Mu ◽  
Jianfeng Gao
Keyword(s):  
Salt Stress ◽  
Fatty Acid ◽  
Lipid Accumulation ◽  
Large Scale ◽  
Fatty Acid Biosynthesis ◽  
High Salinity ◽  
Raw Material ◽  
Biodiesel Production ◽  
Taklimakan Desert ◽  
Acid Biosynthesis

Chlorella has become an important raw material for biodiesel production in recent years, and Chlorella sp. TLD6B, a species with high lipid concentrations and high salt and drought tolerance, has been cultivated on a large scale. To explore the lipid accumulation of Chlorella sp. TLD6B and its relationship to external NaCl concentrations, we performed physiological measurements and genome-wide gene expression profiling under different levels of salt stress. Chlorella sp. TLD6B was able to tolerate high levels of salt stress (0.8 M NaCl addition). Lipid concentrations initially increased and then decreased as salt stress increased and were highest under the addition of 0.2 M NaCl. Comparative transcriptomic analysis revealed that salt stress enhanced the expression of genes related to sugar metabolism and fatty acid biosynthesis (the ACCases BC and BCCP, KAS II, and GPDHs involved in TAG synthesis), thereby promoting lipid accumulation under the addition of 0.2 M NaCl. However, high salinity inhibited cell growth. Expression of three SADs, whose encoded products function in unsaturated fatty acid biosynthesis, was up-regulated under high salinity (0.8 M NaCl addition). This research clarifies the relationship between salt tolerance and lipid accumulation and promotes the utilization of Chlorella sp. TLD6B.

scholarly journals Consortium Growth of Filamentous Fungi and Microalgae: Evaluation of Different Cultivation Strategies to Optimize Cell Harvesting and Lipid Accumulation

Energies ◽  
2020 ◽  
Vol 13 (14) ◽  
pp. 3648
Author(s):  
Savienne M. F. E. Zorn ◽  
Cristiano E. R. Reis ◽  
Messias B. Silva ◽  
Bo Hu ◽  
Heizir F. De Castro
Keyword(s):  
Lipid Accumulation ◽  
Unsaturated Fatty Acids ◽  
Lipid Production ◽  
Synthetic Medium ◽  
Fungal Spores ◽  
Dry Weight ◽  
Biodiesel Production ◽  
Biomass Composition ◽  
Fungal Mycelium ◽  
Fourfold Increase

This study aims to evaluate the potential of consortium biomass formation between Mucor circinelloides, an oleaginous filamentous fungal species, and Chlorella vulgaris, in order to promote a straightforward approach to harvest microalgal cells and to evaluate the lipid production in the consortium system. A synthetic medium with glucose (2 g·L−1) and mineral nutrients essential for both fungi and algae was selected. Four different inoculation strategies were assessed, considering the effect of simultaneous vs. separate development of fungal spores and algae cells, and the presence of a supporting matrix aiming at the higher recovery of algae cell rates. The results were evaluated in terms of consortium biomass composition, demonstrating that the strategy using a mature fungal mycelium with a higher algae count may provide biomass samples with up to 79% of their dry weight as algae, still promoting recovery rates greater than 97%. The findings demonstrate a synergistic effect on the lipid accumulation by the fungal strain, at around a fourfold increase when compared to the axenic control, with values in the range of 23% of dry biomass weight. Furthermore, the fatty acid profile from the samples presents a balance between saturated and unsaturated fatty acids that is likely to present an adequate balance for applications such as biodiesel production.

13 C-metabolic flux analysis of lipid accumulation in the oleaginous fungus Mucor circinelloides

2015 ◽  
Vol 197 ◽  
pp. 23-29 ◽  
Author(s):  
Lina Zhao ◽  
Huaiyuan Zhang ◽  
Liping Wang ◽  
Haiqin Chen ◽  
Yong Q. Chen ◽  
...  
Keyword(s):  
Metabolic Flux Analysis ◽  
Lipid Accumulation ◽  
Metabolic Flux ◽  
Mucor Circinelloides ◽  
Flux Analysis ◽  
Oleaginous Fungus
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