Syntrophic co-culture of Bacillus subtilis and Klebsiella pneumonia for degradation of kraft lignin discharged from rayon grade pulp industry

2015 ◽  
Vol 33 ◽  
pp. 229-238 ◽  
Author(s):  
Sangeeta Yadav ◽  
Ram Chandra
Keyword(s):  
Bacillus Subtilis ◽  
Kraft Lignin ◽  
Klebsiella Pneumonia ◽  
Pulp Industry

scholarly journals EVALUATION OF IN VITRO ANTI-INFLAMMATORY AND ANTIMICROBIAL ACTIVITY OF AQUEOUS AND METHANOLIC SEED EXTRACTS OF CITRULLUS LANATUS

2017 ◽  
Vol 9 (5) ◽  
pp. 29
Author(s):  
Iswariya S. ◽  
Uma T. S.
Keyword(s):  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Aqueous Extract ◽  
Seed Extract ◽  
Inflammatory Activity ◽  
Diffusion Method ◽  
Klebsiella Pneumonia ◽  
Anti Inflammatory ◽  
Seed Extracts

Objective: The present study was designed to identify the bioactive phytochemicals and its antibacterial and in vitro anti-inflammatory potential of aqueous and methanolic seed extract of Citrullus lanatus.Methods: The phytochemical screening of both the aqueous and methanolic seed extract was carried out qualitatively to identify the major Phyto-constituents present in the extracts. The antimicrobial activity of the extracts was evaluated against six pathogenic bacterial strains by agar well diffusion method and the Minimum inhibitory concentration (MIC) was determined by broth dilution method. In vitro anti-inflammatory activity of C. lanatus seed extracts was evaluated by using human red blood cell (HRBC) membrane stabilization and inhibition of albumin denaturation method.Results: The results of the study indicated that both the extracts of the seed having antimicrobial activity, while the methanolic extract showed more significant activity against the tested organism than aqueous extract. Methanol extract had the lowest MIC of 1.562 mg/ml against Escherichia coli, Staphylococcus aureus, Klebsiella pneumonia, Pseudomonas aeruginosa and Bacillus subtilis, whereas in aqueous extract was highly sensitive to Bacillus subtilis, E. coli and Klebsiella pneumonia with MIC of 3.125 and 6.25 mg/ml, respectively. Methanolic extracts exerted comparative higher anti-inflammatory activity than aqueous extract.Conclusion: Present study provides a firm evidence to support that the synergistic effect of C. lanatus seed extracts having potent anti-inflammatory and antimicrobial property, which might serve as an effective drug for various microbial infections and inflammatory disorders.

Production of (2S,3S)-2,3-butanediol and (3S)-acetoin from glucose using resting cells of Klebsiella pneumonia and Bacillus subtilis

2011 ◽  
Vol 102 (22) ◽  
pp. 10741-10744 ◽  
Author(s):  
Zhen Liu ◽  
Jiayang Qin ◽  
Chao Gao ◽  
Dongliang Hua ◽  
Cuiqing Ma ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Klebsiella Pneumonia ◽  
Resting Cells

scholarly journals Synthesis, Characterization and Bioactivity Profiling of Gold Nanoparticles of Trachyspermum Ammi Crude Extract

2021 ◽  
Author(s):  
Saud Bawazeer ◽  
Abdur Rauf ◽  
Humaira Naz ◽  
Syed Uzair Ali Shah ◽  
Dania Saad Waggas ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Gold Nanoparticles ◽  
Bacillus Subtilis ◽  
Crude Extract ◽  
Broad Band ◽  
Soluble Starch ◽  
Klebsiella Pneumonia ◽  
Trachyspermum Ammi ◽  
Uv Visible

Trachyspermum ammi seeds were selected for photochemical study. The crude T. ammi methanol and aqueous extracts showed the presence of alkaloids, saponins, steroids, terpenoids, coumarins, betacyanins, flavonoids and soluble starch. The synthesis of gold nanoparticles (AuNPs) using T. ammi extract was characterized using UV-visible, and FT-IR spectroscopy. The appearance of sharp peak at 520 nm in the UV visible spectra, and the appearance of broad band nanoparticles spectra at 563.21 cm-1, 516.92 cm-1 and 462.92 cm-1 as well as the disappearance of the carboxyl OH bond and carbon-carbon triple bond supported the formation of AuNPs. The crude T. ammi methanol and AuNPs were investigated for antioxidant potential using DPPH· free radical assay, which shows that crude extract has significant antioxidant effect. The synthesized AuNPs was also evaluated for antibacterial activities against staphylococcus aureus, Klebsiella pneumonia and Bacillus subtilis. The crude extract showed activity against Bacillus subtilis, while AuNPs showed activity against staphylococcus aureus. The in vivo sedative effect and analgesic effect were enhanced in AuNPs treated animals in 5 times less dose (i.e., 5, and 10 mg/kg) than that of crude extract. It was concluded that T. ammi extract include capping and reducing agents, which make it capable to be developed as stable AuNPs. The biological action of AuNPs is either enhanced (sedative and analgesic) or changed (antibacterial activity), when compared with that of plant extract.

scholarly journals POLA BAKTERI YANG BERPOTENSI MENJADI SUMBER PENULARAN INFEKSI NOSOKOMIAL DI IRINA C RUANGAN INTERMEDIATE CARE (IMC) BLU RSUP PROF. DR. R. D. KANDOU MANADO

2015 ◽  
Vol 3 (1) ◽  
Author(s):  
Claudya Emma Sulistya ◽  
Olivia Waworuntu ◽  
John Porotu’o
Keyword(s):  
Bacillus Subtilis ◽  
Nosocomial Infection ◽  
Enterobacter Aerogenes ◽  
Klebsiella Pneumonia ◽  
Intermediate Care ◽  
Enterobacter Agglomerans ◽  
Morganella Morganii ◽  
Care Workers ◽  
Identification Of Bacteria ◽  
Very High

Abstract: The hospital is a place to care for patients with various diseases such as infectious diseases, ranging from mild to severe. It can cause the infection spread from one patient to another, as well as with health care workers who are often exposed to infectious agents. Hospitalized patients at very high risk for nosocomial infection, caused by the condition of their disease, and the risk is compounded when patients undergoing invasive procedures. This is caused non-pathogenic microorganisms capable of causing disease. Research or clinical testing in the laboratory necessary to determine whether the treatment in IMC has the potential to become source of nosocomial infection transmission. The purpose of this study was to determine the source and microorganisms that could potentially lead to the transmission of nosocomial infections in IRINA C Intermediate Care (IMC) BLU Dr Prof. Dr R. D. Kandou. This study is a prospective descriptive study. The samples are floor and wall of treatment rooms, indoor furniture, medical equipment and air in IRINA C Intermediate Care (IMC) BLU Dr Prof. Dr RD Kandou Manado. There are 30 samples and swabs taken. Identification of bacteria were performed with culture medium. In this study, Bacillus subtilis found in 10 samples (33.4%), Enterobacter agglomerans in 4 samples (13.4%), Staphylococcus in 4 samples (13.4%), Klebsiella pneumonia in 3 samples (10%), Coccus grams negative in 2 samples (6.7%), Candida in 2 samples (6.7%), Morganella Morganii in 1 sample (3.4%), Enterobacter aerogenes in 2 samples (6.7%), Streptococcus in 1 sample (3.4%), and Salmonella in 1 sample (3.4%). This research concluded that Bacillus subtilis is the most found bacteria .Keywords: Nosocomial infection, IMC, bacteria.Abstrak: Rumah sakit merupakan tempat pelayanan pasien dengan berbagai macam penyakit yang diantaranya penyakit karena infeksi, mulai dari ringan sampai berat. Hal ini dapat menyebabkan resiko penyebaran infeksi dari satu pasien ke pasien lainnya, begitupun dengan petugas kesehatan yang sering terpapar dengan agen infeksi. Pasien rawat inap beresiko sangat tinggi untuk terjadinya infeksi nosokomial, ini disebabkan karena kondisi penyakit mereka, dan risiko diperparah ketika pasien menjalani prosedur invasif. Hal ini menyebabkan mikroorganisme yang biasanya tidak patogen mampu menyebabkan penyakit. Diperlukan penelitian atau pengujian secara klinis di laboratorium untuk mengetahui apakah di ruang perawatan IMC mempunyai potensi sumber penularan infeksi nosokomial. Tujuan dari penelitian ini adalah untuk mengetahui sumber dan mikroorganisme yang berpotensi menyebabkan penularan infeksi nosokomial di IRINA C Intermediate Care (IMC) BLU RSUP Prof. Dr. R. D. Kandou. Penelitian ini menggunakan metode penelitian deskriptif prospektif. Sampel yang diteliti adalah lantai dan dinding ruangan perawatan, perabotan ruangan, peralatan medis dan udara di IRINA C Intermediate Care (IMC) BLU RSUP Prof. Dr. R. D. Kandou Manado sebanyak 30 sampel dan diambil secara usapan. Identifikasi bakteri dilakukan dengan media kultur. Pada hasil penelitian menunjukan Bacillus subtilis 10 sampel (33,4%), Enterobacter agglomerans sebanyak 4 sampel (13,4%), Staphylococcus sebanyak 4 sampel (13,4%), Klebsiella Pneumonia sebanyak 3 sampel (10%), Coccus gram negatif sebanyak 2 sampel (6,7%), Kandida sebanyak 2 sampel (6,7%), Morganella Morganii sebanyak 1 sampel (3,4%), Enterobacter aerogenes sebanyak 2 sampel (6,7%), Streptococcus sebanyak 1 sampel ( 3,4%), dan Salmonella sebanyak 1 sampel (3,4%). Kesimpulan pada penelitian ini jenis bakteri terbanyak ditemukan adalah Bacillus subtilis.Kata kunci: Infeksi nosokomial, IMC, bakteri.

scholarly journals In vitro Antimicrobial Potential of Samples From the Leaves and Fruits of Medicinally Important Melia azedarach

2021 ◽  
Vol 64 (2) ◽  
pp. 109-115
Author(s):  
Jehan Bakht ◽  
Ayesha Bukhari ◽  
Arshad Iqbal ◽  
Mohammad Shafi
Keyword(s):  
Escherichia Coli ◽  
Bacillus Subtilis ◽  
Antimicrobial Activities ◽  
Melia Azedarach ◽  
Klebsiella Pneumonia ◽  
Gram Negative ◽  
Antibacterial And Antifungal Activities ◽  
Antibacterial And Antifungal ◽  
Micro Organisms

Different solvent extracted samples obtained from the leaves and fruits of Melia azedarach were investigated for their antibacterial and antifungal activities. All the extracts from both tissues sources showed varying degrees of antimicrobial activities. Ethyl acetate and n-butanol extracts of both tissues inhibited the growth of the tested micro-organisms at all concentrations. N-hexane extracted fractions of the leaves reduced the activity of Bacillus subtilis, Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa at all concentrations, while no growth inhibition was recorded against Klebsiella pneumonia and Candida albicans. Crude methanolic extract from the leaves tissues showed activity against all the tested microbial species and highest susceptibility was noted against Bacillus subtilis. In case of leaves, the most susceptible bacteria were Bacillus subtilis (gram positive) and Klebsiella pneumonia (gram negative) was the most resistant one. In case of fruits extracts, the most susceptible bacteria were Klebsiella pneumonia (gram negative) and Escherichia coli (gram negative) was the most resistant one.  

scholarly journals Anti microbial activity of different dosage forms of Bakuchi (Psoralea corylifolia Linn.) taila, An Ayurvedic formulation

2015 ◽  
Vol 6 (3) ◽  
Author(s):  
Acharya M J ◽  
Singh T R ◽  
Patgiri B J
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Bacillus Subtilis ◽  
Traditional Medicine ◽  
Microbial Activity ◽  
Skin Disease ◽  
Dosage Forms ◽  
Klebsiella Pneumonia ◽  
Psoralea Corylifolia ◽  
Gram Negative

           Psoralea corylifolia Linn. commonly known as “Bakuchi” used in Indian traditional medicine. Ayurveda for the treatment of leucoderma, scabies, leprosy, psoriasis, dermatitis etc. Bakuchi taila is one of the Ayurvedic formulation which is used externally in skin disease. Hence, an attempt has been made to convert Bakuchi taila into its different dosage forms and to evaluate anti microbial activity against gram positive and gram negative bacilli. Bakuchi taila, Bakuchi gel, Bakuchi siktha taila and Bakuchi ointment possess significant anti microbial activity against Bacillus subtilis, Staphylococcus aureus, Escherichia coli and Klebsiella pneumonia. These results confirmed that potential of Bakuchi seeds (Psoralea corylifolia Linn.) in the development of Ayurvedic topical skin formulations. 

Isolation and Characterization of Hydrocarbon-degrading Bacteria in Soils of Mechanical Workshops in Maiduguri, Borno State

2021 ◽  
Vol 4 (2) ◽  
pp. 35-38
Author(s):  
Hussaini Shettima ◽  
Ibrahim Alkali Allamin ◽  
Nasir Halima ◽  
Haruna Yahya Ismail ◽  
Yusuf Musa
Keyword(s):  
Bacillus Subtilis ◽  
Bacillus Licheniformis ◽  
Heterotrophic Bacteria ◽  
Bacterial Species ◽  
Bacillus Coagulans ◽  
Engine Oil ◽  
Klebsiella Pneumonia ◽  
Isolation And Characterization ◽  
Degrading Bacteria ◽  
Hydrocarbon Utilizing Bacteria

The study was conducted to determine the distribution of hydrocarbon utilizing bacteria in spent engine oil (SEO) contaminated soil. Five mechanical workshops within Maiduguri Metropolis. Five bulk soil sample comprising of one each of the five sites; the sites are Leventis Area on Kashim Ibrahim Way, Damboa Raod, Boiler (commonly called Bola) near Maiduguri Monday Market, Ngomari, on Kano Road, and 1000 Housing Estate, also on Kano Road. Nutrient agar was used, or isolation and enumeration total heterotrophic bacteria and Mineral salt agar was used for the isolation and enumeration of hydrocarbon utilizing bacteria. The result shows THB (92.0x104) at Damboa road (DR) to as higher and (78.8x104) at Bola area (BL) while HUB (2.0x104) at Damboa road (DR) and as high as (9.3x104) at Bola area (BL). The bacterial species isolated were species of Bacillus licheniformis, Bacillus subtilis, Bacillus coagulans, Bacillus alvei, Bacillus cereus, Bacillus lentus other are Pseudomonas aeruginosa, Klebsiella pneumonia. Bacillus licheniformis, and Bacillus subtilis are the most occurring bacterial isolates identified. The ability of those bacterial isolate to degrade hydrocarbon buoyantly will help in remediation of oil polluted environments.

scholarly journals Biorefinery review: Wide-reaching products through kraft lignin

BioResources ◽  
2019 ◽  
Vol 14 (3) ◽  
pp. 7543-7581 ◽  
Author(s):  
Iara F. Demuner ◽  
Jorge Luiz Colodette ◽  
Antonio J. Demuner ◽  
Carolina M. Jardim
Keyword(s):  
Large Scale ◽  
Paper Industry ◽  
Complex Structure ◽  
Value Added ◽  
Chemical Conversion ◽  
Kraft Lignin ◽  
Transportation Fuels ◽  
Pulp Industry ◽  
Sulfite Pulp ◽  
Process Energy

This review details the structure of lignin and curates information on the characteristics that this polymer must have for each specific use. Lignin is a by-product of the pulp and paper industry and the second most abundant biopolymer after cellulose. Approximately 50 million tons of lignin are produced worldwide annually, of which 98% to 99% is incinerated to produce steam, process energy. Just 1% to 2% of the lignin, derived from the sulfite pulp industry, is used in chemical conversion to produce lignosulfonates. Biorefining is a promising approach to promote the wider use of kraft lignin. However, using kraft lignin to produce high value-added products is a great challenge, due to its complex structure, low reactivity, and low solubility, which are factors that limit the lignin’s large-scale use in biorefineries. Recent studies show that kraft lignin can be used as lignosulfonates and dispersants, technical carbons, transportation fuels, bioplastics, and adhesives, but some technological hurdles must be overcome and several industrial tests must be developed to make these uses viable.

scholarly journals Green synthesis of silver nanoparticles using Tropaeolum majus: Phytochemical screening and antibacterial studies

2021 ◽  
Vol 10 (1) ◽  
pp. 85-94
Author(s):  
Saud Bawazeer ◽  
Abdur Rauf ◽  
Syed Uzair Ali Shah ◽  
Ahmed M. Shawky ◽  
Yahya S. Al-Awthan ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antioxidant Activity ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Aqueous Extract ◽  
Biological Action ◽  
Klebsiella Pneumonia ◽  
Atomic Force ◽  
Tropaeolum Majus

Abstract The green biosynthesis of metal nanoparticles of already explored phytomedicines has many advantages such as enhanced biological action, increased bioavailability, etc. In this direction, keeping in view the peculiar medicinal value of Tropaeolum majus L., we synthesized its silver nanoparticles (AgNPs) by adopting eco-friendly and cost-effective protocol by using methanolic and aqueous extract of T. majus. The synthesized AgNPs were characterized by using several techniques including UV spectroscopic analysis, FTIR analysis, and atomic force microscopy. The methanolic/aqueous extracts of T. majus and synthesized AgNPs were assessed for antioxidant potential and antimicrobial effect. The preliminary screening showed that the T. majus extracts have variety of reducing phytochemicals including tannins, terpenoids, flavonoids, and cardiac glycosides. The green synthesis of AgNPs was confirmed by the appearance of sharp peak at 430–450 nm in the UV-Visible spectra. The FTIR spectral analysis of extract and AgNPs exhibited that peaks at 2947.23, 2831.50, 2592.33, 2522.89, and 1,411 cm−1 disappeared in the spectra of FTIR spectra of the AgNPs, indicating carboxyl and hydroxyl groups are mainly accountable for reduction and stabilization of AgNPs. Atomic force microscopic scan of the synthesized AgNPs confirmed its cylindrical shape with size of 25 µm. The extracts and AgNPs were investigated for antioxidant potential by DPPH-free radical essay, which showed that aqueous extract has significant and dose-independent antioxidant activity; however, the synthesized AgNPs showed decline in antioxidant activity. The extracts and synthesized AgNPs were also evaluated for antibacterial activity against Klebsiella pneumonia, Staphylococcus aureus, and Bacillus subtilis. Neither extract nor AgNPs were active against Klebsiella pneumonia. The aqueous and methanolic extract exhibited inhibition against Bacillus subtilis and their synthesized AgNPs were active against Staphylococcus aureus. Our data concluded that the extracts of T. majus have necessary capping and reducing agents which make it capable to develop stable AgNPs. The aqueous extract of T. majus has potential antioxidant effect; however, the AgNPs did not enhance its free radical scavenging effect. The bacterial strains’ susceptibility of the extract and AgNPs was changed from Bacillus subtilis to Staphylococcus aureus, respectively. The biological action of AgNPs is changed in case of antibacterial activity which means that AgNPs might change the specificity of T. majus and likewise other drugs.

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