Improved method to obtain essential oil, asarinin and sesamin from Asarum heterotropoides var. mandshuricum using microwave-assisted steam distillation followed by solvent extraction and antifungal activity of essential oil against Fusarium spp

2021 ◽  
Vol 162 ◽  
pp. 113295
Author(s):  
Yao Xiao ◽  
Zaizhi Liu ◽  
Huiyan Gu ◽  
Fengjian Yang ◽  
Lin Zhang ◽  
...  
2016 ◽  
Vol 12 (1) ◽  
pp. 66-73
Author(s):  
Daniel Plazas-Jiménez ◽  
Victor Enrique Macias-Villamizar ◽  
Ericsson Coy-Barrera

“Yellow moena” (Nectandra longifolia: Lauraceae) is a fragrant leaves-possessing plant commonly used for timber purposes. Nevertheless, so far, there is not available information about its chemical composition. Thus, in the present work is described the components of the essential oil from N. longifolia leaves. The oil was extracted by steam distillation and analyzed by GC/FID  and GC/MS. Twenty eight compounds were therefore identified. The main components were found to be terpinen-4-ol (53.1%), α-terpinene (6.8%), α-phellandrene (4.5%), and β-pinene (3.8%). The chemical composition of essential oil from N. longifolia leaves was consequently in agreement to that of several neotropical Lauraceae plants. In addition, the oil exhibited good antifungal activity against F. oxysporum through dose-dependent mycelial growth inhibition (using amended media and fumigant assays).


2017 ◽  
Vol 1 (1) ◽  
pp. 33 ◽  
Author(s):  
Wiwik Susanah Rita ◽  
Retno Kawuri ◽  
I Made Dira Swantara

Acorus calamus L. rhizome was trusted having antibacterial activity. This study aimed to identify the compounds in the Acorus Calamus L. rhizomes essential oils and to recognize the antifungal activity of the oils against Candida albicans. The extraction of essential oils from rhizome was carried out by steam distillation technique. Identification of compounds in the oils was conducted by Gas Chromatography- Mass Spectroscopy (GCMS), while the antifungal test against Candida albicans was done by well diffusion method. Extraction of 10 kg of rhizomes produced 16.53 mL essential oil with a yield of 0.1653% (? = 1.066), the oil was brownish yellow and very flavorful. GC-MS analysis showed that the essential oil contained 11 compounds, they are (E)-3,7 dimethyl-1,3,6-Octatriene (trans-?-Ocimene) (3,73%), linalool (1,07%), ?-elemene (1,15%), trans methyl isoeugenol (7,68%), shyobunon (15,74%), bicyclogermakren (0,93%), dehidroxy-isocalamendiol (2,61%), ?-calacorene (3,34%), euasarone (26,84), cis-asarone (18,62%); dan trans- asarone (18,29%). Antifungal activity test showed that the growth and biomass inhibition of C. albicans increased with the increase of the oil concentration. Minimum Inhibitory Concentration (MIC) of essential oil toward C. albicans was 1% with the inhibition of 7.83 mm.


Agronomy ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1098
Author(s):  
César Chacón ◽  
Emanuel Bojórquez-Quintal ◽  
Goretty Caamal-Chan ◽  
Víctor M. Ruíz-Valdiviezo ◽  
Joaquín A. Montes-Molina ◽  
...  

The essential oils of plants of the genus Piper have secondary metabolites that have antimicrobial activity related to their chemical composition. The objective of our work was to determine the chemical composition and evaluate the antifungal activity of the aerial part essential oil of P. auritum obtained by hydrodistillation on Fusarium oxysporum and Fusarium equiseti isolated from Capsicum chinense. The antifungal activity was evaluated by direct contact and poisoned food tests, and the minimum inhibitory concentration (MIC50) and maximum radial growth inhibition (MGI) were determined. The identification of oil metabolites was carried out by direct analysis in real time mass spectrometry (DART-MS). By direct contact, the essential oil reached an inhibition of over 40% on Fusarium spp. The 8.4 mg/mL concentration showed the highest inhibition on F. oxysporum (40–60%) and F. equiseti (>50%). The MIC50 was 6 mg/mL for F. oxysporum FCHA-T7 and 9 mg/mL for F. oxysporum FCHJ-T6 and F. equiseti FCHE-T8. DART-MS chemical analysis of the essential oil showed [2M-H]− and [M-H]− adducts of high relative intensity that were mainly attributed to eugenol and thymol/p-cimen-8-ol. The findings found in this study show a fungistatic effect of the essential oil of P. auritum on Fusarium spp.


2006 ◽  
Vol 3 (1) ◽  
pp. 31 ◽  
Author(s):  
A.A. Saoud ◽  
R.M. Yunus ◽  
R.A. Aziz

Classical extraction of essential oil such as Soxhlet and steam distillation is still a formidable and time-solvent consuming. Microwave assisted process (MAP) is used to accelerate the extraction process of target compounds. It can be used for the extraction of compounds from various plants and animal tissues, or the extraction of undesirable components from raw materials. The investigation of microwave extraction of eucalyptus (globules ) essential oil using ethanol as solvent was carried out. The influence of material (eucalyptus)/solvent (ethanol) ratio, required doses of microwave, and time of microwave exposure on extraction efficiency, was studied. 


2006 ◽  
Vol 49 (6) ◽  
pp. 867-871 ◽  
Author(s):  
Terezinha de Jesus Faria ◽  
Rafael Sottero Ferreira ◽  
Lidiane Yassumoto ◽  
José Roberto Pinto de Souza ◽  
Noemia Kazue Ishikawa ◽  
...  

An investigation of antifungal activity of the essential oil obtained by steam-distillation (1.1% w/w) of the aerial parts of Ocimum gratissimum and of an ethanolic extract from the steam-distillation residue was carried out using the agar diffusion method. The results revealed that the essential oil inhibited the growth of all fungi tested, including the phytopathogens, Botryosphaeria rhodina, Rhizoctonia sp. and two strains of Alternaria sp., while the extract from the residue was inactive. The essential oil was subjected to TLC bioautography used to detect fungitoxic constituents. The compound that showed antifungal activity was isolated and identified as eugenol. GC/MS analysis showed that eugenol was the main constituent of the essential oil studied. The antifungal activity of eugenol was evaluated against a species of Alternaria isolated from tomato (A1) and Penicillium chrysogenum. The minimal inhibitory concentrations of eugenol were 0.16 and 0.31 mg/disc for Alternaria sp. (A1) and P. chrysogenum, respectively.


2011 ◽  
Vol 29 (1) ◽  
pp. 130-133 ◽  
Author(s):  
Yue-Lin ZHU ◽  
Zeng-Hua CHANG ◽  
Xiao-Mei ZHENG ◽  
Zhi-Ping HUANG ◽  
Kai-Wen XIAO

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