Colistin resistance prevalence in Escherichia coli from domestic animals in intensive breeding farms of Jiangsu Province

AbstractThe global dissemination of colistin resistance has received a great deal of attention. Recently, the plasmid-mediated colistin resistance encoded by mcr-1 and mcr-2 genes in Escherichia coli (E.coli) strains from animals, food, and patients in China have been reported continuously. To make clear the colisin resistance and mcr gene spread in domestic animals in Jiangsu Province, we collected fecael swabs from pigs, chicken and cattle at different age distributed in intensive feeding farms. The selected chromogenic agar and mcr-PCR were used to screen the colisin resistance and mcr gene carriage. Colistin resistant E.coli colonies were identified from 54.25 % (440/811) pig faecal swabs, from 35.96 % (443/1232) chicken faecal swabs, and 26.92 % (42/156) from cattle faecal swabs. Of all the colisin resistant E.coli colonies, the positive amplifications of mcr-1 were significantly higher than mcr-2. The mcr-1 prevalence was 68.86 % (303/440) in pigs, 87.58 % (388/443) in chicken, and 71.43 % (30/42), compared with 46.82 % (206/440) in pigs, 14.90 % (66/443) in chicken, and 19.05 % (8/42) in cattle of prevalence of mcr-2. Co-occurrence of mcr-1 and mcr-2 was identified in 20 % (88/440) in pigs, 7.22 % (32/443) in chickens, and in 9.52 % (4/42) cattle. These data indicate that mcr was the most important colistin resistance mechanism. Interventions and alternative options are necessary to minimise further dissemination of mcr between food-producing animals and human.IMPORTANCEColistin is recognized one of the last defence lines for the treatment of highly resistant bacteria, but the emergence of resistance that conferred by a transferable plasmid-mediated mcr genes to this vital antibiotic is extremely disturbing. Here, we used E. coli as an index to monitor drug resistance in domestic animals (pigs, chicken and cattle). It was found that the colistin resistance widely occurred at all ages of domestic animals and the mcr-dependent mechanism dominated in E.coli. We also found that the elder and adult animals were a reservoir of resistant strains, suggesting a potential food safety issue and greater public health problems.

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Diverse Escherichia coli lineages from domestic animals carrying colistin resistance gene mcr-1 in an Ecuadorian household

Journal of Global Antimicrobial Resistance ◽

10.1016/j.jgar.2019.12.002 ◽

2020 ◽

Vol 22 ◽

pp. 63-67 ◽

Cited By ~ 1

Author(s):

Fernanda Loayza-Villa ◽

Liseth Salinas ◽

Nathalie Tijet ◽

Fernando Villavicencio ◽

Rafael Tamayo ◽

...

Keyword(s):

Escherichia Coli ◽

Resistance Gene ◽

Domestic Animals ◽

Colistin Resistance

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Decreased colistin resistance and mcr-1 prevalence in pig-derived Escherichia coli in Japan after banning colistin as a feed additive

Journal of Global Antimicrobial Resistance ◽

10.1016/j.jgar.2021.01.016 ◽

2021 ◽

Vol 24 ◽

pp. 383-386

Author(s):

Masaru Usui ◽

Yohei Nozawa ◽

Akira Fukuda ◽

Tomomi Sato ◽

Michi Yamada ◽

...

Keyword(s):

Escherichia Coli ◽

Feed Additive ◽

Colistin Resistance

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Tracing the origins of SARS-COV-2 in coronavirus phylogenies: a review

Environmental Chemistry Letters ◽

10.1007/s10311-020-01151-1 ◽

2021 ◽

Vol 19 (2) ◽

pp. 769-785 ◽

Cited By ~ 1

Author(s):

Erwan Sallard ◽

José Halloy ◽

Didier Casane ◽

Etienne Decroly ◽

Jacques van Helden

Keyword(s):

Sequence Analysis ◽

Structure Function ◽

Molecular Mechanisms ◽

Laboratory Strain ◽

Domestic Animals ◽

Scientific Policy ◽

Human Coronavirus ◽

Viral Dissemination ◽

Link Type ◽

Intensive Breeding

AbstractSARS-CoV-2 is a new human coronavirus (CoV), which emerged in China in late 2019 and is responsible for the global COVID-19 pandemic that caused more than 97 million infections and 2 million deaths in 12 months. Understanding the origin of this virus is an important issue, and it is necessary to determine the mechanisms of viral dissemination in order to contain future epidemics. Based on phylogenetic inferences, sequence analysis and structure–function relationships of coronavirus proteins, informed by the knowledge currently available on the virus, we discuss the different scenarios on the origin—natural or synthetic—of the virus. The data currently available are not sufficient to firmly assert whether SARS-CoV2 results from a zoonotic emergence or from an accidental escape of a laboratory strain. This question needs to be solved because it has important consequences on the risk/benefit balance of our interactions with ecosystems, on intensive breeding of wild and domestic animals, on some laboratory practices and on scientific policy and biosafety regulations. Regardless of COVID-19 origin, studying the evolution of the molecular mechanisms involved in the emergence of pandemic viruses is essential to develop therapeutic and vaccine strategies and to prevent future zoonoses. This article is a translation and update of a French article published in Médecine/Sciences, August/September 2020 (10.1051/medsci/2020123).

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Genomic Characterization of New Variant of Hydrogen Sulfide (H2S)-Producing Escherichia coli with Multidrug Resistance Properties Carrying the mcr-1 Gene in China

Antibiotics ◽

10.3390/antibiotics9020080 ◽

2020 ◽

Vol 9 (2) ◽

pp. 80 ◽

Cited By ~ 7

Author(s):

Silpak Biswas ◽

Mohammed Elbediwi ◽

Guimin Gu ◽

Min Yue

Keyword(s):

Escherichia Coli ◽

Hydrogen Sulfide ◽

Multidrug Resistance ◽

Bacterial Infections ◽

Multidrug Resistant ◽

Health Concern ◽

Colistin Resistance ◽

New Variant ◽

Genomic Characterization ◽

Human Infections

Colistin is considered to be a ‘last-resort’ antimicrobial for the treatment of multidrug-resistant Gram-negative bacterial infections. Identification of Enterobacteriaceae, carrying the transferable colistin resistance gene mcr-1, has recently provoked a global health concern. This report presents the first detection of a hydrogen sulfide (H2S)-producing Escherichia coli variant isolated from a human in China, with multidrug resistance (MDR) properties, including colistin resistance by the mcr-1 gene, which could have great implications for the treatment of human infections.

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Emergence of Plasmid-Mediated Colistin Resistance Genemcr-1in a Clinical Escherichia coli Isolate from Egypt

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.00269-16 ◽

2016 ◽

Vol 60 (5) ◽

pp. 3249-3250 ◽

Cited By ~ 57

Author(s):

Shimaa S. Elnahriry ◽

Hazim O. Khalifa ◽

Ahmed M. Soliman ◽

Ashraf M. Ahmed ◽

Alaaddin M. Hussein ◽

...

Keyword(s):

Escherichia Coli ◽

Colistin Resistance ◽

Coli Isolate

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Emergence of colistin resistance genes (mcr-1) in Escherichia coli among widely distributed wild ungulates

Environmental Pollution ◽

10.1016/j.envpol.2021.118136 ◽

2021 ◽

pp. 118136

Author(s):

Rita Tinoco Torres ◽

Mónica V. Cunha ◽

Débora Araujo ◽

Helena Ferreira ◽

Carlos Fonseca ◽

...

Keyword(s):

Escherichia Coli ◽

Resistance Genes ◽

Wild Ungulates ◽

Colistin Resistance

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Molecular survey of mcr1 and mcr2 plasmid mediated colistin resistance genes in Escherichia coli isolates of animal origin in Iran

BMC Research Notes ◽

10.1186/s13104-021-05519-6 ◽

2021 ◽

Vol 14 (1) ◽

Author(s):

Kayhan Ilbeigi ◽

Mahdi Askari Badouei ◽

Hossein Vaezi ◽

Hassan Zaheri ◽

Sina Aghasharif ◽

...

Keyword(s):

Escherichia Coli ◽

Resistance Genes ◽

Companion Animals ◽

Multidrug Resistant ◽

Animal Origin ◽

Colistin Resistance ◽

E Coli ◽

High Level ◽

Farming Industry

Abstract Objectives The emergence of colistin-resistant Enterobacteriaceae from human and animal sources is one of the major public health concerns as colistin is the last-resort antibiotic for treating infections caused by multidrug-resistant Gram-negative bacteria. We aimed to determine the prevalence of the prototype widespread colistin resistance genes (mcr-1 and mcr-2) among commensal and pathogenic Escherichia coli strains isolated from food-producing and companion animals in Iran. Results A total of 607 E. coli isolates which were previously collected from different animal sources between 2008 and 2016 used to uncover the possible presence of plasmid-mediated colistin resistance genes (mcr-1 and mcr-2) by PCR. Overall, our results could not confirm the presence of any mcr-1 or mcr-2 positive E. coli among the studied isolates. It is concluded that despite the important role of food-producing animals in transferring the antibiotic resistance, they were not the main source for carriage of mcr-1 and mcr-2 in Iran until 2016. This study suggests that the other mcr variants (mcr-3 to mcr-9) might be responsible for conferring colistin resistance in animal isolates in Iran. The possible linkage between pig farming industry and high level of mcr carriage in some countries needs to be clarified in future prospective studies.

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