Fermented Platycodon grandiflorum extract alleviates TNF-α/IFN-γ-induced inflammatory response in HaCaT cells and modulates immune balance on 1-chloro-2,4-dinitrobenzene-induced atopic dermatitis in NC/Nga mice

Plants of the genus Wikstroemia are traditionally used in China to treat various inflammatory diseases. The purpose of this study was to isolate the components of Wikstroemia ganpi (Siebold & Zucc.) Maxim., to evaluate their anti-atopic activities and to identify candidates with anti-atopic therapeutics. A total of 24 compounds were isolated by bioassay-guided separation, including one novel compound, which was tilianin 5-methyl ether. The anti-atopic activities of the isolated compounds were determined using TNF-α-treated RBL-2H3 cells and HaCaT cells. The mRNA expressions of IL-4, IL-6, GM-CSF, G-CSF and TRPV1 were reduced by luteolin 7-methyl ether. The study shows that the luteolin 7-methyl ether isolated from W. ganpi is a potential therapeutic agent for the treatment of atopic dermatitis.

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Modulation of the Gut Microbiota by Sihocheonggan-Tang Shapes the Immune Responses of Atopic Dermatitis

Frontiers in Pharmacology ◽

10.3389/fphar.2021.722730 ◽

2021 ◽

Vol 12 ◽

Author(s):

Jaemoo Chun ◽

So Min Lee ◽

You Mee Ahn ◽

Min-Gyung Baek ◽

Hana Yi ◽

...

Keyword(s):

Atopic Dermatitis ◽

Gut Microbiota ◽

Gut Microbiome ◽

Immunoglobulin E ◽

Therapeutic Potential ◽

Interleukin 4 ◽

Hacat Cells ◽

Necrosis Factor Alpha ◽

Tnf Α ◽

Ifn Γ

Atopic dermatitis (AD) is a chronic inflammatory skin disease characterized by complex immune dysregulation and closely related to the gut microbiome. The present study investigated the microbiome-mediated effect of Sihocheonggan-Tang (SHCGT) on AD-like symptoms induced by 2,4-dinitrochlorobenzene (DNCB) in BALB/c mice. DNCB was applied regularly to the ear and dorsal skin of BALB/c mice, and SHCGT was administered orally daily for 2 weeks. The composition of the gut microbiota was analyzed using 16S rRNA sequencing, and the effect of gut microbiome-derived metabolites, specifically short-chain fatty acids (SCFAs), was evaluated in tumor necrosis factor-alpha (TNF-α)- and interferon-gamma (IFN-γ)-treated HaCaT cells. SHCGT alleviated DNCB-induced symptoms of AD and the immune response to AD by decreasing the plasma immunoglobulin E level and splenic interleukin-4, interleukin-10, TNF-α, and IFN-γ levels. The gut microbiome composition and the damaged gut epithelial barrier in mice with AD were also significantly altered by SHCGT, and the reduced SCFA levels therein were elevated. We found that SFCAs directly inhibited the mRNA expression of IL-6 and ICAM-1 in TNF-α- and INF-γ-treated HaCaT cells. The finding that SHCGT regulates the gut microbiome and improves DNCB-induced AD in mice suggests that this herbal medicine has therapeutic potential in patients with AD.

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Jageum-Jung improves 2,4-dinitrochlorobenzene-induced atopic dermatitis-like skin lesions in mice and suppresses pro-inflammatory chemokine production by inhibiting TNF-α/IFN-γ-induced STAT-1 and NFκB signaling in HaCaT cells

Journal of Ethnopharmacology ◽

10.1016/j.jep.2018.04.016 ◽

2018 ◽

Vol 221 ◽

pp. 48-55 ◽

Cited By ~ 4

Author(s):

Ju-Hye Yang ◽

Hyun Ju Do ◽

Esther Lee ◽

Nam-Hui Yim ◽

Won-Kyung Cho ◽

...

Keyword(s):

Atopic Dermatitis ◽

Skin Lesions ◽

Hacat Cells ◽

Chemokine Production ◽

Tnf Α ◽

Ifn Γ

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Effects of Hovenia dulcis Thunb. extract and methyl vanillate on atopic dermatitis-like skin lesions and TNF-α/IFN-γ-induced chemokines production in HaCaT cells

Journal of Pharmacy and Pharmacology ◽

10.1111/jphp.12640 ◽

2016 ◽

Vol 68 (11) ◽

pp. 1465-1479 ◽

Cited By ~ 14

Author(s):

Sue Ji Lim ◽

Myungsuk Kim ◽

Ahmad Randy ◽

Eui Jeong Nam ◽

Chu Won Nho

Keyword(s):

Atopic Dermatitis ◽

Skin Lesions ◽

Hacat Cells ◽

Tnf Α ◽

Ifn Γ ◽

Hovenia Dulcis

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Inhibitory Effects of Urtica thunbergiana Ethanol Extract on Atopic Dermatitis-Induced NC/Nga Mice

Antioxidants ◽

10.3390/antiox9030197 ◽

2020 ◽

Vol 9 (3) ◽

pp. 197

Author(s):

Hien T.T. Ngo ◽

Minzhe Fang ◽

Eunson Hwang ◽

Yoosung Kim ◽

Bom Park ◽

...

Keyword(s):

Atopic Dermatitis ◽

Immunoglobulin E ◽

Elevated Serum ◽

Nuclear Factor ◽

Hacat Cells ◽

Tnf Α ◽

Ifn Γ ◽

Urtica Thunbergiana

Atopic dermatitis (AD) is a chronic, inflammatory skin disease that persists or repeatedly recurs in both childhood and adulthood. Urtica thunbergiana (UT) is an aroma herb with little-known pharmacological effects and anti-inflammatory activities against AD. This study investigated the immunomodulatory efficacy of 50% ethanol-extracted UT in necrosis factor-alpha/interferon-gamma (TNF-α/IFN-γ)-stimulated HaCaT cells in vitro and AD-Biostir-induced NC/Nga mice in vivo. The results showed that UT exhibits a dose-dependent increase in scavenged free radicals, reaching 76.0% ± 1.4% of scavenged 1,1-diphenyl-2-picrylhydrazyl at a concentration of 250 µg/mL. In addition, UT significantly downregulated the mRNA expression of the following pro-inflammatory cytokines and chemokines in TNF-α/IFN-γ-stimulated HaCaT cells: interleukin (IL)-6, IL-8, thymus- and activation-regulated chemokine, macrophage-derived chemokine, and regulated on activation normal T expressed and secreted. UT-treated HaCaT cells showed inhibition of the overexpression of chemokine-regulated signaling molecules, such as nuclear factor-kappa B, inhibitor of kappa B (IκBα), signal transducer and activator of transcription 1, and mitogen-activated protein kinases (MAPKs). UT dietary administration in AD-Biostir-induced NC/Nga mice treated and improved AD-like symptoms, such as scales, epidermal thickening, the dermatitis severity score, high trans-epidermal water loss, reduced skin hydration, increased mast cells, elevated serum immunoglobulin E levels, and an enlarged spleen. UT treatment inhibited the expression of phosphorylated forms of MAPKs, nuclear factor of activated T-cells 1, and regulator IκBα. It also upregulated filaggrin (FLG) production. Therefore, UT shows high anti-AD activity both in vitro and in vivo, and can be a useful anti-AD agent.

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Protective Effects of Kyungohkgo on Atopic Dermatitis in HaCaT Cells

Journal of the Korean Society of Cosmetology ◽

10.52660/jksc.2021.27.5.1286 ◽

2021 ◽

Vol 27 (5) ◽

pp. 1286-1292

Author(s):

Il-Joo Jo ◽

Mee-Ok Choi

Keyword(s):

Atopic Dermatitis ◽

Mrna Levels ◽

Dependent Manner ◽

Hacat Cells ◽

Protective Effects ◽

Concentration Dependent Manner ◽

Tnf Α ◽

Anti Oxidant ◽

Stat1 Signaling ◽

Ifn Γ

Kyungohkgo (KOG) is an oriental herbal medicine that has been used for its various pharmacological effects, which include anti-oxidant, anti-inflammatory and immuno-regulation activities. But its effects and mechanisms of anti-atopic dermatitis (AD) have not been elucidated. HaCaT cells were pre-treated with KOG for 1 h and stimulated with tumor necrosis factor (TNF)-α and interferon (IFN)-γ (10 ng/ml each). After 24 h, cells were harvested to measure the production of reactive oxygen species (ROS) and chemokines such as regulated upon activation, normal T cell expressed and secreted (RANTES/CCL5), Thymus and activation-regulated chemokine (TARC/CCL17) and macrophage-deraived chemokine (MDC/CCL22). To investigate the regulatory mechanisms of KOG, we also assessed the phosphorylation of signal transducer and activator of transcription (STAT1) signaling pathways in HaCaT cells. Treatment of KOG decreased the ROS production and mRNA levels of RANTES, TARC, MDC with a concentration dependent manner. In addition, KOG significantly inhibited TNF-α and IFN-γ induced phosphorylation of STAT1. This could indicate that the KOG shows anti-AD activity mainly through STAT1. Thus, we propose that KOG may be a promising anti-AD skin protector, which could suggest the clinical basis for cosmetics development.

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