Calcium glycerophosphate and fluoride affect the pH and inorganic composition of dual-species biofilms of Streptococcus mutans and Candida albicans

Introduction: This study aimed to evaluate the antibacterial and antifungal effect of Ag and zinc oxide nanoparticles to complete denture acrylic resin. Materials & Methods: In this experimental laboratory study, 144 samples were evaluated. The powder of acrylic resin (PMMA Ivoclar Vivadent, Lichenstein SR Triplex Hot) was mixed homogeneously in the ultrasonic apparatus with Ag (purity: 99.99%, size: 20nm, spherical) and ZnO (purity: 99%, size: 10-30 nm, nearly spherical) particles with mass fraction 0.5, 1 ,2 and 5. The microorganisms of this study were Candida albicans and Streptococcus mutans. The growth rate of microorganisms was measured by spectrophotometer based on turbidity and readings at 600 nm. Data were analyzed by ANOVA and Tukey post hoc tests. The significance level was set at 0.05. Results: Increasing Ag and ZnO nanoparticles to acrylic resin reduced the growth of Candida Albicans and Streptococcus Mutans. Even the concentration of 0.5% significantly reduced the growth of microorganisms. Complete growth inhibition was in the concentration of 5% after 24 and 48 hours. Conclusion: Increasing the Ag and ZnO nanoparticles to acrylic resin reduced the growth of microorganisms.

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Atividade antimicrobiana in vitro da fração obtida do óleo de Pterodon pubescens contra Candida spp., Streptococcus mutans, Staphylococcus aures e biofilme de Candida albicans

10.47749/t/unicamp.2013.918882 ◽

2013 ◽

Author(s):

Fabiana Paganotti Roque

Keyword(s):

Candida Albicans ◽

Streptococcus Mutans ◽

Candida Spp

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In vitro antimicrobial activity of mineral trioxide aggregate, Biodentine, and calcium-enriched mixture cement against Enterococcus faecalis, Streptococcus mutans, and Candida albicans using the agar diffusion technique

Dental Research Journal ◽

10.4103/1735-3327.310032 ◽

2021 ◽

Vol 18 (1) ◽

pp. 3

Author(s):

MaryamZare Jahromi ◽

Parinaz Esteki ◽

Arezoo Tahmourespour

Keyword(s):

Antimicrobial Activity ◽

Candida Albicans ◽

Streptococcus Mutans ◽

Enterococcus Faecalis ◽

Mineral Trioxide Aggregate ◽

In Vitro Antimicrobial Activity ◽

Agar Diffusion ◽

Diffusion Technique

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Relationship between Candida albicans and Streptococcus mutans in early childhood caries, evaluated by quantitative PCR

F1000Research ◽

10.12688/f1000research.16275.1 ◽

2018 ◽

Vol 7 ◽

pp. 1645 ◽

Cited By ~ 7

Author(s):

Endang W. Bachtiar ◽

Boy M. Bachtiar

Keyword(s):

Early Childhood ◽

Candida Albicans ◽

Streptococcus Mutans ◽

Dental Plaque ◽

Early Childhood Caries ◽

Positive Correlation ◽

Unstimulated Saliva ◽

Student’S T ◽

Total Bacteria ◽

Childhood Caries

Background:The aim of this study was to analyze the synergistic relationship betweenCandida albicansandStreptococcus mutansin children with early childhood caries (ECC) experience.Methods:Dental plaque and unstimulated saliva samples were taken from 30 subjects aged 3-5 years old, half with (n=15, dmft > 4) and half without (n=15) ECC. The abundance ofC. albicansandS. mutansand relative to total bacteria load were quantify by real-time PCR (qPCR). This method was also employed to investigate the mRNA expression of glycosyltransferase (gtfB) gene in dental plaque. Student’s t-test and Pearson’s correlation were used to perform statistical analysis.Results:Within the ECC group, the quantity of both microorganisms were higher in the saliva than in dental plaque. The ratio ofC. albicansto total bacteria was higher in saliva than in plaque samples (p < 0.05). We observed the opposite forS. mutans(p < 0.05). The different value ofC. albicansandS. mutansin saliva was positively correlated, and negatively correlated in dental plaque. Transcription level ofS. mutans gtfBshowed a positive correlation withC. albicansconcentration in dental plaque. Conclusion:C. albicanshas a positive correlation with cariogenic traits ofS. mutansin ECC-related biofilm of young children.

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Association between Streptococcus mutans and Candida albicans among 3–5-year-old children with early childhood caries: A cross-sectional study

Journal of Indian Association of Public Health Dentistry ◽

10.4103/jiaphd.jiaphd_80_17 ◽

2017 ◽

Vol 15 (3) ◽

pp. 205

Author(s):

F Farhanaz ◽

R Yashoda ◽

ML Kalayadhav ◽

ManjunathP Puranik

Keyword(s):

Early Childhood ◽

Candida Albicans ◽

Streptococcus Mutans ◽

Early Childhood Caries ◽

Cross Sectional Study ◽

Sectional Study ◽

Cross Sectional ◽

Childhood Caries

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AÇÃO ANTIMICROBIANA DO Zingiber officinalle FRENTE À MICROBIOTA BUCAL

Estudos de Biologia ◽

10.7213/reb.v28i62.22719 ◽

2006 ◽

Vol 28 (62) ◽

Cited By ~ 2

Author(s):

Ana Maria Trindade Grégio ◽

Edilene Sayuri Miyamoto Fortes ◽

Edvaldo A. Ribeiro Rosa ◽

Rossana Baggio Simeoni ◽

Rosemeire Takaki Rosa

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Candida Albicans ◽

Streptococcus Mutans

A fitoterapia tem sido alvo de investigações científicas, especialmente em países do oriente. No Brasil, também ocorre um especial interesse da comunidade científica quanto ao uso, efeito e propriedades farmacológicas das plantas medicinais, tendo o seu uso direcionado ao tratamento de várias doenças. A microbiota bucal é um dos locais em que há uma maior variedade de microrganismos que podem, por um desequilíbrio, tornar-se patogênicos. Baseado nisso, um estudo do extrato da Zingiber officinalle foi realizado para avaliar o seu potencial antimicrobiano, com microrganismos comumente encontrados na cavidade bucal, no qual foi obtida a concentração mínima inibitória do extrato de gengibre frente a alguns tipos de patógenos presentes na microbiota bucal do ser humano. Foi utilizado o método microbiológico no qual os extratos glicólico e hidroalcoólico do gengibre sofreram diluições seriadas no meio de cultura (BHI) para uma posterior inoculação dos microrganismos, Streptococcus mutans, Staphylococcus aureus, Escherichia coli e Candida albicans. Obteve-se a concentração mínima inibitória dos extratos de gengibre, tanto na sua forma glicólica quanto hidroalcoólica. A dose mínima necessária para inibir o crescimento dos vários microrganismos testados foi de 5mg/ml dos dois extratos estudados. O extrato da Zingiber officinalle apresentou atividade antibacteriana e antifúngica relevante para a Odontologia, podendo contribuir para o tratamento de doenças causadas por esses microrganismos presentes na cavidade bucal.

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Synergism of Streptococcus mutans and Candida albicans Reinforces Biofilm Maturation and Acidogenicity in Saliva: An In Vitro Study

Frontiers in Cellular and Infection Microbiology ◽

10.3389/fcimb.2020.623980 ◽

2021 ◽

Vol 10 ◽

Author(s):

Hye-Eun Kim ◽

Yuan Liu ◽

Atul Dhall ◽

Marwa Bawazir ◽

Hyun Koo ◽

...

Keyword(s):

Candida Albicans ◽

Dental Caries ◽

Streptococcus Mutans ◽

Critical Role ◽

In Vitro Study ◽

Acidic Ph ◽

Acidic Environment ◽

Symbiotic Interaction ◽

Biofilm Development

Early childhood caries, a virulent-form of dental caries, is painful, difficult, and costly to treat that has been associated with high levels of Streptococcus mutans (Sm) and Candida albicans (Ca) in plaque-biofilms on teeth. These microorganisms appear to develop a symbiotic cross-kingdom interaction that amplifies the virulence of plaque-biofilms. Although biofilm studies reveal synergistic bacterial-fungal association, how these organisms modulate cross-kingdom biofilm formation and enhance its virulence in the presence of saliva remain largely unknown. Here, we compared the properties of Sm and Sm-Ca biofilms cultured in saliva by examining the biofilm structural organization and capability to sustain an acidic pH environment conducive to enamel demineralization. Intriguingly, Sm-Ca biofilm is rapidly matured and maintained acidic pH-values (~4.3), while Sm biofilm development was retarded and failed to create an acidic environment when cultured in saliva. In turn, the human enamel slab surface was severely demineralized by Sm-Ca biofilms, while there was minimal damage to the enamel surface by Sm biofilm. Interestingly, Sm-Ca biofilms exhibited an acidic environment regardless of their hyphal formation ability. Our data reveal the critical role of symbiotic interaction between S. mutans and C. albicans in human saliva in the context of pathogenesis of dental caries, which may explain how the cross-kingdom interaction contributes to enhanced virulence of plaque-biofilm in the oral cavity.

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