Antioxidant capacity and phenolic content of commonly used anti-inflammatory medicinal plants in Colombia

2015 ◽  
Vol 70 ◽  
pp. 272-279 ◽  
Author(s):  
Nora Jiménez ◽  
Luis Carrillo-Hormaza ◽  
Andrea Pujol ◽  
Fernando Álzate ◽  
Edison Osorio ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Antioxidant Capacity ◽  
Phenolic Content ◽  
Anti Inflammatory

scholarly journals Evaluation of Antioxidant, Anti-Inflammatory and Cytoprotective Properties of Ethanolic Mint Extracts from Algeria on 7-Ketocholesterol-Treated Murine RAW 264.7 Macrophages

Antioxidants ◽  
2018 ◽  
Vol 7 (12) ◽  
pp. 184 ◽  
Author(s):  
Fatiha Brahmi ◽  
Thomas Nury ◽  
Meryam Debbabi ◽  
Samia Hadj-Ahmed ◽  
Amira Zarrouk ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Cytokine Secretion ◽  
Total Phenolic ◽  
Raw 264.7 ◽  
Dependent Manner ◽  
Raw 264.7 Macrophages ◽  
Tnf Α ◽  
Anti Inflammatory

The present study consisted in evaluating the antioxidant, anti-inflammatory and cytoprotective properties of ethanolic extracts from three mint species (Mentha spicata L. (MS), Mentha pulegium L. (MP) and Mentha rotundifolia (L.) Huds (MR)) with biochemical methods on murine RAW 264.7 macrophages (a transformed macrophage cell line isolated from ascites of BALB/c mice infected by the Abelson leukemia virus). The total phenolic, flavonoid and carotenoid contents were determined with spectrophotometric methods. The antioxidant activities were quantified with the Kit Radicaux Libres (KRLTM), the ferric reducing antioxidant power (FRAP) and the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assays. The MS extract showed the highest total phenolic content, and the highest antioxidant capacity, while the MR extract showed the lowest total phenolic content and the lowest antioxidant capacity. The cytoprotective and anti-inflammatory activities of the extracts were quantified on murine RAW 264.7 macrophages treated with 7-ketocholesterol (7KC; 20 µg/mL: 50 µM) associated or not for 24 h and 48 h with ethanolic mint extracts used at different concentrations (25, 50, 100, 200 and 400 µg/mL). Under treatment with 7KC, an important inhibition of cell growth was revealed with the crystal violet test. This side effect was strongly attenuated in a dose dependent manner with the different ethanolic mint extracts, mainly at 48 h. The most important cytoprotective effect was observed with the MS extract. In addition, the effects of ethanolic mint extracts on cytokine secretion (Interleukin (IL)-6, IL-10, Monocyte Chemoattractant Protein (MCP)-1, Interferon (IFN)-ϒ, Tumor necrosis factor (TNF)-α) were determined at 24 h on lipopolysaccharide (LPS, 0.2 µg/mL)-, 7KC (20 µg/mL)- and (7KC + LPS)-treated RAW 264.7 cells. Complex effects of mint extracts were observed on cytokine secretion. However, comparatively to LPS-treated cells, all the extracts strongly reduce IL-6 secretion and two of them (MP and MR) also decrease MCP-1 and TNF-α secretion. However, no anti-inflammatory effects were observed on 7KC- and (7KC + LPS)-treated cells. Altogether, these data bring new evidences on the potential benefits (especially antioxidant and cytoprotective properties) of Algerian mint on human health.

scholarly journals LC-ESI-QTOF/MS Characterization of Phenolic Compounds from Medicinal Plants (Hops and Juniper Berries) and Their Antioxidant Activity

Foods ◽  
2019 ◽  
Vol 9 (1) ◽  
pp. 7 ◽  
Author(s):  
Jiafei Tang ◽  
Frank R. Dunshea ◽  
Hafiz A. R. Suleria
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Medicinal Plants ◽  
Antioxidant Capacity ◽  
Phenolic Acids ◽  
Phenolic Content ◽  
Total Phenolic ◽  
High Concentration ◽  
Antioxidant Power ◽  
Pharmaceutical Industries

Hops (Humulus lupulus L.) and juniper berries (Juniperus communis L.) are two important medicinal plants widely used in the food, beverage, and pharmaceutical industries due to their strong antioxidant capacity, which is attributed to the presence of polyphenols. The present study is conducted to comprehensively characterize polyphenols from hops and juniper berries using liquid chromatography coupled with electrospray-ionization quadrupole time-of-flight mass spectrometry (LC-ESI-QTOF/MS) to assess their antioxidant capacity. For polyphenol estimation, total phenolic content, flavonoids and tannins were measured, while for antioxidant capacity, three different antioxidant assays including the 2,2-diphenyl-1-picrylhydrazyl (DPPH) antioxidant assay, the 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical cation decolorization assay and the ferric reducing-antioxidant power (FRAP) assay were used. Hops presented the higher phenolic content (23.11 ± 0.03 mg/g dw) which corresponded to its strong antioxidant activity as compared to the juniper berries. Using LC-ESI-QTOF/MS, a total of 148 phenolic compounds were tentatively identified in juniper and hops, among which phenolic acids (including hydroxybenzoic acids, hydroxycinnamic acids and hydroxyphenylpropanoic acids) and flavonoids (mainly anthocyanins, flavones, flavonols, and isoflavonoids) were the main polyphenols, which may contribute to their antioxidant capacity. Furthermore, the HPLC quantitative analysis showed that both samples had a high concentration of phenolic acids and flavonoids. In the HPLC quantification, the predominant phenolic acids in hops and juniper berries were chlorogenic acid (16.48 ± 0.03 mg/g dw) and protocatechuic acid (11.46 ± 0.03 mg/g dw), respectively. The obtained results highlight the importance of hops and juniper berries as a rich source of functional ingredients in different food, beverage, and pharmaceutical industries.

scholarly journals Antioxidant, Total Phenolic and Flavonoid Content of Selected Nigerian Medicinal Plants

2015 ◽  
Vol 14 (1) ◽  
pp. 35-41 ◽  
Author(s):  
Matthias Onyebuchi Agbo ◽  
Philip Felix Uzor ◽  
Uchenna Nneamaka Akazie Nneji ◽  
Chidozie Uzoma Eze Odurukwe ◽  
Uchenna Basilia Ogbatue ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Phenolic Content ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Total Antioxidant ◽  
Phenolic And Flavonoid

Plant phenolics and flavonoids play a great role in scavenging free radicals in the body and act as antioxidants. Thus their determination is sometimes nedded. Total antioxidant capacity, total phenolic and flavonoid contents of the extracts of 10 medicinal plants (Lochnera rosea, Allamanda cathartica, Asplenium platyneuron, Euphorbia prostrate, Baphia nitida, Crotolaria retusa, Zapoteca portoricensis, Platycerium bifurcatum, Mussaenda afzelii and Craterosiphon scandens) from 7 botanical families growing in the tropical rainforest of Nigeria were included in this study. The total antioxidant capacity of the extracts was assessed by using the phosphomolybdate method. The phenolic content was determined by using Folin-Ciocalteau assay, while the total flavonoid was determined by the aluminium chloride colorimetric assay. The results obtained showed that the total antioxidant capacity for all the extracts were in the range of 0.888 ± 0.75 to 0.938 ± 0.00 mg EAA/g. The results showed that E. prostrate, P. bifurcatum and A. platyneuron were found to be the richest source of phenolic (97.77 ± 0.77, 87.62 ± 1.22 and 82.33 ± 0.30 mg GAE/g) while B. nitida and M. afzelii had the least total phenolic content (11.67 ± 0.09 and11.18 ± 0.30 mg GAE/g). The highest total flavonoid content was revealed in P. bifurcatum (648.67 ± 12.3 mg QE) while M. afzelii also had the least total flavonoid content (3.67 ± 0.00 mg QE/g). The ratio of flavonoid to the phenolic in each extract was also determined to ascertain extracts that are rich in flavonoids.Dhaka Univ. J. Pharm. Sci. 14(1): 35-41, 2015 (June)

scholarly journals Antioxidant and Anti-Inflammatory Activities of Kenyan Leafy Green Vegetables, Wild Fruits, and Medicinal Plants with Potential Relevance for Kwashiorkor

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
H. R. Tufts ◽  
C. S. Harris ◽  
Z. N. Bukania ◽  
T. Johns
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plants ◽  
Medicinal Plant ◽  
Phenolic Content ◽  
Mangifera Indica ◽  
Acute Malnutrition ◽  
Total Phenolic ◽  
Wild Fruits ◽  
Prevention And Treatment ◽  
Anti Inflammatory

Background.Inflammation, together with related oxidative stress, is linked with the etiology of kwashiorkor, a form of severe acute malnutrition in children. A diet rich in anti-inflammatory and antioxidant phytochemicals may offer potential for the prevention and treatment of kwashiorkor. We selected and assayed five leafy green vegetables, two wild fruits, and six medicinal plants from Kenya for their antioxidant and anti-inflammatory properties. Consensus regarding medicinal plant use was established from ethnobotanical data.Methods. Antioxidant activity and phenolic content were determined using the oxygen radical absorbance capacity (ORAC) assay and Folin-Ciocalteu procedure, respectively. Anti-inflammatory activity was assessedin vitrotargeting the inflammatory mediator tumour necrosis factor-alpha (TNF-α).Results. Mangifera indica(leaves used medicinally) showed the greatest antioxidant activity (5940 ± 632 µM TE/µg) and total phenolic content (337 ± 3 mg GAE/g) butAmaranthus dubius(leafy vegetable) showed the greatest inhibition of TNF-α(IC50= 9 ± 1 μg/mL), followed byOcimum americanum(medicinal plant) (IC50= 16 ± 1 μg/mL). Informant consensus was significantly correlated with anti-inflammatory effects among active medicinal plants (r2=0.7639, P=0.0228).Conclusions. Several plant species commonly consumed by Kenyan children possess activity profiles relevant to the prevention and treatment of kwashiorkor and warrant further investigation.

scholarly journals Effects of Extraction Techniques on Total Phenolic Content and Antioxidant Capacities of Two Oregano Leaves

2014 ◽  
Vol 4 (1) ◽  
pp. 112 ◽  
Author(s):  
Ahmad Karimi ◽  
Byungjick Min ◽  
Cindi Brownmiller ◽  
Sun-Ok Lee
Keyword(s):  
Medicinal Plants ◽  
Antioxidant Capacity ◽  
Phenolic Content ◽  
Antioxidant Properties ◽  
Strong Relationship ◽  
Total Phenolic ◽  
Distilled Water ◽  
Extraction Techniques ◽  
Phenolic Contents ◽  
Solvent Type

<p>The effects of dried oregano leaves (Mediterranean and Mexican oregano) extracted using different extraction techniques, solvent types, and six different ratios of each solvent to distilled water on total phenolic (TP) content and antioxidant properties were examined. The Folin-Ciocalteu and 1,1-Diphenyl-2-picryl-hydrazyl (DPPH) assays were performed to assess the antioxidant capacity. The different species of oregano had a significant effect on TP content (107.6 vs. 34.5 mg GAEg<sup>-1</sup> in Mexican vs. Mediterranean oregano, respectively) (<em>P</em>&lt;0.05). Comparing extraction techniques, the vortex procedure significantly increased the measured TP content compared to sonication or shaking (<em>P</em>&lt;0.05); however, its effectiveness was sample species and solvent type dependent. Solvent type also had a significant impact on TP content of extracts in decreasing order of acetone, methanol, ethanol, and water (<em>P</em>&lt;0.05). The solvent:water ratio on TP content of each extract was significant (<em>P</em>&lt;0.05); higher TP content was measured for 40:60 and 60:40 acetone:water ratios for Mediterranean and 60:40 and 80:20 acetone:water ratios for Mexican oregano. The antioxidant capacity had a strong relationship with total phenolic contents. The current findings indicated that the species, extraction techniques, solvent type and the ratio of solvent:water had a significance influence on the TP content of two different species of dried oregano leaf, which may be a possible reason behind most variability reported on TP compounds of herbal and medicinal plants.</p>

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