Nano lipid based carriers for lymphatic voyage of anti-cancer drugs: An insight into the in-vitro, ex-vivo, in-situ and in-vivo study models

The high attrition rates of anti-cancer drugs during clinical development remains a bottleneck problem in pharmaceutical industry. This is partially due to the lack of quantitative, selective, and rapid readouts of anti-cancer drug activity in situ with high resolution. Although fluorescence microscopy has been commonly used in oncology pharmacological research, fluorescent labels are often too large in size for small drug molecules, and thus may disturb the function or metabolism of these molecules. Such challenge can be overcome by coherent Raman scattering microscopy, which is capable of chemically selective, highly sensitive, high spatial resolution, and high-speed imaging, without the need of any labeling. Coherent Raman scattering microscopy has tremendously improved the understanding of pharmaceutical materials in the solid state, pharmacokinetics of anti-cancer drugs and nanocarriers in vitro and in vivo. This review focuses on the latest applications of coherent Raman scattering microscopy as a new emerging platform to facilitate oncology pharmacokinetic research.

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pH-Sensitive Ratiometric Fluorescent Probe for Evaluation of Tumor Treatments

Materials ◽

10.3390/ma12101632 ◽

2019 ◽

Vol 12 (10) ◽

pp. 1632

Author(s):

Peisen Zhang ◽

Junli Meng ◽

Yingying Li ◽

Zihua Wang ◽

Yi Hou

Keyword(s):

Ph Sensitive ◽

Fluorescent Imaging ◽

Tat Peptide ◽

Cancer Drugs ◽

Ph Response ◽

In Vivo Experiments ◽

Anti Cancer ◽

Amidation Reaction

Determining therapeutic efficacy is critical for tumor precision theranostics. In order to monitor the efficacy of anti-cancer drugs (e.g., Paclitaxel), a pH-sensitive ratiometric fluorescent imaging probe was constructed. The pH-sensitive ratiometric fluorescent dye ANNA was covalently coupled to the N-terminal of the cell-penetrating TAT peptide through an amidation reaction (TAT-ANNA). The in vitro cellular experiments determined that the TAT-ANNA probe could penetrate the cell membrane and image the intracellular pH in real time. The in vivo experiments were then carried out, and the ratiometric pH response to the state of the tumor was recorded immediately after medication. The TAT-ANNA probe was successfully used to monitor the pharmacodynamics of anti-cancer drugs in vivo.

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In vivo and in vitro biocompatibility study of MnFe2O4 and Cr2Fe6O12 as photosensitizer for photodynamic therapy and drug delivery of anti-cancer drugs

Drug Development and Industrial Pharmacy ◽

10.1080/03639045.2020.1757698 ◽

2020 ◽

Vol 46 (5) ◽

pp. 846-851

Author(s):

Mozhgan Aghajanzadeh ◽

Ehsan Naderi ◽

Mostafa Zamani ◽

Ali Sharafi ◽

Mahmoud Naseri ◽

...

Keyword(s):

Drug Delivery ◽

Photodynamic Therapy ◽

Cancer Drugs ◽

In Vitro Biocompatibility ◽

Anti Cancer ◽

Biocompatibility Study

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Potentiation of the Anti-Proliferative Effects of Anti-Cancer Drugs by Octreotide in vitro and in vivo

Digestion ◽

10.1159/000201388 ◽

1996 ◽

Vol 57 (1) ◽

pp. 22-28 ◽

Cited By ~ 22

Author(s):

G. Weckbecker ◽

F. Raulf ◽

L. Tolcsvai ◽

C. Bruns

Keyword(s):

Cancer Drugs ◽

Anti Cancer

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Intracellular concentrations of anti cancer drugs in leukemic cells in vitro vs in vivo

Cancer Chemotherapy and Pharmacology ◽

10.1007/bf00684881 ◽

1990 ◽

Vol 25 (4) ◽

pp. 252-256 ◽

Cited By ~ 18

Author(s):

B. Sundman-Engberg ◽

U. Tidefelt ◽

J. Liliemark ◽

C. Paul

Keyword(s):

Leukemic Cells ◽

Cancer Drugs ◽

Anti Cancer ◽

Intracellular Concentrations

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Protective Effect of Pirenoxine and U74389F on Induced Lipid Peroxidation in Mammalian Lenses. An in vitro, ex vivo and in vivo study

Experimental Eye Research ◽

10.1006/exer.1998.0612 ◽

1999 ◽

Vol 68 (3) ◽

pp. 347-359 ◽

Cited By ~ 9

Author(s):

MARIO CIUFFI ◽

SILVIA NERI ◽

SERGIO FRANCHI-MICHELI ◽

PAOLA FAILLI ◽

LUCILLA ZILLETTI ◽

...

Keyword(s):

Lipid Peroxidation ◽

Protective Effect ◽

Ex Vivo ◽

In Vivo Study

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In Situ Gels of Acylovir Nanoemulsions For Improved Delivery to The Eye

Drug Delivery Letters ◽

10.2174/2210303111666210812160624 ◽

2021 ◽

Vol 11 ◽

Author(s):

Manza M. Priyanka ◽

Shinde A. Ujwala ◽

Sheth M. Kalyani ◽

Namita Desai

Keyword(s):

Animal Studies ◽

Ex Vivo ◽

In Vitro Release ◽

Corneal Stroma ◽

Class Iii ◽

Ophthalmic Delivery ◽

Histopathological Studies

Background: Acyclovir, BCS Class III drug is commercially available as 3 % w/w eye ointment for multiple applications. Acyclovir nanoemulsions can be proposed to reduce dose because of improved permeation characteristics. Further, the development of in situ ophthalmic gels can be advantageous to reduce the number of applications due to increased mucoadhesion and sustaining effect. Objective: The purpose of this study was the development and evaluation of nanoemulsions based in situ gels of Acyclovir (1% w/w) as potential ophthalmic delivery systems. Methods: Nanoemulsions of Acyclovir were developed by Phase Inversion Temperature method using Capmul MCM, stearyl amine and Kolliphor RH 40 as liquid lipid, charge inducer and surfactant, respectively selected on the basis of Acyclovir solubility studies in the oil phase and emulsification ability of surfactants. These nanoemulsions were further developed into in situ ophthalmic gels using gellan gum and Methocel K4M. Results: The developed gels showed a sustained effect in vitro release studies and improved goat corneal permeation in ex vivo studies when compared to marketed ointment. HET-CAM studies concluded the absence of irritation potential, while in vivo irritation study in Wistar rats showed the absence of erythema and swelling of eyes after visual inspection for 72 hours. Histopathological studies on isolated rat corneas showed no abnormalities in anterior corneal epithelium and corneal stroma without any epithelial hyperplasia. Acyclovir nanoemulsions based in situ ophthalmic gel showed increased corneal deposition and permeation in rat eyes. Conclusion: The improved potential of developed ophthalmic gels was proven due to the reduced frequency of application compared to the marketed ointment in animal studies.

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