Protective effects of Paederia scandens extract on rheumatoid arthritis mouse model by modulating gut microbiota

Rheumatoid arthritis is emerging as a chronic autoimmune disease worldwide. In this study, the components of tuna oil (TO) were firstly measured, and the beneficial effects of TO on collagen-induced...

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The therapeutic use of osmotic minipumps in the severe combined immunodeficiency (SCID) mouse model for rheumatoid arthritis

Annals of the Rheumatic Diseases ◽

10.1136/ard.2007.086116 ◽

2008 ◽

Vol 68 (1) ◽

pp. 124-129 ◽

Cited By ~ 10

Author(s):

A Knedla ◽

B Riepl ◽

S Lefèvre ◽

S Kistella ◽

J Grifka ◽

...

Keyword(s):

Rheumatoid Arthritis ◽

Mouse Model ◽

Scid Mouse ◽

Severe Combined Immunodeficiency ◽

Protective Effects ◽

Combined Immunodeficiency ◽

Human Cartilage ◽

Osmotic Minipumps ◽

Osmotic Pumps ◽

Scid Mouse Model

Objectives:The viral gene transfer of interleukin 1 receptor antagonist (IL1ra) and interleukin 10 (IL10) into rheumatoid arthritis (RA) synovial fibroblasts (RASFs) has shown protective effects on cartilage destruction in the severe combined immunodeficiency (SCID) mouse model of RA. Nevertheless, side effects of viral transduction are possible and a number of cytokines or cytokine inhibitors are not available encoded in viral vehicles. As the production of viruses coding for bioactive proteins is cost and time intensive, we established an in vivo long-term release model using osmotic minipumps in the SCID mouse model for RA.Methods:Isolated RASFs were cultured for four passages and coimplanted together with human cartilage and an Alzet osmotic miniature pump model 2004, containing 200 μl of IL10 and IL1ra for 40 days in SCID mice. Implants were removed after 40 days and evaluated histologically. The actual rates of IL10 and IL1ra in murine serum were measured by ELISA.Results:Release of IL10 and IL1ra by the pumps was effective as both could be measured in significant amounts in the serum of the mice. IL10 and IL1ra release showed protective effects towards the coimplanted cartilage, similar to the adenovirally IL10/IL1ra-transduced RASFs. The mean (SD) invasion scores for the implants with the osmotic pumps were: invasion 0.7 (0.5), degradation 0.5 (0.3) (all parameters significant vs controls, p<0.05).Conclusions:The results demonstrate that the combination of osmotic pumps with the SCID mouse model for RA can be used as approach for application and evaluation of cartilage-protective molecules. Furthermore, the effect of cartilage-protective cytokines is independent of the type of application.

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Protective effects of SKLB023 on a mouse model of unilateral ureteral obstruction by the modulation of gut microbiota

RSC Advances ◽

10.1039/c8ra08049f ◽

2018 ◽

Vol 8 (70) ◽

pp. 40232-40242

Author(s):

Yanhuan Feng ◽

Lingzhi Li ◽

Fan Guo ◽

Yanping Li ◽

Yan Liang ◽

...

Keyword(s):

Mouse Model ◽

Gut Microbiota ◽

Ureteral Obstruction ◽

Renal Fibrosis ◽

Unilateral Ureteral Obstruction ◽

Protective Effects ◽

Common Pathway ◽

The Common

Renal fibrosis is the common pathway underlying the progression of CKD to ESRD and quantitative and qualitative alterations in gut microbiota are noted in patients with CKD. Our results indicated SKLB023 drives the alteration of gut microbiota to attenuate renal fibrosis.

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Modulation of Gut Microbiota by Lonicera caerulea L. Berry Polyphenols in a Mouse Model of Fatty Liver Induced by High Fat Diet

Molecules ◽

10.3390/molecules23123213 ◽

2018 ◽

Vol 23 (12) ◽

pp. 3213 ◽

Cited By ~ 23

Author(s):

Shusong Wu ◽

Ruizhi Hu ◽

Hironobu Nakano ◽

Keyu Chen ◽

Ming Liu ◽

...

Keyword(s):

Mouse Model ◽

Gut Microbiota ◽

Fatty Liver ◽

Relative Abundance ◽

High Fat Diet ◽

Rrna Gene ◽

Protective Effects ◽

High Fat ◽

Lonicera Caerulea ◽

Alcoholic Fatty Liver

Polyphenols from the Lonicera caerulea L. berry have shown protective effects on experimental non-alcoholic fatty liver disease (NAFLD) in our previous studies. As endotoxins from gut bacteria are considered to be the major trigger of inflammation in NAFLD, this study aims to clarify the regulatory effects of L. caerulea L. berry polyphenols (LCBP) on gut microbiota in a high fat diet (HFD)-induced mouse model. C57BL/6N mice were fed with a normal diet, HFD, or HFD containing 0.5–1% of LCBP for 45 days. The results revealed that supplementation with LCBP decreased significantly the levels of IL-2, IL-6, MCP-1, and TNF-α in serum, as well as endotoxin levels in both serum and liver in HFD-fed mice. Fecal microbiota characterization by high throughput 16S rRNA gene sequencing revealed that a HFD increased the Firmicutes/Bacteroidetes ratio, and LCBP reduced this ratio by increasing the relative abundance of Bacteroides, Parabacteroides, and another two undefined bacterial genera belonging to the order of Bacteroidales and family of Rikenellaceae, and also by decreasing the relative abundance of six bacterial genera belonging to the phylum Firmicutes, including Staphylococcus, Lactobacillus, Ruminococcus, and Oscillospira. These data demonstrated that LCBP potentially attenuated inflammation in NAFLD through modulation of gut microbiota, especially the ratio of Firmicutes to Bacteroidetes.

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Faculty Opinions recommendation of Protective effects of C-type natriuretic peptide on linear growth and articular cartilage integrity in an inflammatory arthritis mouse model.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.718139019.793492957 ◽

2014 ◽

Author(s):

Jason McDougall ◽

Eugene Krustev

Keyword(s):

Articular Cartilage ◽

Mouse Model ◽

Natriuretic Peptide ◽

Inflammatory Arthritis ◽

Linear Growth ◽

Protective Effects

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Regulatory effect of volatile compounds in fermented alcoholic beverages on gut microbiota and serum metabolism in mouse model

Food & Function ◽

10.1039/d0fo03028g ◽

2021 ◽

Author(s):

Mei Ji ◽

Cheng Fang ◽

Wei Jia ◽

Hai Du ◽

Yan Xu

Keyword(s):

Risk Factor ◽

Liver Disease ◽

Mouse Model ◽

Gut Microbiota ◽

Volatile Compounds ◽

Alcoholic Liver Disease ◽

Alcoholic Beverages ◽

Regulatory Effect ◽

Main Risk Factor

Ethanol (EtOH) is the main risk factor for alcoholic liver disease. However, fermented alcoholic beverages contain not only ethanol but also various volatile compounds. Currently, effects of volatile compounds in...

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Dietary Interventions to Prevent High Fructose Diet-associated Worsening of Colitis and Colitis-associated Tumorigenesis in Mice

Carcinogenesis ◽

10.1093/carcin/bgab007 ◽

2021 ◽

Author(s):

Ryohei Nishiguchi ◽

Srijani Basu ◽

Hannah A Staab ◽

Naotake Ito ◽

Xi Kathy Zhou ◽

...

Keyword(s):

Gut Microbiota ◽

Control Diet ◽

Experimental Colitis ◽

Protective Effects ◽

Diet Change ◽

Chronic Colitis ◽

Acute Colitis ◽

High Fructose Diet ◽

High Consumption ◽

High Fructose

Abstract Diet is believed to be an important factor in the pathogenesis of Inflammatory Bowel Disease. High consumption of dietary fructose has been shown to exacerbate experimental colitis, an effect mediated through the gut microbiota. This study evaluated whether dietary alterations could attenuate the detrimental effects of a high fructose diet (HFrD) in experimental colitis. First, we determined whether the pro-colitic effects of a HFrD could be reversed by switching mice from a HFrD to a control diet. This diet change completely prevented HFrD-induced worsening of acute colitis, in association with a rapid normalization of the microbiota. Second, we tested the effects of dietary fiber, which demonstrated that psyllium was the most effective type of fiber for protecting against HFrD-induced worsening of acute colitis, compared to pectin, inulin or cellulose. In fact, supplemental psyllium nearly completely prevented the detrimental effects of the HFrD, an effect associated with a shift in the gut microbiota. We next determined whether the protective effects of these interventions could be extended to chronic colitis and colitis-associated tumorigenesis. Using the azoxymethane/dextran sodium sulfate model, we first demonstrated that HFrD feeding exacerbated chronic colitis and increased colitis-associated tumorigenesis. Using the same dietary changes tested in the acute colitis setting, we also showed that mice were protected from HFrD-mediated enhanced chronic colitis and tumorigenesis, upon either diet switching or psyllium supplementation. Taken together, these findings suggest that high consumption of fructose may enhance colon tumorigenesis associated with long-standing colitis, an effect that could be reduced by dietary alterations.

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Polyherbal formula SC-E3 inhibits rheumatoid arthritis activity in a mouse model of type-II collagen-induced arthritis

Journal of Integrative Medicine ◽

10.1016/j.joim.2020.12.001 ◽

2020 ◽

Author(s):

Ju-Yeon Park ◽

Young-Won Kwon ◽

Sun-Ah Kim ◽

Sun-Dong Park ◽

Chang-Hyun Kim ◽

...

Keyword(s):

Rheumatoid Arthritis ◽

Mouse Model ◽

Type Ii Collagen ◽

Type Ii ◽

Collagen Induced Arthritis ◽

Rheumatoid Arthritis Activity

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Dietary resveratrol attenuation of intestinal inflammation and oxidative damage is linked to the alteration of gut microbiota and butyrate in piglets challenged with deoxynivalenol

Journal of Animal Science and Biotechnology ◽

10.1186/s40104-021-00596-w ◽

2021 ◽

Vol 12 (1) ◽

Author(s):

Yueqin Qiu ◽

Jun Yang ◽

Li Wang ◽

Xuefen Yang ◽

Kaiguo Gao ◽

...

Keyword(s):

Gut Microbiota ◽

Oxidative Damage ◽

Protein Expression ◽

Intestinal Inflammation ◽

Control Diet ◽

Intestinal Barrier Function ◽

Protective Effects ◽

Gut Health ◽

Antioxidant Genes ◽

Mrna And Protein Expression

Abstract Background Deoxynivalenol (DON) is a widespread mycotoxin that induces intestinal inflammation and oxidative stress in humans and animals. Resveratrol (RES) effectively exerts anti-inflammatory and antioxidant effects. However, the protective effects of RES on alleviating DON toxicity in piglets and the underlying mechanism remain unclear. Therefore, this study aimed to investigate the effect of RES on growth performance, gut health and the gut microbiota in DON-challenged piglets. A total of 64 weaned piglets [Duroc × (Landrace × Yorkshire), 21-d-old, 6.97 ± 0.10 kg body weight (BW)] were randomly allocated to 4 treatment groups (8 replicate pens per treatment, each pen containing 2 males; n = 16 per treatment) for 28 d. The piglets were fed a control diet (CON) or the CON diet supplemented with 300 mg RES/kg diet (RES group), 3.8 mg DON/kg diet (DON) or both (DON+RES) in a 2 × 2 factorial design. Results DON-challenged piglets fed the RES-supplemented diet had significantly decreased D-lactate concentrations and tumor necrosis factor alpha (TNF-α) and interleukin 1 beta (IL-1β) mRNA and protein expression, and increased zonula occludens-1 (ZO-1) mRNA and protein expression compared with those of DON-challenged piglets fed the unsupplemented diet (P < 0.05). Compared with unsupplemented DON-challenged piglets, infected piglets fed a diet with RES showed significantly decreased malondialdehyde (MDA) levelsand increased mRNA expression of antioxidant enzymes and antioxidant genes (i.e., GCLC, GCLM, HO-1, SOD1 and NQO-1) and glutamate-cysteine-ligase modulatory subunit (GCLM) protein expression (P < 0.05). Moreover, RES supplementation significantly abrogated the increase in the proportion of TUNEL-positive cells and the protein expression of caspase3 in DON-challenged piglets (P < 0.05). Finally, RES supplementation significantly increased the abundance of Roseburia and butyrate concentrations, while decreasing the abundances of Bacteroides and unidentified-Enterobacteriaceae in DON-challenged piglets compared with DON-challenged piglets alone (P < 0.05). Conclusions RES supplementation improved gut health in DON-challenged piglets by strengthening intestinal barrier function, alleviating intestinal inflammation and oxidative damage, and positively modulating the gut microbiota. The protective effects of RES on gut health may be linked to increased Roseburia and butyrate concentrations, and decreased levels of Bacteroides and unidentified-Enterobacteriaceae.

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