scholarly journals Comperative phyto toxicological and anti inflammatory effects of leaves extracts of holoptelea integrifolia

2018 ◽  
Vol 17 (2) ◽  
pp. 212-217
Author(s):  
Hina Yasin ◽  
Shaukat Khalid ◽  
Hina Abrar ◽  
Ghazala H Rizwani ◽  
Rehana Perveen ◽  
...  
Keyword(s):  
Aqueous Extract ◽  
Diclofenac Sodium ◽  
Medical Science ◽  
Inflammatory Activity ◽  
Albino Rats ◽  
High Dose ◽  
Inhibition Activity ◽  
Standard Drug ◽  
Etoac Extract ◽  
Anti Inflammatory

Objective: Plants play valuable role in the new drug discovery and significantly used to treat different diseases. Nowadays scientists are investigating the therapeutically active phytochemical constituents that are safe and producing lesser side effect in comparative to other standard drugs.Methods: The plant Holoptelea integrifolia is medicinally important and this study was carried out to evaluate the anti inflammatory activity of aqueous extract of the leaves of Holoptelea integrifolia in male albino rats wistar stain treated with acetic acid to induced paw edema.Results: Result indicated the significant anti-inflammatory activity while compared with standard drug (diclofenac sodium).Brine shrimp bioassay (cytotoxicity), phytotoxicity, insecticidal and enzyme inhibition activity was performed in different extracts of the leaves of H. integrifolia. Results of brime shrimp bioassay indicating positive lethality at high dose in BuOH and H2O only. While the results of phytotoxicity in all crude extracts displayed mild phytotoxicity(46.3 µg/ml) in high concentrations (1000 µg/ml) except H2O extract showed no phytotoxicity. Result of insecticidal activity revealed that BuOH extract were found more effective against Rhyzoperthadominica, the EtOH extract expressed major while EtOAC extract showed mild activity against Callosobruchusanalis. Aqueous extract possessed no insecticidal activity.Conclusion: Results of Urease inhibition activity suggested that EtOAC and BuOH extracts of this plant expressed no activity while EtOH and H2O possessed mild inhibiting activity.Bangladesh Journal of Medical Science Vol.17(2) 2018 p.212-217

scholarly journals Anti-Inflammatory Activity of Hydroxytriazenes and their Vanadium Complexes

2008 ◽  
Vol 5 (s2) ◽  
pp. 1144-1148 ◽  
Author(s):  
Kalpana Singh ◽  
Peeyush Patel ◽  
A. K. Goswami
Keyword(s):  
Body Weight ◽  
Metal Complexes ◽  
Diclofenac Sodium ◽  
Inflammatory Activity ◽  
Albino Rats ◽  
Vanadium Complexes ◽  
Standard Drug ◽  
Standard Methods ◽  
Paw Oedema ◽  
Anti Inflammatory

The anti-inflammatory activity of hydroxytriazenes as well as their vanadium complexes has been studied using carrageenan induced hind paw oedema method in albino rats (Wister strain). Hydroxytriazenesviz., 3-hydroxy-1,3-diphenyltriazene(HT-1), 3-hydroxy-3-phenyl-1-(4-sulfonamido)phenyl triazene(HT-2), 3-hydroxy-3-p-chlorophenyl-1-(4-sulfonamido) phenyltriazene(HT-3), 3-hydroxy-3-m-chlorophenyl-1-(4-sulfonamido)phenyltriazene(HT-4)(HT-4) and their respective vanadium complexes C-1, C-2, C-3 and C-4 have been synthesized using standard methods, purified, characterized and used for studying their anti-inflammatory activities. The hind paw oedema was produced by subplanter injection of carrageenan and the paw volume was measured plethysmographically after 0.5, 1, 2, 3 and 5 h. The animals were givenHT-1, HT-2, HT-3andHT-4and also vanadium complexes C-1, C-2, C-3 and C-4 dissolved inDMSO(at dose 5 mg/Kg body weight). Diclofenac sodium (5 mg/kg) was used as a standard drug. The standard drug shows maximum inibition up to 1h as 81.73% which goes on increasing up to 3h (88.94%) but further reduces to 74.93% at the end of 5 h. The test compounds i.e. both ligands as well as their metal complexes show maximum percent inhibition only up to 1h. after which the efficacy reduces. Thus it can be said that both ligands as well as their vanadium complexes show very significant anti-inflammatory activity up to 1h which is comparable to standard drug.

scholarly journals EVALUATION OF EFFECT OF AQUEOUS EXTRACT OF PORTULACA OLERACEA LINN. LEAVES IN ACUTE INFLAMMATION

2020 ◽  
pp. 1-2
Author(s):  
M. Medhabati ◽  
L. Babycha ◽  
L. Tarinita ◽  
Bikram Tewari ◽  
Saumya Kanti Sinha
Keyword(s):  
Aqueous Extract ◽  
Acute Inflammation ◽  
Portulaca Oleracea ◽  
Inflammatory Activity ◽  
Albino Rats ◽  
Standard Drug ◽  
Paw Oedema ◽  
The Mean ◽  
Anti Inflammatory ◽  
Inflammatory Action

In the present study, the anti-inflammatory activity of aqueous extract of Portulaca oleracea (POE 200, 400 and 600mg/kg) was studied using carrageenan induced paw oedema in albino rats. The mean increase in paw volume was recorded. POE produced significant (p<,0.01) anti-inflammatory activity when compared to the control. The anti-inflammatory action of POEcan be attributed to its flavonoid contents which are known to act through inhibition of prostaglandin biosynthesis. However, the test drug at maximum dose (600mg/kg) was found to be less effective than the standard drug, aspirin (100mg/Kg).

scholarly journals EVALUATION OF THE ANTI-INFLAMMATORY ACTIVITY OF COMBINATION OF ETHANOL EXTRACTS OF AZADIRACHTA INDICA (NEEM) AND LAWSONIA INERMIS (HENNA)

2016 ◽  
Vol 9 (5) ◽  
pp. 256
Author(s):  
Tirupathi Rao ◽  
Renuka P ◽  
Akhil P ◽  
Divya P ◽  
Devi Priyanka P
Keyword(s):  
Azadirachta Indica ◽  
Protein Denaturation ◽  
Diclofenac Sodium ◽  
Inflammatory Activity ◽  
Cell Protein ◽  
Inhibition Activity ◽  
Lawsonia Inermis ◽  
Standard Drug ◽  
Anti Inflammatory

ABSTRACTObjective: The aim of the present study was to investigate the in vitro anti-inflammatory activity of Azadirachta indica (neem) and Lawsonia inermis(henna) individual extract and in combination using the same solvent.Methods: The leaf material of A. indica and L. inermis was collected from surroundings of Aditya College of Pharmacy, Kakinada, East Godavari.Powdered material was subjected to successive solvent extraction process. The yield was collected and prepared different concentrations (50, 100,and 200 µg/ml) of plant extracts. Diclofenac sodium was used as standard drug. The anti-inflammatory activity was performed by in vitro methodssuch as albumin denaturation method and human red blood cells membrane lysis method.Results: Denaturation of proteins is a well-documented cause of inflammation. Neem showed a significant membrane stabilizing activity of 46.62%and protein denaturation inhibition activity of 57.32% at concentration of 200 µg/ml. Henna showed a significant membrane stabilizing activityof 39.89% and protein denaturation inhibition activity of 53.75% at 200 µg/ml. In combination, both the extracts showed a significant membranestabilizing activity of 56.63% and protein denaturation inhibition activity of 67.69% at concentration of 200 µg/ml.Conclusion: The present study concluded that combination of A. indica and L. inermis possesses significant anti-inflammatory activity when comparedwith individual extract.Keywords: Anti-inflammatory, Human red blood cell, Protein denaturation, Lawsonia inermis, Azadiracta indica.

scholarly journals Evaluation of anti - inflammatory activity of aqueous extract of leaves of hibiscus sabdariffa in albino rats

2017 ◽  
Vol 6 (5) ◽  
pp. 1155
Author(s):  
Soumya B. Patil ◽  
Swetha Munoli
Keyword(s):  
Aqueous Extract ◽  
Inflammatory Activity ◽  
Albino Rats ◽  
Paw Edema ◽  
Standard Drug ◽  
Inflammatory Agent ◽  
Chronic Model ◽  
Inflammatory Drugs ◽  
Anti Inflammatory ◽  
Acute And Chronic Inflammation

Background: Inflammation continues to be an area of great interest for research, probably due to the non-availability of a safer and more effective analgesic and anti-inflammatory agent. This has led to increase in demand for natural products with anti-inflammatory activity having fewer side effects. Non-steroidal anti-inflammatory drugs (NSAIDs) such as indomethacin are used in the treatment of inflammation, fever and pain. However, NSAIDs cause gastric damage as a major adverse reaction. In this study, the anti-inflammatory activity of aqueous extract H. sabdariffa Linn was studied using carrageenan and formalin for acute and chronic inflammation respectively in albino rats.Methods: In this study, anti-inflammatory activity of H. sabdariffa was studied and compared with standard drug, indomethacin. Aqueous extract of leaves of H. sabdariffa (200mg/kg or 400mg/kg) and indomethacin (25mg/kg) were used to separate groups of rats and paw edema was measured by plethysmometer and compared with control group.Results: H. sabdariffa did not show any significant reduction of paw edema (P >0.05) in both the models of inflammation i.e. carrageenan induced acute model and formalin induced chronic model of inflammation.Conclusions: H. sabdariffa didn’t show any significant anti-inflammatory activity.

A Facile One-Pot Synthesis of 3-Methylbenzisoxazoles via a Key Intermediate of ortho-Ethoxyvinyl Nitroaryls by Domino Rearrangement and Their Anti- Inflammatory Activity

2020 ◽  
Vol 16 (8) ◽  
pp. 1161-1165
Author(s):  
Bashetti Nagaraju ◽  
Jagarlapudi V. Shanmukhakumar ◽  
Nareshvarma Seelam ◽  
Tondepu Subbaiah ◽  
Bethanamudi Prasanna
Keyword(s):  
Stannous Chloride ◽  
Biologically Active ◽  
Inflammatory Activity ◽  
Albino Rats ◽  
One Pot ◽  
Simple Method ◽  
Standard Drug ◽  
Entire Study ◽  
One Pot Synthesis ◽  
Anti Inflammatory

Background: Recently, there has been a lot of scientific interest in exploring the syntheses of oxygen and nitrogen-containing heterocyclic compounds due to their pharmacological activities. In addition, benzisoxazoles play a very important role in organic synthesis as key intermediates. Objective: In this paper, we focused on developing a novel synthetic route for biologically active arylisoxazoles under normal conditions, and simplified it to get high purities and yields, and also reported their anti-inflammatory activities. Method: An efficient and simple method has been explored for the synthesis of novel 3-methyl arylisoxazoles from o-nitroaryl halides via o-ethoxyvinylnitroaryls, using dihydrated stannous chloride (SnCl2.2H2O) in MeOH / EtOAc (1:1) via Domino rearrangement in one pot synthesis. Result: We synthesized novel 3-methylarylisoxazoles from o-nitroarylhalides via o-ethoxyvinylnitroaryls, using dihydrated stannous chloride (SnCl2.2H2O) in MeOH / EtOAc (1:1) via domino rearrangement. In this reduction, nitro group and ethoxy vinyl group change to the functional acyl ketones, followed by hetero cyclization. Here, the reaction proceeds without the isolation of intermediates like 2-acylnitroarenes and 2- acylanilines. All the synthesized compounds were completely characterized by the NMR and mass spectra. The compounds were also explored for their anti-inflammatory activity by carrageenan-induced inflammation in the albino rats (150-200 g) of either sex used in this entire study with the use of Diclofenac sodium as the standard drug. The initial evaluations identified leading targets with good to moderate anti-inflammatory activity. Conclusion: A simple, one-pot and convenient method has been explored for the synthesis of novel 3- methylarylisoxazoles with high purity and reaction yields. All the compounds 3a, 3c, 3d, 3f, 3g and 3h exhibited 51-64% anti-inflammatory activities.

Analgesic and anti-inflammatory activity of Tectona grandis Linn. stem extract

2015 ◽  
Vol 26 (5) ◽  
Author(s):  
Sapna P. Giri ◽  
Sushilkumar B. Varma
Keyword(s):  
Analgesic Activity ◽  
Radiant Heat ◽  
Inflammatory Activity ◽  
Albino Rats ◽  
Standard Drug ◽  
Writhing Test ◽  
Paw Oedema ◽  
Stem Extract ◽  
Heat Method ◽  
Anti Inflammatory

AbstractThe objective of this work was to study the analgesic and anti-inflammatory activity ofHealthy albino rats (150–200 g) and mice (25–50 g) were arranged and divided into five groups. Group 1 received distilled water and served as control, groups 2, 3, 4 were treated with TG stem extract (100, 200, 400 mg/kg) and group 5 received standard drug. For analgesic activity, the radiant heat method and writhing test were used, and for anti-inflammatory activity, the paw oedema model and granuloma pouch method were used. Statistical analysis was done by one-way analysis of variance followed by the t-test.In the radiant heat method, there was a dose-dependent increase in reaction time in TG stem extract (100, 200, 400 mg/kg) and standard drug (buprenorphine). In the writhing test, TG stem extract (200, 400 mg/kg) and aspirin decreased the number of writhes which was significant (p<0.01, p<0.001) as compared to controls. The percentage protection was 8.88%, 15.55%, 22.22% and 35.55% in groups 2, 3, 4 and 5, respectively. In the rat paw oedema test, TG stem extract (200, 400 mg/kg) and aspirin showed 20%, 40% and 54% inhibition of oedema which was statistically significant (p<0.05, p<0.01, p<0.001), respectively, as compared to control at the end of 4 h. In the granuloma pouch method, TG stem extract (100, 200, 400 mg/kg) and aspirin showed 13.33%, 22.22%, 33.33% and 66.66% inhibition of granuloma, respectively, which was significant (p<0.05, p<0.01, p<0.001).TG stem extract possesses significant anti-inflammatory and analgesic activity.

scholarly journals Synthesis, characterization and biological evaluation of some novel N-Mannich bases of heterocyclic 1,3,4-thiadiazole.

2019 ◽  
Vol 9 (4-A) ◽  
pp. 220-228
Author(s):  
Piush Sharma ◽  
Charanjeet Singh
Keyword(s):  
Mannich Reaction ◽  
Mannich Bases ◽  
Biological Evaluation ◽  
Inflammatory Activity ◽  
Albino Rats ◽  
Plate Method ◽  
Standard Drug ◽  
Excellent Activity ◽  
Anti Inflammatory

A series of some novel N-Mannich bases of heterocyclic 1,3,4-thiadiazole were synthesized through the condensation reaction of 1,3,4-thiadiazole containing a aromatic secondary amine, aromatic aldehydes and cyclic compounds employing Mannich reaction and using conventional synthesis. All the synthesized compounds were obtained in the range of 57.41-83.3 % yield. The structures of synthesized compounds were confirmed by UV, IR, 1H NMR spectroscopy. the essential structural features responsible for interaction with receptor site are established within a suggested pharmacophore. The in vitro antibacterial activity of the synthesized compounds was determined, against two Gram-positive bacteria, viz. S. aureus & B. subtilis and Gram-negative, viz. E. coli and K. pneumoniae, by cup-plate method using the standard drug ciprofloxacin. Minimum inhibitory concentrations (MIC) changed in the range of 1.56_ _ 200 mg mL_1. Compound 3b exhibited excellent activity against both bacteria. The in vitro antifungal activity of the synthesized compound was also evaluated by cup-plate method against the fungi A. niger and C. albicans compared with the standard drug Fluconazole. Compound 4a, 8a exhibited excellent activity against both fungi. The result has shown that the compounds are quite active against pathogens under study and were nontoxic. The anti-inflammatory activity of the compound was evaluated, on albino rats, by carageenan induced rat paw oedema method using the standard drug diclofenac sodium. Compound 7b and 8c exhibited excellent anti-inflammatory and analgesic pharmacological activities. Structurally the compound 7b has a greater number of unsaturated hydrocarbons in schiff base, which shows good lipophilic properties within electron rich morpholine ring in Mannich base. Statistical significance of differences between group was determined by one-way analysis of variance (ANOVA). Among the synthesized compounds 3a, 4b, 5c, 7b, 8a and 8c were found be the most active. All the synthesized compounds were found to be low lethal as ascertained by LD50 test. Keywords: N- Mannich bases of heterocyclic 1,3,4-thiadiazole derivatives; Mannich reaction; antimicrobial agents; anti-inflammatory activity;

SYNTHESIS, DOCKING STUDY AND ANTI-INFLAMMATORY STUDIES OF SOME FLAVANONES

INDIAN DRUGS ◽  
2021 ◽  
Vol 58 (02) ◽  
pp. 54-60
Author(s):  
Ishwar K. Bhat ◽  
Keyword(s):  
Diclofenac Sodium ◽  
Docking Study ◽  
Inflammatory Activity ◽  
Mass Spectral Data ◽  
Ligand Interaction ◽  
Standard Drug ◽  
Mass Spectral ◽  
H Nmr ◽  
Anti Inflammatory ◽  
Phosphoinositide 3 Kinase

In this work, a series of flavanones (P1-P9) was synthesized by cyclization of substituted (hydroxyphenyl)- 3-(phenyl) prop-2-en-1-ones (S1-S9). The structures of the synthesized compounds were characterized by IR, 1 H NMR and mass spectral data. These derivatives were evaluated for anti-inflammatory activity. Compounds P1, P3, P6 and P7 showed excellent anti-inflammatory activity as compared to standard drug diclofenac sodium. Molecular docking of these flavanones (P1-P9) was also performed with receptor phosphoinositide-3-kinase PI3Kδ (PDB code: 5ITD). All the flavanones (P1-P9) were docked into same groove of the binding site of native co-crystallized (5-{4-[3-(4-acetylpiperazine-1-carbonyl) phenyl] quinazolin-6-yl}-2-methoxypyridine carbonitrile) ligand for activity explanation and exhibited good ligand interaction and binding affinity were of range -4.57 to -8.79kcal/mol.

scholarly journals SYNTHESIS AND EVALUATION OF ANTI-INFLAMMATORY AND ANTIBACTERIAL ACTIVITIES OF SOME 1, 2-BENZISOXAZOLE DERIVATIVES

2016 ◽  
Vol 8 (4) ◽  
pp. 64 ◽  
Author(s):  
Sarath Sasikumar ◽  
Haripriya M. ◽  
Anjali T.
Keyword(s):  
Antibacterial Activity ◽  
Diclofenac Sodium ◽  
Analytical Techniques ◽  
Antibacterial Activities ◽  
Inflammatory Activity ◽  
Antibacterial Drug ◽  
Standard Drug ◽  
Good Antibacterial Activity ◽  
Anti Inflammatory ◽  
And Staphylococcus Aureus

Objective: A series of 1, 2-Benzisoxazole derivatives were synthesized and characterized by various analytical techniques like Melting point, Rf, FTIR, and NMR spectra.Methods: Structures of the compounds were elucidated and evaluated for anti-inflammatory activity by HRBC membrane stabilization method, antibacterial activity against Escherichia coli and Staphylococcus aureus by cup plate method.Results: The compounds 4a and 4e showed good anti-inflammatory activity compared with standard drug Diclofenac sodium and compounds 4b and 4d showed good antibacterial activity on compared with standard drug Gentamycin.Conclusion: These compounds may serve as future leads for anti-inflammatory and antibacterial drug discovery.

scholarly journals PRELIMINARY PHYTOCHEMICAL SCREENING AND ANTI-INFLAMMATORY EFFECT OF THE AQUEOUS EXTRACT OF TABERNAEMONTANA DIVARICATA FLOWER IN WISTER RATS

2017 ◽  
pp. 9-12
Author(s):  
Deepankar Garga ◽  
Trishna Das
Keyword(s):  
Aqueous Extract ◽  
Inflammatory Activity ◽  
Phytochemical Analysis ◽  
Standard Drug ◽  
Tabernaemontana Divaricata ◽  
Rat Paw Edema ◽  
Oil Extract ◽  
Anti Inflammatory ◽  
Rat Paw ◽  
Inflammatory Effect

Objective: To determine the phytochemical analysis and anti-inflammatory effect of the aqueous extract of Tabernaemontanadivaricata flower in Wister Rats.Methods: The aqueous extract of fresh flowers of the plant Tabernaemontanadivaricata (family-Apocyanaceae) were prepared and analyzed for phytochemical constituents using standard methods. The anti-inflammatory activity of the plant extracts was examined with the help of rat models by taking diclofenac as a standard drug against T. divericata oil extract by using carrageenan induced rat paw edema method with the help of the instrument plethesmometer.Results: The present experiment shows the phytochemical analysis, anti-inflammatory activity of the aqueous extract of fresh flowers of the plant Tabernaemontanadivaricata (family-Apocyanaceae). Various phytochemical analysis revealed the presence of saponins, flavonoids, glycosides, steroids, proteins, phenols, amino acids and tannins. The oil extract of the plant showed much effective anti-inflammatory activity against the standard drug.Conclusion: It can be concluded aqueous extract of the flower of the plant Tabernaemontanadivaricata (family-Apocyanaceae) contain the high presence of phytochemicals. This extract was found to possess promising antimicrobial activity when compared with the standards.

Sign in / Sign up

Export Citation Format

Share Document