Bioethanol production using carob pod, as carbon source on submerged fermentation

2010 ◽  
Vol 150 ◽  
pp. 170-170 ◽  
Author(s):  
M.E. Lima-Costa ◽  
C. Tavares ◽  
B. Rodrigues ◽  
C. Quintas ◽  
S. Raposo
Keyword(s):  
Carbon Source ◽  
Submerged Fermentation ◽  
Bioethanol Production ◽  
Carob Pod

scholarly journals Studies on Nisin Production by Isolated Lactobacillus Species in Aqueous Two-phase System

2007 ◽  
Vol 6 (1) ◽  
pp. 69-80
Author(s):  
Namita Singh ◽  
S. K. Mandal
Keyword(s):  
Carbon Source ◽  
Submerged Fermentation ◽  
Biomass Yield ◽  
Cheese Whey ◽  
Maximum Yield ◽  
Lactobacillus Species ◽  
Phase System ◽  
Two Phase ◽  
Nisin Production ◽  
Aqueous Two Phase System

Isolated lactobacillus sp.(from cheese whey) was examined for its ability to produce bacteriocin like nisin in submerged fermentation and aqueous two-phase system(ATPS) at pH 6.5, 30.C for 18 h fermentation using M17S medium with 5%(v/v) inoculum and the nisin yields were obtained 4500 IU/ml and 20216 IU/ml where as biomass yield obtained 19.55 mg/ml and 31.88 mg/ml respectively.The maximum yield of nisin (24800 IU/ml) and biomass (22.49 mg/ml) were obtained in ATPS (28% PEG 6000 and 4% MgSO4.7H20 with 5-times concentrated M17 medium containing 0.5% lactose as carbon source) under similar conditions.

scholarly journals Exploring Simplified Methods for Insect Chitin Extraction and Application as a Potential Alternative Bioethanol Resource

Insects ◽  
2020 ◽  
Vol 11 (11) ◽  
pp. 788 ◽  
Author(s):  
Mahmoud Kamal ◽  
Eslam Adly ◽  
Sulaiman Ali Alharbi ◽  
Amany Soliman Khaled ◽  
Magda Hassan Rady ◽  
...  
Keyword(s):  
Submerged Fermentation ◽  
Fossil Fuels ◽  
Periplaneta Americana ◽  
Mucor Circinelloides ◽  
American Cockroach ◽  
Bioethanol Production ◽  
Chitin Extraction ◽  
Potential Alternative ◽  
Immense Potential ◽  
Insect Biomass

Chitin, the second most plentiful biopolymer in nature, is a major component of insect cuticle. In searching for alternative resources for fossil fuels, some fungal strains of Mucor circinelloides from an insect-source were found to produce bioethanol directly using insect chitin as a substrate. Herein, simplified methods for insect chitin extraction and application as a substrate in submerged fermentation for bioethanol production were explored. Chitin of the American cockroach (Periplaneta americana (L.)) was isolated by refluxing the cockroaches dried exoskeletons with 4% NaOH. The purity of the extracted chitin was assessed to be high when the physicochemical properties of the extracted chitin matched these of commercially available crab and shrimp samples. The extracted chitin was employed as a substrate in submerged fermentation using two strains of M. circinelloides. One of these, strains M. circinelloides 6017 showed immense potential for bioethanol production directly. It could to bio-transform 15 g/L of colloidal chitin directly to 11.22 ± 0.312 g/L of bioethanol (74% of the initial chitin mass) after 6 days of incubation. These results confirm the possibility of using insect biomass as a potential alternative resource for bioethanol production in a simple manner thus contributing to the creation of an alternate energy source.

scholarly journals N-Acetylglucosamine Utilization by Saccharomyces cerevisiae Based on Expression of Candida albicans NAG Genes

2009 ◽  
Vol 75 (18) ◽  
pp. 5840-5845 ◽  
Author(s):  
Jürgen Wendland ◽  
Yvonne Schaub ◽  
Andrea Walther
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Candida Albicans ◽  
Carbon Source ◽  
Sole Carbon Source ◽  
De Novo ◽  
Biofuel Production ◽  
Bioethanol Production ◽  
Catabolic Pathway ◽  
Cellular Functions ◽  
Kinase A

ABSTRACT Synthesis of chitin de novo from glucose involves a linear pathway in Saccharomyces cerevisiae. Several of the pathway genes, including GNA1, are essential. Genes for chitin catabolism are absent in S. cerevisiae. Therefore, S. cerevisiae cannot use chitin as a carbon source. Chitin is the second most abundant polysaccharide after cellulose and consists of N-acetylglucosamine (GlcNAc) moieties. Here, we have generated S. cerevisiae strains that are able to use GlcNAc as a carbon source by expressing four Candida albicans genes (NAG3 or its NAG4 paralog, NAG5, NAG2, and NAG1) encoding a GlcNAc permease, a GlcNAc kinase, a GlcNAc-6-phosphate deacetylase, and a glucosamine-6-phosphate deaminase, respectively. Expression of NAG3 and NAG5 or NAG4 and NAG5 in S. cerevisiae resulted in strains in which the otherwise-essential ScGNA1 could be deleted. These strains required the presence of GlcNAc in the medium, indicating that uptake of GlcNAc and its phosphorylation were achieved. Expression of all four NAG genes produced strains that could use GlcNAc as the sole carbon source for growth. Utilization of a GlcNAc catabolic pathway for bioethanol production using these strains was tested. However, fermentation was slow and yielded only minor amounts of ethanol (approximately 3.0 g/liter), suggesting that fructose-6-phosphate produced from GlcNAc under these conditions is largely consumed to maintain cellular functions and promote growth. Our results present the first step toward tapping a novel, renewable carbon source for biofuel production.

scholarly journals Promotion of the Hypocrellin Yield by a Co-Culture of Shiraia bambusicola (GDMCC 60438) with Arthrinium sp. AF-5 Fungus

Fermentation ◽  
2021 ◽  
Vol 7 (4) ◽  
pp. 316
Author(s):  
Xiaoxiao Yan ◽  
Yongdi Wen ◽  
Menghua Hu ◽  
Zhenqiang Wu ◽  
Xiaofei Tian
Keyword(s):  
Carbon Source ◽  
Submerged Fermentation ◽  
Microscope Observation ◽  
Time Increase ◽  
Anthracene Derivative ◽  
Shiraia Bambusicola ◽  
Hypocrellin A ◽  
Cultivation Strategy ◽  
Fermentation Media

Hypocrellin is a natural 3,10-xylene-4,9-anthracene derivative compound that originates from the stroma of Shiraia bambusicola (S. bambusicola) and Hypocrella bambusae with excellent photobiological activities. Submerged fermentation with the mycelia of S. bambusicola is generally regarded as an ideal technology for hypocrellin production. This study developed a co-cultivation strategy for an obvious promotion of the hypocrellin yield by incubating S. bambusicola (GDMCC 60438) with the endophyte fungus Arthrinium sp. AF-5 isolated from the bamboo tissue. The results indicated that the yield of hypocrellin A (HA) reached a 66.75 mg/g carbon source after an 84-h co-cultivation of the two strains, which was a four-time increase of that by the fermentation only with the S. bambusicola. The microscope observation found that the mycelia of the two strains were intertwined with each other to form the mycelium pellets during the co-cultivation. Moreover, the mycelium pellets of the co-culture showed a contracted and slightly damaged morphology. The addition of H2O2 in the fermentation media could further increase the HA production by 18.31%.

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