Blue light promotes bactericidal action of plasma-activated water against Staphylococcus aureus on stainless steel surfaces

2021 ◽  
Vol 69 ◽  
pp. 102663
Author(s):  
Ki Ho Baek ◽  
Hyun-Jun Kim ◽  
Taemin Kang ◽  
Yee Eun Lee ◽  
Do-Kyun Kim ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Stainless Steel ◽  
Blue Light ◽  
Bactericidal Action ◽  
Steel Surfaces ◽  
Plasma Activated Water

scholarly journals STUDY OF THE INFLUENCE OF SAVINASE®EVITY16L ENZYME ON BIOFILMS FORMATION OF STAPHYLOCOCCUS AUREUS ON STAINLESS STEEL WITH DIFFERENT ROUGHNESS

2019 ◽  
Vol 2 ◽  
pp. 26-32 ◽  
Author(s):  
Mykola Kukhtyn ◽  
Khrystyna Kravcheniuk ◽  
Ludmila Beyko ◽  
Yulia Horiuk ◽  
Oleksandr Skliar ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Surface Roughness ◽  
Stainless Steel ◽  
Dairy Products ◽  
Proteolytic Enzyme ◽  
Proteolytic Enzymes ◽  
Dairy Production ◽  
Steel Surfaces ◽  
Dairy Equipment ◽  
Biofilm Matrix

Microbial films formation on the dairy equipment creates a serious problem, because they are difficult to eliminate by washing and disinfecting means that results in contaminating dairy products by microorganisms. The aim of the work was to study the influence of Savinase®Evity 16L proteolytic enzyme on the process of destructing biofilms, formed by Staphylococcus aureus on stainless steel with different surface roughness. It has been established, that surface roughness of stainless steel influences the process of Savinase®Evity 16L enzyme penetration in a hollow and prevents the destruction of the biofilm matrix, created by Staphylococcus aureus. It has been revealed, that after the influence of a proteolytic enzyme on Staphylococcus aureus biofilms, created on steel with roughness 0,16±0,018 mcm, the density decreased in 4,0 times (р≤0,05), comparing with a condition before processing. At roughness 0,63±0,087 mcm the density of formed biofilms decreased at the effect of Savinase®Evity 16L in 3,3times (р≤0,05) and the biofilm was characterized as a weak one. At the same time at stainless steel surfaces with roughness 2,68–0,95mcm, the density of biofilms decreased in 2,3–2,1times (р≤0,05), comparing with a condition before processing, and they were characterized as ones of the middle density. It has been also revealed, that the degradation intensity of biofilms under the influence of Savinase®Evity 16L enzyme at roughness 2,68–0,95 mcm was 1,7–1,9 times (р≤0,05) lower than at the surface with roughness 0,16±0,018 mcm. So, the revealed degradation features of a biofilm, created by Staphylococcus aureus at surfaces of stainless steel of different roughness at the influence of Savinase®Evity 16L proteolytic enzyme give a possibility to substantiate the addition of proteolytic enzymes to the composition of washing means for dairy production. It is also offered to process the surface to the roughness no more than 0,63 mcm for producing food steel for raising the effectiveness of biofilms destruction by enzymes and for the sanitary processing.

scholarly journals Adhesion and biofilm formation by Staphylococcus aureus from food processing plants as affected by growth medium, surface type and incubation temperature

2012 ◽  
Vol 48 (4) ◽  
pp. 737-745
Author(s):  
Heloísa Maria Ângelo Jerônimo ◽  
Rita de Cássia Ramos do Egypto Queiroga ◽  
Ana Caroliny Vieira da Costa ◽  
Isabella de Medeiros Barbosa ◽  
Maria Lúcia da Conceição ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Stainless Steel ◽  
Sodium Hypochlorite ◽  
Peracetic Acid ◽  
Food Processing ◽  
Biofilm Formation ◽  
Incubation Temperature ◽  
Growth Media ◽  
Processing Plants ◽  
Steel Surfaces

This study assessed the effect of different growth media [BHI broth, BHI broth plus glucose (10 g/100 mL) and BHI broth plus NaCl (5 g/100 mL)] and incubation temperatures (28 or 37 ºC) on the adherence, detachment and biofilm formation on polypropylene and stainless steel surfaces (2 x 2 cm coupons) for a prolonged period (24-72 h) by some strains of Staphylococcus aureus (S3, S28 and S54) from food processing plants. The efficacy of the sanitizers sodium hypochlorite (250 mg/mL) and peracetic acid (30 mg/mL) in reducing the number of viable bacterial cells in a preformed biofilm was also evaluated. S. aureus strains adhered in highest numbers in BHI broth, regardless of the type of surface or incubation temperature. Cell detachment from surfaces revealed high persistence over the incubation period. The number of cells needed for biofilm formation was noted in all experimental systems after 3 days. Peracetic acid and sodium hypochlorite were not efficient in completely removing the cells of S. aureus adhered onto polypropylene and stainless steel surfaces. From these results, the assayed strains revealed high capacities to adhere and form biofilms on polypropylene and stainless steel surfaces under the different growth conditions, and the cells in biofilm matrixes were resistant to total removal when exposed to the sanitizers sodium hypochlorite and peracetic acid.

scholarly journals Effectiveness of cleaning and sanitizing procedures in controlling the adherence of Pseudomonas fluorescens, Salmonella Enteritidis, and Staphylococcus aureus to domestic kitchen surfaces

2010 ◽  
Vol 30 (1) ◽  
pp. 231-236 ◽  
Author(s):  
Iara Dias Silva ◽  
Roberta Torres Careli ◽  
Júnia Capua Lima ◽  
Nelio José Andrade
Keyword(s):  
Staphylococcus Aureus ◽  
Stainless Steel ◽  
Biofilm Formation ◽  
Salmonella Enteritidis ◽  
Ammonium Compound ◽  
Significant Difference ◽  
Before And After ◽  
Pure Cultures ◽  
Steel Surfaces ◽  
And Staphylococcus Aureus

The effectiveness of cleaning and sanitizing procedures in controlling Staphylococcus aureus, Salmonella Enteritidis, and Pseudomonasfluorescens adhered to granite and stainless steel was evaluated. There was no significant difference (p > 0.05) in the adherence of pure cultures of these microorganisms to stainless steel. The numbers of P. fluorescens and S. Enteritidis adhered to granite were greater (p < 0.05) than the numbers of S. aureus. Additionally, the adherence of P. fluorescens was similar to the adherence of S. Enteritidis on granite surface. In a mixed culture with P. fluorescens, S aureus adhered less (p < 0.05) to stainless steel surfaces (1.31 log CFU.cm-2) than when in a pure culture (6.10 log CFU.cm-2). These results suggest that P. fluorescens inhibited the adherence of S. aureus. However, this inhibition was not observed in the adherence process for granite. There was a significant difference (p < 0.05) between the number of adhered cells before and after pre-washing for S. aureus on stainless steel and granite surfaces, and after washing with detergent for all microorganisms and surfaces. The efficiency of the cleaning plus sanitizing procedures was not significantly different (p > 0.05) between the surfaces. However, a significant difference was observed (p < 0.05) between the sanitizer solutions. Sodium hypochlorite and peracetic acid were more bactericidal (p < 0.05) than a quaternary ammonium compound. With regard to microorganisms, S. aureus was the least resistant to the sanitizers. These results show the importance of good cleaning and sanitization procedures to prevent bacterial adherence and biofilm formation.

"Escherichia coli-milk" Biofilm Removal from Stainless Steel Surfaces: Synergism between Ultrasonic Waves and Enzymes

Biofouling ◽  
2003 ◽  
Vol 19 (3) ◽  
pp. 159-168 ◽  
Author(s):  
Nadia Oulahal- Lagsir ◽  
Adele Martial- Gros ◽  
Marc Bonneauc ◽  
Loic Bluma
Keyword(s):  
Escherichia Coli ◽  
Stainless Steel ◽  
Ultrasonic Waves ◽  
Biofilm Removal ◽  
Steel Surfaces

" Escherichia coli -milk" Biofilm Removal from Stainless Steel Surfaces: Synergism between Ultrasonic Waves and Enzymes

Biofouling ◽  
2003 ◽  
Vol 19 (3) ◽  
pp. 159-168 ◽  
Author(s):  
NADIA OULAHAL-LAGSIR ◽  
ADELE MARTIAL-GROS ◽  
MARC BONNEAU ◽  
LOIC BLUM
Keyword(s):  
Escherichia Coli ◽  
Stainless Steel ◽  
Ultrasonic Waves ◽  
Biofilm Removal ◽  
Steel Surfaces

Use of eugenol and cuminaldehyde for disinfection of stainless steel surfaces

Planta Medica ◽  
2014 ◽  
Vol 80 (16) ◽  
Author(s):  
I Gomes ◽  
J Malheiro ◽  
A Abreu ◽  
A Borges ◽  
F Mergulhão ◽  
...  
Keyword(s):  
Stainless Steel ◽  
Steel Surfaces
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