Extraction of yellow pear residue polysaccharides and effects on immune function and antioxidant activity of immunosuppressed mice

The purpose of this study was to investigate the effects of dietary Selenohomolanthionine (SeHLan) on antioxidant status and immune response in canine parvovirus (CPV) vaccinated puppies. In this study, 30 weaned puppies were randomly divided into six groups: control group (–Se/–Vacc), immunization group (–Se/+Vacc), supplementation of sodium selenite group (SS/+Vacc, 0.35 mg/kg DM), low-dose SeHLan group (SeHLan-L/+Vacc, 0.35 mg/kg DM), mid-dose SeHLan group (SeHLan-M/+Vacc, 1.0 mg/kg DM), and high-dose SeHLan group (SeHLan-H/+Vacc, 2.0 mg/kg DM). The puppies were fed for 42 days and vaccinated with Vanguard Plus 5 on day 0 and day 21. Blood samples were collected on 7, 14, 21, 28, 35, 42 days post-immunization (PI) for determination of antioxidant indicators, lymphocyte proliferation index, serum cytokine concentration (IL-2, IL-4), canine polymorphonuclear neutrophils (PMN) phagocytic function, and the level of CPV antibody titers. The results showed that SeHLan supplementation raised the serum Se concentration and glutathione peroxidase (GSH-Px) activity in a dose-dependent manner (P < 0.05). It also increased the activity of serum superoxide dismutase (SOD) and decreased serum malondialdehyde (MDA) content, especially in SeHLan-M/+Vacc group (1.0 mg/kg DM) (P < 0.01). SeHLan supplementation significantly increased lymphocyte proliferation, IL-2, and IL-4 levels in canine serum, and enhanced phagocytosis of PMN in vaccinated puppies (P < 0.05). Moreover, SeHLan supplementation shortened the CPV antibody production time and increased the CPV antibody titers (P < 0.05). Of note, the beneficial effects of SeHLan were superior to those of SS. In conclusion, dietary SeHLan supplementation improved antioxidant activity, increased CPV antibody titers, and enhanced immune function in puppies after weaning. An appropriate dosage of SeHLan (1~2 mg/kg DM) may confer nutritional benefits in puppies.

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Polysaccharide from natural Cordyceps sinensis ameliorated intestinal injury and enhanced antioxidant activity in immunosuppressed mice

Food Hydrocolloids ◽

10.1016/j.foodhyd.2018.11.018 ◽

2019 ◽

Vol 89 ◽

pp. 661-667 ◽

Cited By ~ 13

Author(s):

Shuping Chen ◽

Junqiao Wang ◽

Qiuyue Fang ◽

Nan Dong ◽

Shaoping Nie

Keyword(s):

Antioxidant Activity ◽

Intestinal Injury ◽

Cordyceps Sinensis ◽

Immunosuppressed Mice

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Pinus koraiensis polyphenols: structural identification, in vitro antioxidant activity, immune function and inhibition of cancer cell proliferation

Food & Function ◽

10.1039/d0fo03347b ◽

2021 ◽

Author(s):

Yuyang Huang ◽

Xiuqing Zhu ◽

Ying Zhu ◽

Zhenyu Wang

Keyword(s):

Cell Proliferation ◽

Antioxidant Activity ◽

Immune Function ◽

Cancer Cell ◽

Structural Identification ◽

Pinus Koraiensis ◽

Cancer Cell Proliferation ◽

In Vitro Antioxidant Activity

Herein, polyphenols were extracted from Pinus koraiensis bark and characterized.

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Protein Utilization, Immune Function, and Hepatic Antioxidant Activity of Syrian Hamster Rats Fed Tahini in Combination with Other Oily Seeds

Nutrition and Food Technology Open Access ◽

10.16966/2470-6086.171 ◽

2021 ◽

Vol 7 (1) ◽

Author(s):

Sumainah G ◽

Laban L

Keyword(s):

Antioxidant Activity ◽

Preschool Children ◽

Immune Function ◽

Syrian Hamster ◽

Sesame Seed ◽

Protein Source ◽

Plant Proteins ◽

Protein Utilization

Background: Consumption of sesame seed products is increasing worldwide, and sesame may be suitable as a quality protein source for preschool children when combined with other sources of plant proteins.

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Protective Effect of Bergenin against Cyclophosphamide-Induced Immunosuppression by Immunomodulatory Effect and Antioxidation in Balb/c Mice

Molecules ◽

10.3390/molecules23102668 ◽

2018 ◽

Vol 23 (10) ◽

pp. 2668 ◽

Cited By ~ 8

Author(s):

Qiuchen Qi ◽

Zhonghua Dong ◽

Yueyue Sun ◽

Siying Li ◽

Zhongxi Zhao

Keyword(s):

Immune Function ◽

Adverse Reactions ◽

Histological Analysis ◽

Lymphocyte Subsets ◽

Immune Functions ◽

Humoral Immune ◽

Immune Organs ◽

Total Antioxidant ◽

Cellular Immune ◽

Immunosuppressed Mice

In this study, the aim was to investigate the effect of bergenin on immune function and antioxidation in cyclophosphamide (Cy)-induced immunosuppressed mice. Firstly, we estimated its effect on immune organs. Histological analysis and indexes of immune organs showed that cyclophosphamide exhibited spleen and thymus injury compared with the normal control, which was alleviated by bergenin. Secondly, bergenin also enhanced the humoral immune function through increasing the level of IgM and IgG in serum. Thirdly, bergenin also enhanced the cellular immune function. The results indicate that bergenin increased peritoneal macrophage functions, the proliferation of T and B lymphocytes, NK and CTL cell activities, and T (CD4+ and CD8+) lymphocyte subsets. Besides, bergenin also had the ability to modulate the Th1/Th2 balance. Moreover, bergenin prevented the Cy-induced decrease in numbers of peripheral RBC, WBC and platelets, providing supportive evidence for their anti-leukopenia activities. Finally, bergenin also reversed the Cy-induced decrease in the total antioxidant capacity including activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px). In conclusion, bergenin protected against Cy-induced adverse reactions by enhancing humoral and cellular immune functions and augmenting antioxidative activity and could be considered as a potential immunomodulatory agent.

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Improvement of cytoprotective and antioxidant activity of Rosa canina L. and Salix alba L. by controlled differential sieving process against H2O2-induced oxidative stress in mouse primary splenocytes

International Journal for Vitamin and Nutrition Research ◽

10.1024/0300-9831/a000506 ◽

2017 ◽

Vol 87 (3-4) ◽

pp. 191-200 ◽

Cited By ~ 1

Author(s):

Nidhal Soualeh ◽

Aliçia Stiévenard ◽

Elie Baudelaire ◽

Rachid Soulimani ◽

Jaouad Bouayed

Keyword(s):

Antioxidant Activity ◽

Antioxidant Activities ◽

Biological Activities ◽

Good Alternative ◽

Activity Levels ◽

Salix Alba ◽

Rosa Canina ◽

Free Process ◽

Plant Powders ◽

Sieving Process

Abstract. In this study, cytoprotective and antioxidant activities of Rosa canina (RC) and Salix alba (SA), medicinal plants, were studied on mouse primary splenocytes by comparing Controlled Differential Sieving process (CDSp), which is a novel green solvent-free process, versus a conventional technique, employing hydroethanolic extraction (HEE). Thus, preventive antioxidant activity of three plant powders of homogeneous particle sizes, 50–100 µm, 100–180 µm and 180–315 µm, dissolved directly in the cellular buffer, were compared to those of hydroethanolic (HE) extract, at 2 concentrations (250 and 500 µg/mL) in H2O2-treated spleen cells. Overall, compared to HE extract, the superfine powders, i. e., fractions < 180 µm, at the lowest concentration, resulted in greater reactive oxygen species (ROS) elimination, increased glutathione peroxidase (GPx) activity and lower malondialdehyde (MDA) production. Better antioxidant and preventive effects in pre-treated cells were found with the superfine powders for SA (i. e., 50–100 µm and 100–180 µm, both p < 0.001), and with the intermediate powder for RC (i. e., 100–180 µm, p < 0.05) versus HE extract. The activity levels of catalase (CAT) and superoxide dismutase (SOD) in pretreated splenocytes exposed to H2O2, albeit reduced, were near to those in unexposed cells, suggesting that pretreatment with the fine powders has relatively restored the normal levels of antioxidant-related enzymes. These findings supported that CDSp improved the biological activities of plants, avoiding the use of organic solvents and thus it could be a good alternative to conventional extraction techniques.

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