Quantitative analysis of gliding between subcutaneous tissue and the vastus lateralis – Influence of the dense connective tissue of the myofascia

2020 ◽  
Vol 24 (4) ◽  
pp. 316-320
Author(s):  
Kengo Kawanishi ◽  
Shintarou Kudo
Keyword(s):  
Quantitative Analysis ◽  
Connective Tissue ◽  
Subcutaneous Tissue ◽  
Vastus Lateralis ◽  
Dense Connective Tissue

scholarly journals Subcutaneous tissue response of isogenic mice to calcium hydroxide-based pastes with chlorhexidine

2009 ◽  
Vol 20 (2) ◽  
pp. 99-106 ◽  
Author(s):  
Raquel Assed Bezerra da Silva ◽  
Sada Assed ◽  
Paulo Nelson-Filho ◽  
Léa Assed Bezerra da Silva ◽  
Alberto Consolaro
Keyword(s):  
Quantitative Analysis ◽  
Connective Tissue ◽  
Calcium Hydroxide ◽  
Inflammatory Infiltrate ◽  
Subcutaneous Tissue ◽  
Fibrous Tissue ◽  
Fiber Formation ◽  
Tissue Response ◽  
Significant Difference ◽  
Subcutaneous Connective Tissue

This study was evaluated the response of subcutaneous connective tissue of isogenic mice to calcium hydroxide-based pastes with chlorhexidine digluconate (CHX). Seventy isogenic male BALB/c mice aged 6-8 weeks and weighing 15-20 g were randomly assigned to 8 groups. The animals received polyethylene tube implants as follows: Groups I, II, and III (n=10) - Calen® paste mixed with 0.4% CHX (experimental paste; Calen/CHX) for 7, 21, and 63 days, respectively; Groups IV, V, and VI (n=10) - UltraCal™ paste mixed with 2% CHX (experimental paste supplied by Ultradent Products Inc.; Ultracal/CHX) for 7, 21, and 63 days, respectively; and Groups VII and VIII (n=5): empty tube for 7 and 21 days, respectively. At the end of the experimental periods, the implants were removed together with the surrounding tissues (skin and subcutaneous connective tissue). The biopsied tissues were subjected to routine processing for histological analysis. Using a descriptive analysis and a four-point (0-3) scoring system, the following criteria were considered for qualitative and quantitative analysis of the tissue around the implanted materials: collagen fiber formation, tissue thickness and inflammatory infiltrate. A quantitative analysis was performed by measuring the thickness (µm), area (µm²) and perimeter (µm) of the reactionary granulomatous tissue formed at the tube ends. Data were analyzed statistically by the Kruskal-Wallis test and Dunn's post-test (α=0.05). Calen/CHX showed biocompatibility with the subcutaneous and reactionary tissues, with areas of discrete fibrosis and normal conjunctive fibrous tissue, though without statistically significant difference (p>0.05) from the control groups. In Groups I to III, there was a predominance of score 1, while in Groups IV to VI scores 2 and 3 predominated for all analyzed parameters. UltraCal/CHX, on the other hand, induced the formation of an inflammatory infiltrate and abundant exudate, suggesting a persistent residual aggression from the material, even 63 days after implant placement. In conclusion, the Calen paste mixed with 0.4% CHX allowed an adequate tissue response, whereas the UltraCal paste mixed with 2% CHX showed unsatisfactory results.

Biological Response to Wollastonite Doped α-Tricalcium Phosphate Implants in Hard and Soft Tissues in Rats

2008 ◽  
Vol 396-398 ◽  
pp. 7-10 ◽  
Author(s):  
Ana Maria Minarelli Gaspar ◽  
Sybele Saska ◽  
R. García Carrodeguas ◽  
A.H. De Aza ◽  
P. Pena ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Bone Formation ◽  
Bone Tissue ◽  
Tricalcium Phosphate ◽  
Rattus Norvegicus ◽  
Soft Tissues ◽  
Subcutaneous Tissue ◽  
Biological Response ◽  
New Bone Formation ◽  
Rat Bone

The biological response following subcutaneous and bone implantation of β-wollastonite(β-W)-doped α-tricalcium phosphate bioceramics in rats was evaluated. Tested materials were: tricalcium phosphate (TCP), consisting of a mixture of α- and β-polymorphs; TCP doped with 5 wt. % of β-W (TCP5W), composed of α-TCP as only crystalline phase; and TCP doped with 15 wt. % of β-W (TCP15), containing crystalline α-TCP and β-W. Cylinders of 2x1 mm were implanted in tibiae and backs of adult male Rattus norvegicus, Holtzman rats. After 7, 30 and 120 days, animals were sacrificed and the tissue blocks containing the implants were excised, fixed and processed for histological examination. TCP, TCP5W and TCP15W implants were biocompatible but neither bioactive nor biodegradable in rat subcutaneous tissue. They were not osteoinductive in connective tissue either. However, in rat bone tissue β-W-doped α-TCP implants (TCP5W and TCP15W) were bioactive, biodegradable and osteoconductive. The rates of biodegradation and new bone formation observed for TCP5W and TCP15W implants in rat bone tissue were greater than for non-doped TCP.

scholarly journals Histological analysis of the association between formocresol and endotoxin in the subcutaneous tissue of mice

2008 ◽  
Vol 19 (1) ◽  
pp. 40-45 ◽  
Author(s):  
Ana Teresa Sant'anna ◽  
Luis Carlos Spolidório ◽  
Lizeti Toledo Oliveira Ramalho
Keyword(s):  
Connective Tissue ◽  
Chronic Inflammation ◽  
Histological Analysis ◽  
Subcutaneous Tissue ◽  
Experimental Period ◽  
Connective Tissues ◽  
Plastic Tube ◽  
Tissue Samples ◽  
Subcutaneous Connective Tissue ◽  
Original Formula

This study performed a histological analysis of the effect of formocresol associated to endotoxin (LPS) in the subcutaneous connective tissue of mice. Ninety mice were randomly assigned to 3 groups (n=30). Each animal received one plastic tube implant containing endotoxin solution (10 mg/mL), formocresol (original formula) or a mixture of endotoxin and formocresol. The endotoxin and formocresol groups served as controls. The periods of analysis were 7, 15 and 30 days. At each experimental period, tissue samples were collected and submitted to routine processing for histological analysis. Endotoxin and formocresol produced necrosis and chronic inflammation at 7 and 15 days. At 30 days, the endotoxin group showed no necrosis, while in the formocresol group necrosis persisted. The formocresol-endotoxin association produced necrosis and chronic inflammation in the same way as observed with formocresol at all experimental periods. In conclusion, formocresol seems not to be able to inactive the toxic effects of endotoxin in connective tissues.

scholarly journals Existence and Features of the Myodural Bridge in Gentoo Penguins: a morphological study

2020 ◽  
Author(s):  
Cheng Chen ◽  
Sheng-bo Yu ◽  
Yan-yan Chi ◽  
Guang-yuan Tan ◽  
Bao-cheng Yan ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Dura Mater ◽  
Type I Collagen ◽  
Morphological Characteristics ◽  
Gentoo Penguin ◽  
Type I ◽  
Marine Birds ◽  
Dense Connective Tissue ◽  
Myodural Bridge ◽  
Histological Staining

AbstractRecent studies have evidenced that the anatomical structure now known as the myodural bridge (MDB) connects the suboccipital musculature to the cervical spinal dura mater (SDM). In humans, the MDB passes through both the posterior atlanto-occipital and the posterior atlanto-axial interspaces. The present authors suggest that the MDB has important physiological functions in humans. The existence of the MDB in various mammals, including flying birds (Rock pigeons and Gallus domesticus) has been previously validated. Gentoo penguins are marine birds, able to make 450 dives per day, reaching depths of up to 660 feet. Gentoo penguins are also the world’s fastest diving birds. The present study was therefore carried out to investigate the existence and characteristics of the MDB in Gentoo penguin (Pygoscelis papua), a non-flying, marine bird that can dive. While foraging, this penguin is able to reach speeds of up to 22 miles per hour. For this study, six Gentoo penguin specimens were dissected to observe the existence and composition of their MDB. Histological staining was also performed to analyze the anatomic relationships and characteristic of the MDB in the Gentoo penguin. In this study, it was found that the suboccipital musculature in the Gentoo penguin consists of the rectus capitis dorsalis minor (RCDmi) muscle and rectus capitis dorsalis major (RCDma) muscle. Dense connective tissue fibers were observed connecting these two suboccipital muscles to the spinal dura mater (SDM). This dense connective tissue bridge consists of primarily type I collagen fibers. Thus, this penguin’s MDB appears to be analogous to the MDB previously observed in humans. The present study evidences that the MDB not only exists in penguins but it also has unique features that distinguishes it from that of flying birds. Thus, this study advances the understanding of the morphological characteristics of the MDB in flightless, marine birds.

scholarly journals PATHOMORPHOLOGIC CHARACTERISTICS OF PALMAR APONEUROSIS IN PATIENTS WITH DUPUYTREN’S CONTRACTURE AND VIRAL HEPATITIS

2021 ◽  
Vol 29 (4) ◽  
pp. 454-461
Author(s):  
T.A. Stupina ◽  
◽  
T.N. Varsegova ◽  
Keyword(s):  
Hepatitis B ◽  
Connective Tissue ◽  
Viral Hepatitis ◽  
Nerve Fibers ◽  
Control Group ◽  
Dupuytren’S Contracture ◽  
Virus Hepatitis ◽  
Dense Connective Tissue ◽  
Dupuytren's Contracture ◽  
Palmar Aponeurosis

Objective. To establish pathomorphologic peculiarities of palmar aponeurosis in the patients with Dupuytren’s contracture and concomitant virus hepatitis B and C. Methods. The data analysis of histomorphometric studies of the operation samples of 122 patients with Dupuytren’s contracture («Control» group, n=100) and Dupuytren’s contracture with virus hepatitis B and C («Hepatitis» group, n=22) were analyzed. Results. In patients of the «Hepatitis» group, the content of adiposed tissue in the palmar aponeurosis was 40.9% less (p <0.01) than in the «Control» group, and the content of dense connective tissue was 18.9% higher (p <0, 05). In all patients, arteries with the diameter up to 150 µm prevailed in the palmar aponeurosis, but in the «Hepatitis» group their percentage was reduced by 20%, with higher proportions of vessels with the diameter of 150-450 µm and the absence or recalibration of the largest arteries. In the «Control» group, the arteries had diameters from 50 to 660 µm, in the «Hepatitis» group they did not exceed 370 µm. Vessels less than 300 µm in diameter in both groups had comparable values of the Kernogan’s index. Arteries with a diameter of more than 300 microns in the «Hepatitis» group had higher values of the Kernogan’s index, which indicated a low conductance capacity compared to the vessels in the «Control» group. Most of the nerve trunks of the palmar aponeurosis in the patients of «Hepatitis» group showed signs of necrobiotic changes, i.e. fibrotic or swollen perineurium, withinflammatory cell infiltration, sometimes lost lamellar structure, and nerve fibers with signs of Wallerian degeneration. Conclusion. Obtained histomorphometric data of the tissue composition of palmar fascial fibromatosis, less amount of adiposed tissue and higher amount of dense connective one and more pronounced disorder of hemodynamics and innervation of the palmar aponeurosis in the group with concomitant virus hepatitis indicate significant effect of the liver pathology on the progression of the disease. What this paper adds For the first time, the pathomorphological features of the palmar aponeurosis have been studied in 122 patients with Dupuytren’s contracture and concomitant viral hepatitis B and C.It has been found that in patients with Dupuytren’s contracture and hepatitis in the palmar aponeurosis, the amount of adiposed tissue is reduced and the amount of dense connective tissue is increased; hemodynamic and innervation disorders are more pronounced.

Internal Structural Connections in the Popliteal Muscle Tendon Complex and Their Clinical Significance

2021 ◽  
Author(s):  
WenBin Jiang ◽  
Shi-Zhu Sun ◽  
Ting-Wei Song ◽  
Chan Li ◽  
Wei Tang ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Knee Joint ◽  
Tibial Plateau ◽  
Lateral Meniscus ◽  
Posterior Edge ◽  
Dense Connective Tissue ◽  
Human Knee ◽  
Articular Capsule ◽  
Adjacent Structures ◽  
Popliteus Muscle

Abstract Background:The popliteal muscle-tendon complex (PMTC) belongs to the deep structure of the posterolateral complex (PLC) of human knee, which plays an important role in the posterolateral stability of the knee joint. At present, the anatomical relationship between the popliteal muscle and its adjacent structures remains controversial, especially the posterior cruciate ligament (PCL) and popliteal muscle. The revealation of anatomical connection between the popliteus muscle and its deep structures could provide an anatomical basis for the reconstruction of the PLC injury.Methods: To observe and analyze the relationship between popliteal muscle and the PCL, posterior meniscofemoral ligament (PMFL), lateral meniscus and articular capsule (AC). The dissection of 7 cases of adult human knee joint fixed with formalin, and 9 cases of sagittal P45 plastinated section of the knee joint were involved in this study. Results: For the popliteal muscle, the anatomical dissection showed that at the posterior edge of the platform of the lateral condyle of the tibia, at the tendon-muscle transition, from medial to lateral, separately sent out: dense connective tissue to connect with the PCL, dense fiber bundles to connect with the PMFL, and dense connective tissue band to connect the lateral meniscus. Meanwhile, the results of the P45 section revealed that the popliteal muscle fascia ran superiorly over the posterior edge of the tibialintercondylar eminence, andturned forward to be integrated into the PCL. Laterally, near the posterior edge of the lateral tibial plateau, the popliteal tendon penetrates through the articular capsule, where two dense fiberous bundleswere given off upwards by the popliteal tendon: one was the ventral fiber bundle,which ran superiorly over the posterior edge of the tibial plateau and then moved forwards to connect with the lateral meniscus; the dorsal fibersbundle ascended directly and participated in the AC.Conclusion: Popliteus muscle was connected with PCL, AC, lateral meniscus, and PMFL via the dense connective tissues near its tendon-muscle transition.

Sign in / Sign up

Export Citation Format

Share Document