scholarly journals Changes in nitric oxide synthase and nitrite and nitrate serum levels in patients with or without MDR-TB undergoing the intensive phase of anti-tuberculosis therapy

2014 ◽  
Vol 3 (2) ◽  
pp. 139-143 ◽  
Author(s):  
Dmytro О. Butov ◽  
Mikhail M. Kuzhko ◽  
Irina М. Kalmykovа ◽  
Irina М. Kuznetsova ◽  
Tatyana S. Butova ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Serum Levels ◽  
Intensive Phase ◽  
Mdr Tb ◽  
Tuberculosis Therapy ◽  
Oxide Synthase

Triiodothyronine attenuates the progression of renal injury in a rat model of chronic kidney disease

2018 ◽  
Vol 96 (6) ◽  
pp. 603-610 ◽  
Author(s):  
Sahar M. El Agaty
Keyword(s):  
Nitric Oxide ◽  
Chronic Kidney Disease ◽  
Nitric Oxide Synthase ◽  
Kidney Disease ◽  
Renal Injury ◽  
Serum Levels ◽  
Female Rats ◽  
Renal Tubules ◽  
Oxide Synthase ◽  
Remnant Kidney

This study was designed to investigate whether and how triiodothyronine (T3) affects renal function in an experimental model of chronic kidney disease. Twenty-four female rats were divided into the following groups: sham-operated control group (n = 8), 5/6 nephrectomized group (Nx, n = 8), and 5/6 nephrectomized group treated with T3 for 2 weeks (T3-Nx, n = 8). T3 administration significantly decreased serum levels of urea, creatinine, tumour necrosis factorα, and interleukin-6 compared with serum levels in the Nx group. The levels of malondialdehyde, transforming growth factor β, fibronectin, and collagen IV, as well as the expression of inducible nitric oxide synthase, nuclear factor κB, poly(ADP-ribose) polymerase, caspase-3, and Bax were all significantly decreased, though not normalized, in the remnant kidney of rats in the T3-Nx group compared with Nx rats. Glutathione, heme oxygenase-1 levels, as well as endothelial nitric oxide synthase expression were increased in the remnant kidney of the T3-Nx group. Histological studies revealed focal necrosis of renal tubules associated with inflammatory cell infiltration and fibrosis in the Nx group. These changes were alleviated in T3-Nx rats. This study showed that T3 administration attenuated the clinical and histological signs of renal injury in 5/6 nephrectomized rats by mitigating renal oxidative stress, inflammation, apoptosis, and fibrosis.

Pulmonary vascular dilation induced by l‐arginine: correlation with induction of endothelial nitric oxide synthase in a rabbit model

2005 ◽  
Vol 46 (1) ◽  
pp. 48-54 ◽  
Author(s):  
S. K. Yoon ◽  
K.‐N. Lee ◽  
J. H. Lee ◽  
J. S. Jeong ◽  
J.‐Y. Kwak
Keyword(s):  
Nitric Oxide ◽  
Computed Tomography ◽  
Nitric Oxide Synthase ◽  
Endothelial Nitric Oxide Synthase ◽  
Tap Water ◽  
Rabbit Model ◽  
Serum Levels ◽  
Control Group ◽  
Oxide Synthase ◽  
Endothelial Nitric Oxide

Purpose: To determine whether oral administration of l‐arginine induces pulmonary vascular dilation, and if this pulmonary vascular dilation correlates with induction of endothelial nitric oxide synthase (eNOS) in a rabbit model. Material and Methods: Seven rabbits were fed with l‐arginine dissolved in tap water. The degree of pulmonary vascular dilation was determined using thin‐section computed tomography and the concentration of serum nitrite was measured. They were compared with four control animals. The pulmonary vascular dilation was correlated to serum levels of nitrite. Lung tissues were examined for induction of eNOS by immunohistochemistry. Results: An increased degree of pulmonary vascular dilation was found in the l‐arginine‐fed group compared to the control group ( P<0.05). Serum levels of nitrite in the l‐arginine‐fed group were higher than those in the control group ( P<0.05). Pulmonary vascular dilation correlated with serum levels of nitrite ( r2 = 0.95, P<0.05). Induction of eNOS was increased in the l‐arginine‐fed group. Conclusion: The administration of l‐arginine causes pulmonary vascular dilation, which is most likely mediated via nitric oxide through increased induction of eNOS in a rabbit model.

Serum levels of cathepsin D, sirtuin1, and endothelial nitric oxide synthase are correlatively reduced in elderly healthy people

2015 ◽  
Vol 28 (4) ◽  
pp. 641-645 ◽  
Author(s):  
Yuan Zhong ◽  
Alex. F. Chen ◽  
Jian Zhao ◽  
Ying-Jia Gu ◽  
Guo-Xiang Fu
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Endothelial Nitric Oxide Synthase ◽  
Cathepsin D ◽  
Serum Levels ◽  
Healthy People ◽  
Oxide Synthase ◽  
Endothelial Nitric Oxide

Inducible nitric oxide synthase (iNOS) mediates ethanol-induced redox imbalance and up-regulation of inflammatory cytokines in the kidney

2021 ◽  
Author(s):  
Carla Brigagão Pacheco da Silva ◽  
Carla Speroni Ceron ◽  
Atlante Mendes ◽  
Bruno De Martinis ◽  
Michele Mazzaron de Castro ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Inflammatory Cytokines ◽  
Thiobarbituric Acid ◽  
Serum Levels ◽  
Ethanol Consumption ◽  
Tnf Α ◽  
Oxide Synthase ◽  
Deficient Mice

Overexpression of the inducible isoform of the enzyme nitric oxide synthase (iNOS) has been associated to pathological processes in the kidney. Ethanol consumption induces the renal expression of iNOS. However, the contribution of this enzyme to the deleterious effects of ethanol in the kidney remains elusive. We examined whether iNOS plays a role in the renal dysfunction and oxidative stress induced by ethanol consumption. With this purpose, male C57BL/6 wild-type (WT) or iNOS-deficient (iNOS-/-) mice were treated with ethanol (20% v/v) for 10 weeks. Treatment with ethanol increased the expression of Nox4 as well as the concentration of thiobarbituric acid reactive substances (TBARS) and the levels of tumor necrosis factor (TNF)-α in the renal cortex of WT, but not iNOS-deficient mice. Augmented serum levels of creatinine and increased systolic blood pressure were found in WT and iNOS-deficient mice treated with ethanol. WT mice treated with ethanol showed increased production of reactive oxygen species (ROS) and myeloperoxidase (MPO) activity, but these responses were attenuated in iNOS-deficient mice. We concluded that iNOS played a role in ethanol-induced oxidative stress and pro-inflammatory cytokines production in the kidney. These are mechanisms that may contribute to the renal toxicity induced by ethanol.

scholarly journals White Peony (Fermented Camellia sinensis) Polyphenols Help Prevent Alcoholic Liver Injury via Antioxidation

Antioxidants ◽  
2019 ◽  
Vol 8 (11) ◽  
pp. 524 ◽  
Author(s):  
Yalin Zhou ◽  
Fang Tan ◽  
Chong Li ◽  
Wenfeng Li ◽  
Wei Liao ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Superoxide Dismutase ◽  
Nitric Oxide Synthase ◽  
Liver Injury ◽  
Camellia Sinensis ◽  
Quantitative Polymerase Chain Reaction ◽  
Radical Scavenging ◽  
Serum Levels ◽  
Alcoholic Liver Injury ◽  
Oxide Synthase

White peony is a type of white tea (Camellia sinensis) rich in polyphenols. In this study, polyphenols were extracted from white peony. In vitro experiments showed that white peony polyphenols (WPPs) possess strong free radical scavenging capabilities toward 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS). Long-term alcohol gavage was used to induce alcoholic liver injury in mice, and relevant indices of liver injury were examined. WPPs effectively reduced the liver indices of mice with liver injury. The serum levels of aspartate aminotransferase (ATS), alanine aminotransferase (ALT), alkaline phosphatase (ALP), triglycerides (TG), total cholesterol (TC), blood urea nitrogen (BUN), nitric oxide (NO), and malondialdehyde (MDA) were downregulated, while those of albumin (ALB), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) were upregulated. WPPs also reduced the serum levels of interluekin-6 (IL-6), interluekin-12 (IL-12), tumor necrosis factor-alpha (TNF-α), and interferon-gamma (IFN-γ) in mice with liver injury. Pathology results showed that WPPs reduced alcohol-induced liver cell damage. Quantitative polymerase chain reaction (qPCR) and western blot results revealed that WPPs upregulated the mRNA and protein expressions of neuronal nitric oxide synthase (nNOS), endothelial nitric oxide synthase (eNOS), manganese superoxide dismutase (Mn-SOD), cupro–zinc superoxide dismutase (Cu/Zn-SOD), and CAT and downregulated iNOS expression in the liver of mice with liver injury. WPPs protected against alcoholic liver injury, and this effect was equivalent to that of silymarin. High-performance liquid chromatography revealed that WPPs mainly contained the polyphenols gallic acid, catechinic acid, and hyperoside, which are critical for exerting preventive effects against alcoholic liver injury. Thus, WPPs are high-quality natural products with liver protective effects.

scholarly journals Wu Shan Shen Cha (Malus asiatica Nakai. Leaves)-Derived Flavonoids Alleviate Alcohol-Induced Gastric Injury in Mice via an Anti-Oxidative Mechanism

Biomolecules ◽  
2019 ◽  
Vol 9 (5) ◽  
pp. 169 ◽  
Author(s):  
Bihui Liu ◽  
Chengfeng Zhang ◽  
Jing Zhang ◽  
Xin Zhao
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Hydrochloric Acid ◽  
Quantitative Polymerase Chain Reaction ◽  
Gastric Wall ◽  
Serum Levels ◽  
Gastric Injury ◽  
High Concentration ◽  
Tissue Specimens ◽  
Oxide Synthase

Wu Shan Shen Cha is the leaf of Malus asiatica Nakai., a special type of tea that is consumed in the same way as green tea. To study the effect of Wu Shan Shen Cha-derived flavonoids (WSSCF) on lesions in the stomach, a 15% hydrochloric acid–95% ethanol (volume ratio 4:6) solution was used to induce gastric injury in mice. The degree of gastric injury was assessed using tissue specimens, and the effects of WSSCF on the serum levels of antioxidant enzymes were investigated. The results showed that WSSCF could alleviate the damage of the gastric mucosa and gastric wall caused by the hydrochloric acid–ethanol solution, decrease the tissue and serum levels of malondialdehyde (MDA) in mice with gastric injury, and increase the serum levels of superoxide dismutase (SOD) and glutathione (GSH). The results of quantitative polymerase chain reaction (qPCR) showed that WSSCF could increase the mRNA expression of Mn-SOD, Cu/Zn-SOD, catalase (CAT), endothelial nitric oxide synthase (eNOS), and neuronal nitric oxide synthase (nNOS) in tissue specimens from mice with gastric injury and decrease the expression of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS). At the same time, the results of the high concentration of WSSCF (WSSCFH) group were closer to those of the drug (ranitidine) treatment group. Wu Shan Shen Cha-derived flavonoids had a good antioxidant effect, so as to play a preventive role in alcoholic gastric injury.

scholarly journals Serum levels of total antioxidant status, nitric oxide and nitric oxide synthase in minor recurrent aphthous stomatitis patients

Medicine ◽  
2019 ◽  
Vol 98 (3) ◽  
pp. e14039 ◽  
Author(s):  
Zichuan Zhang ◽  
Qian Zhang ◽  
Yi Xue ◽  
Guang Chen ◽  
Zhongyin Wu ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Antioxidant Status ◽  
Total Antioxidant Status ◽  
Serum Levels ◽  
Recurrent Aphthous Stomatitis ◽  
Aphthous Stomatitis ◽  
Total Antioxidant ◽  
Oxide Synthase
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