Electrical stimulation to human dermal papilla cells for hair regenerative medicine

2022 ◽  
Author(s):  
Lei Yan ◽  
Tatsuto Kageyama ◽  
Binbin Zhang ◽  
Seiya Yamashita ◽  
Paul J. Molino ◽  
...  
Keyword(s):  
Electrical Stimulation ◽  
Regenerative Medicine ◽  
Dermal Papilla ◽  
Dermal Papilla Cells

scholarly journals Hair follicle germs containing vascular endothelial cells for hair regenerative medicine

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Tatsuto Kageyama ◽  
Yang-Sook Chun ◽  
Junji Fukuda
Keyword(s):  
Endothelial Cells ◽  
Regenerative Medicine ◽  
Hair Follicle ◽  
Vascular Endothelial Cells ◽  
Morphological Characteristics ◽  
Dermal Papilla ◽  
Hair Follicles ◽  
Vascular Endothelial ◽  
Dermal Papilla Cells

AbstractHair regenerative medicine has emerged as a promising approach for the treatment of severe hair loss. Recent advances in three-dimensional tissue engineering, such as formation of hair follicle germs (HFGs), have considerably improved hair regeneration after transplantation in animal models. Here, we proposed an approach for fabricating HFGs containing vascular endothelial cells. Epithelial, dermal papilla, and vascular endothelial cells initially formed a single aggregate, which subsequently became a dumbbell-shaped HFG, wherein the vascular endothelial cells localized in the region of dermal papilla cells. The HFGs containing vascular endothelial cells exhibited higher expression of hair morphogenesis-related genes in vitro, along with higher levels of hair shaft regeneration upon transplantation to the dorsal side of nude mice, than those without vascular endothelial cells. The generated hair follicles represented functional characteristics, such as piloerection, as well as morphological characteristics comparable to those of natural hair shafts. This approach may provide a promising strategy for fabricating tissue grafts with higher hair inductivity for hair regenerative medicine.

scholarly journals Detecting the Mechanism behind the Transition from Fixed Two-Dimensional Patterned Sika Deer (Cervus nippon) Dermal Papilla Cells to Three-Dimensional Pattern

2021 ◽  
Vol 22 (9) ◽  
pp. 4715
Author(s):  
Guanning Wei ◽  
Hongmei Sun ◽  
Haijun Wei ◽  
Tao Qin ◽  
Yifeng Yang ◽  
...  
Keyword(s):  
Culture Condition ◽  
Sika Deer ◽  
Three Dimensional ◽  
Cervus Nippon ◽  
Dermal Papilla ◽  
Growth Patterns ◽  
The Self ◽  
Dermal Papilla Cells ◽  
Aggregative Behavior ◽  
Cell Pattern

The hair follicle dermal papilla is critical for hair generation and de novo regeneration. When cultured in vitro, dermal papilla cells from different species demonstrate two distinguishable growth patterns under the conventional culture condition: a self-aggregative three dimensional spheroidal (3D) cell pattern and a two dimensional (2D) monolayer cell pattern, correlating with different hair inducing properties. Whether the loss of self-aggregative behavior relates to species-specific differences or the improper culture condition remains unclear. Can the fixed 2D patterned dermal papilla cells recover the self-aggregative behavior and 3D pattern also remains undetected. Here, we successfully constructed the two growth patterns using sika deer (Cervus nippon) dermal papilla cells and proved it was the culture condition that determined the dermal papilla growth pattern. The two growth patterns could transit mutually as the culture condition was exchanged. The fixed 2D patterned sika deer dermal papilla cells could recover the self-aggregative behavior and transit back to 3D pattern, accompanied by the restoration of hair inducing capability when the culture condition was changed. In addition, the global gene expressions during the transition from 2D pattern to 3D pattern were compared to detect the potential regulating genes and pathways involved in the recovery of 3D pattern and hair inducing capability.

scholarly journals Modulation of Human Mesenchymal Stem Cells by Electrical Stimulation Using an Enzymatic Biofuel Cell

Catalysts ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 62
Author(s):  
Won-Yong Jeon ◽  
Seyoung Mun ◽  
Wei Beng Ng ◽  
Keunsoo Kang ◽  
Kyudong Han ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Electrical Stimulation ◽  
Regenerative Medicine ◽  
Cell Morphology ◽  
Electrical Current ◽  
Specific Gene ◽  
Next Generation ◽  
The Impact

Enzymatic biofuel cells (EBFCs) have excellent potential as components in bioelectronic devices, especially as active biointerfaces to regulate stem cell behavior for regenerative medicine applications. However, it remains unclear to what extent EBFC-generated electrical stimulation can regulate the functional behavior of human adipose-derived mesenchymal stem cells (hAD-MSCs) at the morphological and gene expression levels. Herein, we investigated the effect of EBFC-generated electrical stimulation on hAD-MSC cell morphology and gene expression using next-generation RNA sequencing. We tested three different electrical currents, 127 ± 9, 248 ± 15, and 598 ± 75 nA/cm2, in mesenchymal stem cells. We performed transcriptome profiling to analyze the impact of EBFC-derived electrical current on gene expression using next generation sequencing (NGS). We also observed changes in cytoskeleton arrangement and analyzed gene expression that depends on the electrical stimulation. The electrical stimulation of EBFC changes cell morphology through cytoskeleton re-arrangement. In particular, the results of whole transcriptome NGS showed that specific gene clusters were up- or down-regulated depending on the magnitude of applied electrical current of EBFC. In conclusion, this study demonstrates that EBFC-generated electrical stimulation can influence the morphological and gene expression properties of stem cells; such capabilities can be useful for regenerative medicine applications such as bioelectronic devices.

scholarly journals Combinatorial expression of cell cycle regulators is more suitable for immortalization than oncogenic methods in dermal papilla cells

iScience ◽  
2021 ◽  
Vol 24 (1) ◽  
pp. 101929
Author(s):  
Tomokazu Fukuda ◽  
Kai Furuya ◽  
Kouhei Takahashi ◽  
Ai Orimoto ◽  
Eriko Sugano ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Dermal Papilla ◽  
Cell Cycle Regulators ◽  
Dermal Papilla Cells ◽  
Combinatorial Expression

URB expression in human dermal papilla cells

2005 ◽  
Vol 39 (2) ◽  
pp. 128-130 ◽  
Author(s):  
So-Young Cha ◽  
Young Kwan Sung ◽  
Sanguk Im ◽  
Mi Hee Kwack ◽  
Moon Kyu Kim ◽  
...  
Keyword(s):  
Dermal Papilla ◽  
Dermal Papilla Cells
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