Acetone–butanol–ethanol production by separate hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF) methods using acorns and wood chips of Quercus acutissima as a carbon source

2014 ◽  
Vol 62 ◽  
pp. 286-292 ◽  
Author(s):  
Chizuru Sasaki ◽  
Yohei Kushiki ◽  
Chikako Asada ◽  
Yoshitoshi Nakamura
Keyword(s):  
Carbon Source ◽  
Ethanol Production ◽  
Simultaneous Saccharification And Fermentation ◽  
Wood Chips ◽  
Quercus Acutissima ◽  
Separate Hydrolysis And Fermentation ◽  
Acetone Butanol Ethanol ◽  
Simultaneous Saccharification

Ethanol Production from Hydrothermal Pretreated Empty Fruit Bunches

2014 ◽  
Vol 917 ◽  
pp. 80-86
Author(s):  
Mohd Saman Siti Aisyah ◽  
Pacharakamol Petchpradab ◽  
Yoshimitsu Uemura ◽  
Suzana Yusup ◽  
Machi Kanna ◽  
...  
Keyword(s):  
Ethanol Production ◽  
Lignocellulosic Biomass ◽  
Simultaneous Saccharification And Fermentation ◽  
Process Time ◽  
Empty Fruit Bunches ◽  
Separate Hydrolysis And Fermentation ◽  
Common Process ◽  
The Common ◽  
Ssf Process ◽  
Simultaneous Saccharification

Separate hydrolysis and fermentation (SHF) is the common process in producing ethanol from lignocellulosic biomass. Nowadays, simultaneous saccharification and fermentation (SSF) process has been seen as potential process for producing ethanol with shortens process time with higher yield of ethanol. Hence, in the current work, the utilization of empty fruit bunches (EFB) in SSF process was studied. In order to improve saccharification reactivity of EFB, hydrothermal pretreatment at 180 and 220 °C was used to pretreat EFB. The findings showed that SSF has the potential in producing ethanol from EFB.

scholarly journals Different schemas of saccharification and fermentation for bioethanol production from Opuntia ficus-indica cladode flour with wild strains

Bionatura ◽  
2018 ◽  
Vol 01 (Bionatura Conference Serie) ◽  
Author(s):  
Cindy Mariel López-Domínguez ◽  
Manuel Octavio Ramírez-Sucre ◽  
Ingrid Mayanín Rodríguez-Buenfil
Keyword(s):  
Carbon Source ◽  
Kluyveromyces Marxianus ◽  
Simultaneous Saccharification And Fermentation ◽  
Alcohol Concentration ◽  
Opuntia Ficus Indica ◽  
Alcohol Production ◽  
Separate Hydrolysis And Fermentation ◽  
Significant Difference ◽  
Acinetobacter Pittii ◽  
Simultaneous Saccharification

In the present work, two wild microorganisms were studied for saccharification and fermentation. A wild Acinetobacter pittii isolated from decaying cladodes (Opuntia ficus-indica) was capable of producing extracellular cellulases and a wild yeast Kluyveromyces marxianus isolated from termite was capable of producing alcohol. In Mexico, there are surpluses of cladode production and where it is essential to take advantage and use this carbon source for alcohol production due to currently fossil fuels depletion. Separate hydrolysis and fermentation (SHF), simultaneous saccharification and fermentation (SSF) and semi-simultaneous saccharification and fermentation (SSSF) for cellulase and alcohol production using Opuntia ficus-indica cladode as a unique carbon source was evaluated. In SHF process the best conditions for FPase activity (Filter paper activity for total cellulases) were 37 °C and pH 6.5 obtaining 0.67±0.02 U/ml and 0.61±0.03 U/ml for Acinetobacter pittii and Kluyveromyces marxianus, respectively. For alcohol production, the best conditions were 40 °C and pH 5.5 obtaining 12.95±0.3 g/L with K. marxianus while A. pittii did not produce significant alcohol concentration. Both processes were made with agitation (200 rpm). The SSF process was made with both microorganisms inoculated at the same time at 37 °C and without agitation. The maximum FPase activity of 0.28±0.004 U/ml and the maximum alcohol concentration was 7.5±0.27 g/L. Finally, an SSSF was performed, initially with A. pittii at 37 °C and after 8 h K. marxianus was then inoculated with temperature switched to 40 °C, the all process was performed without agitation. The maximum FPase activity was 0.45±0.001 U/ml, and the maximum alcohol concentration was 11.7±0.02 g/L. There was a significant difference (ANOVA) between SHF and SSSF in alcohol production. The best process for FPase activity and alcohol production is separate hydrolysis and fermentation using only yeast Kluyveromyces marxianus.

Ethanol Production from Corn Stover Using Soaking Pretreatment

2010 ◽  
Vol 171-172 ◽  
pp. 261-265
Author(s):  
Zhuang Zuo ◽  
Xiu Shan Yang
Keyword(s):  
Ethanol Production ◽  
Corn Stover ◽  
Simultaneous Saccharification And Fermentation ◽  
Pichia Stipitis ◽  
Liquid Ratio ◽  
Mild Conditions ◽  
Whole Process ◽  
Conversion Rates ◽  
Mild Temperature ◽  
Simultaneous Saccharification

Corn stover was pretreated using different soaking conditions at mild temperature. Among the tested conditions, the best was 1% NaOH+8% NH4OH，50°C，48 h, Solid-to-liquid ratio 1:10. The results showed that soaking pretreatment achieved 63.6% delignification, retained the xylan and glucan. After enzymatic hydrolysis, conversion rates of xylan and glucan were 70.9% and 78.5%, respectively. The pretreated filtration re-soaking cause 52.7% xylan and 65.0% glucan conversion. NaOH+NH4OH treatment can be performed under mild conditions, gives a good buffering effect, low carbohydates degradation and extensive removal of lignin. Additionally, simultaneous saccharification and fermentation was conducted with pretreated corn stover to assess the ethanol production. For the whole process, 0.15g ethanol /g corn stover was achieved using Saccharomyces cerevisiae Y5, and 0.19g ethanol /g corn stover when using Pichia stipitis.

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