Preparation and biological properties of silk fibroin/nano-hydroxyapatite/hyaluronic acid composite scaffold

After an osteosarcoma excision, recurrence and bone defects are significant challenges for clinicians. In this study, the curcumin (Cur) loaded chitosan (CS) nanoparticles (CCNP) encapsulated silk fibroin (SF)/hyaluronic acid esterified by methacrylate (HAMA) (CCNPs-SF/HAMA) hydrogel for the osteosarcoma therapy and bone regeneration was developed by photocuring and ethanol treatment. The micro or nanofibers networks were observed in the CCNPs-SF/HAMA hydrogel. The FTIR results demonstrated that alcohol vapor treatment caused an increase in β-sheets of SF, resulting in the high compression stress and Young’s modulus of CCNPs-SF/HAMA hydrogel. According to the water uptake analysis, SF caused a slight decrease in water uptake of CCNPs-SF/HAMA hydrogel while CCNPs could enhance the water uptake of it. The swelling kinetic results showed that both the CCNPs and the SF increased the swelling ratio of CCNPs-SF/HAMA hydrogel. The accumulative release profile of CCNPs-SF/HAMA hydrogel showed that the release of Cur from CCNPs-SF/HAMA hydrogel was accelerated when pH value was decreased from 7.4 to 5.5. Besides, compared with CCNPs, the CCNPs-SF/HAMA hydrogel had a more sustainable drug release, which was beneficial for the long-term treatment of osteosarcoma. In vitro assay results indicated that CCNPs-SF/HAMA hydrogel with equivalent Cur concentration of 150 μg/mL possessed both the effect of anti-cancer and promoting the proliferation of osteoblasts. These results suggest that CCNPs-SF/HAMA hydrogel with superior physical properties and the bifunctional osteosarcoma therapy and bone repair may be an excellent candidate for local cancer therapy and bone regeneration.

Download Full-text

Hybrid nanostructured hydroxyapatite–chitosan composite scaffold: bioinspired fabrication, mechanical properties and biological properties

Journal of Materials Chemistry B ◽

10.1039/c5tb00175g ◽

2015 ◽

Vol 3 (23) ◽

pp. 4679-4689 ◽

Cited By ~ 50

Author(s):

Ya-Ping Guo ◽

Jun-Jie Guan ◽

Jun Yang ◽

Yang Wang ◽

Chang-Qing Zhang ◽

...

Keyword(s):

Mechanical Properties ◽

Tissue Engineering ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Composite Scaffold ◽

Biological Properties ◽

Chitosan Composite

A bioinspired strategy has been developed to fabricate a hybrid nanostructured hydroxyapatite–chitosan composite scaffold for bone tissue engineering.

Download Full-text

Fabrication and Characterization of Vitamin E TPGS Loaded Silk Fibroin/Hyaluronic Acid Nanofibrous Scaffolds

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.721.274 ◽

2013 ◽

Vol 721 ◽

pp. 274-277

Author(s):

Li Li Ji ◽

Qiao Ling Li ◽

Zeng Hu Yang ◽

Wei Jing Hu ◽

Kui Hua Zhang

Keyword(s):

Hyaluronic Acid ◽

Vitamin E ◽

Silk Fibroin ◽

Ethanol Vapor ◽

Random Coil ◽

Burst Release ◽

Nanofibrous Scaffolds ◽

Vitamin E Tpgs ◽

Polyethylene Glycol 1000 ◽

Β Sheet

Vitamin E d-alpha-tocopheryl polyethylene glycol 1000 succinate (VE TPGS) loaded silk fibroin (SF)/ hyaluronic acid (HA) nanofibrous scaffolds were fabricated by means of electrospinning to biomimic the natural extracellular matrix. Scanning electronic microscopy (SEM) results indicated that electrospun VE TPGS loaded SF/HA nanofibers were ribbon-shaped, the width of nanofibers decreased slightly with the addition of VE TPGS to SF/HA blended solutions. Fourier transform infrared (FTIR) spectroscopy and Wide-angle X-ray diffraction (WAXD) curves revealed that VE TPGS did not induce SF conformation from random coil to β-sheet. SF conformation converted from random coil to β-sheet after being treated with 75% ethanol vapor. In vitro release studies confirmed VE TPGS had no obvious burst release and present good release behavior.

Download Full-text

PTMAc-PEG-PTMAc hydrogel modified by RGDC and hyaluronic acid promotes neural stem cells' survival and differentiation in vitro

RSC Advances ◽

10.1039/c7ra06614g ◽

2017 ◽

Vol 7 (65) ◽

pp. 41098-41104 ◽

Cited By ~ 6

Author(s):

Ruirui Yang ◽

Caixia Xu ◽

Tao Wang ◽

Yuanqi Wang ◽

Jingnan Wang ◽

...

Keyword(s):

Stem Cells ◽

Central Nervous System ◽

Nervous System ◽

Hyaluronic Acid ◽

Neural Stem Cells ◽

Biological Properties ◽

Central Nervous System Injury ◽

Bioactive Molecules

The enhancement of the biological properties of hydrogels by surface modifying with bioactive molecules is of great significance, especially for the treatment of central nervous system injury by combining engrafted cells.

Download Full-text

Improving physical and biological properties of nylon monofilament as suture by Chitosan/Hyaluronic acid

International Journal of Biological Macromolecules ◽

10.1016/j.ijbiomac.2020.08.081 ◽

2020 ◽

Vol 164 ◽

pp. 3394-3402

Author(s):

Hassan Mohammadi ◽

Farzaneh Alihosseini ◽

Seyed Abdolkarim Hosseini

Keyword(s):

Hyaluronic Acid ◽

Biological Properties

Download Full-text

In vivo performance of electrospun tubular hyaluronic acid/collagen nanofibrous scaffolds for vascular reconstruction in the rabbit model

Journal of Nanobiotechnology ◽

10.1186/s12951-021-01091-0 ◽

2021 ◽

Vol 19 (1) ◽

Author(s):

Yuqing Niu ◽

Massimiliano Galluzzi ◽

Ming Fu ◽

Jinhua Hu ◽

Huimin Xia

Keyword(s):

Hyaluronic Acid ◽

Carotid Artery ◽

Structural Integrity ◽

Composite Scaffold ◽

Vascular Endothelial Cells ◽

Rabbit Model ◽

Vascular Reconstruction ◽

Vascular Prostheses ◽

Nanofibrous Scaffolds

AbstractOne of the main challenges of tissue-engineered vascular prostheses is restenosis due to intimal hyperplasia. The aim of this study is to develop a material for scaffolds able to support cell growth while tolerating physiological conditions and maintaining the patency of carotid artery model. Tubular hyaluronic acid (HA)-functionalized collagen nanofibrous composite scaffolds were prepared by sequential electrospinning method. The tubular composite scaffold has well-controlled biophysical and biochemical signals, providing a good matrix for the adhesion and proliferation of vascular endothelial cells (ECs), but resisting to platelets adhesion when exposed to blood. Carotid artery replacement experiment from 6-week rabbits showed that the HA/collagen nanofibrous composite scaffold grafts with endothelialization on the luminal surface could maintain vascular patency. At retrieval, the composite scaffold maintained good structural integrity and had comparable mechanical strength as the native artery. This study indicating that electrospun scaffolds combined with cells may become an alternative to prosthetic grafts for vascular reconstruction. Graphical Abstract

Download Full-text

Design of a Collagen/Silk Mechano-Compatible Composite Scaffold for the Vascular Tissue Engineering: Focus on Compliance

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.334-335.1169 ◽

2007 ◽

Vol 334-335 ◽

pp. 1169-1172 ◽

Cited By ~ 3

Author(s):

Frédéric Couet ◽

Navneeta Rajan ◽

Simone Vesentini ◽

D. Mantovani

Keyword(s):

Mechanical Properties ◽

Silk Fibroin ◽

Vascular Tissue ◽

Composite Scaffold ◽

Collagen Gel ◽

Element Model ◽

Natural Fibre ◽

Filament Winding ◽

Laminate Theory

One of the merging methods to produce tissue-engineered vascular substitutes is to process scaffolds to direct the regeneration of vascular tissues. Collagen, as one of the main protein in the vascular extracellular matrix, is one of biopolymers that exhibits a major potential for scaffold technology. However, gels made from reconstituted collagen generally exhibit poor mechanical properties and limited manipulability. Therefore, adding a reinforcement to the scaffold to make the structure resist to the physiological constraints applied during the regeneration represents a valid alternative. Silk fibroin is an interesting reinforcing candidate being a mechanically strong natural fibre, susceptible to proteolytic degradation in vivo and showing acceptable biological performances. Therefore, the aim of this study was to develop a model of a composite scaffold obtained by controlling the filament geometry winding of silk fibroin in the collagen gel. A finite element model taking into account the orthotropic elasticity of arteries has been combined with classic laminate theory applied to the filament winding of a tubular vessel. The design of the small structure susceptible to scaffold the vascular tissue regeneration was optimised by mean of an evolutive algorithm with the imperative to mimic the experimentally measured mechanical properties (compliance) of a native artery.

Download Full-text