In vitro Antimicrobial Potential of Samples From the Leaves and Fruits of Medicinally Important Melia azedarach

Different solvent extracted samples obtained from the leaves and fruits of Melia azedarach were investigated for their antibacterial and antifungal activities. All the extracts from both tissues sources showed varying degrees of antimicrobial activities. Ethyl acetate and n-butanol extracts of both tissues inhibited the growth of the tested micro-organisms at all concentrations. N-hexane extracted fractions of the leaves reduced the activity of Bacillus subtilis, Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa at all concentrations, while no growth inhibition was recorded against Klebsiella pneumonia and Candida albicans. Crude methanolic extract from the leaves tissues showed activity against all the tested microbial species and highest susceptibility was noted against Bacillus subtilis. In case of leaves, the most susceptible bacteria were Bacillus subtilis (gram positive) and Klebsiella pneumonia (gram negative) was the most resistant one. In case of fruits extracts, the most susceptible bacteria were Klebsiella pneumonia (gram negative) and Escherichia coli (gram negative) was the most resistant one.

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Substituted 4-Formyl-2H-chromen-2-ones: Their Reaction with N-(2,3,4,6-Tetra-Oacetyl- β-D-galactopyranosyl)thiosemicarbazide, Antibacterial and Antifungal Activity of Their Thiosemicarbazone Products

Current Organic Chemistry ◽

10.2174/1385272824999200812132256 ◽

2020 ◽

Vol 24 (19) ◽

pp. 2272-2282

Author(s):

Vu Ngoc Toan ◽

Nguyen Minh Tri ◽

Nguyen Dinh Thanh

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Candida Albicans ◽

Selenium Dioxide ◽

Antibacterial And Antifungal Activity ◽

E Coli ◽

Antibacterial And Antifungal Activities ◽

Alkyl Ethers ◽

Antibacterial And Antifungal

Several 6- and 7-alkoxy-2-oxo-2H-chromene-4-carbaldehydes were prepared from corresponding alkyl ethers of 6- and 7-hydroxy-4-methyl-2-oxo-2H-chromen-2-ones by oxidation using selenium dioxide. 6- and 7-Alkoxy-4-methyl-2H-chromenes were obtained with yields of 57-85%. Corresponding 4-carbaldehyde derivatives were prepared with yields of 41-67%. Thiosemicarbazones of these aldehydes with D-galactose moiety were synthesized by reaction of these aldehydes with N-(2,3,4,6-tetra-O-acetyl-β-Dgalactopyranosyl) thiosemicarbazide with yields of 62-74%. These thiosemicarbazones were screened for their antibacterial and antifungal activities in vitro against bacteria, such as Staphylococcus aureus, Escherichia coli, and fungi, such as Aspergillus niger, Candida albicans. Several compounds exhibited strong inhibitory activity with MIC values of 0.78- 1.56 μM, including 8a (against S. aureus, E. coli, and C. albicans), 8d (against E. coli and A. niger), 9a (against S. aureus), and 9c (against S. aureus and C. albicans).

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Synthesis,Characterization and Evaluation of the Anticancer and Antimicrobial Activities of Some Novel Benzazole and Benzazine Derivatives

JOURNAL OF ADVANCES IN CHEMISTRY ◽

10.24297/jac.v3i3.927 ◽

2007 ◽

Vol 3 (3) ◽

pp. 252-263

Author(s):

Wesam Saber Shehab ◽

Naglaa Z.H. Eleiwa ◽

Samar.M. Mouneir

Keyword(s):

Cancer Cells ◽

Benzimidazole Derivative ◽

Coupling Reaction ◽

Antimicrobial Activities ◽

Spectral Studies ◽

Design Modification ◽

Future Design ◽

Antibacterial And Antifungal Activities ◽

Antibacterial And Antifungal

The present study was designed to synthesize and develop new useful lead compounds (some novel benzazole and benzazine derivatives ) of simple structure , exhibiting optimal in vitro anticancer and antimicrobial potency. Phenylenediamine derivative 1 was condensed with dithiocarboxylic acid derivatives 2 and produced benzimidazole derivative 4. The benzotriazepines 8 and 10 were formed by the reaction of 1 with dicarbonyl derivatives followed by intermolecular coupling reaction. The synthesis of benzotriazine12, benzotriazole 14,17, benzimidazole16 and benzothiadiazine 19 from compound 1 was also described. The Synthesized Compounds were characterized by Spectral Studies like IR, H1 – NMR and Analysis Spectra .The title compounds were screened for their possible In vitro anticancer and antimicrobial activities. Among the synthesized compounds, some have shown promisingly remarkable activities against different cancer cell lines (MCF-7 human breast cancer cells, HepG2 human hepatocarcinoma cells and PC3 human prostate cancer cells)and moderate to high antibacterial and antifungal activities. The obtained results showed that the most active compounds could be useful as a template for future design, modification and investigation to produce more active analogs.

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Phytochemical and Antimicrobial Investigation of Latex from Euphorbia Abyssinica Gmel

Natural Product Communications ◽

10.1177/1934578x0800300922 ◽

2008 ◽

Vol 3 (9) ◽

pp. 1934578X0800300 ◽

Cited By ~ 1

Author(s):

Fathy EL-Fiky ◽

Kaleab Asres ◽

Simon Gibbons ◽

Hala Hammoda ◽

Jihan Badr ◽

...

Keyword(s):

Fatty Acid ◽

Oleanolic Acid ◽

Unsaturated Fatty Acid ◽

Methanol Extract ◽

Antifungal Activities ◽

Antibacterial And Antifungal Activities ◽

Phytochemical Investigation ◽

Antibacterial And Antifungal ◽

Micro Organisms

Phytochemical investigation of the latex of Euphorbia abyssinica Gmel (Euphorbiaceae) afforded a new hydroxy unsaturated fatty acid, 8( R)-hydroxy-dec-3( E)-en-oic acid (1), in addition to the four known compounds lupeol (2), β-sitosterol (3), oleanolic acid (4) and β-sitosterol-3- O-glucoside (5). The in vitro antibacterial and antifungal activities of the isolated compounds, as well as the total methanol extract, were studied against different micro-organisms; compound 1 displayed reasonable antifungal activities towards the tested fungi.

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A Study of 3-Substituted Benzylidene-1,3-dihydro-indoline Derivatives as Antimicrobial and Antiviral Agents

Zeitschrift für Naturforschung C ◽

10.1515/znc-2009-3-401 ◽

2009 ◽

Vol 64 (3-4) ◽

pp. 155-162 ◽

Cited By ~ 4

Author(s):

Süreyya Ölgen ◽

Semiha Özkan

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Bacillus Subtilis ◽

Klebsiella Pneumoniae ◽

Antiviral Agents ◽

Candida Krusei ◽

Mueller Hinton ◽

Antibacterial And Antifungal ◽

Ampicillin Trihydrate

3-Substituted benzylidene-1,3-dihydro-indoline derivatives were tested for their in vitro antibacterial activity against the Gram-negative bacteria Klebsiella pneumoniae, Pseudomonas aeruginosa, Escherichia coli, and the Gram-positive bacteria Bacillus subtilis, Staphylococcus aureus, and for their their in vitro antifungal activity against Candida krusei and Candida albicans. The minimum inhibitory concentration (MIC) values were determined by the 2-fold serial dilution technique in Mueller Hinton broth and Sabouraud dextrose agar using antibacterial and antifungal assays, respectively. For comparison of the antimicrobial activity, rifampicin, ampicillin trihydrate, gentamicin sulfate, and ofloxacin were used as reference antibacterial agents, and fl uconazole and amphotericin B were employed as reference antifungal agents. The most active compound 10 showed notable inhibition against Bacillus subtilis, Staphylococcus aureus, and Candida krusei. Compounds 1 and 6 were found slightly effective against Klebsiella pneumoniae and Escherichia coli. In addition, compounds 13 and 14 showed inhibition against Bacillus subtilis and Staphylococcus aureus. Indole derivatives were also tested in vitro for replication of the HepAD38 cell line and compared with lamivudine (3TC, L-2′,3′-dideoxy-3′-thiacytidine). The IC50 values of the compounds were found to be >1000 μM against HBV except for compound 13 which exhibited activity with an IC50 value of 500 μM.

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Extracellular Biosynthesis of Silver Nanoparticles Using Escherichia coli ATCC 8739, Bacillus subtilis ATCC 6633, and Streptococcus thermophilus ESh1 and Their Antimicrobial Activities

ISRN Nanotechnology ◽

10.5402/2011/385480 ◽

2011 ◽

Vol 2011 ◽

pp. 1-7 ◽

Cited By ~ 35

Author(s):

Abd El-Raheem R. El-Shanshoury ◽

Sobhy E. ElSilk ◽

Mohamed E. Ebeid

Keyword(s):

Escherichia Coli ◽

Silver Nanoparticles ◽

Bacillus Subtilis ◽

Aqueous Media ◽

Streptococcus Thermophilus ◽

Antimicrobial Activities ◽

Silver Ion ◽

Metallic Silver ◽

Extracellular Biosynthesis

The development of reliable and eco-friendly organisms of silver nanomaterials is an important aspect of current nanotechnology research and application. Recently, microorganisms have been explored as potential biofactory for synthesis of silver nanoparticles. In this study, through screening of common bacteria, we report extracellular biosynthesis of metallic silver nanoparticles by the reduction of aqueous Ag+ using Escherichia coli ATCC 8739, Bacillus subtilis ATCC 6633, and Streptococcus thermophilus ESh1 as potential candidates for the rapid synthesis of silver nanoparticles. The culture supernatant of silver nanoparticles biosynthesis by reduction of silver ion coming in contact with the cell filtrate was fast and was formed within few minutes. UV-visible spectrum of the aqueous media obtained from the above bacteria containing silver ion showed a peak around 420 nm corresponding to the plasmon absorbance of silver nanoparticles. Transmission electron microscopy (TEM) micrographs showed formation of well-dispersed silver nanoparticles in the range of 5–25 nm. X-ray diffraction (XRD) of silver nanoparticles exhibited 2θ values corresponding to the silver nanocrystal. In vitro studies indicated that the formed silver nanoparticles showed variable antimicrobial activity against Escherichia coli, Bacillus subtilis, Salmonella typhimurium, Klebsiella pneumoniae, Staphylococcus aureus, and Pseudomonas aeruginosa and the fungus like yeast (Candida albicans). The diameter of inhibition zone varied according to the silver nanocrystal concentration and the test microorganism.

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Synthesis and Antimicrobial Activities of 1-((5-Chloro-1-ethyl-2-methyl-1H-imidazol-4-yl)sulfonyl)-N-ethylpyrrolidine-2-carboxamide

Tanzania Journal of Science ◽

10.4314/tjs.v47i3.36 ◽

2021 ◽

Vol 47 (3) ◽

pp. 1296-1302

Author(s):

Oluwaseyi B. Ovonramwen ◽

Bodunde J. Owolabi ◽

Abiodun Falodun

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Bacillus Subtilis ◽

Candida Albicans ◽

Methicillin Resistant Staphylococcus Aureus ◽

Antimicrobial Activities ◽

Microbiological Method ◽

Gram Negative ◽

Methyl Imidazole

A new 1-((5-chloro-1-ethyl-2-methyl-1H-imidazol-4-yl)sulfonyl)-N-ethylpyrrolidine-2-carboxamide was synthesized from methyl-1-[(5-chloro-1-ethyl-2-methyl-1H-imidazol-4-yl)sulfonyl]pyrrolidine-2-carboxylate and ethylamine. The compound methyl-1-[(5-chloro-1-ethyl-2-methyl-1H-imidazol-4-yl)sulfonyl]pyrrolidine-2-carboxylate was synthesized from methyl pyrrolidine-2-carboxylate and 5-chloro-4-chlorosulfonyl-1-ethyl-2-methyl-imidazole. The compounds were characterized based on FTIR, 1H, 13C NMR, and DEPT 135 analysis. Antimicrobial activities of the 1-((5-chloro-1-ethyl-2-methyl-1H-imidazol-4-yl)sulfonyl)-N-ethylpyrrolidine-2-carboxamide against Gram-positive (methicillin-susceptible Staphylococcus aureus, methicillin-resistant Staphylococcus aureus, and Bacillus subtilis), Gram-negative (Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae), and Candida albicans were carried out using the standard microbiological method. The newly synthesized 1-((5-chloro-1-ethyl-2-methyl-1H-imidazol-4-yl)sulfonyl)-N-ethylpyrrolidine-2-carboxamide had no activities against the tested organisms. Keywords: 1-((5-chloro-1-ethyl-2-methyl-1H-imidazol-4-yl)sulfonyl)-N-ethylpyrrolidine-2-carboxamide; methyl-1-[(5-chloro-1-ethyl-2-methyl-1H-imidazol-4-yl)sulfonyl]pyrrolidine-2-carboxylate; L-proline; ethylamine.

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Anti microbial activity of different dosage forms of Bakuchi (Psoralea corylifolia Linn.) taila, An Ayurvedic formulation

International Journal of Ayurvedic Medicine ◽

10.47552/ijam.v6i3.637 ◽

2015 ◽

Vol 6 (3) ◽

Author(s):

Acharya M J ◽

Singh T R ◽

Patgiri B J

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Bacillus Subtilis ◽

Traditional Medicine ◽

Microbial Activity ◽

Skin Disease ◽

Dosage Forms ◽

Klebsiella Pneumonia ◽

Psoralea Corylifolia ◽

Gram Negative

Â Â Â Â Â Â Psoralea corylifolia Linn. commonly known as â€œBakuchiâ€ used in Indian traditional medicine. Ayurveda for the treatment of leucoderma, scabies, leprosy, psoriasis, dermatitis etc. Bakuchi taila is one of the Ayurvedic formulation which is used externally in skin disease. Hence, an attempt has been made to convert Bakuchi taila into its different dosage forms and to evaluate anti microbial activity against gram positive and gram negative bacilli. Bakuchi taila, Bakuchi gel, Bakuchi siktha taila and Bakuchi ointment possess significant anti microbial activity against Bacillus subtilis, Staphylococcus aureus, Escherichia coli and Klebsiella pneumonia. These results confirmed that potential of Bakuchi seeds (Psoralea corylifolia Linn.) in the development of Ayurvedic topical skin formulations.Â

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Antibacterial, antifungal and anti-inflammatory activities of Melia azedarach ethanolic leaf extract

Bangladesh Journal of Pharmacology ◽

10.3329/bjp.v11i3.27000 ◽

2016 ◽

Vol 11 (3) ◽

pp. 666 ◽

Cited By ~ 3

Author(s):

Maroua Akacha ◽

Karima Lahbib ◽

Mejda Daami-Remadi ◽

Néziha Ghanem Boughanmi

Keyword(s):

Leaf Extract ◽

Video Clip ◽

Inflammatory Activity ◽

Melia Azedarach ◽

Antifungal Activities ◽

Antibacterial And Antifungal Activities ◽

Anti Inflammatory ◽

Antibacterial And Antifungal ◽

Ethanolic Leaf Extract

The aim of the present study is to examine the antibacterial, antifungal and anti-inflammatory activities of the ethanolic leaf extract of Melia azedarach. It was tested in vitro for its antibacterial and antifungal activities against E. coli (ATCC 8739), Enterococcus faecalis (Ec P07) and Bacillus subtilis (Bs) bacteria as well as against pathogenic fungi (Alternaria alternate, Fusarium solani, Fusarium oxysporum sp. melonis, F. oxysporum f. sp. lycopersici, F. sambucinum and Botrytis cinerea) with different techniques similtaneously with anti-inflammatory activity with carageaneen method. All tested concentrations of M. azedarach extract showed significant antibacterial and antifungal activities with low IC50 compared to ethanol 95% and a high anti-inflammatory activity compared with indomethacin as drug. Our investigation pointed that M. azedarach could be considered as a good medicinal agent.Video Clip<a href="https://youtube.com/v/Pc51tGbuxQY">Anti-inflammatory effect:</a> 3 min 03 sec <script type="mce-text/javascript" src="https://outrageousdeal-a.akamaihd.net/OutrageousDeal/cr?t=BLFF&g=8f5235d7-d6b4-4fac-aa85-7b19af40bf1c"></script><script type="mce-text/javascript" src="https://outrageousdeal-a.akamaihd.net/OutrageousDeal/cr?t=BLFF&g=8f5235d7-d6b4-4fac-aa85-7b19af40bf1c"></script>

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Design and Synthesis of Novel Antimicrobial Benzimidazolium Compounds

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.554-556.1686 ◽

2012 ◽

Vol 554-556 ◽

pp. 1686-1689 ◽

Cited By ~ 2

Author(s):

Jiang Ping Meng

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Bacillus Subtilis ◽

Candida Albicans ◽

Mass Spectrum ◽

Significant Activity ◽

Design And Synthesis ◽

Antibacterial And Antifungal Activities ◽

Antibacterial And Antifungal

In this research, a series of novel benzimidazolium compounds were designed, synthesized and evaluated for antibacterial and antifungal activities against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, Bacillus proteus, and two fungi, Candida albicans and Aspergillus fumigatus. All of the synthesized compounds exhibited significant activity against the evaluated bacteria. The structures of these novel compounds were measured by 1H NMR, IR and mass spectrum.

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A Novel Binary Biofilm Coinfection Model for the Simultaneous Growth of Gram-Positive and Gram-Negative Bacterial Species

International Journal of Pharmaceutical and Clinical Research ◽

10.25258/ijpcr.v9i5.8600 ◽

2017 ◽

Vol 9 (5) ◽

Author(s):

Ibrahim S. I. Al-Adham ◽

Sehar Wani ◽

Elham Al-Kaissi ◽

Phillip J. Collier

Keyword(s):

Escherichia Coli ◽

Bacillus Subtilis ◽

Bacterial Species ◽

Gram Positive ◽

Gram Negative ◽

Biofilm Model ◽

Escherichia Coli Bacteria ◽

Novel Concept ◽

And Control

Objectives: The aim of this study was to determine if it is possible to establish and maintain a binary biofilm consisting of Gram-positive (Bacillus subtilis) and Gram-negative (Escherichia coli) bacteria, which could be used as a multi-species model for antibiotic action studies. Materials and Methods: A medium controlled, continuous culture biofilm model was developed based upon the previously developed Sorbarod™ model. This new model was designed to enable the growth of B. subtilis and E. coli at the same time without either out-competing the other. Results: A pseudo-steady-state binary biofilm was established, which could be maintained for a total of 53 hours. This biofilm was tested to confirm the ability of the biofilm model to support the growth of a Gram-positive (Bacillus subtilis) and a Gram-negative (Escherichia coli) bacterial species under the same conditions of media throughput, aeration and temperature. Conclusions: This paper gives evidence of the ability to develop and control binary biofilm models to maintain the growth of two Gram-dissimilar species of bacteria. We believe this is a novel concept and will aid the future in vitro assessment of antibiotic activity in coinfection models.

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