In-situ corn fiber conversion method unlocks the role of viscosity on enhancing ethanol yield by reducing side-product glycerol

2021 ◽  
Vol 169 ◽  
pp. 113653
Author(s):  
Xiujuan Li ◽  
Qiang Xiong ◽  
Minghui Wang ◽  
He Huang ◽  
Guojun Yue ◽  
...  
Keyword(s):  
Ethanol Yield ◽  
Corn Fiber ◽  
Side Product ◽  
Conversion Method

In Situ Corn Fiber Conversion for Ethanol Improvement by The Addition of Novel Lignocellulolytic Enzyme Cocktail

2020 ◽  
Author(s):  
Le Gao ◽  
Shulin Chen ◽  
Dongyuan Zhang
Keyword(s):  
Batch Fermentation ◽  
Ethanol Yield ◽  
Fermentation Broth ◽  
High Viscosity ◽  
Corn Fiber ◽  
Yeast Fermentation ◽  
Lignocellulolytic Enzymes ◽  
High Sugar ◽  
Corn Mash

Abstract Background: The technology of converting corn mashes to ethanol has been mature, but corn mashes has high-viscosity and high-sugar characteristics which hindered cellulose utilization and yeast-fermentation efficiency. The excessive viscosity of corn mash is caused by the presence of non-starch polysaccharides, such as cellulose in cereal grains. Corn kernel fiber (mostly cellulose) is typically unconverted in the process. Results: A novel lignocellulolytic enzymes cocktail with strong substrate specificity was prepared for high-viscosity, high-sugar corn mash. The in situ conversion of corn mashes with novel lignocellulolytic enzymes at the optimum cellulase dosage of 50 FPU/L resulted in 12.4%, 12.0%, 11.8%, and 12.9% increased ethanol concentration compared with the reference mash at 0.3, 1, 5, and 70 L batch-fermentation scales, respectively. The highest yield of ethanol from corn mash digested with the prepared novel lignocellulolytic enzyme reached 117.0 ± 0.1g/L at the 70 L batch fermentation, which was a 12.9% increase in ethanol yield. Adding the lignocellulolytic enzymes caused the greatest decrease in viscosity of corn mash by 40.9% compared with the reference mash (33.5 ± 1.5 Pa·s), whereas the residual sugars decreased by 56.3%. Simultaneously, the application of novel lignocellulolytic enzymes increased the value of dried distiller’s grain with solubles by increasing the protein content and decreasing the residual cellulose and starch content.Conclusion: The application of novel lignocellulolytic enzymes significantly improved the alcohol concentration, productivity, and yield. With the same amount of material, the application of the novel enzymes cocktail can enhance the ethanol yield by more than 10%. The in situ conversion of cellulose promoted the release of contents, including starch and protein, which can decrease the fermentation broth viscosity and improve the rheological property, thereby improving the ethanol yield. Thus, this technology can increase the net revenue of fuel-ethanol industrialization and promote the technological progress of renewable energy.

In situ pretreatment during distillation improves corn fiber conversion and ethanol yield in the dry mill process

2019 ◽  
Vol 21 (5) ◽  
pp. 1080-1090 ◽  
Author(s):  
Xiujuan Li ◽  
Zhaoxian Xu ◽  
Jianming Yu ◽  
He Huang ◽  
Mingjie Jin
Keyword(s):  
Ethanol Yield ◽  
Corn Fiber ◽  
Cellulose Conversion ◽  
Mill Process

The in situ pretreatment and in situ conversion of corn fiber increased cellulose conversion and the overall ethanol yield.

In-situ corn fiber conversion improves ethanol yield in corn dry-mill process

2018 ◽  
Vol 113 ◽  
pp. 217-224 ◽  
Author(s):  
Xiujuan Li ◽  
Sitong Chen ◽  
He Huang ◽  
Mingjie Jin
Keyword(s):  
Ethanol Yield ◽  
Corn Fiber ◽  
Mill Process

{10-12} Twinning Mechanism During In Situ Micro-Tensile Loading of Pure Mg: Role of Basal Slip and Twin-Twin Boundary Interaction

2020 ◽  
Author(s):  
Nicolò Maria della Ventura ◽  
Szilvia Kalácska ◽  
Daniele Casari ◽  
Thomas Edward James Edwards ◽  
Johann Michler ◽  
...  
Keyword(s):  
Twin Boundary ◽  
Basal Slip ◽  
Tensile Loading ◽  
Boundary Interaction

The role of (bio)surfactant sorption in promoting the bioavailability of nutrients localized at the solid-water interface

1999 ◽  
Vol 39 (7) ◽  
pp. 91-98 ◽  
Author(s):  
Ryan N. Jordan ◽  
Eric P. Nichols ◽  
Alfred B. Cunningham
Keyword(s):  
Mass Transfer ◽  
Cell Membrane ◽  
Substrate Concentration ◽  
Water Interface ◽  
Industrial Systems ◽  
Solid Liquid Interface ◽  
Solid Liquid ◽  
Surfactant Sorption

Bioavailability is herein defined as the accessibility of a substrate by a microorganism. Further, bioavailability is governed by (1) the substrate concentration that the cell membrane “sees,” (i.e., the “directly bioavailable” pool) as well as (2) the rate of mass transfer from potentially bioavailable (e.g., nonaqueous) phases to the directly bioavailable (e.g., aqueous) phase. Mechanisms by which sorbed (bio)surfactants influence these two processes are discussed. We propose the hypothesis that the sorption of (bio)surfactants at the solid-liquid interface is partially responsible for the increased bioavailability of surface-bound nutrients, and offer this as a basis for suggesting the development of engineered in-situ bioremediation technologies that take advantage of low (bio)surfactant concentrations. In addition, other industrial systems where bioavailability phenomena should be considered are addressed.

THE ROLE OF BACTERIAL COMMUNITIES ON IN-SITU ARSENIC AND IRON CYCLING IN RIVERBANKS ALONG THE MEGHNA RIVER, BANGLADESH

2020 ◽  
Author(s):  
Kimberly D. Myers ◽  
◽  
Katrina Lee Jewell ◽  
P.S.K. Knappett ◽  
Mehtaz M. Lipsi ◽  
...  
Keyword(s):  
Bacterial Communities ◽  
Iron Cycling

How Artefacts Do Leadership: A Ventriloquial Analysis

2021 ◽  
pp. 089331892199807
Author(s):  
Jonathan Clifton ◽  
Fernando Fachin ◽  
François Cooren
Keyword(s):  
Organizational Communication ◽  
Recent Work ◽  
Distributed Leadership ◽  
Network Theory ◽  
Actor Network Theory ◽  
Fine Grained ◽  
Naturally Occurring ◽  
Human Actors

To date there has been little work that uses fine-grained interactional analyses of the in situ doing of leadership to make visible the role of non-human as well as human actants in this process. Using transcripts of naturally-occurring interaction as data, this study seeks to show how leadership is co-achieved by artefacts as an in-situ accomplishment. To do this we situate this study within recent work on distributed leadership and argue that it is not only distributed across human actors, but also across networks that include both human and non-human actors. Taking a discursive approach to leadership, we draw on Actor Network Theory and adopt a ventriloquial approach to sociomateriality as inspired by the Montreal School of organizational communication. Findings indicate that artefacts “do” leadership when a hybrid presence is made relevant to the interaction and when this presence provides authoritative grounds for influencing others to achieve the group’s goals.

scholarly journals Phosphorylation of GAPVD1 Is Regulated by the PER Complex and Linked to GAPVD1 Degradation

2021 ◽  
Vol 22 (7) ◽  
pp. 3787
Author(s):  
Hussam Ibrahim ◽  
Philipp Reus ◽  
Anna Katharina Mundorf ◽  
Anna-Lena Grothoff ◽  
Valerie Rudenko ◽  
...  
Keyword(s):  
Circadian Clock ◽  
Transcriptional Repression ◽  
Small Gtpase ◽  
Main Role ◽  
Interacting Proteins ◽  
Casein Kinase 1 ◽  
Bona Fide ◽  
Kinetics Of

Repressor protein period (PER) complexes play a central role in the molecular oscillator mechanism of the mammalian circadian clock. While the main role of nuclear PER complexes is transcriptional repression, much less is known about the functions of cytoplasmic PER complexes. We found with a biochemical screen for PER2-interacting proteins that the small GTPase regulator GTPase-activating protein and VPS9 domain-containing protein 1 (GAPVD1), which has been identified previously as a component of cytoplasmic PER complexes in mice, is also a bona fide component of human PER complexes. We show that in situ GAPVD1 is closely associated with casein kinase 1 delta (CSNK1D), a kinase that regulates PER2 levels through a phosphoswitch mechanism, and that CSNK1D regulates the phosphorylation of GAPVD1. Moreover, phosphorylation determines the kinetics of GAPVD1 degradation and is controlled by PER2 and a C-terminal autoinhibitory domain in CSNK1D, indicating that the regulation of GAPVD1 phosphorylation is a novel function of cytoplasmic PER complexes and might be part of the oscillator mechanism or an output function of the circadian clock.

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