A Naringin-loaded gelatin-microsphere/nano-hydroxyapatite/silk fibroin composite scaffold promoted healing of critical-size vertebral defects in ovariectomised rat

One of the merging methods to produce tissue-engineered vascular substitutes is to process scaffolds to direct the regeneration of vascular tissues. Collagen, as one of the main protein in the vascular extracellular matrix, is one of biopolymers that exhibits a major potential for scaffold technology. However, gels made from reconstituted collagen generally exhibit poor mechanical properties and limited manipulability. Therefore, adding a reinforcement to the scaffold to make the structure resist to the physiological constraints applied during the regeneration represents a valid alternative. Silk fibroin is an interesting reinforcing candidate being a mechanically strong natural fibre, susceptible to proteolytic degradation in vivo and showing acceptable biological performances. Therefore, the aim of this study was to develop a model of a composite scaffold obtained by controlling the filament geometry winding of silk fibroin in the collagen gel. A finite element model taking into account the orthotropic elasticity of arteries has been combined with classic laminate theory applied to the filament winding of a tubular vessel. The design of the small structure susceptible to scaffold the vascular tissue regeneration was optimised by mean of an evolutive algorithm with the imperative to mimic the experimentally measured mechanical properties (compliance) of a native artery.

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Performance of a porous composite scaffold containing silk fibroin: Applied research repair on oral jaw epithelial defects

Materials Express ◽

10.1166/mex.2020.1668 ◽

2020 ◽

Vol 10 (4) ◽

pp. 490-502

Author(s):

Huajun Zhu ◽

Chunyu Qian ◽

Wanshu Xiao ◽

Qiang Zhang ◽

Zili Ge

Keyword(s):

Mechanical Properties ◽

Contact Angle ◽

Silk Fibroin ◽

Composite Scaffold ◽

Epithelial Tissue ◽

Porous Composite ◽

Clinical Value ◽

Freeze Dried ◽

Silk Fibroin Film ◽

Oral Epithelial

Application research on repairing oral and maxillofacial epithelial defects with filin-protein porous composite scaffold. The silk fibroin solution was synthesized by hydrothermal synthesis, and the film was prepared by stirring and pouring. Then silk fibroin film and silk fibroin freeze-dried support were prepared by stirring and smooth casting. It was characterized by FTIR, mechanical properties, dissolution detection, contact Angle and SEM. To evaluate the performance of this material in repairing rabbit oral mucosa and rabbit skin epithelial defects. The characterization shows that the material has good contact Angle, mechanical properties, dissolution and biocompatibility. It has good repair function to rabbit oral epithelial tissue and skin epithelial tissue. Silk fibroin has excellent and unique properties. It has good development prospects and great clinical value in tissue regeneration.

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Biomimetic and osteogenic 3D silk fibroin composite scaffolds with nano MgO and mineralized hydroxyapatite for bone regeneration

Journal of Tissue Engineering ◽

10.1177/2041731420967791 ◽

2020 ◽

Vol 11 ◽

pp. 204173142096779

Author(s):

Ziquan Wu ◽

Zhulong Meng ◽

Qianjin Wu ◽

Delu Zeng ◽

Zhengdong Guo ◽

...

Keyword(s):

Bone Regeneration ◽

Bone Tissue ◽

Silk Fibroin ◽

Bone Repair ◽

Composite Scaffold ◽

Bone Tissue Regeneration ◽

Bone Mesenchymal Stem Cells ◽

Differentiation Potential ◽

Composite Effect ◽

Sd Rat

Artificial bioactive materials have received increasing attention worldwide in clinical orthopedics to repair bone defects that are caused by trauma, infections or tumors, especially dedicated to the multifunctional composite effect of materials. In this study, a weakly alkaline, biomimetic and osteogenic, three-dimensional composite scaffold (3DS) with hydroxyapatite (HAp) and nano magnesium oxide (MgO) embedded in fiber (F) of silkworm cocoon and silk fibroin (SF) is evaluated comprehensively for its bone repair potential in vivo and in vitro experiments, particularly focusing on the combined effect between HAp and MgO. Magnesium ions (Mg2+) has long been proven to promote bone tissue regeneration, and HAp is provided with osteoconductive properties. Interestingly, the weak alkaline microenvironment from MgO may also be crucial to promote Sprague-Dawley (SD) rat bone mesenchymal stem cells (BMSCs) proliferation, osteogenic differentiation and alkaline phosphatase (ALP) activities. This SF/F/HAp/nano MgO (SFFHM) 3DS with superior biocompatibility and biodegradability has better mechanical properties, BMSCs proliferation ability, osteogenic activity and differentiation potential compared with the scaffolds adding HAp or MgO alone or neither. Similarly, corresponding meaningful results are also demonstrated in a model of distal lateral femoral defect in SD rat. Therefore, we provide a promising 3D composite scaffold for promoting bone regeneration applications in bone tissue engineering.

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Preparation of silk fibroin/collagen/hydroxyapatite composite scaffold by particulate leaching method

Materials Letters ◽

10.1016/j.matlet.2013.03.130 ◽

2013 ◽

Vol 105 ◽

pp. 189-191 ◽

Cited By ~ 24

Author(s):

Zhao-Li Mou ◽

Li-Min Duan ◽

Xiao-Ni Qi ◽

Zhi-Qi Zhang

Keyword(s):

Silk Fibroin ◽

Composite Scaffold ◽

Hydroxyapatite Composite ◽

Particulate Leaching

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Fabrication of Silk-Hyaluronan Composite as a Potential Scaffold for Tissue Repair

Frontiers in Bioengineering and Biotechnology ◽

10.3389/fbioe.2020.578988 ◽

2020 ◽

Vol 8 ◽

Author(s):

Li-Min Yu ◽

Tao Liu ◽

Yu-Long Ma ◽

Feng Zhang ◽

Yong-Can Huang ◽

...

Keyword(s):

Silk Fibroin ◽

Tissue Repair ◽

Cellular Proliferation ◽

Composite Scaffold ◽

Bioactive Scaffolds ◽

Cell Based Therapy ◽

Nanofibrous Structure ◽

Marrow Mesenchymal Stem Cells ◽

Good Biocompatibility ◽

The Mean

Interest is rapidly growing in the design and preparation of bioactive scaffolds, mimicking the biochemical composition and physical microstructure for tissue repair. In this study, a biomimetic biomaterial with nanofibrous architecture composed of silk fibroin and hyaluronic acid (HA) was prepared. Silk fibroin nanofiber was firstly assembled in water and then used as the nanostructural cue; after blending with hyaluronan (silk:HA = 10:1) and the process of freeze-drying, the resulting composite scaffolds exhibited a desirable 3D porous structure and specific nanofiber features. These scaffolds were very porous with the porosity up to 99%. The mean compressive modulus of silk-HA scaffolds with HA MW of 0.6, 1.6, and 2.6 × 106 Da was about 28.3, 30.2, and 29.8 kPa, respectively, all these values were much higher than that of pure silk scaffold (27.5 kPa). This scaffold showed good biocompatibility with bone marrow mesenchymal stem cells, and it enhanced the cellular proliferation significantly when compared with the plain silk fibroin. Collectively, the silk-hyaluronan composite scaffold with a nanofibrous structure and good biocompatibility was successfully prepared, which deserved further exploration as a biomimetic platform for mesenchymal stem cell-based therapy for tissue repair.

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