Antibacterial and anti-inflammatory activity of bistort (Bistorta officinalis) aqueous extract and its major components. Justification of the usage of the medicinal plant material as a traditional topical agent

Objective: The present study was designed to identify the bioactive phytochemicals and its antibacterial and in vitro anti-inflammatory potential of aqueous and methanolic seed extract of Citrullus lanatus.Methods: The phytochemical screening of both the aqueous and methanolic seed extract was carried out qualitatively to identify the major Phyto-constituents present in the extracts. The antimicrobial activity of the extracts was evaluated against six pathogenic bacterial strains by agar well diffusion method and the Minimum inhibitory concentration (MIC) was determined by broth dilution method. In vitro anti-inflammatory activity of C. lanatus seed extracts was evaluated by using human red blood cell (HRBC) membrane stabilization and inhibition of albumin denaturation method.Results: The results of the study indicated that both the extracts of the seed having antimicrobial activity, while the methanolic extract showed more significant activity against the tested organism than aqueous extract. Methanol extract had the lowest MIC of 1.562 mg/ml against Escherichia coli, Staphylococcus aureus, Klebsiella pneumonia, Pseudomonas aeruginosa and Bacillus subtilis, whereas in aqueous extract was highly sensitive to Bacillus subtilis, E. coli and Klebsiella pneumonia with MIC of 3.125 and 6.25 mg/ml, respectively. Methanolic extracts exerted comparative higher anti-inflammatory activity than aqueous extract.Conclusion: Present study provides a firm evidence to support that the synergistic effect of C. lanatus seed extracts having potent anti-inflammatory and antimicrobial property, which might serve as an effective drug for various microbial infections and inflammatory disorders.

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Comparative Characteristics of Determination of Antioxidant Activity of Sea Buckthorn Fruits by Various Methods

Drug development & registration ◽

10.33380/2305-2066-2019-8-4-48-52 ◽

2019 ◽

Vol 8 (4) ◽

pp. 48-52

Author(s):

O. V. Trineeva

Keyword(s):

Antioxidant Activity ◽

Medicinal Plant ◽

Plant Material ◽

Natural Antioxidants ◽

Sea Buckthorn ◽

In Vitro Tests ◽

Medicinal Plant Material

Introduction. Recently, much attention has been paid to the primary assessment of the pharmacological effect of various drugs using in vivo and in vitro tests. It is known that such a medicinal plant as sea buckthorn, in its phytochemical composition is rich in natural antioxidants: carotenoids, tocopherols, flavonoids, ascorbic acid, etc. In some publications there is information about the antioxidant activity of sea buckthorn and fatty oil based on them. However, information on the comparative characteristics of the use of various methods for determining the antioxidant activity of this type of medicinal plant material and the results obtained are not found in the scientific literature.Aim. The aim of this work was a comparative determination of the antioxidant activity of medicinal plant material of buckthorn fruits of various species of buckthorn.Materials and methods. The total antioxidant activity of water and water-alcohol extracts from the fruits of sea buckthorn fruits was determined using various techniques recommended in the literature. The antioxidant activity of the extracts was determined by permanganometric titration, in vitro inhibition of adrenaline autooxidation, and also in a biological model, Parametium caudatum cell culture.Results and discussion. The effect of the extractant polarity on the value of antioxidant activity was studied. It was found that the highest content of antioxidants in the extraction is observed when using 96 % ethanol as an extractant.Conclusion. Using three methods, the prospects of using sea buckthorn fruits and preparations based on them as a source of antioxidants are shown.

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Anti-Inflammatory Activity of Cnidoscolus aconitifolius (Mill.) Ethyl Acetate Extract on Croton Oil-Induced Mouse Ear Edema

Applied Sciences ◽

10.3390/app11209697 ◽

2021 ◽

Vol 11 (20) ◽

pp. 9697

Author(s):

Eduardo Padilla-Camberos ◽

Omar Ricardo Torres-Gonzalez ◽

Ivan Moises Sanchez-Hernandez ◽

Nestor Emmanuel Diaz-Martinez ◽

Oscar Rene Hernandez-Perez ◽

...

Keyword(s):

Medicinal Plant ◽

Ethyl Acetate ◽

Ethyl Acetate Extract ◽

Inflammatory Activity ◽

Therapeutic Effects ◽

Ear Edema ◽

Croton Oil ◽

Mouse Ear ◽

Anti Inflammatory ◽

Mouse Ear Edema

Nowadays, there is a growing interest in the development of medicinal plant-based therapies to diminish the ravages of the inflammatory process related to diseases and tissue damage. Most therapeutic effects of these traditional medicinal plants are owed to their phenolic and antioxidant properties. C. aconitifolius is a traditional medicinal plant in Mexico. Previous characterization reports have stated its high nutritional and antioxidant components. The present study aimed to better understand the biological activity of C. aconitifolius in inflammation response. We developed an ethyl acetate extract of this plant to evaluate its anti-inflammatory capacity and its flavonoid content. The topical anti-inflammatory effect of the ethyl acetate extract of C. aconitifolius was determined by the croton oil-induced mouse ear edema test, while flavonoid detection and concentration were determined by thin layer chromatography and the aluminum chloride colorimetric assay, respectively. Topical application of the extract showed significant inhibition of the induced-ear edema (23.52 and 49.41% for 25 and 50 mg/kg dose, respectively). The extract also exhibited the presence of flavonoids. The finding of the anti-inflammatory activity exerted by the C. aconitifolius and the identification of its active principles may suggest and support its use for inflammation treatment.

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Anti-Inflammatory Activity and Composition ofSenecio salignusKunth

BioMed Research International ◽

10.1155/2013/814693 ◽

2013 ◽

Vol 2013 ◽

pp. 1-4 ◽

Cited By ~ 4

Author(s):

Cuauhtemoc Pérez González ◽

Roberto Serrano Vega ◽

Marco González-Chávez ◽

Miguel Angel Zavala Sánchez ◽

Salud Pérez Gutiérrez

Keyword(s):

Medicinal Plant ◽

Methanol Extract ◽

Chloroform Extract ◽

Inflammatory Activity ◽

Paw Edema ◽

Active Fraction ◽

Ear Edema ◽

Methanol Extracts ◽

Anti Inflammatory ◽

Inflammatory Effect

We investigated the anti-inflammatory activity ofSenecio salignus. This medicinal plant is often used in Mexico for the treatment of fever and rheumatism. Chloroform and methanol extracts of the plant were tested on 12-O-tetradecanoylphorbol-13-acetate- (TPA-) induced edema in mice ears. The methanol extract of the plant inhibited edema by36±4.4% compared with the control, while the chloroform extract exhibited an even greater level of inhibition (64.1%). The chloroform extract was then fractionated, and the composition of the active fraction was determined by GC-MS. The anti-inflammatory activity of this fraction was then tested on TPA-induced ear edema in mice, and we found that the active fraction could inhibit edema by 46.9%. The anti-inflammatory effect of the fraction was also tested on carrageenan-induced paw edema in rats at doses of 100 mg/kg; a58.9±2.8% reduction of the edema was observed 4 h after administration of carrageenan, and the effect was maintained for 5 h.

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Standardization and anti-inflammatory activity of aqueous extract of Psittacanthus plagiophyllus Eichl. (Loranthaceae)

Journal of Ethnopharmacology ◽

10.1016/j.jep.2017.03.029 ◽

2017 ◽

Vol 202 ◽

pp. 234-240 ◽

Cited By ~ 5

Author(s):

Adrielle Nara Serra Bezerra ◽

Laís Tatiele Massing ◽

Ricardo Bezerra de Oliveira ◽

Rosa Helena Veras Mourão

Keyword(s):

Aqueous Extract ◽

Inflammatory Activity ◽

Anti Inflammatory

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Comperative phyto toxicological and anti inflammatory effects of leaves extracts of holoptelea integrifolia

Bangladesh Journal of Medical Science ◽

10.3329/bjms.v17i2.35873 ◽

2018 ◽

Vol 17 (2) ◽

pp. 212-217

Author(s):

Hina Yasin ◽

Shaukat Khalid ◽

Hina Abrar ◽

Ghazala H Rizwani ◽

Rehana Perveen ◽

...

Keyword(s):

Aqueous Extract ◽

Diclofenac Sodium ◽

Medical Science ◽

Inflammatory Activity ◽

Albino Rats ◽

High Dose ◽

Inhibition Activity ◽

Standard Drug ◽

Etoac Extract ◽

Anti Inflammatory

Objective: Plants play valuable role in the new drug discovery and significantly used to treat different diseases. Nowadays scientists are investigating the therapeutically active phytochemical constituents that are safe and producing lesser side effect in comparative to other standard drugs.Methods: The plant Holoptelea integrifolia is medicinally important and this study was carried out to evaluate the anti inflammatory activity of aqueous extract of the leaves of Holoptelea integrifolia in male albino rats wistar stain treated with acetic acid to induced paw edema.Results: Result indicated the significant anti-inflammatory activity while compared with standard drug (diclofenac sodium).Brine shrimp bioassay (cytotoxicity), phytotoxicity, insecticidal and enzyme inhibition activity was performed in different extracts of the leaves of H. integrifolia. Results of brime shrimp bioassay indicating positive lethality at high dose in BuOH and H2O only. While the results of phytotoxicity in all crude extracts displayed mild phytotoxicity(46.3 µg/ml) in high concentrations (1000 µg/ml) except H2O extract showed no phytotoxicity. Result of insecticidal activity revealed that BuOH extract were found more effective against Rhyzoperthadominica, the EtOH extract expressed major while EtOAC extract showed mild activity against Callosobruchusanalis. Aqueous extract possessed no insecticidal activity.Conclusion: Results of Urease inhibition activity suggested that EtOAC and BuOH extracts of this plant expressed no activity while EtOH and H2O possessed mild inhibiting activity.Bangladesh Journal of Medical Science Vol.17(2) 2018 p.212-217

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Isolation of a New Anti-inflammatory 20, 21, 22, 23, 24, 25, 26, 27-Octanorcucurbitacin-type Triterpene from Ibervillea sonorae

Natural Product Communications ◽

10.1177/1934578x1400900106 ◽

2014 ◽

Vol 9 (1) ◽

pp. 1934578X1400900 ◽

Cited By ~ 1

Author(s):

Angel Jardón-Delgado ◽

Gil Alfonso Magos-Guerrero ◽

Mariano Martínez-Vázquez

Keyword(s):

Medicinal Plant ◽

Inflammatory Activity ◽

Anti Inflammatory

A new cucurbitane-type triterpene, 20, 21, 22, 23, 24, 25, 26, 27-octanorcucurbita-5-ene-3, 11, 16-trione (1), named kinoin D, was isolated from the roots of the medicinal plant Ibervillea sonorae, (wereque). The structure of 1 was established on the basis of extensive NMR and MS studies. In addition, the known kinoins B (3) and C (5) were isolated, as were 16α-20,25-trihydroxy-3α-(2- O-α-L-rhamnopyranosiyl-D-glucopyranosyloxy)-(10α)-cucurbit-5-en-11,22-dione (6), (22 S)-16α,22-diacetoxy-20,25-dihydroxy-3α-[3,4,6-tri- O-acetyl-2- O-(2,3,4-tri- O-acetyl-α-L-rhamnopyranosyl)-β-glucopyranosyl]-(10α)-cucurbita-5,23t-dien-11-one (7) and 16α-acetoxy-20,25-dihydroxy-3α-[3,4,6-tri- O-acetyl-2- O-(2,3,4,-tri- O-acetyl-α-L-rhamnopyranosyl)-β-D-glucopyranosyl]-(10α)-cucurbita-5-ene-11,22-dione (8). Compound 1 exhibited anti-inflammatory activity in TPA-induced edema in mice.

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